A井区位于鄂尔多斯盆地西部,是长庆油田的重要油气产区之一,马家沟组是该井区的主力油气储层,属低孔、低渗储层。论文通过常规物性实验、压汞实验和铸体薄片观察测定实验对所取的260余块岩心样品进行检测分析,得到了A井区马五段的孔渗...A井区位于鄂尔多斯盆地西部,是长庆油田的重要油气产区之一,马家沟组是该井区的主力油气储层,属低孔、低渗储层。论文通过常规物性实验、压汞实验和铸体薄片观察测定实验对所取的260余块岩心样品进行检测分析,得到了A井区马五段的孔渗值、孔隙特征和储层矿物成分以及孔隙类型情况。研究表明:马五段储层孔隙度平均值为3.78%,78.3%样品的渗透率小于0.5 m D,测得所选样品平均孔喉半径值为5.17μm,而且分选性差。马五段储层为碳酸盐岩,所含矿物类型有白云石、方解石、泥质、硅质、伊利石和黄铁矿等,镜下观察到的孔隙类型有溶缝、溶孔、溶洞、膏模孔及构造裂隙,常伴随不同矿物的结晶充填。展开更多
To develop a new method of inducing mdrl multidrug resistance by establishinga nude mice model of orthotopic transplantation of liver carcinoma by sporadic abdominalchemotherapy at intervals. Methods: Hepatocellular c...To develop a new method of inducing mdrl multidrug resistance by establishinga nude mice model of orthotopic transplantation of liver carcinoma by sporadic abdominalchemotherapy at intervals. Methods: Hepatocellular carcinoma HepG2 cell was cultured and injectedsubcutaneously to form the tumor-supplying mice. The tumor bits from the tumor-supplying mice wereimplanted under the envelope of the mice liver and induced by abdominal chemotherapy withPharmorubicin. Physical examination, ultrasonography, spiral CT and operative inspection were usedto examine tumor progression. RT-PCR and immunohistochemistry were adopted to detect the expressionof mdr1-mRNA and its encoded protein P-gp protein (P-gp). Results: There was no operative dead, therate of implanting tumor successfully was 88% (22/25), the rate of implanting secondly successfullywas 100% (3/3), and the rate of inducing successfully was 80% (16/20). The expression of mdrl-mRNAand the P-gp in the inducing group was 23 folds and 13 folds in the control group respectively.Conclusion: We have established an in vivo model of mdr using nude mice transplanted with orthotopicliver neoplasm coupled to chemotherapy.展开更多
According to the Chapman multi-scale rock physical model, the seismic response characteristics vary for different fluid-saturated reservoirs. For class I AVO reservoirs and gas-saturation, the seismic response is a hi...According to the Chapman multi-scale rock physical model, the seismic response characteristics vary for different fluid-saturated reservoirs. For class I AVO reservoirs and gas-saturation, the seismic response is a high-frequency bright spot as the amplitude energy shifts. However, it is a low-frequency shadow for the Class III AVO reservoirs saturated with hydrocarbons. In this paper, we verified the high-frequency bright spot results of Chapman for the Class I AVO response using the frequency-dependent analysis of a physical model dataset. The physical model is designed as inter-bedded thin sand and shale based on real field geology parameters. We observed two datasets using fixed offset and 2D geometry with different fluid- saturated conditions. Spectral and time-frequency analyses methods are applied to the seismic datasets to describe the response characteristics for gas-, water-, and oil-saturation. The results of physical model dataset processing and analysis indicate that reflection wave tuning and fluid-related dispersion are the main seismic response characteristic mechanisms. Additionally, the gas saturation model can be distinguished from water and oil saturation for Class I AVO utilizing the frequency-dependent abnormal characteristic. The frequency-dependent characteristic analysis of the physical model dataset verified the different spectral response characteristics corresponding to the different fluid-saturated models. Therefore, by careful analysis of real field seismic data, we can obtain the abnormal spectral characteristics induced by the fluid variation and implement fluid detection using seismic data directly.展开更多
AIM: To investigate the effect of synbiotics, i.e. probiotics and prebiotics mixture, on the gut microbial ecology and digestive enzyme activities in rats. METHODS: Forty-eight SD rats weighing about 280 g were used i...AIM: To investigate the effect of synbiotics, i.e. probiotics and prebiotics mixture, on the gut microbial ecology and digestive enzyme activities in rats. METHODS: Forty-eight SD rats weighing about 280 g were used in this study. Rats were divided into three groups according to the contents of probiotics and prebiotics mixture in the feed as control, low and high dose groups. The duration of the experiment was 8 wk. RESULTS: Compared with the control group, thefecal Lactobacillus and Bifidobacterium counts were significantly increased and the fecal Coliform organism counts were markedly reduced in the low and high dose groups. Concerning the digestive enzyme activity of jejunum, only lactase activity increased in low dose group. However, significant increase of lipase, lactase, sucrase, and isomaltase activities were observed in high dose group.CONCLUSION: Intake of low and high dosages of probiotics and prebiotics mixture significantly improved the ecosystem of the intestinal tract by increasing the probiotics population and digestive enzyme activities in rats.展开更多
AIM： To establish a highly reproducible animal model of acute liver failure （ALF）, for assessing the effect of bioartificial liver support system （BALSS）. METHODS： A two-phase complete liver devascularization pr...AIM： To establish a highly reproducible animal model of acute liver failure （ALF）, for assessing the effect of bioartificial liver support system （BALSS）. METHODS： A two-phase complete liver devascularization procedure was performed in eight Ioco-hybrid pigs. Blood biochemical index and liver biopsy were studied every 2 h after surgery, and survival time was recorded. The BALSS constructed with high volume recirculating technique was a hollow fiber circulating system consisting of a hepatocyte reactor-hollow fiber module inoculated with microcarrieradhering hepatocytes, and a double pump, heparinized, thermostabilized, micro-capsulized activated carbonadsorbing plasmapheresis system. Twelve pigs undergoing two-phase surgery were randomized into： control group （perfused without hepatocytes, n = 6） and treatment group （perfused with hepatocytes, n = 6）. Intergroup liver biochemical indexes, survival time, and liver pathological changes were analyzed at regular intervals. RESULTS： Two-phase surgery was performed in all the experimental pigs, and there was no obvious difference between their biochemical indexes. After 3 h of phase II surgery, ammonia （Amm） increased to （269＋37）μmol/L. After 5 h of the surgery, fibrinogen （Fib） decreased to （1.5±0.2） g/L. After 7 h of the surgery, ALT, AST, Tbil and PT were （7.6±1.8） nka/L, （40±5） nka/L, （55±8）μmol/L and （17.5±1.7） nka/L respectively. After 9 h of surgery, ALB and Cr were （27±4） g/L and （87±9）μmol/L. After 13 h of surgery, BUN was （3.5±0.9） μmol/L. All the above values were different from those determined before surgery. Survival time of pigs averaged 13.5±1.4 h. ALF pigs in the other group were treated with BALSS. The comparison analysis between the treated and control animals showed the changes of Tbil, PT, AIb, BUN, Cr, Fib, and Amm （P〈0.01）, but there was no change of ALT and AST. The survival time was statistically different （P〈0.01）, and there was no significant difference in histological changes.CONCLUSION： The porcine ALF model established by two-phase devascularized surgery is valid and reproducible. The hollow fiber BALSS can meet the needs of life support and is effective in treating ALF.展开更多
This study aimed to evaluate the feasibility and safety of a novel stent manufactured by metal injection molding(MIM)in clinical practice through animal experiments.Vessel stents were prepared using powder injection m...This study aimed to evaluate the feasibility and safety of a novel stent manufactured by metal injection molding(MIM)in clinical practice through animal experiments.Vessel stents were prepared using powder injection molding technology to considerably improve material utilization.The influence of MIM carbon impurity variation on the mechanical properties and corrosion resistance of 316L stainless steel was studied.In vitro cytotoxicity and animal transplantation tests were also carried out to evaluate the safety of MIM stents.The results showed that the performance of 316L stainless steel was very sensitive to the carbon content.Carbon fluctuations should be precisely controlled during MIM.All MIM stents were successfully implanted into the aortas of the dogs,and the MIM 316L stents had no significant cytotoxicity.The novel intravascular stent manufactured using MIM can maintain a stable form and structure with fast endothelialization of the luminal surface of the stent and ensure long-term patency in an animal model.The novel intravascular stent manufactured using MIM demonstrates favorable structural,physical,and chemical stability,as well as biocompatibility,offering promising application in clinical practice.展开更多
Objective To identify the genetype of the PS1/APP double transgenie mouse model, then to analyse the histopathological changes in the brain and compare the differences between the transgenie mice models and Aβ1-40-in...Objective To identify the genetype of the PS1/APP double transgenie mouse model, then to analyse the histopathological changes in the brain and compare the differences between the transgenie mice models and Aβ1-40-injeeted rats models of Alzheimer disease. Methods The modified congo red staining, Nissl＇s staining and immunohistology staining was used to observe the Aβ deposits, activation of astrocyte respectively. Results ①The PS1/APP transgenic mouse extensively displayed Aβ deposits in the cortex and hippocampal structures, and GFAP positive cells were aggregated in mass and surrounded the congo red-positive plaque. ②The Aβ1-40-intrahippocmnpal-injeeted rat model showed the Aβ plaque deposits in the dentate gyrus of the hippocampus, with the astrocyte surrounded. The neurons loss was significant in the injection point and pin hole of injection with Nissl＇s staining methods. GFAP-positive cells increased significantly compared with the uninjected lateral of the hippocampus. Conclusion Although Aβ1-40-injected rat models could simulate some characteristic pathological features of human Alzheimer diseases, Aβ deposits and neurons loss in partial hippocampal, it would not simulate the progressive degenenration in the brain of AD. The double transgenie PS1/APP mice could simulate the specific pathogenesis and progressive changes of AD, mainly is Aβ deposits and the spongiocyte response , while no neurons loss were observed in this model.展开更多
文摘A井区位于鄂尔多斯盆地西部,是长庆油田的重要油气产区之一,马家沟组是该井区的主力油气储层,属低孔、低渗储层。论文通过常规物性实验、压汞实验和铸体薄片观察测定实验对所取的260余块岩心样品进行检测分析,得到了A井区马五段的孔渗值、孔隙特征和储层矿物成分以及孔隙类型情况。研究表明:马五段储层孔隙度平均值为3.78%,78.3%样品的渗透率小于0.5 m D,测得所选样品平均孔喉半径值为5.17μm,而且分选性差。马五段储层为碳酸盐岩,所含矿物类型有白云石、方解石、泥质、硅质、伊利石和黄铁矿等,镜下观察到的孔隙类型有溶缝、溶孔、溶洞、膏模孔及构造裂隙,常伴随不同矿物的结晶充填。
基金This project was supported by a grant from the Medical Science and Technique Foundation of Ministry of Health of China(No. Wkz-2000-1-15).
文摘To develop a new method of inducing mdrl multidrug resistance by establishinga nude mice model of orthotopic transplantation of liver carcinoma by sporadic abdominalchemotherapy at intervals. Methods: Hepatocellular carcinoma HepG2 cell was cultured and injectedsubcutaneously to form the tumor-supplying mice. The tumor bits from the tumor-supplying mice wereimplanted under the envelope of the mice liver and induced by abdominal chemotherapy withPharmorubicin. Physical examination, ultrasonography, spiral CT and operative inspection were usedto examine tumor progression. RT-PCR and immunohistochemistry were adopted to detect the expressionof mdr1-mRNA and its encoded protein P-gp protein (P-gp). Results: There was no operative dead, therate of implanting tumor successfully was 88% (22/25), the rate of implanting secondly successfullywas 100% (3/3), and the rate of inducing successfully was 80% (16/20). The expression of mdrl-mRNAand the P-gp in the inducing group was 23 folds and 13 folds in the control group respectively.Conclusion: We have established an in vivo model of mdr using nude mice transplanted with orthotopicliver neoplasm coupled to chemotherapy.
基金supported by the National Science and Technology Major Project (No. 2011ZX05019-008)the National Natural Science Foundation of China (No. 41074080)+1 种基金the Science Foundation of China University of Petroleum, Beijing (No. KYJJ2012-05-11)supported by the CNPC international collaboration program through the Edinburgh Anisotropy Project (EAP) of the British Geological Survey (BGS) and the CNPC Key Geophysical Laboratory at the China University of Petroleum and CNPC geophysical prospecting projects for new method and technique research
文摘According to the Chapman multi-scale rock physical model, the seismic response characteristics vary for different fluid-saturated reservoirs. For class I AVO reservoirs and gas-saturation, the seismic response is a high-frequency bright spot as the amplitude energy shifts. However, it is a low-frequency shadow for the Class III AVO reservoirs saturated with hydrocarbons. In this paper, we verified the high-frequency bright spot results of Chapman for the Class I AVO response using the frequency-dependent analysis of a physical model dataset. The physical model is designed as inter-bedded thin sand and shale based on real field geology parameters. We observed two datasets using fixed offset and 2D geometry with different fluid- saturated conditions. Spectral and time-frequency analyses methods are applied to the seismic datasets to describe the response characteristics for gas-, water-, and oil-saturation. The results of physical model dataset processing and analysis indicate that reflection wave tuning and fluid-related dispersion are the main seismic response characteristic mechanisms. Additionally, the gas saturation model can be distinguished from water and oil saturation for Class I AVO utilizing the frequency-dependent abnormal characteristic. The frequency-dependent characteristic analysis of the physical model dataset verified the different spectral response characteristics corresponding to the different fluid-saturated models. Therefore, by careful analysis of real field seismic data, we can obtain the abnormal spectral characteristics induced by the fluid variation and implement fluid detection using seismic data directly.
基金Supported by Viva Life Science/Westar Nutrition, Costa Mesa,CA, United States
文摘AIM: To investigate the effect of synbiotics, i.e. probiotics and prebiotics mixture, on the gut microbial ecology and digestive enzyme activities in rats. METHODS: Forty-eight SD rats weighing about 280 g were used in this study. Rats were divided into three groups according to the contents of probiotics and prebiotics mixture in the feed as control, low and high dose groups. The duration of the experiment was 8 wk. RESULTS: Compared with the control group, thefecal Lactobacillus and Bifidobacterium counts were significantly increased and the fecal Coliform organism counts were markedly reduced in the low and high dose groups. Concerning the digestive enzyme activity of jejunum, only lactase activity increased in low dose group. However, significant increase of lipase, lactase, sucrase, and isomaltase activities were observed in high dose group.CONCLUSION: Intake of low and high dosages of probiotics and prebiotics mixture significantly improved the ecosystem of the intestinal tract by increasing the probiotics population and digestive enzyme activities in rats.
基金Supported by the Key Technologies R and D Program of Guangdong Province during the 10~(th) Five-Year Plan Period, No. 2002A3020206
文摘AIM： To establish a highly reproducible animal model of acute liver failure （ALF）, for assessing the effect of bioartificial liver support system （BALSS）. METHODS： A two-phase complete liver devascularization procedure was performed in eight Ioco-hybrid pigs. Blood biochemical index and liver biopsy were studied every 2 h after surgery, and survival time was recorded. The BALSS constructed with high volume recirculating technique was a hollow fiber circulating system consisting of a hepatocyte reactor-hollow fiber module inoculated with microcarrieradhering hepatocytes, and a double pump, heparinized, thermostabilized, micro-capsulized activated carbonadsorbing plasmapheresis system. Twelve pigs undergoing two-phase surgery were randomized into： control group （perfused without hepatocytes, n = 6） and treatment group （perfused with hepatocytes, n = 6）. Intergroup liver biochemical indexes, survival time, and liver pathological changes were analyzed at regular intervals. RESULTS： Two-phase surgery was performed in all the experimental pigs, and there was no obvious difference between their biochemical indexes. After 3 h of phase II surgery, ammonia （Amm） increased to （269＋37）μmol/L. After 5 h of the surgery, fibrinogen （Fib） decreased to （1.5±0.2） g/L. After 7 h of the surgery, ALT, AST, Tbil and PT were （7.6±1.8） nka/L, （40±5） nka/L, （55±8）μmol/L and （17.5±1.7） nka/L respectively. After 9 h of surgery, ALB and Cr were （27±4） g/L and （87±9）μmol/L. After 13 h of surgery, BUN was （3.5±0.9） μmol/L. All the above values were different from those determined before surgery. Survival time of pigs averaged 13.5±1.4 h. ALF pigs in the other group were treated with BALSS. The comparison analysis between the treated and control animals showed the changes of Tbil, PT, AIb, BUN, Cr, Fib, and Amm （P〈0.01）, but there was no change of ALT and AST. The survival time was statistically different （P〈0.01）, and there was no significant difference in histological changes.CONCLUSION： The porcine ALF model established by two-phase devascularized surgery is valid and reproducible. The hollow fiber BALSS can meet the needs of life support and is effective in treating ALF.
基金the Major Project of the Ministry of Science and Technology of Changsha,China(No.kh2003014)the Hunan Provincial Natural Science Foundation,China(Nos.2018JJ2584,2018JJ3507)+1 种基金the Beijing Municipal Science and Technology Comission,China(No.D171100002917004)the Guangxi Science and Technology Plan Project,China(No.AD16380019).
文摘This study aimed to evaluate the feasibility and safety of a novel stent manufactured by metal injection molding(MIM)in clinical practice through animal experiments.Vessel stents were prepared using powder injection molding technology to considerably improve material utilization.The influence of MIM carbon impurity variation on the mechanical properties and corrosion resistance of 316L stainless steel was studied.In vitro cytotoxicity and animal transplantation tests were also carried out to evaluate the safety of MIM stents.The results showed that the performance of 316L stainless steel was very sensitive to the carbon content.Carbon fluctuations should be precisely controlled during MIM.All MIM stents were successfully implanted into the aortas of the dogs,and the MIM 316L stents had no significant cytotoxicity.The novel intravascular stent manufactured using MIM can maintain a stable form and structure with fast endothelialization of the luminal surface of the stent and ensure long-term patency in an animal model.The novel intravascular stent manufactured using MIM demonstrates favorable structural,physical,and chemical stability,as well as biocompatibility,offering promising application in clinical practice.
基金This project was supported by the National Natural Science Foundation of China （ No. 30100087, 30500148, 30571770）funded by the Collaborating Research Fund for Young Scholars from Abroad of National Natural Science Foundation of China （ No. 30228018 ）.
文摘Objective To identify the genetype of the PS1/APP double transgenie mouse model, then to analyse the histopathological changes in the brain and compare the differences between the transgenie mice models and Aβ1-40-injeeted rats models of Alzheimer disease. Methods The modified congo red staining, Nissl＇s staining and immunohistology staining was used to observe the Aβ deposits, activation of astrocyte respectively. Results ①The PS1/APP transgenic mouse extensively displayed Aβ deposits in the cortex and hippocampal structures, and GFAP positive cells were aggregated in mass and surrounded the congo red-positive plaque. ②The Aβ1-40-intrahippocmnpal-injeeted rat model showed the Aβ plaque deposits in the dentate gyrus of the hippocampus, with the astrocyte surrounded. The neurons loss was significant in the injection point and pin hole of injection with Nissl＇s staining methods. GFAP-positive cells increased significantly compared with the uninjected lateral of the hippocampus. Conclusion Although Aβ1-40-injected rat models could simulate some characteristic pathological features of human Alzheimer diseases, Aβ deposits and neurons loss in partial hippocampal, it would not simulate the progressive degenenration in the brain of AD. The double transgenie PS1/APP mice could simulate the specific pathogenesis and progressive changes of AD, mainly is Aβ deposits and the spongiocyte response , while no neurons loss were observed in this model.
