Aim To strdy the separation of native amino acids using capillary zone electro- pboresis (CZE) with indirect ultraviolet detecition. Methods 13 native amino acids were sepa- rated by capillary electrophoresis with ind...Aim To strdy the separation of native amino acids using capillary zone electro- pboresis (CZE) with indirect ultraviolet detecition. Methods 13 native amino acids were sepa- rated by capillary electrophoresis with indirect detection . The experiments were carried out with homemade CE apparatus under the following operating conditior conditions: a fused-silica capillary col- umn of 50.0cm effect length and of 75m i.d. was used. 7 organic acids were used as BGAE, and a positive potential of separation in CZE with indirect detection. After optimizing for l3 native amino acids were established. Conclusion The choice of BGAe is an important factor influencing the efficiency of separation in CZE with indiect detection .After optimizing the separation conditions a baseline separation for 13 native amino acids is obtained.展开更多
The myosin heavy chain(MyHC)is one of the major structural and contracting proteins of muscle.We have isolated the cDNA clone encoding MyHC of the grass carp,Ctenopharyngodon idella. The sequence comprises 5 934 bp,in...The myosin heavy chain(MyHC)is one of the major structural and contracting proteins of muscle.We have isolated the cDNA clone encoding MyHC of the grass carp,Ctenopharyngodon idella. The sequence comprises 5 934 bp,including a 5 814 bp open reading frame encoding an amino acid sequence of 1 937 residues.The deduced amino acid sequence showed 69%homology to rabbit fast skeletal MyHC and 73%–76%homology to the MyHCs from the mandarin fish,walleye pollack,white croaker,chum salmon,and carp.The putative sequences of subfragment-1 and the light meromyosin region showed 61.4%–80%homology to the corresponding regions of other fish MyHCs.The tissue-specific and developmental stage-specific expressions of the MyHC gene were analyzed by quantitative real-time PCR.The MyHC gene showed the highest expression in the muscles compared with the kidney,spleen and intestine.Developmentally,there was a gradual increase in MyHC mRNA expression from the neural formation stage to the tail bud stage.The highest expression was detected in hatching larva.Our work on the MyHC gene from the grass carp has provided useful information for fish molecular biology and fish genomics.展开更多
Photodissociation of p-aminobenzoic acid at 266 nm was investigated by probing the nascent OH photoproduct employing the laser-induced fluorescence technique. It was found that the nascent OH radical was vibrationally...Photodissociation of p-aminobenzoic acid at 266 nm was investigated by probing the nascent OH photoproduct employing the laser-induced fluorescence technique. It was found that the nascent OH radical was vibrationally cold and its rotational state distribution conformed to be a Boltzmann behavior, characterized by a rotational temperature of 1040±110 K. The rotational energy of OH was determined to be 8.78±0.84 kJ/mol. Between the two spinorbit states of OH, ^2Ⅱ3/2 and ^2Ⅱ1/2, the former was found to be preferentially populated. The distribution of the II（A＇） state for the A-doublet was dominant. Finally, a probable mechanism for the formation of OH produced from the photodissociation of p-aminobenzoic acid is discussed.展开更多
文摘Aim To strdy the separation of native amino acids using capillary zone electro- pboresis (CZE) with indirect ultraviolet detecition. Methods 13 native amino acids were sepa- rated by capillary electrophoresis with indirect detection . The experiments were carried out with homemade CE apparatus under the following operating conditior conditions: a fused-silica capillary col- umn of 50.0cm effect length and of 75m i.d. was used. 7 organic acids were used as BGAE, and a positive potential of separation in CZE with indirect detection. After optimizing for l3 native amino acids were established. Conclusion The choice of BGAe is an important factor influencing the efficiency of separation in CZE with indiect detection .After optimizing the separation conditions a baseline separation for 13 native amino acids is obtained.
基金Supported by the National Natural Science Foundation of China(Nos.30972263,30771644)the Natural Science Foundation of HunanProvince(No.09jj6037)
文摘The myosin heavy chain(MyHC)is one of the major structural and contracting proteins of muscle.We have isolated the cDNA clone encoding MyHC of the grass carp,Ctenopharyngodon idella. The sequence comprises 5 934 bp,including a 5 814 bp open reading frame encoding an amino acid sequence of 1 937 residues.The deduced amino acid sequence showed 69%homology to rabbit fast skeletal MyHC and 73%–76%homology to the MyHCs from the mandarin fish,walleye pollack,white croaker,chum salmon,and carp.The putative sequences of subfragment-1 and the light meromyosin region showed 61.4%–80%homology to the corresponding regions of other fish MyHCs.The tissue-specific and developmental stage-specific expressions of the MyHC gene were analyzed by quantitative real-time PCR.The MyHC gene showed the highest expression in the muscles compared with the kidney,spleen and intestine.Developmentally,there was a gradual increase in MyHC mRNA expression from the neural formation stage to the tail bud stage.The highest expression was detected in hatching larva.Our work on the MyHC gene from the grass carp has provided useful information for fish molecular biology and fish genomics.
基金V. ACKNOWLEDGMENTS This work was supported by tile National Natural Science Foundation of China （No.20721004 and No.20833008）. Can-hua Zhou sincerely wishes to express thanks to Dr. Ju-long Sun for assistance in the experiments.
文摘Photodissociation of p-aminobenzoic acid at 266 nm was investigated by probing the nascent OH photoproduct employing the laser-induced fluorescence technique. It was found that the nascent OH radical was vibrationally cold and its rotational state distribution conformed to be a Boltzmann behavior, characterized by a rotational temperature of 1040±110 K. The rotational energy of OH was determined to be 8.78±0.84 kJ/mol. Between the two spinorbit states of OH, ^2Ⅱ3/2 and ^2Ⅱ1/2, the former was found to be preferentially populated. The distribution of the II（A＇） state for the A-doublet was dominant. Finally, a probable mechanism for the formation of OH produced from the photodissociation of p-aminobenzoic acid is discussed.
