为开发适宜于紫苏的SSR标记,对紫苏遗传多样性及种群划分进行研究,本研究采用已报道的紫苏及近源种中的66对SSR引物对不同来源的42份紫苏材料进行扩增。结果表明:15对引物在42份材料中具有多态性,共扩增获得54个条带,平均每对引物扩增条...为开发适宜于紫苏的SSR标记,对紫苏遗传多样性及种群划分进行研究,本研究采用已报道的紫苏及近源种中的66对SSR引物对不同来源的42份紫苏材料进行扩增。结果表明:15对引物在42份材料中具有多态性,共扩增获得54个条带,平均每对引物扩增条带3.6个,51个条带具有多态性,占扩增总条带的94.4%。使用UPGMA方法进行聚类分析,在SM=0.67处,供试紫苏分为2个主要聚类群。类群Ⅰ为紫苏原变种(Perilla frutescens var. frutescents),类群Ⅱ为回回苏变种(Perilla frutescens var. crispa),类群Ⅰ又可进一步划分为5个亚类群,各亚类群间表型具有一定的特征差异。本研究可为紫苏种质亲缘关系及资源鉴定研究提供参考依据。展开更多
Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears.Molecular markers linked to scab resistance gene is expected to be useful for improving pear.In this study,the F1 population deri...Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears.Molecular markers linked to scab resistance gene is expected to be useful for improving pear.In this study,the F1 population derived from the cross of ’Huangguan’ and ’Yali’ was analyzed genetically.The resistance of pear to scab was proved to be controlled by a single gene in a dominant manner.Bulked segregant analysis(BSA) was conducted to screen 64 fluorescent AFLP primer pairs.A marker designated as D3-365 was found to be linked to the resistant locus.Selective genotype linkage analysis showed that the genetic distance between the marker and the resistant locus was 14.9 cM.展开更多
文摘为开发适宜于紫苏的SSR标记,对紫苏遗传多样性及种群划分进行研究,本研究采用已报道的紫苏及近源种中的66对SSR引物对不同来源的42份紫苏材料进行扩增。结果表明:15对引物在42份材料中具有多态性,共扩增获得54个条带,平均每对引物扩增条带3.6个,51个条带具有多态性,占扩增总条带的94.4%。使用UPGMA方法进行聚类分析,在SM=0.67处,供试紫苏分为2个主要聚类群。类群Ⅰ为紫苏原变种(Perilla frutescens var. frutescents),类群Ⅱ为回回苏变种(Perilla frutescens var. crispa),类群Ⅰ又可进一步划分为5个亚类群,各亚类群间表型具有一定的特征差异。本研究可为紫苏种质亲缘关系及资源鉴定研究提供参考依据。
文摘Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears.Molecular markers linked to scab resistance gene is expected to be useful for improving pear.In this study,the F1 population derived from the cross of ’Huangguan’ and ’Yali’ was analyzed genetically.The resistance of pear to scab was proved to be controlled by a single gene in a dominant manner.Bulked segregant analysis(BSA) was conducted to screen 64 fluorescent AFLP primer pairs.A marker designated as D3-365 was found to be linked to the resistant locus.Selective genotype linkage analysis showed that the genetic distance between the marker and the resistant locus was 14.9 cM.
