早产儿呼吸窘迫综合征在进行肺表面活性物质替代治疗时,传统上需要机械通气,通过气管插管给药。但众所周知,机械通气和气管插管操作容易导致多种并发症的发生,如局部损伤出血、气胸、感染和支气管肺发育不良等。为了充分发挥肺表面活性...早产儿呼吸窘迫综合征在进行肺表面活性物质替代治疗时,传统上需要机械通气,通过气管插管给药。但众所周知,机械通气和气管插管操作容易导致多种并发症的发生,如局部损伤出血、气胸、感染和支气管肺发育不良等。为了充分发挥肺表面活性物质的效果,减少甚至避免机械通气和气管插管的不良影响,微创肺表面活性物质给药技术日益创新。这些技术包括气管插管–使用肺表面活性物质–拔管(INSURE)、咽部给药、喉罩给药、雾化吸入给药和侵入性较小的肺表面活性物质给药(LISA)等。其中,LISA已经得到了最广泛的研究,并有望作为一种对早产儿进行肺表面活性物质给药侵入性较小且有效的方式。现就微创肺表面活性物质给药技术研究进展作一综述。Traditionally, surfactant has been administered to preterm infants with respiratory distress syndrome by endotracheal tube after intubation and mechanical ventilation. However, it is well known that mechanical ventilation and endotracheal intubation can easily lead to a variety of complications, such as local injury bleeding, pneumothorax, infection, and bronchopulmonary dysplasia. In order to provide the benefits of surfactant and minimize the trauma caused by intubation and mechanical ventilation, several surfactant administration methods of less invasive have been developed. These techniques include INSURE (intubate-surfactant-extubate), pharyngeal administration, laryngeal mask administration, aerosolized surfactant administration, and less invasive surfactant administration (LISA). Of these, LISA has been studied extensively and holds the most promise as a less invasive and effective technique of surfactant administration to preterm infants. This article reviewed administration methods of minimally invasive surfactant technology in preterm infants with respiratory distress syndrome.展开更多
建立了污水中26种痕量卡西酮类新精神活性物质的前处理及同时定量检测的方法,并成功应用于实际污水样品的检测。污水样品经过滤和加入内标溶液的预处理,在Oasis PRiME HLB固相萃取柱上经超纯水淋洗和甲醇溶液洗脱,在40℃下氮气吹干,用0...建立了污水中26种痕量卡西酮类新精神活性物质的前处理及同时定量检测的方法,并成功应用于实际污水样品的检测。污水样品经过滤和加入内标溶液的预处理,在Oasis PRiME HLB固相萃取柱上经超纯水淋洗和甲醇溶液洗脱,在40℃下氮气吹干,用0.1%甲酸-乙腈溶液(95∶5)复溶,利用高效液相色谱-串联质谱(high-performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)技术,采用选择反应监测(selected reaction monitoring,SRM)模式,用UPLC BEH C_(18)(100 mm×2.1 mm,1.7μm)色谱柱,柱温35℃,流动相为乙腈-0.1%甲酸的水溶液,梯度洗脱进行检测。经方法学验证,26种卡西酮类新精神活性物质的定量下限可达1.50~3.00 ng/L,其中21种待测物在1.50~375.0 ng/L范围内,5种在3.00~750.0 ng/L范围内,所有26种物质均具有良好的线性关系,相关系数r为0.99,批内和批间精密度的相对标准偏差(relative standard deviation,RSD)分别小于7.71%和13.91%,提取回收率均高于92.64%。该方法简单、准确、灵敏,可作为卡西酮类物质检测和滥用情况监测的方法。展开更多
为促进东北地区浆果相关产品的开发与利用,以东北9种种植范围广且常见的浆果为研究对象,测定分析浆果中总酚、总黄酮和总花色苷含量,通过高效液相色谱法测定浆果中酚类单体的种类和含量;通过测定DPPH自由基清除能力、ABTS~+自由基清除...为促进东北地区浆果相关产品的开发与利用,以东北9种种植范围广且常见的浆果为研究对象,测定分析浆果中总酚、总黄酮和总花色苷含量,通过高效液相色谱法测定浆果中酚类单体的种类和含量;通过测定DPPH自由基清除能力、ABTS~+自由基清除能力、透明质酸酶抑制能力和抑制白蛋白变性能力评价浆果抗氧化活性和抗炎活性,并进行相关性分析。结果显示,红豆越橘和蓝靛果中总酚、总黄酮和总花色苷含量较高,分别为2.83和2.42 g GAE/100 g DW、0.99和2.09 g Rutin/100 g DW、104.33和414.73 mg/100 g DW,且二者具有较强的抗氧化活性和抗炎活性。相关性结果表明,总酚含量决定浆果的抗氧化活性和抗炎活性。在9种浆果中鉴定并定量19种单体酚,绿原酸、隐绿原酸、熊果苷和黄岑素均在浆果中检出,绿原酸和隐绿原酸是大部分浆果中主要的酚类单体,红豆越橘中单体酚种类最多,蓝靛果中单体酚含量最高。该实验为东北浆果的综合利用提供理论依据。展开更多
文摘早产儿呼吸窘迫综合征在进行肺表面活性物质替代治疗时,传统上需要机械通气,通过气管插管给药。但众所周知,机械通气和气管插管操作容易导致多种并发症的发生,如局部损伤出血、气胸、感染和支气管肺发育不良等。为了充分发挥肺表面活性物质的效果,减少甚至避免机械通气和气管插管的不良影响,微创肺表面活性物质给药技术日益创新。这些技术包括气管插管–使用肺表面活性物质–拔管(INSURE)、咽部给药、喉罩给药、雾化吸入给药和侵入性较小的肺表面活性物质给药(LISA)等。其中,LISA已经得到了最广泛的研究,并有望作为一种对早产儿进行肺表面活性物质给药侵入性较小且有效的方式。现就微创肺表面活性物质给药技术研究进展作一综述。Traditionally, surfactant has been administered to preterm infants with respiratory distress syndrome by endotracheal tube after intubation and mechanical ventilation. However, it is well known that mechanical ventilation and endotracheal intubation can easily lead to a variety of complications, such as local injury bleeding, pneumothorax, infection, and bronchopulmonary dysplasia. In order to provide the benefits of surfactant and minimize the trauma caused by intubation and mechanical ventilation, several surfactant administration methods of less invasive have been developed. These techniques include INSURE (intubate-surfactant-extubate), pharyngeal administration, laryngeal mask administration, aerosolized surfactant administration, and less invasive surfactant administration (LISA). Of these, LISA has been studied extensively and holds the most promise as a less invasive and effective technique of surfactant administration to preterm infants. This article reviewed administration methods of minimally invasive surfactant technology in preterm infants with respiratory distress syndrome.
文摘为促进东北地区浆果相关产品的开发与利用,以东北9种种植范围广且常见的浆果为研究对象,测定分析浆果中总酚、总黄酮和总花色苷含量,通过高效液相色谱法测定浆果中酚类单体的种类和含量;通过测定DPPH自由基清除能力、ABTS~+自由基清除能力、透明质酸酶抑制能力和抑制白蛋白变性能力评价浆果抗氧化活性和抗炎活性,并进行相关性分析。结果显示,红豆越橘和蓝靛果中总酚、总黄酮和总花色苷含量较高,分别为2.83和2.42 g GAE/100 g DW、0.99和2.09 g Rutin/100 g DW、104.33和414.73 mg/100 g DW,且二者具有较强的抗氧化活性和抗炎活性。相关性结果表明,总酚含量决定浆果的抗氧化活性和抗炎活性。在9种浆果中鉴定并定量19种单体酚,绿原酸、隐绿原酸、熊果苷和黄岑素均在浆果中检出,绿原酸和隐绿原酸是大部分浆果中主要的酚类单体,红豆越橘中单体酚种类最多,蓝靛果中单体酚含量最高。该实验为东北浆果的综合利用提供理论依据。
