Protoplasts of the pathogenic plant fungus,Sclerotinia sclerotiorum,were transformed using the pPGF plasmid,which contains green fluorescent protein gene,under the control of Aspergillus nidulans regulatory sequences....Protoplasts of the pathogenic plant fungus,Sclerotinia sclerotiorum,were transformed using the pPGF plasmid,which contains green fluorescent protein gene,under the control of Aspergillus nidulans regulatory sequences. The pPGF plasmid was introduced by PEG/CaCl2 treatment. Positive transformants were harvested with hygromycin B (HYG) resistance as selective marker,and then were observed with green fluorescence phenomena in response to blue light,which suggested that GFP gene was cloned into genome DNA of S. sclerotiorum. The transformants were verified mitotically stable by Southern blotting analysis and passage culturing. This study is developed as an initial step for further research into infection mechanisms of S. sclerotiorum to plants and interactions with bio-control fungus.展开更多
With the depletion of fossil resources, there is a need to find alternative resources of fuels and chemicals. The use of renewable feedstock such as those from seed oil processing is one of the best available resource...With the depletion of fossil resources, there is a need to find alternative resources of fuels and chemicals. The use of renewable feedstock such as those from seed oil processing is one of the best available resources that have come to the fore-front recently. This paper critically analyzes and highlights major factors in the biodiesel industry, such as seeds oil composition, production methods, properties of biodiesel, problems and potential solutions of using vegetable seed oil, the composition, quality and effective utilization of crude glycerol, the catalytic conversion of glycerol into possible fuels and chemicals.展开更多
文摘Protoplasts of the pathogenic plant fungus,Sclerotinia sclerotiorum,were transformed using the pPGF plasmid,which contains green fluorescent protein gene,under the control of Aspergillus nidulans regulatory sequences. The pPGF plasmid was introduced by PEG/CaCl2 treatment. Positive transformants were harvested with hygromycin B (HYG) resistance as selective marker,and then were observed with green fluorescence phenomena in response to blue light,which suggested that GFP gene was cloned into genome DNA of S. sclerotiorum. The transformants were verified mitotically stable by Southern blotting analysis and passage culturing. This study is developed as an initial step for further research into infection mechanisms of S. sclerotiorum to plants and interactions with bio-control fungus.
基金supported by the National Natural Science Foundation of China(21325208,21172209,21272050)the Chinese Academy of Sciences(KJCX2-EW-J02)FRFCU(WK2060190025,WK2060190033)
文摘With the depletion of fossil resources, there is a need to find alternative resources of fuels and chemicals. The use of renewable feedstock such as those from seed oil processing is one of the best available resources that have come to the fore-front recently. This paper critically analyzes and highlights major factors in the biodiesel industry, such as seeds oil composition, production methods, properties of biodiesel, problems and potential solutions of using vegetable seed oil, the composition, quality and effective utilization of crude glycerol, the catalytic conversion of glycerol into possible fuels and chemicals.
