【目的】组建铁心杉优良杂交组合,为铁心杉种质资源圃构建及种子园配置提供数据支撑和科学依据。【方法】选取铁心杉优良母树3576个半同胞子代中表现优良的幼苗优株以及403个候选父本、10个母本作为研究对象,筛选15个EST-SSR标记,采用AB...【目的】组建铁心杉优良杂交组合,为铁心杉种质资源圃构建及种子园配置提供数据支撑和科学依据。【方法】选取铁心杉优良母树3576个半同胞子代中表现优良的幼苗优株以及403个候选父本、10个母本作为研究对象,筛选15个EST-SSR标记,采用ABI 370XL毛细管电泳检测PCR扩增产物,利用软件GeneMarker 2.20进行基因分型、软件PIC_CALC Version 0.6计算多态信息含量(PIC),GenALex6.5估算各个SSR位点的等位基因数目(NA)、期望杂合度(HE)、观测杂合度(HO);以等位基因分布频率为基础,CERVUS3.0.7软件和最大似然值法计算LOD值进行父本鉴定,计算NE-1P、NE-2P和NE-PP。【结果】共计选出50株铁心杉半同胞优株;15个SSR位点在所有样本中的平均等位基因数、平均观测杂合度和平均期望杂合度分别为9.867、0.475和0.679;15个SSR位点的多态信息含量PIC值在0.356~0.876,平均为0.634;平均NE-1P、NE-2P和NE-PP为0.872、0.802、0.702;50个半同胞子代在403个疑似父本中检测出的最似父本LOD值均大于0,其中TXS-14的繁殖贡献率最高为6%,TXS-20、TXS-201、TXS-204、TXS-399、TXS-5的繁殖贡献率次之,为4%,其余均为2%,共鉴定出42个真实父本,选出高配合力亲本杂交组合50对。【结论】已发表的SSR标记能够用来对铁心杉半同胞子代优株进行父本鉴定,研究结果将为铁心杉种质资源圃的构建提供理论依据和进一步开展铁心杉分子辅助杂交育种奠定基础。展开更多
Fourteen 10 mer primers were used for RAPD (Random amplified polymorphic DNA) analysis across a fullsib family, including 2 parents and 18 sibs, of Liriodendron chinense×L. Tulipifera. Of the total 57 fragments a...Fourteen 10 mer primers were used for RAPD (Random amplified polymorphic DNA) analysis across a fullsib family, including 2 parents and 18 sibs, of Liriodendron chinense×L. Tulipifera. Of the total 57 fragments amplified, 20 (35 09%) were polymorphic between the 2 parents and 28 (49 12%) segregants in the sibs, indicting a little lower of polymorphism and heterozygosity at RAPD loci in the 2 species, compared with other tree species studied. Of the 20 polymorphic loci, 4 (appeared in the male parent) did not segregate in the progeny, indicating the genotype in these loci were AA ( α =0 5 18 ), so, may be used as paternal markers for paternal identification. Fourteen parental polymorphic loci (24 56%) segregated 1∶1 in the progeny as expected, and 12 parental monomorphic loci (21 05%) segregated 3∶1 as expected, showing a Mendelian dominant inheritance fashion for an allele. Two parental monomorphic loci (3 51%) were detected to segregate in aberration from the expected ratios (3∶1).展开更多
文摘【目的】组建铁心杉优良杂交组合,为铁心杉种质资源圃构建及种子园配置提供数据支撑和科学依据。【方法】选取铁心杉优良母树3576个半同胞子代中表现优良的幼苗优株以及403个候选父本、10个母本作为研究对象,筛选15个EST-SSR标记,采用ABI 370XL毛细管电泳检测PCR扩增产物,利用软件GeneMarker 2.20进行基因分型、软件PIC_CALC Version 0.6计算多态信息含量(PIC),GenALex6.5估算各个SSR位点的等位基因数目(NA)、期望杂合度(HE)、观测杂合度(HO);以等位基因分布频率为基础,CERVUS3.0.7软件和最大似然值法计算LOD值进行父本鉴定,计算NE-1P、NE-2P和NE-PP。【结果】共计选出50株铁心杉半同胞优株;15个SSR位点在所有样本中的平均等位基因数、平均观测杂合度和平均期望杂合度分别为9.867、0.475和0.679;15个SSR位点的多态信息含量PIC值在0.356~0.876,平均为0.634;平均NE-1P、NE-2P和NE-PP为0.872、0.802、0.702;50个半同胞子代在403个疑似父本中检测出的最似父本LOD值均大于0,其中TXS-14的繁殖贡献率最高为6%,TXS-20、TXS-201、TXS-204、TXS-399、TXS-5的繁殖贡献率次之,为4%,其余均为2%,共鉴定出42个真实父本,选出高配合力亲本杂交组合50对。【结论】已发表的SSR标记能够用来对铁心杉半同胞子代优株进行父本鉴定,研究结果将为铁心杉种质资源圃的构建提供理论依据和进一步开展铁心杉分子辅助杂交育种奠定基础。
文摘Fourteen 10 mer primers were used for RAPD (Random amplified polymorphic DNA) analysis across a fullsib family, including 2 parents and 18 sibs, of Liriodendron chinense×L. Tulipifera. Of the total 57 fragments amplified, 20 (35 09%) were polymorphic between the 2 parents and 28 (49 12%) segregants in the sibs, indicting a little lower of polymorphism and heterozygosity at RAPD loci in the 2 species, compared with other tree species studied. Of the 20 polymorphic loci, 4 (appeared in the male parent) did not segregate in the progeny, indicating the genotype in these loci were AA ( α =0 5 18 ), so, may be used as paternal markers for paternal identification. Fourteen parental polymorphic loci (24 56%) segregated 1∶1 in the progeny as expected, and 12 parental monomorphic loci (21 05%) segregated 3∶1 as expected, showing a Mendelian dominant inheritance fashion for an allele. Two parental monomorphic loci (3 51%) were detected to segregate in aberration from the expected ratios (3∶1).
