Mass microalgal culture plays an irreplaceable role in aquaculture,but microalgal productivity is restricted by traditional autotrophic culture conditions.In the present study,a Tetraselmis chuii strain belonging to t...Mass microalgal culture plays an irreplaceable role in aquaculture,but microalgal productivity is restricted by traditional autotrophic culture conditions.In the present study,a Tetraselmis chuii strain belonging to the phylum Chlorophyta was isolated from south Yellow Sea.The growth rate and biomass productivity of this strain was higher under mixotrophic conditions with different carbon:nitrogen(C:N)ratios than those under autotrophic conditions.When the C:N ratio was 16,the optical density and biomass productivity were 3.7-and 5-fold higher than their corresponding values under autotrophic culture conditions,respectively.Moreover,T.chuii synthesized more polysaccharides and total lipids under mixotrophic conditions.In addition,T.chuii cultured under mixotrophic conditions synthesized more types of fatty acids than autotrophic culture conditions.At a C:N ratio of 16,the percentage of C16:0 and C18:1 reached 30.08%and 24.65%of the total fatty acid(TFA) content,respectively.These findings may provide a basis for largescale mixotrophic culture of T.chuii,as a potential bait-microalga.展开更多
Tetraselmis sp.-1 is a new microalgae strain constructed by cell fusion technique. In this paper, the mixotrophic cultivation of Tetraselmis sp.-1 in airlift photobioreactor is investigated. Firstly, the paper calcula...Tetraselmis sp.-1 is a new microalgae strain constructed by cell fusion technique. In this paper, the mixotrophic cultivation of Tetraselmis sp.-1 in airlift photobioreactor is investigated. Firstly, the paper calculates the light attenuation in the mixotrophic medium, and sets the light attenuation model. Secondly, it uses the same dissolved oxygen coefficient (K d) of flask culture to select the aeration of bioreactor. Finally, it sets the growth kinetic model, production (chlorophyll-a and total lipid) kinetic models and substrate (glucose) consumption kinetic model of Tetraselmis sp.-1 in airlift photobioreactor.展开更多
基金Supported by the National Basic Research Program of China(973Program)(Nos.2011CB200901,2011CB200904)the National Key Technology Research and Development Program(No.2011BAD14B01)+1 种基金the National High Technology Research and Development Program of China(863 Program)(No.2013AA065801)the Special Foundation for Marine Renewable Energy(No.GHME2011SW03)
文摘Mass microalgal culture plays an irreplaceable role in aquaculture,but microalgal productivity is restricted by traditional autotrophic culture conditions.In the present study,a Tetraselmis chuii strain belonging to the phylum Chlorophyta was isolated from south Yellow Sea.The growth rate and biomass productivity of this strain was higher under mixotrophic conditions with different carbon:nitrogen(C:N)ratios than those under autotrophic conditions.When the C:N ratio was 16,the optical density and biomass productivity were 3.7-and 5-fold higher than their corresponding values under autotrophic culture conditions,respectively.Moreover,T.chuii synthesized more polysaccharides and total lipids under mixotrophic conditions.In addition,T.chuii cultured under mixotrophic conditions synthesized more types of fatty acids than autotrophic culture conditions.At a C:N ratio of 16,the percentage of C16:0 and C18:1 reached 30.08%and 24.65%of the total fatty acid(TFA) content,respectively.These findings may provide a basis for largescale mixotrophic culture of T.chuii,as a potential bait-microalga.
文摘Tetraselmis sp.-1 is a new microalgae strain constructed by cell fusion technique. In this paper, the mixotrophic cultivation of Tetraselmis sp.-1 in airlift photobioreactor is investigated. Firstly, the paper calculates the light attenuation in the mixotrophic medium, and sets the light attenuation model. Secondly, it uses the same dissolved oxygen coefficient (K d) of flask culture to select the aeration of bioreactor. Finally, it sets the growth kinetic model, production (chlorophyll-a and total lipid) kinetic models and substrate (glucose) consumption kinetic model of Tetraselmis sp.-1 in airlift photobioreactor.
