AIM： To observe the therapeutic effect of early administration of exogenous Basic fibroblast growth factor （bFGF） on acute edematous pancreatitis （AEP） in rats. METHODS： Thirty male Sprague-Dawley rats were rand...AIM： To observe the therapeutic effect of early administration of exogenous Basic fibroblast growth factor （bFGF） on acute edematous pancreatitis （AEP） in rats. METHODS： Thirty male Sprague-Dawley rats were randomly divided into three （n = 10）： normal control group （group Ⅰ）, AEP group （group Ⅱ） and AEP with bFGF treatment group （group Ⅲ）. AEP was induced by subcutaneous injection of cerulein （5.5 μg/kg and 7.5 μg/kg） at 1 h interval into rats of groups Ⅱ and Ⅲ. Three hours after induction of AEP, 100 μg/kg bFGF was administrated intraperitoneally for 1h to group Ⅲ rats. For test of DNA synthesis in acinar cells, 5-bromo-2＇-deoxyuridine （BrdU） labeling solution was intraperitoneally injected into the rats of groups Ⅱ and Ⅲ 24 h after bFGF treatment. The changes in serum amylase, lipase, pancreatic tissue wet/dry ratio were detected. RESULTS： In bFGF treatment group, there was a significant decrease in the volume of serum amylase, lipase and the pancreatic wet/dry weight ratio（1383.0 ± 94.6 U/L, 194.0 ± 43.6 U/L, 4.32 ± 0.32） compared to AEP group （3464 ± 223,7 U/L, 456 ±68,7 U/L, 6.89 ± 0,47） （P 〈 0.01）, and no significant difference was found between bFGF treatment and control group （1289 ± 94.0 U/L, 171 ± 23.4 U/L, 4.12 ± 0.26, P 〉 0.05）. The inflammatory changes such as interstitial edema, polymorphonuclear neutrophUs （PMNs） and vacuolization were significantly ameliorated compared to AEP group （P 〈 0.01）. A small number of BrdU-labeled nuclei were observed in acinar cells of AEP rats （1.8 ± 0.3 nuclei/microscopic field, n = 10） while diffuse BrdU-labeled nuclei were found in bFGF-treated rats （18.9 ± 1.4 nuclei/microscopic field, n = 10） （P 〈 0.01）. Immunohistochemical study showed increased DNA synthesis in pancreatic acinar cells. CONCLUSION： Early administration of exogenous bFGF has significant therapeutic effect on cerulein-induced acute edematous pancreatitis in rats. Its mechanism is related to the amelioration of inflammation and facilitation of pancreatic regeneration.展开更多
文摘AIM： To observe the therapeutic effect of early administration of exogenous Basic fibroblast growth factor （bFGF） on acute edematous pancreatitis （AEP） in rats. METHODS： Thirty male Sprague-Dawley rats were randomly divided into three （n = 10）： normal control group （group Ⅰ）, AEP group （group Ⅱ） and AEP with bFGF treatment group （group Ⅲ）. AEP was induced by subcutaneous injection of cerulein （5.5 μg/kg and 7.5 μg/kg） at 1 h interval into rats of groups Ⅱ and Ⅲ. Three hours after induction of AEP, 100 μg/kg bFGF was administrated intraperitoneally for 1h to group Ⅲ rats. For test of DNA synthesis in acinar cells, 5-bromo-2＇-deoxyuridine （BrdU） labeling solution was intraperitoneally injected into the rats of groups Ⅱ and Ⅲ 24 h after bFGF treatment. The changes in serum amylase, lipase, pancreatic tissue wet/dry ratio were detected. RESULTS： In bFGF treatment group, there was a significant decrease in the volume of serum amylase, lipase and the pancreatic wet/dry weight ratio（1383.0 ± 94.6 U/L, 194.0 ± 43.6 U/L, 4.32 ± 0.32） compared to AEP group （3464 ± 223,7 U/L, 456 ±68,7 U/L, 6.89 ± 0,47） （P 〈 0.01）, and no significant difference was found between bFGF treatment and control group （1289 ± 94.0 U/L, 171 ± 23.4 U/L, 4.12 ± 0.26, P 〉 0.05）. The inflammatory changes such as interstitial edema, polymorphonuclear neutrophUs （PMNs） and vacuolization were significantly ameliorated compared to AEP group （P 〈 0.01）. A small number of BrdU-labeled nuclei were observed in acinar cells of AEP rats （1.8 ± 0.3 nuclei/microscopic field, n = 10） while diffuse BrdU-labeled nuclei were found in bFGF-treated rats （18.9 ± 1.4 nuclei/microscopic field, n = 10） （P 〈 0.01）. Immunohistochemical study showed increased DNA synthesis in pancreatic acinar cells. CONCLUSION： Early administration of exogenous bFGF has significant therapeutic effect on cerulein-induced acute edematous pancreatitis in rats. Its mechanism is related to the amelioration of inflammation and facilitation of pancreatic regeneration.
