通过培养基优化、核糖体工程对链霉菌(Streptomyces sp.)FJS31-2外分泌物产生条件进行了优化,利用高分辨质谱(HRMS)技术对其主要活性产物的成分进行了初步分析,采用磺酰罗丹明B(SRB)比色法测定了外分泌物对肝癌细胞MHCC97H的抑制活性。...通过培养基优化、核糖体工程对链霉菌(Streptomyces sp.)FJS31-2外分泌物产生条件进行了优化,利用高分辨质谱(HRMS)技术对其主要活性产物的成分进行了初步分析,采用磺酰罗丹明B(SRB)比色法测定了外分泌物对肝癌细胞MHCC97H的抑制活性。结果表明,经链霉素多代胁迫结合培养基优化诱导链霉菌FJS31-2产生了分子式为C 28 H 40 O 2的四环萜类化合物,该化合物可抑制肝癌细胞MHCC97H的生长,并呈浓度依赖性。为从链霉菌FJS31-2获取新型抗生素奠定了技术和物质基础。展开更多
A pair of primers containing BamHI and XhoI sites was designed to amplify n gene of 1149bp from the positive pMD18-TN plasmid of transmissible gastroenteritis virus n gene by PCR.PCR product was subcloned into multipl...A pair of primers containing BamHI and XhoI sites was designed to amplify n gene of 1149bp from the positive pMD18-TN plasmid of transmissible gastroenteritis virus n gene by PCR.PCR product was subcloned into multiple cloning sites of pET-30a containing 6 His·Tag.Recombinant plasmid pET-30a of n gene named pET30a-N was transfected into E.coli BL21 and the bacteria was induced with IPTG at 37℃.It was demonstrated by SDS-PAGE that recombinant N protein expressed in E.coli BL21 was soluble protein and its molecular weight was about 52kD.The result of Western blot test showed that immunoactivity of the recombinant N protein was the same to that of the natural N protein.展开更多
A variety of technologies continue to develop for high throughput screening This review summarized recent advances of bioassay techniques used in high throughput screening The principles, applications, characteristics...A variety of technologies continue to develop for high throughput screening This review summarized recent advances of bioassay techniques used in high throughput screening The principles, applications, characteristics and other parameters of these methods were described such as homogeneous time resolved fluorescence, fluorescence polarization, fluorescence resonance energy transfer, fluorescence correlation spectroscopy and scintillation proximity展开更多
文摘通过培养基优化、核糖体工程对链霉菌(Streptomyces sp.)FJS31-2外分泌物产生条件进行了优化,利用高分辨质谱(HRMS)技术对其主要活性产物的成分进行了初步分析,采用磺酰罗丹明B(SRB)比色法测定了外分泌物对肝癌细胞MHCC97H的抑制活性。结果表明,经链霉素多代胁迫结合培养基优化诱导链霉菌FJS31-2产生了分子式为C 28 H 40 O 2的四环萜类化合物,该化合物可抑制肝癌细胞MHCC97H的生长,并呈浓度依赖性。为从链霉菌FJS31-2获取新型抗生素奠定了技术和物质基础。
文摘A pair of primers containing BamHI and XhoI sites was designed to amplify n gene of 1149bp from the positive pMD18-TN plasmid of transmissible gastroenteritis virus n gene by PCR.PCR product was subcloned into multiple cloning sites of pET-30a containing 6 His·Tag.Recombinant plasmid pET-30a of n gene named pET30a-N was transfected into E.coli BL21 and the bacteria was induced with IPTG at 37℃.It was demonstrated by SDS-PAGE that recombinant N protein expressed in E.coli BL21 was soluble protein and its molecular weight was about 52kD.The result of Western blot test showed that immunoactivity of the recombinant N protein was the same to that of the natural N protein.
文摘A variety of technologies continue to develop for high throughput screening This review summarized recent advances of bioassay techniques used in high throughput screening The principles, applications, characteristics and other parameters of these methods were described such as homogeneous time resolved fluorescence, fluorescence polarization, fluorescence resonance energy transfer, fluorescence correlation spectroscopy and scintillation proximity
