SET(patient SE translocation,SET)基因,又名模板活化因子-1(template activating factor,TAF-1),因首次鉴定于伴有该基因染色体异位的未分化型白血病患者SE而命名。SET蛋白具有多种生物功能,可以通过影响组蛋白乙酰化、转录调节、核...SET(patient SE translocation,SET)基因,又名模板活化因子-1(template activating factor,TAF-1),因首次鉴定于伴有该基因染色体异位的未分化型白血病患者SE而命名。SET蛋白具有多种生物功能,可以通过影响组蛋白乙酰化、转录调节、核小体装配等,参与基因表达调控、翻译后修饰、细胞凋亡等多个生物过程,是重要的细胞因子。一些消化和血液系统肿瘤、生殖与神经系统的疾病伴有SET表达或亚细胞定位异常,提示SET与这些疾病的发生发展相关。SET基因转录剪切产生TAF-Iα和SET/TAF-Iβ2个亚型,而SET/TAF-Iβ可能发挥着SET蛋白的主要生物学作用。展开更多
嗜肝 DNA 病毒核衣壳装配是病毒复制周期的一个中心环节,需要3种病毒成分(前基因组 RNA、核心蛋白、多聚酶蛋白)特异和有次序地相互作用。研究表明:核心蛋白的氨基端具有自我组装核壳的能力,而羧基端具有结合核酸、稳定核壳的作用;多聚...嗜肝 DNA 病毒核衣壳装配是病毒复制周期的一个中心环节,需要3种病毒成分(前基因组 RNA、核心蛋白、多聚酶蛋白)特异和有次序地相互作用。研究表明:核心蛋白的氨基端具有自我组装核壳的能力,而羧基端具有结合核酸、稳定核壳的作用;多聚酶蛋白的4个区域均为介导前基因组 RNA 包装所需:前基因组 RNA 上存在的包装信号决定其选择性包装。本文最后讨论了嗜肝 DNA 病毒核衣壳装配研究的潜在应用价值。展开更多
Variable Charge X/Y (VCX/Y) is a human testis-specific gene family that localized on X and Y chromosomes. In this study, VCY protein was expressed in E. coli in the form of glutathione-S-transferase (GST) fusion prote...Variable Charge X/Y (VCX/Y) is a human testis-specific gene family that localized on X and Y chromosomes. In this study, VCY protein was expressed in E. coli in the form of glutathione-S-transferase (GST) fusion protein. With the purified fusion protein as antigen, the anti-GST-VCY antibody was generated and the localization of VCY protein in human testis was determined by immunohistochemistry. In the testis seminiferous epithelium. VCY proteins were highly expressed in nuclei of germ cells. Using propidium iodide staining and green fluorescent protein (GFP) tag technologies, VCY and VCX-8r proteins were mainly localized in the uucleoli of COST cells. In addition, the colocalization for VCY and VCX-8r in COS7 cells was also observed. With VCY cDNA as bait, a cDNA fragment of acidic ribosomal protein PO was obtained using yeast two-hybrid system. All the information above indicates that VCX/Y protein family might be involved in the regulation of ribosome assembly (hiring spermatogenesis.展开更多
类Y染色体编码睾丸特异蛋白基因家族(Testis-specific protein Y encoded-like,TSPYL)编码一类具有参与核小体装配,维持细胞活力状态功能的蛋白。这些蛋白在人体内分部广泛,并参与机体的多种生理病理过程。近期研究表明该类基因的变异...类Y染色体编码睾丸特异蛋白基因家族(Testis-specific protein Y encoded-like,TSPYL)编码一类具有参与核小体装配,维持细胞活力状态功能的蛋白。这些蛋白在人体内分部广泛,并参与机体的多种生理病理过程。近期研究表明该类基因的变异及其表观遗传学调控与某些发育性疾病和肿瘤的发生发展有较为密切的关系。通过研究TSPYL类基因的生物学特性和功能,以及与其相关疾病的关系,可为今后的基因靶向治疗提供新的方向和策略。展开更多
Using atomic force microscopy (AFM), the dynamic process of the in vitro nucleosome reconstitution followed by slow dilution from high salt to low salt was visualized. Data showed that the histone octamers were dissoc...Using atomic force microscopy (AFM), the dynamic process of the in vitro nucleosome reconstitution followed by slow dilution from high salt to low salt was visualized. Data showed that the histone octamers were dissociated from DNA at 1M NaCl. When the salt concentration was slowly reduced to 650 mMand 300 mM, the core histones bound to the naked DNA gradually. Once the salt concentration was reduced to 50 mM the classic 'beads-on-a-string' structure was clearly visualized. Furthermore, using the technique of the in vitro reconstitution ofnucleosome,the mono- and di- nucleosomes were assembled in vitro with both HS2core (-10681 to -10970 bp) and NCR2 (-372to -194 bp) DNA sequences in the 5'flanking sequence of human b-globin gene. Data revealed that HMG 1/2 and HMG 14/17 proteins binding to both DNA sequences are changeable following the assembly and disassembly of nucleosomes. We suggest that the changeable binding patterns of HMG 14/17 and HMG1/2 proteins with these regulatory elements may be critical in the process of nucleosome assembly, recruitment of chromatin-modifying activities, and the regulation of human b-globin gene expression.展开更多
A cell-free preparation obtained from extracts of activated Xenopus laevis eggs induced chromatin decondensation and nuclear formation from demembranated Xenopus sperm nuclei. Electron microscopy revealed that the rea...A cell-free preparation obtained from extracts of activated Xenopus laevis eggs induced chromatin decondensation and nuclear formation from demembranated Xenopus sperm nuclei. Electron microscopy revealed that the reassembled nucleus had a double-layered nuclear membrane, nuclear pore complexes, and decondensed chromatin etc. Indirect immunofluorescence analysis demonstrated the presence of lamina in newly assembled nuclei. Western-blotting results showed that lamin LII was present in egg extracts and in lamina of the reassembled nuclei which were previously reported to contain only eggderived lamin LIII.展开更多
文摘SET(patient SE translocation,SET)基因,又名模板活化因子-1(template activating factor,TAF-1),因首次鉴定于伴有该基因染色体异位的未分化型白血病患者SE而命名。SET蛋白具有多种生物功能,可以通过影响组蛋白乙酰化、转录调节、核小体装配等,参与基因表达调控、翻译后修饰、细胞凋亡等多个生物过程,是重要的细胞因子。一些消化和血液系统肿瘤、生殖与神经系统的疾病伴有SET表达或亚细胞定位异常,提示SET与这些疾病的发生发展相关。SET基因转录剪切产生TAF-Iα和SET/TAF-Iβ2个亚型,而SET/TAF-Iβ可能发挥着SET蛋白的主要生物学作用。
文摘嗜肝 DNA 病毒核衣壳装配是病毒复制周期的一个中心环节,需要3种病毒成分(前基因组 RNA、核心蛋白、多聚酶蛋白)特异和有次序地相互作用。研究表明:核心蛋白的氨基端具有自我组装核壳的能力,而羧基端具有结合核酸、稳定核壳的作用;多聚酶蛋白的4个区域均为介导前基因组 RNA 包装所需:前基因组 RNA 上存在的包装信号决定其选择性包装。本文最后讨论了嗜肝 DNA 病毒核衣壳装配研究的潜在应用价值。
基金supported by grants from the Special Fund for Major State Basic Research Project(No.G1999055901)National Natural Sciences Foundation of China(No.30240019)+1 种基金National High Technology Research and Technology of China(No.2001AA221131)State Ministry of Science and Technology Program(No.2002BA711A011.
文摘Variable Charge X/Y (VCX/Y) is a human testis-specific gene family that localized on X and Y chromosomes. In this study, VCY protein was expressed in E. coli in the form of glutathione-S-transferase (GST) fusion protein. With the purified fusion protein as antigen, the anti-GST-VCY antibody was generated and the localization of VCY protein in human testis was determined by immunohistochemistry. In the testis seminiferous epithelium. VCY proteins were highly expressed in nuclei of germ cells. Using propidium iodide staining and green fluorescent protein (GFP) tag technologies, VCY and VCX-8r proteins were mainly localized in the uucleoli of COST cells. In addition, the colocalization for VCY and VCX-8r in COS7 cells was also observed. With VCY cDNA as bait, a cDNA fragment of acidic ribosomal protein PO was obtained using yeast two-hybrid system. All the information above indicates that VCX/Y protein family might be involved in the regulation of ribosome assembly (hiring spermatogenesis.
文摘类Y染色体编码睾丸特异蛋白基因家族(Testis-specific protein Y encoded-like,TSPYL)编码一类具有参与核小体装配,维持细胞活力状态功能的蛋白。这些蛋白在人体内分部广泛,并参与机体的多种生理病理过程。近期研究表明该类基因的变异及其表观遗传学调控与某些发育性疾病和肿瘤的发生发展有较为密切的关系。通过研究TSPYL类基因的生物学特性和功能,以及与其相关疾病的关系,可为今后的基因靶向治疗提供新的方向和策略。
基金This work was supported by the National Natural Science Foundation of China(Grant No.39893320 and 39870378)the Foundation of the Chinese Academy of Sciences(Grant No.Kj982-j1-618).
文摘Using atomic force microscopy (AFM), the dynamic process of the in vitro nucleosome reconstitution followed by slow dilution from high salt to low salt was visualized. Data showed that the histone octamers were dissociated from DNA at 1M NaCl. When the salt concentration was slowly reduced to 650 mMand 300 mM, the core histones bound to the naked DNA gradually. Once the salt concentration was reduced to 50 mM the classic 'beads-on-a-string' structure was clearly visualized. Furthermore, using the technique of the in vitro reconstitution ofnucleosome,the mono- and di- nucleosomes were assembled in vitro with both HS2core (-10681 to -10970 bp) and NCR2 (-372to -194 bp) DNA sequences in the 5'flanking sequence of human b-globin gene. Data revealed that HMG 1/2 and HMG 14/17 proteins binding to both DNA sequences are changeable following the assembly and disassembly of nucleosomes. We suggest that the changeable binding patterns of HMG 14/17 and HMG1/2 proteins with these regulatory elements may be critical in the process of nucleosome assembly, recruitment of chromatin-modifying activities, and the regulation of human b-globin gene expression.
文摘A cell-free preparation obtained from extracts of activated Xenopus laevis eggs induced chromatin decondensation and nuclear formation from demembranated Xenopus sperm nuclei. Electron microscopy revealed that the reassembled nucleus had a double-layered nuclear membrane, nuclear pore complexes, and decondensed chromatin etc. Indirect immunofluorescence analysis demonstrated the presence of lamina in newly assembled nuclei. Western-blotting results showed that lamin LII was present in egg extracts and in lamina of the reassembled nuclei which were previously reported to contain only eggderived lamin LIII.
