1,3:2,4-di-p-methylbenzylidene-D-sorbitol(MDBS)is known to be an efficient sorbitol derivative gelator.Two new sorbitol derivative gelators were designed and synthesized in contrast to MDBS in order to study the gel p...1,3:2,4-di-p-methylbenzylidene-D-sorbitol(MDBS)is known to be an efficient sorbitol derivative gelator.Two new sorbitol derivative gelators were designed and synthesized in contrast to MDBS in order to study the gel properties of gelators with different structures.Their gelation behavior to 30 solvents was investigated.It was found that the gelation behavior was related to the molecular structure of gelators.Compared with MDBS,the gelator with more hydroxyl in the molecular structure could gel water and that with more aromatic ring could gel aromatic solvent.The fibrous and three-dimensional network of the gels was obtained by scanning electron microscopy(SEM).Ultraviolet-visible(UV-Vis)spectroscopy revealed thatπ-πinteraction was one of the main driving forces for the formation of gels.Theπ-πstacking of gelation increases with the number of aromatic rings in the molecular structure of gelator.Fourier transform infrared(FT-IR)spectroscopy revealed that the hydrogen bonding was also the main driving force for the formation of gels.The layered structure of the gels was studied by X-ray diffraction(XRD).展开更多
文摘目的：探讨microRNA－21（ miR－21）反义寡核苷酸对肝星状细胞胶原合成的影响。方法肝星状细胞分离和培养，利用脂质体2000将miR－21反义寡核苷酸转染活化的肝星状细胞中，转染48 h后，收集肝星状细胞，采用实时RT－PCR法和Western blotting分别检测细胞miR－21表达和Ⅰ、Ⅲ型胶原蛋白表达；MTT法检测转染后细胞增殖活性。结果与无关反义寡核苷酸组相比，miR－21反义寡核苷酸转染后miR－21表达较无关对照组下调约76％（ P ＜0．01）；细胞增殖活性降低（26±3）％（ P ＜0．01）；Ⅰ、Ⅲ型胶原蛋白分别降低（61±7）％和（48±6）％（均P ＜0．01）。结论 miR－21反义寡核苷酸能显著下调miR－21的表达，抑制肝星状细胞增殖及胶原的合成。
基金Supported by National Natural Science Foundation of China(No.21276188)
文摘1,3:2,4-di-p-methylbenzylidene-D-sorbitol(MDBS)is known to be an efficient sorbitol derivative gelator.Two new sorbitol derivative gelators were designed and synthesized in contrast to MDBS in order to study the gel properties of gelators with different structures.Their gelation behavior to 30 solvents was investigated.It was found that the gelation behavior was related to the molecular structure of gelators.Compared with MDBS,the gelator with more hydroxyl in the molecular structure could gel water and that with more aromatic ring could gel aromatic solvent.The fibrous and three-dimensional network of the gels was obtained by scanning electron microscopy(SEM).Ultraviolet-visible(UV-Vis)spectroscopy revealed thatπ-πinteraction was one of the main driving forces for the formation of gels.Theπ-πstacking of gelation increases with the number of aromatic rings in the molecular structure of gelator.Fourier transform infrared(FT-IR)spectroscopy revealed that the hydrogen bonding was also the main driving force for the formation of gels.The layered structure of the gels was studied by X-ray diffraction(XRD).
