AIM: To develop a GC MS method for the study of pharmacokinetics of gemfibrozil in healthy human body. METHODS: A 25 m×0 2 mm ID HP 5 silica capillary column was used. The carrier gas was helium. The internal sta...AIM: To develop a GC MS method for the study of pharmacokinetics of gemfibrozil in healthy human body. METHODS: A 25 m×0 2 mm ID HP 5 silica capillary column was used. The carrier gas was helium. The internal standard was ibuprofen. After acidification with 3 mol·L -1 HCl solution, the plasma was extracted with n hexane — dichloromethane (3∶1) and then reacted with bis (trimethylsilyl) trifluoroacetamide (BSTFA). RESULTS: A good linearity was obtained from 0 4 to 60 0 μg·mL -1 of gemfibrozil in human plasma (γ=0 9992). The detection limit of gemfibrozil in plasma was 0 1 μg·mL -1 . The average recovery was 96 5%. The pharmacokinetics of gemfibrozil was determined by this GC MS method following a single oral dose of 600 mg gemfibrozil capsule given to each of 10 volunteers. The results showed that the plasma concentration time courses conformed to one compartment model. CONCLUSION: The established GC MS method was found to be a good method for determination of gemfibrozil in human plasma. The method was precise and sensitive.展开更多
文摘AIM: To develop a GC MS method for the study of pharmacokinetics of gemfibrozil in healthy human body. METHODS: A 25 m×0 2 mm ID HP 5 silica capillary column was used. The carrier gas was helium. The internal standard was ibuprofen. After acidification with 3 mol·L -1 HCl solution, the plasma was extracted with n hexane — dichloromethane (3∶1) and then reacted with bis (trimethylsilyl) trifluoroacetamide (BSTFA). RESULTS: A good linearity was obtained from 0 4 to 60 0 μg·mL -1 of gemfibrozil in human plasma (γ=0 9992). The detection limit of gemfibrozil in plasma was 0 1 μg·mL -1 . The average recovery was 96 5%. The pharmacokinetics of gemfibrozil was determined by this GC MS method following a single oral dose of 600 mg gemfibrozil capsule given to each of 10 volunteers. The results showed that the plasma concentration time courses conformed to one compartment model. CONCLUSION: The established GC MS method was found to be a good method for determination of gemfibrozil in human plasma. The method was precise and sensitive.
