Populus euphratica Oliv. is of high salinity tolerance and used as a model species for investigating molecular mechanisms of trees' responses to salt stress. In the work presented here we found that calli of P. eu...Populus euphratica Oliv. is of high salinity tolerance and used as a model species for investigating molecular mechanisms of trees' responses to salt stress. In the work presented here we found that calli of P. euphratica grew more rapidly and accumulated less Na+, but more K+, under salt stress than those of salt-sensitive poplar, Populus hopeiensis. Different types of Na+/H+ antiporters (SOS1, NhaD1 and NHX1) were isolated from P. euphratica; all of these genes have been shown to play important roles in plant salt tolerance mechanism in previous studies. Expression profiles of these three genes were compared between P. hopeiensis and P. euphratica in the presence and absence of salt stress by real-time PCR. The three genes were induced in both P. euphratica and P. hopeiensis by salt. Transcript levels of PeNHX1 were lower in P. euphratica than in P. hopeiensis under 150 mM NaCl stress. In addition, transcript levels of PeNhaD1 were lower, while PeSOS1 were higher in P. euphratica than in P. hopeiensis under both stressed and unstressed conditions. The results indicated that P. euphratica up-regulates different genes and consistently maintains both effluxes of Na+ and high K+ levels. Our data suggests that differences in gene expression patterns may contribute to the dif-ference in salt tolerance between these two poplars.展开更多
[Objective] This study aimed to map quantitative tarit loci (QTLs) associated with salt tolerance of maize inbred line at seedling stage. [Method] The recombinant inbred line (RIL) F7 including 171 plants were develop...[Objective] This study aimed to map quantitative tarit loci (QTLs) associated with salt tolerance of maize inbred line at seedling stage. [Method] The recombinant inbred line (RIL) F7 including 171 plants were developed by single seed descent procedure from a combination, Huangzaosi×Mo17, and used to map QTLs associated with salt tolerance, based on the constructed genetic map of SSR markers. [Result]A linkage map consisting of 81 SSR markers loci from 10 chromosomes (1 428.3 cM in total length, with an average distance of 17.63 cM between two neighbouring loci) was constructed. Six QTLs associated significantly with salt tolerance were detected at chromosomes 1, 5 and 6. [Conclusion] This study is extremely significant for better understanding salt tolerance-related genes, the genes' location and cloning, salt tolerance mechanism and the marker-assisted selection of salt tolerant maize.展开更多
基金NSFC (30800865, 40801001)the Education Ministry of China (NCET-05-0886)China Postdoctoral Science Foundation (20070420758)
文摘Populus euphratica Oliv. is of high salinity tolerance and used as a model species for investigating molecular mechanisms of trees' responses to salt stress. In the work presented here we found that calli of P. euphratica grew more rapidly and accumulated less Na+, but more K+, under salt stress than those of salt-sensitive poplar, Populus hopeiensis. Different types of Na+/H+ antiporters (SOS1, NhaD1 and NHX1) were isolated from P. euphratica; all of these genes have been shown to play important roles in plant salt tolerance mechanism in previous studies. Expression profiles of these three genes were compared between P. hopeiensis and P. euphratica in the presence and absence of salt stress by real-time PCR. The three genes were induced in both P. euphratica and P. hopeiensis by salt. Transcript levels of PeNHX1 were lower in P. euphratica than in P. hopeiensis under 150 mM NaCl stress. In addition, transcript levels of PeNhaD1 were lower, while PeSOS1 were higher in P. euphratica than in P. hopeiensis under both stressed and unstressed conditions. The results indicated that P. euphratica up-regulates different genes and consistently maintains both effluxes of Na+ and high K+ levels. Our data suggests that differences in gene expression patterns may contribute to the dif-ference in salt tolerance between these two poplars.
基金Supported by Project of Key Opening Laboratory of Physiology and Ecology of Crop in Cold Terra of Agriculture MinistryProject of the Department of Education of Heilongjiang Province (11521196)
文摘[Objective] This study aimed to map quantitative tarit loci (QTLs) associated with salt tolerance of maize inbred line at seedling stage. [Method] The recombinant inbred line (RIL) F7 including 171 plants were developed by single seed descent procedure from a combination, Huangzaosi×Mo17, and used to map QTLs associated with salt tolerance, based on the constructed genetic map of SSR markers. [Result]A linkage map consisting of 81 SSR markers loci from 10 chromosomes (1 428.3 cM in total length, with an average distance of 17.63 cM between two neighbouring loci) was constructed. Six QTLs associated significantly with salt tolerance were detected at chromosomes 1, 5 and 6. [Conclusion] This study is extremely significant for better understanding salt tolerance-related genes, the genes' location and cloning, salt tolerance mechanism and the marker-assisted selection of salt tolerant maize.
