The length-weight relationship and allometric growth patterns of hatchery-reared Chinese loach, Paramisgurnus dabryanus(Dabry de Thiersant, 1872), were determined from hatching to 60 days after hatching. A best power ...The length-weight relationship and allometric growth patterns of hatchery-reared Chinese loach, Paramisgurnus dabryanus(Dabry de Thiersant, 1872), were determined from hatching to 60 days after hatching. A best power model was estimated for the length-weight relationship during the early life stages. Positive allometric growth for the head segment, trunk length, tail length and eye diameter was also found in the early life stages, while body depth, tail depth, tail fi n length, pectoral fi n length and barbel length displayed a negative coeffi cient. During the subsequent early developmental stage, the growth coeffi cients showed a clear and common tendency towards isometry for all measured body ratios. The allometric growth changes in Chinese loach during the early stage are possibly the result of selective organogenesis directed towards survival priorities.展开更多
We investigated the allelopathic properties of Alexandrium tamarense(Laboar) Balech on the growth of Prorocentrum donghaiense Lu,Chattonella marina(Subrahmanyan) Hara et Chihara and Heterosigma akashiwo(Hada) Hada in ...We investigated the allelopathic properties of Alexandrium tamarense(Laboar) Balech on the growth of Prorocentrum donghaiense Lu,Chattonella marina(Subrahmanyan) Hara et Chihara and Heterosigma akashiwo(Hada) Hada in a laboratory experiment.We examined the growth of A.tamarense,C.marina,P.donghaiense and H.Akashiwo in co-cultures and the effect of filtrates from A.tamarense cultures in various growth phases,on the three harmful algal bloom(HAB)-forming algae.In co-cultures with A.tamarense,both C.marina and H.akashiwo were dramatically suppressed at high cell densities;in contrast,the growth of P.donghaiense varied in different inoculative ratios of A.tamarense and P.donghaiense.When the ratio was 1:1(P.donghaiense:A.tamarense),growth of P.donghaiense was inhibited considerably,while the growth of P.donghaiense was almost the same as that of the control when the ratio was 9:1.The growth difference of P.donghaiense,C.marina and H.akashiwo when co-cultured with A.tamarense indicated that the allelopathic effect may be one of the important factors in algal competition and phytoplankton succession involving A.tamarense.In addition,the filtrate from A.tamarense culture had negative impacts on these three HAB algae,and such inhibition varied with different growth phases of A.tamarense in parallel with reported values of PSP toxin content in Alexandrium cells.This implied that PSP toxin was possibly involved in allelopathy of A.tamarense.However,the rapid decomposition and inactivation of PSP toxin above pH7 weakened this possibility.Further studies on the allelochemicals responsible for the allelopathy of A.tamarense need to be carried out in future.展开更多
Proline has been proposed to be an osmoprotector and scavenger of reactive oxygen species in plants subjected to water deficit. The aim of this work was to study the effects of drought on each wheat phenological stage...Proline has been proposed to be an osmoprotector and scavenger of reactive oxygen species in plants subjected to water deficit. The aim of this work was to study the effects of drought on each wheat phenological stage （tillering, booting, heading, flowering and grain-filling） using stress parameters such as the relative water content （RWC）, membrane stability index （MSI）, lipid peroxidation through malondialdehyde levels （MDA） and determination of proline content （PRO）. The Brazilian commercial elite cultivar Triticum aestivum cv. CD 200126 was submitted to eight days of water deficit stress at each stage. The perception of stress was low at tillering and high at the final stages of growth, as verified by the reduction in the MSI and RWC. However, an increase in the MDA was clearly observed. We observed a high proline accumulation when stress was applied, although it was not sufficient to prevent damages. These results indicate that the relevant stages to evaluate the effect of water shortage during wheat plant development are booting, heading and flowering.展开更多
Alcaligenes faecalis C16 was found to have the ability to heterotrophically nitrify and aerobically denitrify. In order to further understand its nitrogen removal ability and mechanism, the growth and ammonium removal...Alcaligenes faecalis C16 was found to have the ability to heterotrophically nitrify and aerobically denitrify. In order to further understand its nitrogen removal ability and mechanism, the growth and ammonium removal response were investigated at different C/N ratios and ammonium concentrations in the medium with citrate and acetate as carbon source separately. Furthermore, experiments of nitrogen sources, production of nitrogen gas and enzyme assay were conducted. Results show that the bacterium converts NH+4-N and produces NH2 OH during the growing phase and nitrite accumulation is its distinct metabolic feature. A. faecalis C16 is able to tolerate not only high ammonium concentration but also high C/N ratio, and the ammonium tolerance is associated with carbon source and C/N ratio. The nitrogen balance under different conditions shows that approximately28%–45% of the initial ammonium is assimilated into the cells, 44%–60% is denitrified and several percent is converted to nitrification products. A. faecalis C16 cannot utilize hydroxylamine, nitrite or nitrate as the sole nitrogen source for growth. However, nitrate can be used when ammonium is simultaneously present in the medium. A possible pathway for nitrogen removal by C16 is suggested. The preliminary enzyme assay provides more evidence for this nitrogen removal pathway.展开更多
The stress,strain as well as resistivity of coal during uniaxial compression process were tested based on self-built real-time testing system of loaded coal resistivity.Furthermore,the coal resistivity regularity and ...The stress,strain as well as resistivity of coal during uniaxial compression process were tested based on self-built real-time testing system of loaded coal resistivity.Furthermore,the coal resistivity regularity and mechanism were analyzed at different stages of complete stress-strain process,which includes the two kinds of coal body with typical conductive characteristics.The results indicate that coal resistivity with different conductive characteristics has different change rules in complete stress-strain process.It is mainly represented at the densification and flexibility phases before dilatation occurs.The variation of resistivity can be divided into two kinds,named down and up.Dilatation of coal samples occurred between 66%σ_(max) and 87%σ_(max).Because of dilatation,coal resistivity involves sudden change.The overall representation is shifting from reducing into improving or from slow improving into accelerated improving.Thus,coal resistivity always shows an increasing tendency at the plastic stage.After peak stress,coal body enters into failure stage.The expanding and communicating of macro fracture causes further improvement of coal resistivity.The maximum value of resistivity rangeability named λ reached 3.49.Through making real-time monitoring on coal resistivity,variation rules of resistivity can be deemed as precursory information so as to reflect the dilatation and sudden change before coal body reaches buckling failure,which can provide a new technological means for forecasting the dynamic disaster of coal petrography.展开更多
Unlike most brood parasites, several species of cowbird (Molothrus) are generalists that parasitize multiple host species across their range and within the same communities; likewise, there is little evidence that ind...Unlike most brood parasites, several species of cowbird (Molothrus) are generalists that parasitize multiple host species across their range and within the same communities; likewise, there is little evidence that individuals within a population specialize on host species. This situation has variously been attributed to the recency of cowbird evolution (the 'evolutionary lag' hypothesis) or to hidden costs of rejection by hosts (the 'equilibrium' hypothesis). Both hypotheses have some support as cowbirds are indeed a relatively young clade compared with more specialized cuckoos and cowbirds are capable of sophisticated behaviors such as retaliation against rejection ('mafia' behaviors) that would select for acceptance of cowbird eggs. Nevertheless, many species in the Americas have evolved specialized defenses against cowbird parasitism, almost all of which live in more open habitats (e. g., grasslands, shrublands, riparian strips), which indicates that coevolutionary processes can operate in ways that select for host defenses in spite of cowbird counterdefenses. We propose that the structure of landscapes in North America may explain why forest-nesting birds lack defenses against parasitism and reinforce the long-term maintenance of generalized brood parasitism in cowbirds. Because cowbirds require open habitats in which to feed, they are rare or absent in large forest tracts, which dominate much of the forest cover of the Americas. These tracts act as 'source habitats' that produce surplus young that recolonize populations in smaller, fragmented forest patches in which rates of both cowbird parasitism and nest predation are very high ('sink' habitats). Evolution of antiparasite adaptations would be very slow in this situation because most hosts are produced in areas where there is little or no cowbird parasitism. In addition, the interplay of host breeding dispersal, source-sink metapopulation dynamics, and fragmented forest habitat could further deter the evolution of host defenses against parasitism. Therefore, as long as large forest tracts remain widespread in North America, most forest birds will likely continue to lack defenses against cowbird parasitism, guaranteeing a steady supply of na ve hosts in forest habitats, even in fragmented landscapes. This situation will, in turn, favor host generalist cowbirds that actively avoid more open habitats in favor of parasitizing forest bird communities. These forest communities may also act as source populations for cowbirds, which might pump surplus generalist cowbirds into more open habitats further slowing the coevolutionary process. As long as large forest tracts are a common part of the landscape, generalist parasitism may persist indefinitely.展开更多
DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar specie...DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar species. Pleuronichthys japonicus has never been found previously in China. However, in this study, we identified both species using DNA barcoding （cytochrome c oxidase subunit I （COI））, the mtDNA control region and cytochrome b. The results reveal that： l） intraspecific variation in the DNA barcode is much less than interspecific variation; 2） the two morphologically similar species were placed into separate clades distinguishable by high bootstrap values; 3） COI barcodes are more powerful for identifying the two species than the other two mtDNA fragments.展开更多
基金Supported by the National Key Technology Research and Development Program of China(Nos.2012BAD25B08,2012BAD25B00)
文摘The length-weight relationship and allometric growth patterns of hatchery-reared Chinese loach, Paramisgurnus dabryanus(Dabry de Thiersant, 1872), were determined from hatching to 60 days after hatching. A best power model was estimated for the length-weight relationship during the early life stages. Positive allometric growth for the head segment, trunk length, tail length and eye diameter was also found in the early life stages, while body depth, tail depth, tail fi n length, pectoral fi n length and barbel length displayed a negative coeffi cient. During the subsequent early developmental stage, the growth coeffi cients showed a clear and common tendency towards isometry for all measured body ratios. The allometric growth changes in Chinese loach during the early stage are possibly the result of selective organogenesis directed towards survival priorities.
基金Supported by the NSFC-Guangdong Province Association Foundation(No.U0733006)the National Natural Science Foundation of China(No.30970502,40976065)the National Basic Research Program of China(973 Program)(No.2010CB428702)
文摘We investigated the allelopathic properties of Alexandrium tamarense(Laboar) Balech on the growth of Prorocentrum donghaiense Lu,Chattonella marina(Subrahmanyan) Hara et Chihara and Heterosigma akashiwo(Hada) Hada in a laboratory experiment.We examined the growth of A.tamarense,C.marina,P.donghaiense and H.Akashiwo in co-cultures and the effect of filtrates from A.tamarense cultures in various growth phases,on the three harmful algal bloom(HAB)-forming algae.In co-cultures with A.tamarense,both C.marina and H.akashiwo were dramatically suppressed at high cell densities;in contrast,the growth of P.donghaiense varied in different inoculative ratios of A.tamarense and P.donghaiense.When the ratio was 1:1(P.donghaiense:A.tamarense),growth of P.donghaiense was inhibited considerably,while the growth of P.donghaiense was almost the same as that of the control when the ratio was 9:1.The growth difference of P.donghaiense,C.marina and H.akashiwo when co-cultured with A.tamarense indicated that the allelopathic effect may be one of the important factors in algal competition and phytoplankton succession involving A.tamarense.In addition,the filtrate from A.tamarense culture had negative impacts on these three HAB algae,and such inhibition varied with different growth phases of A.tamarense in parallel with reported values of PSP toxin content in Alexandrium cells.This implied that PSP toxin was possibly involved in allelopathy of A.tamarense.However,the rapid decomposition and inactivation of PSP toxin above pH7 weakened this possibility.Further studies on the allelochemicals responsible for the allelopathy of A.tamarense need to be carried out in future.
文摘Proline has been proposed to be an osmoprotector and scavenger of reactive oxygen species in plants subjected to water deficit. The aim of this work was to study the effects of drought on each wheat phenological stage （tillering, booting, heading, flowering and grain-filling） using stress parameters such as the relative water content （RWC）, membrane stability index （MSI）, lipid peroxidation through malondialdehyde levels （MDA） and determination of proline content （PRO）. The Brazilian commercial elite cultivar Triticum aestivum cv. CD 200126 was submitted to eight days of water deficit stress at each stage. The perception of stress was low at tillering and high at the final stages of growth, as verified by the reduction in the MSI and RWC. However, an increase in the MDA was clearly observed. We observed a high proline accumulation when stress was applied, although it was not sufficient to prevent damages. These results indicate that the relevant stages to evaluate the effect of water shortage during wheat plant development are booting, heading and flowering.
基金National Natural Science Foundation of China(51078252)the Science and Technology Program of Taiyuan(120233)the Natural Science Foundation of Shanxi Province(2010011016–1)
文摘Alcaligenes faecalis C16 was found to have the ability to heterotrophically nitrify and aerobically denitrify. In order to further understand its nitrogen removal ability and mechanism, the growth and ammonium removal response were investigated at different C/N ratios and ammonium concentrations in the medium with citrate and acetate as carbon source separately. Furthermore, experiments of nitrogen sources, production of nitrogen gas and enzyme assay were conducted. Results show that the bacterium converts NH+4-N and produces NH2 OH during the growing phase and nitrite accumulation is its distinct metabolic feature. A. faecalis C16 is able to tolerate not only high ammonium concentration but also high C/N ratio, and the ammonium tolerance is associated with carbon source and C/N ratio. The nitrogen balance under different conditions shows that approximately28%–45% of the initial ammonium is assimilated into the cells, 44%–60% is denitrified and several percent is converted to nitrification products. A. faecalis C16 cannot utilize hydroxylamine, nitrite or nitrate as the sole nitrogen source for growth. However, nitrate can be used when ammonium is simultaneously present in the medium. A possible pathway for nitrogen removal by C16 is suggested. The preliminary enzyme assay provides more evidence for this nitrogen removal pathway.
基金supported by the Research Project of Chinese Ministry of Education of China(No.113031A)the Basic Scientific Research Business Expenses of Central University of China (Nos.3142015001 and 3142015020)the New Century Talent Supporting Project by Education Ministry of China(No. NCET-11-0837)
文摘The stress,strain as well as resistivity of coal during uniaxial compression process were tested based on self-built real-time testing system of loaded coal resistivity.Furthermore,the coal resistivity regularity and mechanism were analyzed at different stages of complete stress-strain process,which includes the two kinds of coal body with typical conductive characteristics.The results indicate that coal resistivity with different conductive characteristics has different change rules in complete stress-strain process.It is mainly represented at the densification and flexibility phases before dilatation occurs.The variation of resistivity can be divided into two kinds,named down and up.Dilatation of coal samples occurred between 66%σ_(max) and 87%σ_(max).Because of dilatation,coal resistivity involves sudden change.The overall representation is shifting from reducing into improving or from slow improving into accelerated improving.Thus,coal resistivity always shows an increasing tendency at the plastic stage.After peak stress,coal body enters into failure stage.The expanding and communicating of macro fracture causes further improvement of coal resistivity.The maximum value of resistivity rangeability named λ reached 3.49.Through making real-time monitoring on coal resistivity,variation rules of resistivity can be deemed as precursory information so as to reflect the dilatation and sudden change before coal body reaches buckling failure,which can provide a new technological means for forecasting the dynamic disaster of coal petrography.
文摘Unlike most brood parasites, several species of cowbird (Molothrus) are generalists that parasitize multiple host species across their range and within the same communities; likewise, there is little evidence that individuals within a population specialize on host species. This situation has variously been attributed to the recency of cowbird evolution (the 'evolutionary lag' hypothesis) or to hidden costs of rejection by hosts (the 'equilibrium' hypothesis). Both hypotheses have some support as cowbirds are indeed a relatively young clade compared with more specialized cuckoos and cowbirds are capable of sophisticated behaviors such as retaliation against rejection ('mafia' behaviors) that would select for acceptance of cowbird eggs. Nevertheless, many species in the Americas have evolved specialized defenses against cowbird parasitism, almost all of which live in more open habitats (e. g., grasslands, shrublands, riparian strips), which indicates that coevolutionary processes can operate in ways that select for host defenses in spite of cowbird counterdefenses. We propose that the structure of landscapes in North America may explain why forest-nesting birds lack defenses against parasitism and reinforce the long-term maintenance of generalized brood parasitism in cowbirds. Because cowbirds require open habitats in which to feed, they are rare or absent in large forest tracts, which dominate much of the forest cover of the Americas. These tracts act as 'source habitats' that produce surplus young that recolonize populations in smaller, fragmented forest patches in which rates of both cowbird parasitism and nest predation are very high ('sink' habitats). Evolution of antiparasite adaptations would be very slow in this situation because most hosts are produced in areas where there is little or no cowbird parasitism. In addition, the interplay of host breeding dispersal, source-sink metapopulation dynamics, and fragmented forest habitat could further deter the evolution of host defenses against parasitism. Therefore, as long as large forest tracts remain widespread in North America, most forest birds will likely continue to lack defenses against cowbird parasitism, guaranteeing a steady supply of na ve hosts in forest habitats, even in fragmented landscapes. This situation will, in turn, favor host generalist cowbirds that actively avoid more open habitats in favor of parasitizing forest bird communities. These forest communities may also act as source populations for cowbirds, which might pump surplus generalist cowbirds into more open habitats further slowing the coevolutionary process. As long as large forest tracts are a common part of the landscape, generalist parasitism may persist indefinitely.
基金Supported by the Special Fund for Agro-scientific Research in the Public Interest (No. 201003068)Special Key Program of Zhejiang Provincial Department of Science and Technology (No. 2008C12011)
文摘DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar species. Pleuronichthys japonicus has never been found previously in China. However, in this study, we identified both species using DNA barcoding （cytochrome c oxidase subunit I （COI））, the mtDNA control region and cytochrome b. The results reveal that： l） intraspecific variation in the DNA barcode is much less than interspecific variation; 2） the two morphologically similar species were placed into separate clades distinguishable by high bootstrap values; 3） COI barcodes are more powerful for identifying the two species than the other two mtDNA fragments.
