[Objective] This study aimed to select microalgae species which are capable to effectively remove arsenic contamination from water under natural conditions. [Method] Four microalgae species [Chlorella sp. （zfsaia）, ...[Objective] This study aimed to select microalgae species which are capable to effectively remove arsenic contamination from water under natural conditions. [Method] Four microalgae species [Chlorella sp. （zfsaia）, Chlorella minata, Chlorella vulgaris and Selenastrum capricormulum] were used as experimental materials and cultured with six different concentrations of As （Ⅲ） （0.5, 1.0, 2.0, 5.0, 10.0, 20.0 mg/L）. Biomass, chlorophyll a content and other physiological indicators were determined to investigate the arsenic tolerance and biosorption of four microalgae species. [Result] Chlorella sp. is sensitive to arsenic toxicity, its growth was inhibited when arsenic concentration exceeded 10 mg/L, with an EC 50 of 17.32 mg/L; when the arsenic concentration was 0-20 mg/L, growth of S.c, ww1 and C.v was not affected, which showed relatively high tolerance to arsenic, with arsenic removal rates of 77.02% , 72.18% and 81.36% respectively after 24 h. [Conclusion] This study indicates that microalgae have good application prospects for processing arsenic wastewater and being indicator plants of arsenic wastewater.展开更多
Objectives： An open-label prospective, combined basic and clinical controlled study was done to investigate the effects of biological therapy using rituximab, and cytotoxic drug treatment with methotrexate on morphol...Objectives： An open-label prospective, combined basic and clinical controlled study was done to investigate the effects of biological therapy using rituximab, and cytotoxic drug treatment with methotrexate on morphology and quantifitiation of chromosomes in rheumatoid arthritis patients. Methods： This study is follow-up of a prior publication, with new observations in comparison with control subjects. A total of 16 subjects were divided into two groups. Group I comprised 8 seropositive rheumatoid arthritis patients who were analysed for the primary end point of possible cytotoxic effects of rituximab and methotrexate. Group II included 8 healthy individuals who served as controls. Assessment was done before treatment with rituximab, and 4 weeks after initiation of therapy. Patients were randomly assigned to receive infusion of rituximab in a full dose of 2.0 g divided into two doses of 1.0 g on days I and 15. The lymphocytes from periphereal blood was cultured by the Moorhead method. Results： Normal male and female Karyograms were observed after full courses of therapy with rituximab. In one female patient who had been receiving longstanding cytotoxic therapy with methotrexate, 2% of chromosomal mitosis showed structural abnormalities. Following the discontinuation of methotrexate and the administration of rituximab, her karyogram became normal. Conclusion： The results from this study indicated that rituximab therapy was safe for the number and structure of human chromosomes, while methotrexate showed chromosomal aberration in one female RA patient. After discontinuation of this longstanding treatment, the karyogram of the same patient returned to normal.展开更多
Breast cancer is one of the malignant tumors with the highest morbidity and mortality. It is helpful to reduce the rate of tumor recurrence and metastasis by treating breast cancer with adjuvant chemotherapy, so as to...Breast cancer is one of the malignant tumors with the highest morbidity and mortality. It is helpful to reduce the rate of tumor recurrence and metastasis by treating breast cancer with adjuvant chemotherapy, so as to increase the cure rate or survival of patients. In recent years, liposomes have been regarded as a kind of new carrier for targeted drugs. Being effective for enhancing drug efficacy and reducing side effects, they have been widely used for devel- oping anticancer drugs. As a kind of anthracycline with high anticancer activity, doxorubicin can treat or alleviate a variety of malignant tumors effectively when it is used on its own or in combination with other anticancer drugs~ Alt- hough liposomal doxorubicin has been extensively used in the adjuvant chemotherapy of breast cancer, its exact therapeutic efficacy and side effects have not been definitely proven. Various clinical studies have adopted different combined regimes, dosages, and staging, so their findings differ to certain extent. This paper reviews the clinical application of liposomal doxorubicin in the adjuvant chemotherapy of breast cancer and illustrates therapeutic effects and side effects of pegylated liposomal doxorubicin （PLD） and non-PLD （NPLD） in clinical research, in order to discuss the strategies for applying these drugs in such adjuvant chemotherapy, looking forward to providing references for related research and clinical treatment in terms of dosage, staging, combined regimes, and analysis methods and so on.展开更多
Dissolved organic matter(DOM) is a group of compounds that have complex chemical structures and multiple interactions with their surrounding materials. More than one trillion tons of DOM are stocked in the world's...Dissolved organic matter(DOM) is a group of compounds that have complex chemical structures and multiple interactions with their surrounding materials. More than one trillion tons of DOM are stocked in the world's aquatic ecosystems. DOM is a very important part of aquatic ecosystem productivity and plays a crucial role in global carbon cycling. DOM has rich environmental behaviors and effects such as influencing the bioavailability of contaminants, serving as an important inducer of reactive oxygen species(ROS), and protecting aquatic organisms from the harm of dangerous ultraviolet radiation. There have been many systematic studies on the composition, structure, and sources of DOM because such studies are much easier to conduct than studies on the environmental behaviors and effects of DOM. Due to many factors, the research systems of DOM's environmental behaviors and effects are still being developed and have become a hotspot of environmental science. This review paper focuses on some critical progress, problems, and trends of DOM's environmental behaviors and effects in aquatic ecosystems, including mutual exchange mechanisms between DOM and particulate organic matter(POM) with influencing factors, photochemical behaviors of DOM especially inducing ROS, binding interactions between DOM and anthropogenic organic contaminants(AOC), interactions between DOM and microorganisms, effects of DOM on pollutants' bioavailability, ecotoxicity, and ecological risks. Hopefully, this paper will contribute to a more systematic understanding of the DOM environmental behaviors and effects and to promoting further relevant studies.展开更多
Chemotherapy as an effective cancer treatment technique has been widely used in tumor therapy. However, it is still a challenge to overcome the serious side effects of chemotherapy, especially for its myelotoxicity. H...Chemotherapy as an effective cancer treatment technique has been widely used in tumor therapy. However, it is still a challenge to overcome the serious side effects of chemotherapy, especially for its myelotoxicity. Here we report a novel strategy using the water soluble gadofullerene nanocrystals（GFNCs） to protect against chemotherapy injury in hepatocarcinoma bearing mice, which was induced by the commonly chemotherapeutic agent cyclophosphamide（CTX）.The GFNCs were revealed to specifically accumulate in the bone marrow after intravenously injecting to mice and they exhibited excellent radical scavenging function, resulting in a prominent increase of mice blood cells and pathological improvements of the primary organs in the GFNCs（15 mg kg-（-1））treated mice after the CTX（60 mg kg-（-1）） therapy. Moreover,the GFNCs maintained and even strengthened the antineoplastic activity of the CTX agent. Therefore, the GFNCs would be the promising chemoprotective agents in chemotherapy based on their high efficiency, low toxicity and metabolizable property.展开更多
基金Supported by Natural Science Foundation of Guangdong Province (10151064101000041)Ph.D. Funds from Ministry of Education of China(20090172120032)~~
文摘[Objective] This study aimed to select microalgae species which are capable to effectively remove arsenic contamination from water under natural conditions. [Method] Four microalgae species [Chlorella sp. （zfsaia）, Chlorella minata, Chlorella vulgaris and Selenastrum capricormulum] were used as experimental materials and cultured with six different concentrations of As （Ⅲ） （0.5, 1.0, 2.0, 5.0, 10.0, 20.0 mg/L）. Biomass, chlorophyll a content and other physiological indicators were determined to investigate the arsenic tolerance and biosorption of four microalgae species. [Result] Chlorella sp. is sensitive to arsenic toxicity, its growth was inhibited when arsenic concentration exceeded 10 mg/L, with an EC 50 of 17.32 mg/L; when the arsenic concentration was 0-20 mg/L, growth of S.c, ww1 and C.v was not affected, which showed relatively high tolerance to arsenic, with arsenic removal rates of 77.02% , 72.18% and 81.36% respectively after 24 h. [Conclusion] This study indicates that microalgae have good application prospects for processing arsenic wastewater and being indicator plants of arsenic wastewater.
文摘Objectives： An open-label prospective, combined basic and clinical controlled study was done to investigate the effects of biological therapy using rituximab, and cytotoxic drug treatment with methotrexate on morphology and quantifitiation of chromosomes in rheumatoid arthritis patients. Methods： This study is follow-up of a prior publication, with new observations in comparison with control subjects. A total of 16 subjects were divided into two groups. Group I comprised 8 seropositive rheumatoid arthritis patients who were analysed for the primary end point of possible cytotoxic effects of rituximab and methotrexate. Group II included 8 healthy individuals who served as controls. Assessment was done before treatment with rituximab, and 4 weeks after initiation of therapy. Patients were randomly assigned to receive infusion of rituximab in a full dose of 2.0 g divided into two doses of 1.0 g on days I and 15. The lymphocytes from periphereal blood was cultured by the Moorhead method. Results： Normal male and female Karyograms were observed after full courses of therapy with rituximab. In one female patient who had been receiving longstanding cytotoxic therapy with methotrexate, 2% of chromosomal mitosis showed structural abnormalities. Following the discontinuation of methotrexate and the administration of rituximab, her karyogram became normal. Conclusion： The results from this study indicated that rituximab therapy was safe for the number and structure of human chromosomes, while methotrexate showed chromosomal aberration in one female RA patient. After discontinuation of this longstanding treatment, the karyogram of the same patient returned to normal.
基金Project supported by the"151"Excellent Middle-Aged and Talented Young Persons of Zhejiang Province,China(No.2010382)the Key Platform Technological Project of Zhejiang Medical Science and Hygiene(No.2016ZDB003),China
文摘Breast cancer is one of the malignant tumors with the highest morbidity and mortality. It is helpful to reduce the rate of tumor recurrence and metastasis by treating breast cancer with adjuvant chemotherapy, so as to increase the cure rate or survival of patients. In recent years, liposomes have been regarded as a kind of new carrier for targeted drugs. Being effective for enhancing drug efficacy and reducing side effects, they have been widely used for devel- oping anticancer drugs. As a kind of anthracycline with high anticancer activity, doxorubicin can treat or alleviate a variety of malignant tumors effectively when it is used on its own or in combination with other anticancer drugs~ Alt- hough liposomal doxorubicin has been extensively used in the adjuvant chemotherapy of breast cancer, its exact therapeutic efficacy and side effects have not been definitely proven. Various clinical studies have adopted different combined regimes, dosages, and staging, so their findings differ to certain extent. This paper reviews the clinical application of liposomal doxorubicin in the adjuvant chemotherapy of breast cancer and illustrates therapeutic effects and side effects of pegylated liposomal doxorubicin （PLD） and non-PLD （NPLD） in clinical research, in order to discuss the strategies for applying these drugs in such adjuvant chemotherapy, looking forward to providing references for related research and clinical treatment in terms of dosage, staging, combined regimes, and analysis methods and so on.
基金supported by the National Project for Water Pollution Control in China(Grant No.2012ZX07103-002)the National Natural Science Foundation of China(Grant Nos.4150308341271462&41030529)
文摘Dissolved organic matter(DOM) is a group of compounds that have complex chemical structures and multiple interactions with their surrounding materials. More than one trillion tons of DOM are stocked in the world's aquatic ecosystems. DOM is a very important part of aquatic ecosystem productivity and plays a crucial role in global carbon cycling. DOM has rich environmental behaviors and effects such as influencing the bioavailability of contaminants, serving as an important inducer of reactive oxygen species(ROS), and protecting aquatic organisms from the harm of dangerous ultraviolet radiation. There have been many systematic studies on the composition, structure, and sources of DOM because such studies are much easier to conduct than studies on the environmental behaviors and effects of DOM. Due to many factors, the research systems of DOM's environmental behaviors and effects are still being developed and have become a hotspot of environmental science. This review paper focuses on some critical progress, problems, and trends of DOM's environmental behaviors and effects in aquatic ecosystems, including mutual exchange mechanisms between DOM and particulate organic matter(POM) with influencing factors, photochemical behaviors of DOM especially inducing ROS, binding interactions between DOM and anthropogenic organic contaminants(AOC), interactions between DOM and microorganisms, effects of DOM on pollutants' bioavailability, ecotoxicity, and ecological risks. Hopefully, this paper will contribute to a more systematic understanding of the DOM environmental behaviors and effects and to promoting further relevant studies.
基金financially supported by the National Natural Science Foundation of China(51472248,51372251 and 51502301)the National Major Scientific Instruments and Equipments Development Project(ZDYZ2015-2)the Key Research Program of the Chinese Academy of Sciences(QYZDJ-SSW-SLH025,KGZD-EWT02 and XDA09030302)
文摘Chemotherapy as an effective cancer treatment technique has been widely used in tumor therapy. However, it is still a challenge to overcome the serious side effects of chemotherapy, especially for its myelotoxicity. Here we report a novel strategy using the water soluble gadofullerene nanocrystals（GFNCs） to protect against chemotherapy injury in hepatocarcinoma bearing mice, which was induced by the commonly chemotherapeutic agent cyclophosphamide（CTX）.The GFNCs were revealed to specifically accumulate in the bone marrow after intravenously injecting to mice and they exhibited excellent radical scavenging function, resulting in a prominent increase of mice blood cells and pathological improvements of the primary organs in the GFNCs（15 mg kg-（-1））treated mice after the CTX（60 mg kg-（-1）） therapy. Moreover,the GFNCs maintained and even strengthened the antineoplastic activity of the CTX agent. Therefore, the GFNCs would be the promising chemoprotective agents in chemotherapy based on their high efficiency, low toxicity and metabolizable property.
