Objective To explore the effect of moxibustion at Shenque （神阙 CV 8） on the anti-exercise-induced fatigue in rats after exhaustive exercise in varying degrees. Methods Seventy-two SD rats were randomly divided int...Objective To explore the effect of moxibustion at Shenque （神阙 CV 8） on the anti-exercise-induced fatigue in rats after exhaustive exercise in varying degrees. Methods Seventy-two SD rats were randomly divided into 3 groups according to the random number table method, including blank group, model group and moxibustion group. The model group and moxibustion group were divided into 4 subgroups respectively, that was 1 time, 4 times, 7 times and 10 times subgroup. There were 9 groups with 8 rats in each group. Except blank group, all the rats were established into exhaustive models through swimming. Mild moxibustion at CV 8 was carried out for 15 min in the rats in moxibustion group after modeling for once every other day. The concentration of serum glucose （GLU）, malonaldehyde （MDA） and blood urea nitrogen （BUN） as well as the activity of aspartate transaminase （AST）, alanine transaminase （ALT） and lactic dehydrogenase （LDH） were detected 24h after the exhaustive exercise by adopting full-automatic analyzer. Results The exhaustive time of rats were long at first but shorter later. The exhaustive time of rats in the model group （4 times） were obviously longer than that in the model group （1 time） （P〈0.01）, the exhaustive time of rats in the model group （7 times） and model group （10 times） were obviously shorter than that in the model group （1 time） （P〈0.01）, and the exhaustive time of rats in the moxibustion group （7 times） and moxibustion group （10 times） were obviously longer than that in the model group with the same number of times （P〈0.01）, the serum GLU level of rats in the model group （7 times） and model group （10 times） increased when compared with the blank group （P〈0.01）, and the serum MDA and BNU levels as well as the activity of AST, ALT and LDH of rats in the model group （4 times）, model group （7 times） and model group （10 times） increased when compared with the blank group （P〈0.05 or P〈0.01）, the serum GLU, MDA and BNU levels as well as the activity of AST and ALT of rats in the moxibustion group （7 times） and moxibustion group （10 times） reduced when compared with the model group with the same number of times （P〈0.05 or P〈0.01）, and the serum LDH activity of rats in the moxibustion group （4 times）, moxibustion group （7 times） and moxibustion group （10 times） reduced when compared with the model group with the same number of times （P〈0.05 or P〈0.01）. Conclusion Moxibustion at CV 8 can reduce the serum MDA and BNU levels as well as the activity of AST, ALT and LDH of organism in a status of long-term fatigue, effectively improve the symptom of long-term fatigue, providing a certain experimental basis for the clinical application of moxibustion at CV 8 in the process of anti-exercise-induced fatigue in the future.展开更多
Objective:To investigate the effect of moxibustion at Shenque(CV 8)on fatigue in rats with chronic exercise-induced exhaustion.Methods:Thirty male Sprague-Dawley(SD)rats were randomly divided into a blank group,a mode...Objective:To investigate the effect of moxibustion at Shenque(CV 8)on fatigue in rats with chronic exercise-induced exhaustion.Methods:Thirty male Sprague-Dawley(SD)rats were randomly divided into a blank group,a model group and a moxibustion group,10 rats in each group.Except rats in the blank group,the remaining rats were subjected to create long-term exhaustion models by repeated swimming.After successful modeling,rats in the moxibustion group received mild moxibustion at Shenque(CV 8)for 15 min,once every other day with a total of 10 times.Rats in the model group and the blank group did not receive moxibustion.At the end of the treatment,the exhausted times,and the body weight of rats before and after the experiment were compared among groups.The levels of blood malondialdehyde(MDA)and urea nitrogen(BUN),as well as the activities of aspartate transarninase(AST),alanine aminotransferase(ALT)and lactate dehydrogenase(LDH)were also measured by the automatic biochemical analyzer,24 h after the exhausting excise.Results:The 10 th swimming time was significantly longer in the moxibustion group than that in the model group(P〈0.01).The increase rate of the body weight was lower in the rats of the moxibustion group than that in the model group before the 7 th and the 10 th exhausting excise(P〈0.05,P〈0.01).The levels of serum MDA and BUN,as well as the activities of AST,ALT and LDH in the model group were higher than those in the blank group(all P〈0.01).The levels of serum MDA and BUN,as well as the activities of AST,ALT and LDH in the moxibustion group were lower than those in the model group(P〈0.01).Conclusion:Moxibustion at Shenque(CV 8)can decrease the serum levels of MDA and BUN,as well as activities of AST,ALT and LDH in the long-term fatigue rats,thus to improve the symptoms of fatigue.展开更多
Objective： To investigate the effect of moxibustion at Shenque （CV 8） on free radical metabolism in rat＇s hippocampus after different degrees of exhaustive exercise. Methods： A total of 72 male Sprague-Dawley （S...Objective： To investigate the effect of moxibustion at Shenque （CV 8） on free radical metabolism in rat＇s hippocampus after different degrees of exhaustive exercise. Methods： A total of 72 male Sprague-Dawley （SD） rats were randomly divided into a normal group （n=8）, a model group （n=32） and a moxibustion group （n=32）. According to the times of modeling or treatment, the model group was further randomly divided into different subgroups of a 1-time model subgroup, a 4-time model subgroup, a 7-time model subgroup and a 10-time model subgroup （n=8）; the moxibustion group was also further randomly divided into different subgroups of a 1-time moxibustion subgroup, a 4-time moxibustion subgroup, a 7-time moxibustion subgroup and a 10-time moxibustion subgroup （n=8）. Rats in both model and moxibustion subgroups were subjected to establishing the swimming exhaustive model. Rats in each moxibustion subgroup received mild moxibustion at Shenque （CV 8） for 15 min immediately after modeling, once every other day. The concentration of malic dialdehyde （MDA）, as well as the activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and total antioxidant capacity （T-AOC） in rat＇s hippocampus in each group were detected 24 h after the exhaustive exercise. Results： Compared with the 1-time model subgroup, the exhaustive swimming time of rats was significantly prolonged in the 4-time model subgroup （P〈0.01）, while it was significantly shortened in the 7-time and 10-time model subgroups （both P〈0.01）. Compared with the matched model subgroup, the exhaustive swimming time of rats in the 7-time and 10-time moxibustion subgroups was significantly prolonged （both P〈0.01）. Compared with the normal group, the MDA concentration was increased significantly （P〈0.01）, and the activities stress response of SOD and T-AOC were increased in the 1-time model subgroup （both P〈0.05）; the MDA concentration was increased （all P〈0.01）, and the activities of SOD, GSH-Px and T-AOC were decreased differently （P〈0.05 or P〈0.01） in the 4-time, 7-time and 10-time model subgroups. Compared with the matched model subgroup, the concentration of MDA was significantly reduced （P〈0.05 or P〈0.01）, and the activities of SOD, GSH-Px and T-AOC were significantly increased in the 4-time, 7-time and 10-time moxibustion subgroups （all P〈0.01）. Conclusion： Moxibustion at Shenque （CV 8） can improve the fatigue status of the body after long-term exhaustive exercise by regulating free radical metabolism in rat＇s hippocampus. To some extent, this provides an experimental basis for moxibustion at Shenque （CV 8） against exercise-induced fatigue.展开更多
基金Supported by Natural Science Fund Project of Youth of Hebei Education Department:QN2016021Key Scientific Project of Administration of Traditional Chinese Medicine of Hebei Province:2016011 and 2017011
文摘Objective To explore the effect of moxibustion at Shenque （神阙 CV 8） on the anti-exercise-induced fatigue in rats after exhaustive exercise in varying degrees. Methods Seventy-two SD rats were randomly divided into 3 groups according to the random number table method, including blank group, model group and moxibustion group. The model group and moxibustion group were divided into 4 subgroups respectively, that was 1 time, 4 times, 7 times and 10 times subgroup. There were 9 groups with 8 rats in each group. Except blank group, all the rats were established into exhaustive models through swimming. Mild moxibustion at CV 8 was carried out for 15 min in the rats in moxibustion group after modeling for once every other day. The concentration of serum glucose （GLU）, malonaldehyde （MDA） and blood urea nitrogen （BUN） as well as the activity of aspartate transaminase （AST）, alanine transaminase （ALT） and lactic dehydrogenase （LDH） were detected 24h after the exhaustive exercise by adopting full-automatic analyzer. Results The exhaustive time of rats were long at first but shorter later. The exhaustive time of rats in the model group （4 times） were obviously longer than that in the model group （1 time） （P〈0.01）, the exhaustive time of rats in the model group （7 times） and model group （10 times） were obviously shorter than that in the model group （1 time） （P〈0.01）, and the exhaustive time of rats in the moxibustion group （7 times） and moxibustion group （10 times） were obviously longer than that in the model group with the same number of times （P〈0.01）, the serum GLU level of rats in the model group （7 times） and model group （10 times） increased when compared with the blank group （P〈0.01）, and the serum MDA and BNU levels as well as the activity of AST, ALT and LDH of rats in the model group （4 times）, model group （7 times） and model group （10 times） increased when compared with the blank group （P〈0.05 or P〈0.01）, the serum GLU, MDA and BNU levels as well as the activity of AST and ALT of rats in the moxibustion group （7 times） and moxibustion group （10 times） reduced when compared with the model group with the same number of times （P〈0.05 or P〈0.01）, and the serum LDH activity of rats in the moxibustion group （4 times）, moxibustion group （7 times） and moxibustion group （10 times） reduced when compared with the model group with the same number of times （P〈0.05 or P〈0.01）. Conclusion Moxibustion at CV 8 can reduce the serum MDA and BNU levels as well as the activity of AST, ALT and LDH of organism in a status of long-term fatigue, effectively improve the symptom of long-term fatigue, providing a certain experimental basis for the clinical application of moxibustion at CV 8 in the process of anti-exercise-induced fatigue in the future.
文摘Objective:To investigate the effect of moxibustion at Shenque(CV 8)on fatigue in rats with chronic exercise-induced exhaustion.Methods:Thirty male Sprague-Dawley(SD)rats were randomly divided into a blank group,a model group and a moxibustion group,10 rats in each group.Except rats in the blank group,the remaining rats were subjected to create long-term exhaustion models by repeated swimming.After successful modeling,rats in the moxibustion group received mild moxibustion at Shenque(CV 8)for 15 min,once every other day with a total of 10 times.Rats in the model group and the blank group did not receive moxibustion.At the end of the treatment,the exhausted times,and the body weight of rats before and after the experiment were compared among groups.The levels of blood malondialdehyde(MDA)and urea nitrogen(BUN),as well as the activities of aspartate transarninase(AST),alanine aminotransferase(ALT)and lactate dehydrogenase(LDH)were also measured by the automatic biochemical analyzer,24 h after the exhausting excise.Results:The 10 th swimming time was significantly longer in the moxibustion group than that in the model group(P〈0.01).The increase rate of the body weight was lower in the rats of the moxibustion group than that in the model group before the 7 th and the 10 th exhausting excise(P〈0.05,P〈0.01).The levels of serum MDA and BUN,as well as the activities of AST,ALT and LDH in the model group were higher than those in the blank group(all P〈0.01).The levels of serum MDA and BUN,as well as the activities of AST,ALT and LDH in the moxibustion group were lower than those in the model group(P〈0.01).Conclusion:Moxibustion at Shenque(CV 8)can decrease the serum levels of MDA and BUN,as well as activities of AST,ALT and LDH in the long-term fatigue rats,thus to improve the symptoms of fatigue.
文摘Objective： To investigate the effect of moxibustion at Shenque （CV 8） on free radical metabolism in rat＇s hippocampus after different degrees of exhaustive exercise. Methods： A total of 72 male Sprague-Dawley （SD） rats were randomly divided into a normal group （n=8）, a model group （n=32） and a moxibustion group （n=32）. According to the times of modeling or treatment, the model group was further randomly divided into different subgroups of a 1-time model subgroup, a 4-time model subgroup, a 7-time model subgroup and a 10-time model subgroup （n=8）; the moxibustion group was also further randomly divided into different subgroups of a 1-time moxibustion subgroup, a 4-time moxibustion subgroup, a 7-time moxibustion subgroup and a 10-time moxibustion subgroup （n=8）. Rats in both model and moxibustion subgroups were subjected to establishing the swimming exhaustive model. Rats in each moxibustion subgroup received mild moxibustion at Shenque （CV 8） for 15 min immediately after modeling, once every other day. The concentration of malic dialdehyde （MDA）, as well as the activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and total antioxidant capacity （T-AOC） in rat＇s hippocampus in each group were detected 24 h after the exhaustive exercise. Results： Compared with the 1-time model subgroup, the exhaustive swimming time of rats was significantly prolonged in the 4-time model subgroup （P〈0.01）, while it was significantly shortened in the 7-time and 10-time model subgroups （both P〈0.01）. Compared with the matched model subgroup, the exhaustive swimming time of rats in the 7-time and 10-time moxibustion subgroups was significantly prolonged （both P〈0.01）. Compared with the normal group, the MDA concentration was increased significantly （P〈0.01）, and the activities stress response of SOD and T-AOC were increased in the 1-time model subgroup （both P〈0.05）; the MDA concentration was increased （all P〈0.01）, and the activities of SOD, GSH-Px and T-AOC were decreased differently （P〈0.05 or P〈0.01） in the 4-time, 7-time and 10-time model subgroups. Compared with the matched model subgroup, the concentration of MDA was significantly reduced （P〈0.05 or P〈0.01）, and the activities of SOD, GSH-Px and T-AOC were significantly increased in the 4-time, 7-time and 10-time moxibustion subgroups （all P〈0.01）. Conclusion： Moxibustion at Shenque （CV 8） can improve the fatigue status of the body after long-term exhaustive exercise by regulating free radical metabolism in rat＇s hippocampus. To some extent, this provides an experimental basis for moxibustion at Shenque （CV 8） against exercise-induced fatigue.
