Objective: To evaluate the e?ect of the prepared leukemia vaccine on the cytotoxicity of macrophage of C57BL/6 mice. Methods: The model of mice bearing leukemia was established and three types ...Objective: To evaluate the e?ect of the prepared leukemia vaccine on the cytotoxicity of macrophage of C57BL/6 mice. Methods: The model of mice bearing leukemia was established and three types of leukemia vaccine were prepared before they were administered on the mice respectively. The cy- totxicity of M? derived from the mice was measured after the active immunotherapy or prevention for 2–4 weeks later by using MTT colorimetric method and compared with the control group. Results: (1) With the growth of leukemia cells in the mice, the cytotoxicity of M? was seriously depressed; (2) The administration of leukemia vaccine prepared from inactivated leukemic cells, incomplete Freund’s adjuvant (IFA) and cytokines, such as rGM-CSF, rIL-2 and rIL-6, promoted the cellular immunity of mice bearing leukemia, more e?cient than leukemia vaccine from either inactivated leukemic cells and IFA or inactive leukemic cells per se. Conclusion: The leukemia vaccine prepared with inactive leukemic cells, IFA and cytokines could activate the non-speci?c cellular immunity such as M?, as well as, antigen present, immune surveillance and so on, and this activation constructed a base for further activate T lymphocyte. Naturally, this will certainly have a promising future in the therapy against hematopietic tumor.展开更多
Herein, a multiwalled carbon nanotubes(MWNT)-based colorimetric probe was designed to discover and monitor the level of apolipoprotein-L1(Apo L1) in lumbar disc herniation(LDH) patients. Apo L1 could be easily found i...Herein, a multiwalled carbon nanotubes(MWNT)-based colorimetric probe was designed to discover and monitor the level of apolipoprotein-L1(Apo L1) in lumbar disc herniation(LDH) patients. Apo L1 could be easily found in human serum of the LDH group, but not obviously expressed in the normal control group(Ctrl), spine spondylolisthesis(SSP) group, spinal fracture(SFR) group, and spine scoliosis(SSC) group. Furthermore, the as-prepared MWNT-based probe was also used to track the recovery of LDH patients who have successful surgery operation. The P value of early diagnosis and recovery monitoring was <0.001 and >0.05 for the proposed method and a conventional enzyme-linked immunosorbent assay(ELISA), respectively, suggesting this detection strategy had significant differences compared with the traditional ELISA. All experimental results showed that Apo L1 might be a potential biomarker for early diagnosis of LDH. This proposed detection strategy has a potential application in discovering new biomarkers of diseases.展开更多
文摘Objective: To evaluate the e?ect of the prepared leukemia vaccine on the cytotoxicity of macrophage of C57BL/6 mice. Methods: The model of mice bearing leukemia was established and three types of leukemia vaccine were prepared before they were administered on the mice respectively. The cy- totxicity of M? derived from the mice was measured after the active immunotherapy or prevention for 2–4 weeks later by using MTT colorimetric method and compared with the control group. Results: (1) With the growth of leukemia cells in the mice, the cytotoxicity of M? was seriously depressed; (2) The administration of leukemia vaccine prepared from inactivated leukemic cells, incomplete Freund’s adjuvant (IFA) and cytokines, such as rGM-CSF, rIL-2 and rIL-6, promoted the cellular immunity of mice bearing leukemia, more e?cient than leukemia vaccine from either inactivated leukemic cells and IFA or inactive leukemic cells per se. Conclusion: The leukemia vaccine prepared with inactive leukemic cells, IFA and cytokines could activate the non-speci?c cellular immunity such as M?, as well as, antigen present, immune surveillance and so on, and this activation constructed a base for further activate T lymphocyte. Naturally, this will certainly have a promising future in the therapy against hematopietic tumor.
基金supported by the National Basic Research Program of China(2012CB933301)the Key Discipline Construction Project of Pudong Health Bureau of Shanghai(PWZx2014-02)+1 种基金the Sci-tech Support Plan of Jiangsu Province(BE2014719)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Herein, a multiwalled carbon nanotubes(MWNT)-based colorimetric probe was designed to discover and monitor the level of apolipoprotein-L1(Apo L1) in lumbar disc herniation(LDH) patients. Apo L1 could be easily found in human serum of the LDH group, but not obviously expressed in the normal control group(Ctrl), spine spondylolisthesis(SSP) group, spinal fracture(SFR) group, and spine scoliosis(SSC) group. Furthermore, the as-prepared MWNT-based probe was also used to track the recovery of LDH patients who have successful surgery operation. The P value of early diagnosis and recovery monitoring was <0.001 and >0.05 for the proposed method and a conventional enzyme-linked immunosorbent assay(ELISA), respectively, suggesting this detection strategy had significant differences compared with the traditional ELISA. All experimental results showed that Apo L1 might be a potential biomarker for early diagnosis of LDH. This proposed detection strategy has a potential application in discovering new biomarkers of diseases.
