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论穴居两栖爬行动物的原始性与特化性 被引量:1
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作者 边树蕊 《产业与科技论坛》 2012年第11期96-97,共2页
生物在环境中生存,其器官和系统适应环境而演化。虽然在提到某一动物类群时,我们总是用"进化"与否来衡量,然而很多动物兼有进化和原始两方面特征的情况。这种情况在穴居两栖爬行类动物身上有着集中体现。穴居两栖爬行类动物... 生物在环境中生存,其器官和系统适应环境而演化。虽然在提到某一动物类群时,我们总是用"进化"与否来衡量,然而很多动物兼有进化和原始两方面特征的情况。这种情况在穴居两栖爬行类动物身上有着集中体现。穴居两栖爬行类动物有着诸多共同特征,譬如附肢骨退化和视觉器官退化、皮肤特化、生殖行为复杂多样等,这些特征有些被视为原始性的体现,有些则较为进化。然而,"存在即合理",本文将横向对这些特征进行总结,并结合穴居动物所处环境对它们出现的原因进行分析。 展开更多
关键词 穴居 两栖爬行动物 进化特征 原始 特化性
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双翅目昆虫复眼性特化光感受器的比较研究 被引量:3
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作者 吴卫国 杨智勇 +2 位作者 吴炜 冯汉平 吴梅英 《昆虫学报》 CAS CSCD 北大核心 1999年第1期7-11,共5页
雄性双翅目昆虫,包括家蝇Muscadomestica、丽蝇Caliphoraerythrocephala、华虻Tabanusmandarinus和憎黄虻AtylotusmiserSzilady,其复眼性特化光感受器... 雄性双翅目昆虫,包括家蝇Muscadomestica、丽蝇Caliphoraerythrocephala、华虻Tabanusmandarinus和憎黄虻AtylotusmiserSzilady,其复眼性特化光感受器中央小网膜细胞R7的分布从背区扩展到腹区。在雄性家蝇、华虻和憎黄虻复眼中,性特化光感受器中央小网膜细胞R7的感杆延伸到基底膜,并同中央小网膜细胞R8的感杆并列排列。但在雄性丽蝇复眼中,性特化光感受器中央小网膜细胞R7的感杆不延伸到基底膜。在雌性双翅目昆虫复眼中,性特化光感受器中央小网膜细胞R7仅仅分布在复眼的腹区,其数量比中央小网膜细胞R8少得多。 展开更多
关键词 双翅目 昆虫 复眼 特化光感受器
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家蝇复眼性特化光感受器的分布及其特征 被引量:2
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作者 吴卫国 石淑珍 吴盛立 《解剖学报》 CAS 1986年第1期98-100,126-127,共5页
1.雄性家蝇复眼中央小网膜细胞R_(7),可分成两种类型:一种小网膜细胞R_(7a)和另一种小网膜细胞R_(7b)。R_(7a)的细胞体、感杆和轴突较大,它与外周小网膜细胞R1~6相同,其轴突终止在第一视神经节(薄板);而R_(7b)的细胞体、感杆和轴突较小... 1.雄性家蝇复眼中央小网膜细胞R_(7),可分成两种类型:一种小网膜细胞R_(7a)和另一种小网膜细胞R_(7b)。R_(7a)的细胞体、感杆和轴突较大,它与外周小网膜细胞R1~6相同,其轴突终止在第一视神经节(薄板);而R_(7b)的细胞体、感杆和轴突较小,它与中央小网膜细胞R8相同,其轴突终止在第二视神经节(髓)。2.雄性家蝇复眼中央小网膜细胞R_(7a)主要分布在复眼的背区,在腹区中较少,仅分布在第一、二排小眼中;但在雌性家蝇复眼中仅有R_(7b)型中央小网膜细胞。 展开更多
关键词 家蝇 复限 特化 光感受器
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Aging Characteristics of Lithium-Ion Battery Under Fast Charging Based on Electrochemical-thermalmechanical Coupling Model
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作者 Dong-Xu Zuo Pei-Chao Li 《电化学(中英文)》 CAS 北大核心 2024年第9期10-24,共15页
The aging characteristics of lithium-ion battery(LIB)under fast charging is investigated based on an electrochemical-thermal-mechanical(ETM)coupling model.Firstly,the ETM coupling model is established by COMSOL Multip... The aging characteristics of lithium-ion battery(LIB)under fast charging is investigated based on an electrochemical-thermal-mechanical(ETM)coupling model.Firstly,the ETM coupling model is established by COMSOL Multiphysics.Subsequently,a long cycle test was conducted to explore the aging characteristics of LIB.Specifically,the effects of charging(C)rate and cycle number on battery aging are analyzed in terms of nonuniform distribution of solid electrolyte interface(SEI),SEI formation,thermal stability and stress characteristics.The results indicate that the increases in C rate and cycling led to an increase in the degree of nonuniform distribution of SEI,and thus a consequent increase in the capacity loss due to the SEI formation.Meanwhile,the increases in C rate and cycle number also led to an increase in the heat generation and a decrease in the heat dissipation rate of the battery,respectively,which result in a decrease in the thermal stability of the electrode materials.In addition,the von Mises stress of the positive electrode material is higher than that of the negative electrode material as the cycling proceeds,with the positive electrode material exhibiting tensile deformation and the negative electrode material exhibiting compressive deformation.The available lithium ion concentration of the positive electrode is lower than that of the negative electrode,proving that the tensile-type fracture occurring in the positive material under long cycling dominated the capacity loss process.The aforementioned studies are helpful for researchers to further explore the aging behavior of LIB under fast charging and take corresponding preventive measures. 展开更多
关键词 Lithium-ion battery Aging characteristics Fast charging Electrochemical-thermal-mechanical coupling model
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Optimality of Group Testing with Differential Misclassification
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作者 LI Yiming ZHANG Hong LIU Aiyi 《应用概率统计》 CSCD 北大核心 2024年第4期644-662,共19页
Group testing is a method that can be used to estimate the prevalence of rare infectious diseases,which can effectively save time and reduce costs compared to the method of random sampling.However,previous literature ... Group testing is a method that can be used to estimate the prevalence of rare infectious diseases,which can effectively save time and reduce costs compared to the method of random sampling.However,previous literature only demonstrated the optimality of group testing strategy while estimating prevalence under some strong assumptions.This article weakens the assumption of misclassification rate in the previous literature,considers the misclassification rate of the infected samples as a differentiable function of the pool size,and explores some optimal properties of group testing for estimating prevalence in the presence of differential misclassification conforming to this assumption.This article theoretically demonstrates that the group testing strategy performs better than the sample by sample procedure in estimating disease prevalence when the total number of sample pools is given or the size of the test population is determined.Numerical simulation experiments were conducted to evaluate the performance of group tests in estimating prevalence in the presence of dilution effect. 展开更多
关键词 group testing sensitivity SPECIFICITY dilution effect differential misclassification PREVALENCE
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Analysis of Antioxidant Characteristics in Seedling Roots of Sweetpotato(Ipomoea batatas(L.) Lam.) with Different Drought Tolerance under Simulated Drought Stress 被引量:3
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作者 姜瑶 汪宝卿 +4 位作者 解备涛 张海燕 段文学 王庆美 张立明 《Agricultural Science & Technology》 CAS 2016年第2期245-250,255,共7页
[Objective] Jishu 21 with strong drought tolerance and Jizishu 1 with weak drought tolerance were chosen to reveal antioxidant characteristics in seedling roots of sweetpotato with different drought tolerance. [Method... [Objective] Jishu 21 with strong drought tolerance and Jizishu 1 with weak drought tolerance were chosen to reveal antioxidant characteristics in seedling roots of sweetpotato with different drought tolerance. [Method] Active oxygen, antioxidant substances and antioxidant enzymes in seedling roots of sweetpotato with different drought tolerance were analyzed by hydroponics with PEG-6000 for simulating drought stress in the experiment. [Result] The results showed that under drought stress, H202 content and formation rate of 02_ in seedling roots of Jizishul were higher than Jishu 21 by 1.9% and 102.6%, respectively, the contents of Vc in seed- ing roots of Jizishu 1 and Jizishu 1 increased by 25.3% and 81.1%, respectively, and the contents of polyphenols increased by 24.8% and 37.6% in the two vari- eties, respectively. The increase amplitudes of Jizishu 1 were higher than Jishu 21 in the above indexes. Antioxidant enzyme activities were higher in seedling roots of Jisl^u 21 and Jizishul under drought stress than normal treatments; and among them, POD activity was the highest, while PPO activity was the lowest in all an- tioxidant enzymes. The increases of SOD, POD, APX and PPO activities in Jishu 21 were twice of those in Jizishul. The bands of SOD3, POD2, CAT1, APX2 and PPO2 changed obviously between the drought treatments of the two varieties. [Con- clusion] In conclusion, stronger drought tolerance in sweetpotato results in lower in- crease amplitudes of H202 content, formation rate of 02- and contents of Vc and polyphenols under drought stress, and the activities of antioxidant enzymes are stronger in seeding roots under drought stress; and the differences in antioxidant enzymes are mainly related to the changes of isoenzymes including SOD3, POD2, CAT1. APX2 and PPO2. 展开更多
关键词 Drought SWEETPOTATO Root Antioxidant enzyme ISOENZYME
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Chlorophyll Fluorescence and Membrane Lipid Peroxidation in the Flag Leaves of Different High Yield Rice Variety at Late Stage of Development Under Natural Condition 被引量:22
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作者 李霞 焦德茂 +1 位作者 刘友良 黄雪清 《Acta Botanica Sinica》 CSCD 2002年第4期413-421,共9页
With indica ( Oryza sativa L.) hybrid Shanyou 63 as control, the hybrid rice varieties including Peiai 64S/E32, Peiai 64S/9311, X07S/Zihui 100, Guangyou 881 and japonica 9516 were used to study changes of chlorophyll ... With indica ( Oryza sativa L.) hybrid Shanyou 63 as control, the hybrid rice varieties including Peiai 64S/E32, Peiai 64S/9311, X07S/Zihui 100, Guangyou 881 and japonica 9516 were used to study changes of chlorophyll content, photosynthetic response to light intensity and temperature, chlorophyll fluorescence characteristics and membrane lipid peroxidation in their flag leaves at the late stage of development under natural conditions in Nanjing. The results were as follows:. primary photochemical efficiency of PS II ( F-v / F-m), quantum yield of linear electron transport of PS II (phi(PSII)), electron transfer rate (ETR) in these rice varieties decreased with their decrease of chlorophyll content during this period. This kind of impediment to energy conversion induced the transfer of excessive energy to the reducing side of PS I, hence the accumulation of O-2(radical anion) and peroxidation of membrane lipid, and resulting in the accumulation of malondialdehyde (MDA), that is the destroys of photosynthetic pigments and membranes and the consequent, premature senescence. This phenomenon is variable conspicuously in different rice varieties. Under natural condition in Nanjing, F-v/F-m, phi(PSII), ETR and quenching coefficient ( qP) in japonica 9516 tolerant to photooxidation decreased less and the conversion capacity of light energy was stable, premature senescence was unlikely, and consequently the seed-setting rate was higher. While F-v/F-m, phi(PSII), ETR and photochemical qP in Shanyou 63 sensitive to photooxidation decreased more and therefore premature senescence was easy to happen, thus the seed-setting rate and yield were all reduced. The tolerance to photooxidation and premature senescence in other hybrids derived from typical two line or three line crossing laid in the middle. From the rice breeding for super-high-yield, on the basis of the good plant-type of current rice, considering both hybrid vigor and the prevention premature senescence, it would be a notable strategy to use japonica maternal line or maternal. lines with some japonica genotype as the sterile lines in rice breeding. 展开更多
关键词 premature senescence PHOTOOXIDATION fluorescence characteristics lipid peroxidation RICE
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Variation of Nitrogen Uptake and Utilization Efficiency of Mid-Season Hybrid Rice at Different Ecological Sites under Different Nitrogen Application Levels 被引量:3
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作者 徐富贤 熊洪 +4 位作者 张林 郭晓艺 朱永川 周兴兵 刘茂 《Agricultural Science & Technology》 CAS 2011年第7期1001-1009,1012,共10页
[Objective] The study aimed at investigating the effects of different geographic sites,soil chemical characteristics and nitrogen application levels on nitrogen accumulation and distribution in different organs and ut... [Objective] The study aimed at investigating the effects of different geographic sites,soil chemical characteristics and nitrogen application levels on nitrogen accumulation and distribution in different organs and utilization efficiency for mid-season hybrid rice.[Method] By using mid-season rice varieties II-you 7 and Yuxiangyou203 as the experimental materials,field experiment was conducted at seven ecological sites in four provinces or cities in Southwestern China in 2009.A total of four nitrogen application levels were set as follows:by using 75 kg/hm2 of P2O5 and 75 kg/hm2 of K2O as the base fertilizer,extra 0,90,150 and 210 kg/hm2 of nitrogen fertilizer(in which,base fertilizer,base-tillering fertilizer and base-earing fertilizer respectively accounted for 60%,20% and 20%.) was applied,respectively.In the split-plot design,fertilizer was considered as the main factor while rice variety was taken as the secondary factor.A total of eight treatments were set with three replications.[Result] Highly significant differences of grain yield were found among seven locations,two varieties,four nitrogen application levels,interactions of site × variety and site × nitrogen application level,but the interaction of variety ×nitrogen application level had no significant influence on rice yield.There were highly significant effects of site,varieties and nitrogen application level on dry matter production,nitrogen content,nitrogen utilization efficiency.Highly significant negative correlations between uptake efficiency and utilization efficiency for nitrogen were found;and multiple stepwise regression analysis showed that nitrogen uptake-utilization efficiency were significantly influenced by different ecological sites,chemical quality of soil and the levels of nitrogen application.[Conclusion] The research will provide theoretical and practical basis for the highly efficient application of nitrogen in mid-season hybrid rice cultivation. 展开更多
关键词 Mid-season hybrid rice Ecological site Soil chemical characteristics Nitrogen application level Nitrogen uptake and utilization efficiency
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Available parking space occupancy change characteristics and short-term forecasting model 被引量:5
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作者 季彦婕 王炜 邓卫 《Journal of Southeast University(English Edition)》 EI CAS 2007年第4期604-608,共5页
Based on an available parking space occupancy (APSO) survey conducted in Nanjing, China, an APSO forecasting model is proposed. The APSO survey results indicate that the time series of APSO with different time-secti... Based on an available parking space occupancy (APSO) survey conducted in Nanjing, China, an APSO forecasting model is proposed. The APSO survey results indicate that the time series of APSO with different time-sections are periodical and self-similar, and the fluctuation of the APSO increases with the decrease in time-sections. Taking the short-time change behavior into account, an APSO forecasting model combined wavelet analysis and a weighted Markov chain is presented. In this model, an original APSO time series is first decomposed by wavelet analysis, and the results include low frequency signals representing the basic trends of APSO and several high frequency signals representing disturbances of the APSO. Then different Markov models are used to forecast the changes of low and high frequency signals, respectively. Finally, integrating the predicted results induces the final forecasted APSO. A case study verifies the applicability of the proposed model. The comparisons between measured and forecasted results show that the model is a competent model and its accuracy relies on real-time update of the APSO database. 展开更多
关键词 available parking space occupancy change characteristics short-term forecasting wavelet analysis weighted Markov chain
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Influence of rapid solidification on Sn-8Zn-3Bi alloy characteristics and microstructural evolution of solder/Cu joints during elevated temperature aging 被引量:2
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作者 赵国际 文光华 盛光敏 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2017年第1期234-240,共7页
The effects of rapid solidification on the microstructure and melting behavior of the Sn-8Zn-3Bi alloy were studied. The evolution of the microstructuraI characteristics of the solder/Cu joint after an isothermal agin... The effects of rapid solidification on the microstructure and melting behavior of the Sn-8Zn-3Bi alloy were studied. The evolution of the microstructuraI characteristics of the solder/Cu joint after an isothermal aging at 150 ℃ was also analyzed to evaluate the interconnect reliability. Results showed that the Bi in Sn-8Zn-3Bi solder alloy completely dissolved in the Sn matrix with a dendritic structure after rapid solidification. Compared with as-solidified Sn-8Zn-3Bi solder alloy, the melting temperature of the rapid solidified alloy rose to close to that of the Sn-Zn eutectic alloy due to the extreme dissolution of Bi in Sn matrix. Meanwhile, the adverse effect on melting behavior due to Bi addition was decreased significantly. The interfacial intermetallic compound (IMC) layer of the solder/Cu joint was more compact and uniform. Rapid solidification process obviously depressed the formation and growth of the interfacial IMC during the high-temperature aging and improved the high-temperature stability of the Sn-8Zn-3Bi solder/Cu joint. 展开更多
关键词 rapid solidification Sn-8Zn-3Bi solder melting characteristic AGING microstructural evolution
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Effects of Storage Temperature on Pasting Properties of Brown Rice 被引量:1
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作者 宋伟 刘璐 +1 位作者 支永海 陈瑞 《Agricultural Science & Technology》 CAS 2010年第11期21-24,共4页
[Objective] The aim of this study was to explore the effects of storage temperature on pasting properties of brown rice.[Method] Rapid viscosity analyzer was used to determine the viscosity of brown rice under differe... [Objective] The aim of this study was to explore the effects of storage temperature on pasting properties of brown rice.[Method] Rapid viscosity analyzer was used to determine the viscosity of brown rice under different storage temperatures(30,25,20 and 15 ℃).[Result] The peak viscosity value increased with storage time prolonging.The higher the storage temperature was,the more rapidly the increasing of peak viscosity value;different storage temperatures and storage time showed significant effects on peak viscosity value of brown rice;the difference on peak viscosity value of brown rice under different storage temperatures was significant.The hot viscosity value of brown rice stored under 30 and 25 ℃ gradually increased with the storage time prolonging;and storage time and storage temperature showed significant effects on hot viscosity value;there was extremely significant difference on hot viscosity value of brown rice under 30,25 and 20,15 ℃.The breakdown value showed a first increasing and then decreasing trend with the storage time prolonging;storage temperature and storage time had extremely significant effect on the breakdown value of brown rice;the breakdown value of brown rice among each storage temperature was extremely significant.The final viscosity of brown rice increased with storage time prolonging.The higher the temperature was,the higher the increasing rate of final viscosity;storage time and temperature showed extremely significant effect on the final viscosity of brown rice;the difference on final viscosity among different storage temperature treatments was extremely significant.The changes on setback value of brown rice under different storage temperature treatments were different with the storage time prolonging;and the storage temperature showed extremely significant effect on the setback value of brown rice.[Conclusion] The study had provided theoretical basis for the storage of brown rice. 展开更多
关键词 TEMPERATURE Pasting properties Brown rice STORAGE
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Research on Nourishing Compositions of Fagopyrum tataricum 被引量:2
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作者 宋毓雪 黄凯丰 《Agricultural Science & Technology》 CAS 2010年第9期115-117,共3页
[Objective] The aim was to identify the nourishing compositions of Fagopyrum tataricum and screen high-quality buckwheat materials.[Method]A total of 10 species of buckwheat including T32,T31,T30,T14,T28,T23,T24,Jiuji... [Objective] The aim was to identify the nourishing compositions of Fagopyrum tataricum and screen high-quality buckwheat materials.[Method]A total of 10 species of buckwheat including T32,T31,T30,T14,T28,T23,T24,Jiujiang Buckwheat,Weiku 2 and WK-1 were employed as experimental materials,and the contents of the nutritional components(including total sugar,reducing sugar,starch,amylose,amylopectin and protein),flavonoids content,physical and chemical characteristics(such as expansive force,water holding capacity)of them were respectively measured.[Result]There were significant differences in contents of soluble protein,total sugar,reduced sugar,amylase and expansive force among the cultivars,but the differences in contents of total starch,amylopectin,flavonoids content and water holding capacity were not significant.[Conclusion]The starch content in seeds of buckwheat was relatively high,and amylopectin was the main composition. 展开更多
关键词 BUCKWHEAT Nourishing compositions Physical and chemical characteristics
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Physiological Basis of Photosynthetic Tolerance to Photooxidation and Shading in Rice 被引量:13
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作者 李霞 焦德茂 《Acta Botanica Sinica》 CSCD 2000年第12期1271-1277,共7页
Thirty genotypes from rice germplasm were identified under photooxidation and shading condition and divided into four basic types : (1) cultivars tolerant to both photooxidation and shading, (2) cultivars tolerant to... Thirty genotypes from rice germplasm were identified under photooxidation and shading condition and divided into four basic types : (1) cultivars tolerant to both photooxidation and shading, (2) cultivars tolerant to photooxidation but sensitive to shading, (3) cultivars tolerant to shading but sensitive to photooxidation, and (4) cultivars sensitive to photooxidation and shading. A comparative study of photosynthetic characteristics of a cultivar (cv. Wuyujing 3) that is tolerant and a cultivar (cv. Xiangxian) that is sensitive to both photoinhibition and shading showed that the photochemical efficiency of PSⅡ ( F v/F m ) and the content of PSⅡ_D1 protein in the tolerant cultivar “Wuyujing 3” decreased less under photoinhibition conditions as compared with “Xiangxian”. Under photooxidation conditions, superoxide dismutase was induced rapidly to a higher level and the active oxygen O - built up to a lower level in “Wuyujing 3” than in “Xiangxian”. At the same time, the photosynthetic rate decreased by 23% in “Wuyujing 3” vs. 64% in “Xiangxian”. Shading (20% natural light) during the booting stage caused only small decreases (7%-13%) in RuBisCO activity and the photosynthetic rate in “Wuyujing 3” but showed marked decreases (57%-64%) in “Xiangxian” which corresponded to the decreases in grain yield in the two cultivars (38% and 73%, respectively). The correlation analysis showed that the tolerance to photooxidation is mainly related to PSⅡ_D1 and that to shading is mainly related to RuBisCO activity. This study provided a simple and effective screening method and physiological basis for crop breeding in enhancing tolerance to both high and low radiation. 展开更多
关键词 PHOTOOXIDATION SHADE RICE PSⅡphotochemical efficiency RuBi0
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Fluidization characteristics of magnetic particles and determination of stable fluidization zone in magnetically fluidized bed 被引量:1
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作者 王迎慧 归柯庭 施明恒 《Journal of Southeast University(English Edition)》 EI CAS 2006年第4期523-527,共5页
To determine and calculate the stable fluidization zone in a magnetically fluidized bed, the fluidization characteristics of magnetic particles are investigated. Four kinds of magnetic particles with different average... To determine and calculate the stable fluidization zone in a magnetically fluidized bed, the fluidization characteristics of magnetic particles are investigated. Four kinds of magnetic particles with different average diameters, ranging from 231 to 512 μm, are fluidized in the presence of magnetic fields with specified values of the intensity in the range of zero to 7330 A/m, and the particle fluidization curves are plotted. For marking the stable fluidization zone in the curves, the minimum bubbling velocities of particles are measured by the pressure-drop fluctuation. Based on the fluidization curves, the influences of the average particle diameter and magnetic field intensity on the zone are analyzed and discussed. A correlation to determine the stable fluidization zone is derived from the experimental data, using three dimensionless numbers, i. e., the ratio of magnetic potential to gravity potential, the Reynolds number and the Archimedes number. Compared with available data reported, it is shown that the correlation is more simplified to predict relative parameters for the bed operating in the state of stable fluidization under reasonable conditions. 展开更多
关键词 magnetically fluidized bed fluidization characteristics stable fluidization minimum bubbling gas velocity pressure-drop fluctuation
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RASSF1A基因启动子甲基化状态与喉癌关系的实验研究 被引量:3
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作者 别远志 孙敬武 李万举 《中国中西医结合耳鼻咽喉科杂志》 2016年第5期335-340,共6页
目的检测喉癌组织的抑制基因RAS相关区域家族1A(Ras associationdomain family 1,isoform A,RASSF1A)甲基化状态,并探讨RASSF1A启动子甲基化与喉癌的关系。方法应用甲基化特异性PCR技术技术检测52例喉癌组织,52例相应癌旁组织及24例对... 目的检测喉癌组织的抑制基因RAS相关区域家族1A(Ras associationdomain family 1,isoform A,RASSF1A)甲基化状态,并探讨RASSF1A启动子甲基化与喉癌的关系。方法应用甲基化特异性PCR技术技术检测52例喉癌组织,52例相应癌旁组织及24例对照组织中RASSFIA基因启动子甲基化状态。按年龄、性别、肿瘤分化程度、肿瘤分期进行分组,分析RASSF1A基因启动子区甲基化情况与各组临床病理特征的关系。结果 1喉癌组织、癌旁组织及对照组织中RASSFIA基因启动子区甲基化的例数分别65.4%、7.7%、0%。喉癌组甲基化发生率显著高于癌旁组和对照组,差异有显著统计学意义(P<0.05),而癌旁组甲基化发生率与对照组比较,差异无统计学意义(P>0.05)。2RASSF1A基因启动子甲基化情况与喉癌患者的年龄、性别及肿瘤的分化程度、及分期情况关系不明显,按上述临床病理特征分组,各组内比较差异均无统计学意义(P>0.05)。结论 1喉癌组织中RASSF1A基因启动子区的甲基化,提示其与喉癌发生关系密切,可能喉癌发生过程中一个重要的分子机制。2不同肿瘤的分化及分期间甲基化程度无明显差异,提示RASSF1A基因发生甲基化可能是喉癌发生中的早期事件。3RASSF1A启动子甲基化有望成为早期诊断喉癌的一个分子标志。甲基化特异性PCR方法是一种快而灵敏的基因甲基化检测方法,有望应用于临床。 展开更多
关键词 喉癌 RASSF1A基因 甲基化 甲基化特异PCR
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Cloning of the Full-length Gene for Tobacco Ethylene Receptor NTHK2 and Characterization of Its Kinase Domain 被引量:2
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作者 张志刚 巩燕 +4 位作者 何新建 王玉军 孙仲序 张劲松 陈受宜 《Acta Botanica Sinica》 CSCD 2003年第1期68-72,共5页
Previously the partial sequence of an ethylene receptor gene NTHK2 was isolated from tobacco (Nicotiana tabacum L. var. Xanthi) plants and it was wound and drought inducible. In the present study full-length cDNA of N... Previously the partial sequence of an ethylene receptor gene NTHK2 was isolated from tobacco (Nicotiana tabacum L. var. Xanthi) plants and it was wound and drought inducible. In the present study full-length cDNA of NTHK2 was cloned by 5'-RACE method. NTHK2 gene has 3 216 bp, with 509 bp of 5'-non-coding region and 427 lip of 3'-non-coding region, and encodes an ethylene-receptor homolog of 760, amino acids. NTHK2 protein has a putative signal peptide, three transmembrane domains, a histidine kinase domain and a receiver domain. In the putative histidine kinase domain, the histidine at the phosphorylation site was replaced by an asparagine. To study the biochemical property of NTHK2, its kinase domain was expressed as a fusion protein with glutathione S-transferase (GST) using yeast Schizzosaccharomyces pombe as an expression system. In vitro kinase assay showed that NTHK2 kinase domain can autophosphorylate in the presence of Mg2+, indicating that NTHK2 may function as a kinase. Further studies will elucidate the function of NTHK2 in plant. 展开更多
关键词 5 '-RACE NTHK2 kinase activity ethylene-receptor
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Synthesis of One-Dimensional ZnO Na norods by Oxidating Zinc Films Deposited with Magnetron Sputtering 被引量:5
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作者 石礼伟 李玉国 +2 位作者 王强 薛成山 王书运 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2004年第10期1211-1214,共4页
One-dimensional ZnO nanorods are synthesized by ox idating thin metal zinc films deposited on Si(111) substrates with radio frequen cy magnetron sputtering.The crystal structure,surface morphology,and optical pro per... One-dimensional ZnO nanorods are synthesized by ox idating thin metal zinc films deposited on Si(111) substrates with radio frequen cy magnetron sputtering.The crystal structure,surface morphology,and optical pro perties of nanorods are investigated.X-ray diffraction(XRD) pattern,scanning el ectron microscopy(SEM),and transmission electron microscopy(TEM) analyses show t hat the synthesized single-crystal ZnO nanorods develop like hairpins along dif ferent radials,with a hexagonal wurtzite structure.The diameters of nanorods ran ge between 30 and 60nm and lengths up to micrometers.Photoluminescence(PL) analy sis shows that,under 280nm light excitation,a strong and sharp near band-edge U V light emission band at 372nm and a relatively weak green deep-level light emi ssion band at 516nm are observed from the ZnO nanorods,which indicates excellent crystallization and optical quality of the fabricated ZnO nanorods. 展开更多
关键词 magnetron sputtering thermal oxidation ZNO nan orods properties
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Screening of a Novel Bioflocculant-producing Strain and Research on Its Flocculation 被引量:4
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作者 周爱华 梁生康 单宝田 《Agricultural Science & Technology》 CAS 2012年第9期1997-2000,2005,共5页
[Objective] This study aimed to screen a bacterial strain capable of producing bioflocculant. [Method] A bacterial strain T-11 capable of producing bioflocculant was isolated from activated sludge. Detailed tests on t... [Objective] This study aimed to screen a bacterial strain capable of producing bioflocculant. [Method] A bacterial strain T-11 capable of producing bioflocculant was isolated from activated sludge. Detailed tests on the morphological, physiological and biochemical characteristics were carried out and identification was performed to identify the strain. Finally, the bioflocculant was isolated and purified, and the flocculating activity and chemical characteristics were measured. [Result] It was identified as Serratia plumuthica based on its morphological, physiological and biochemical characteristics. This strain secreted flocculant best in a culture medium which included sucrose and NaNO3. The maximal cell growth was achieved within 10 h and the flocculating activity paralleled to it. It was found to be effective for flocculation of kaolin suspension, when added at a final concentration of 0.7 mg/L, over a range of pHs (2-7), and temperature (approximately 30-80 ℃). Chemical analysis indicated that the bioflocculant was an acidic polysaccharide consisting of glucose, glucuronic acid and galactose, talose and altrose. Infrared spectrum analysis also revealed typical characteristics of polysaccharides. [Conclusion] The biofloccu- lants produced by strain T-11 can greatly improve the ability of activated sludge to settle. 展开更多
关键词 BIOFLOCCULANT Flocculating activity POLYSACCHARIDE Serratia plumuthica
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Variations in Antioxidase Activities and MDA Content in Potato Tubers Infected by Fusarium trichothecioides 被引量:1
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作者 李凤兰 付瑶 +7 位作者 袁强 吕文河 徐永清 刘荣梅 胡宝忠 陈芾葳 徐媛媛 冯艳忠 《Agricultural Science & Technology》 CAS 2015年第11期2433-2436,共4页
[Objective] This study was conducted to determine the physiological and biochemical characteristics of potato tubers infected by Fusarium trichothecioides. [Method] The soluble protein content, SOD activity, POD activ... [Objective] This study was conducted to determine the physiological and biochemical characteristics of potato tubers infected by Fusarium trichothecioides. [Method] The soluble protein content, SOD activity, POD activity and malondialdehyde (MDA) content of the tubers of two potato varieties Atlantic and Keshan 1 infected by F. trichothecioides were measured 0, 3, 6, 9, 12, 24 and 48 h later. [Result] Com- pared with the control uninfected, the contents of soluble protein and MDA in the tu- bers of both potato varieties infected with F. trichothecioides decreased, while the SOD activity increased, indicating that SOD activity had a certain relationship with the resistance of potato to dry rot. The POD activity in Keshan 1 which is resistant to F. trichothecioides was higher than in Atlantic, which is susceptible to F. trichothe- cioides, indicating that POD activity was closely related with potato's resistance to Fusarium dry rot. [Conclusion] The physiological and biochemical characteristics of potato tubers infected by F. trichothecioides will provide some references for the early waming and prevention of Fusariurn dry rot. 展开更多
关键词 Fusarium dry rot Fusanum tdchothecioides Physiological and biochemi-cal characteristics Antioxidases
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Effects of Sunflower Artificial Aging on Seed Vigor and Physiological Characteristics 被引量:5
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作者 谭美莲 校凝凝 +2 位作者 严明芳 陈金风 严兴初 《Agricultural Science & Technology》 CAS 2010年第4期39-43,共5页
[Objective]The aim was to study the effects of sunflower artificial aging on seed vigor and physiological characteristics.[Method] The varieties of seed germination capacity,vigor of germination,germination index,vigo... [Objective]The aim was to study the effects of sunflower artificial aging on seed vigor and physiological characteristics.[Method] The varieties of seed germination capacity,vigor of germination,germination index,vigor index,peroxidase(POD) activity,superoxide dismutase(SOD) activity and malondialdehyde(MDA) content for four sunflower germplasms such as'SunM20','Deep Purple Minle','Da San Kui Hua 4'and'Ji Kui 24'were studied under high temperature and high humidity conditions(100% RH,45 ℃) for different days(0 d,2 d,4 d,6 d,8 d,10 d).[Result]The result showed that the germination capacity,vigor of germination,germination index,vigor index,POD activity,SOD activity declined gradually with the increase of seed aging days,whereas MDA content enhanced by degrees;The diggerences of resistance to artifical aging existed among the four accessions,'SunM20'was the most resistant one,and exhibited the strongest seed vigor,highest activities of two protective enzyme(POD,SOD) and lowest content of MDA at the uniform condition,moreover,the seed vigor and protective enzyme activities of'SunM20'changed slowest among the four materials during the aging process.[Conclusion]The distinct reduction of POD,SOD activities maybe the main reasons for the decrease of sunflower seed vigor at the artificial aging,and the gradual accumulation of a few MDA accelerated seeds aging. 展开更多
关键词 Sunflower germplasm Artificial aging Seed vigor The resistance to aging Physiological characteristics
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