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微结构传感器的激光制造技术研究进展 被引量:4
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作者 陈锐 王锦成 +5 位作者 章文卓 计东生 朱鑫宁 罗涛 王菁 周伟 《光电工程》 CAS CSCD 北大核心 2023年第3期100-115,共16页
微结构传感器是具有以微尺度结构作为敏感单元,能将外界物理、化学、生物信号转化为电信号的传感器,已广泛应用于智能机器人、健康监控、虚拟电子等领域。目前,微结构传感器的制造方法主要有激光制造技术、MEMS技术和3D打印技术等。激... 微结构传感器是具有以微尺度结构作为敏感单元,能将外界物理、化学、生物信号转化为电信号的传感器,已广泛应用于智能机器人、健康监控、虚拟电子等领域。目前,微结构传感器的制造方法主要有激光制造技术、MEMS技术和3D打印技术等。激光制造技术是将高能光子束聚焦到被加工物上,使激光与物质相互作用的一种绿色加工方法,主要包括激光烧蚀、激光直写、激光诱导和激光-倒模复合加工等,具有非接触式加工、无掩膜版、可定制化制造等优势,通过优化激光加工工艺参数,可以实现不同尺寸和形状微结构的高效低成本制造。本文对微结构的类型、功能及制造技术进行了概述,同时对激光制造技术制备的微结构传感器进行了归纳分类,详细分析了生物电传感器、温度传感器以及压力传感器的制造技术及应用,最后对微结构传感器激光制造技术的发展趋势进行了总结与展望。 展开更多
关键词 激光制造 微结构 生物电传感器 温度传感器 压力传感器
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The biofilm characteristics and enhanced performance of a marine microbial-electrolysis-cell-based biosensor under positive anodic potential
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作者 CAO Yuanyuan ZHANG Chaoqun +2 位作者 LIU Xiang CHENG Liang YANG Yang 《土木与环境工程学报(中英文)》 CSCD 北大核心 2024年第6期221-230,共10页
Microbial fuel cells have already been used as biosensors to monitor assimilable organic carbon(AOC).However,their signal production from AOC is known to be completely suppressed by dissoved oxygen(DO).In this study,t... Microbial fuel cells have already been used as biosensors to monitor assimilable organic carbon(AOC).However,their signal production from AOC is known to be completely suppressed by dissoved oxygen(DO).In this study,two identical microbial electrolysis cell(MEC)based biosensors were inoculated with marine sediment and operated at two different anodic potentials,namely-300 mV and+250 mV relative to Ag/AgCl.The MEC biosensor operated under positive anodic potential conditions had electrochemically active microbial communities on the anode,including members of the Shewanellaceae,Pseudoalteromonadaceae,and Clostridiaceae families.However,the strictly anaerobic members of the Desulfuromonadaceae,Desulfobulbaceae and Desulfobacteraceae families were found only in the negative anodic potential MEC biosensor.The positive anodic potential MEC biosensor showed several other advantages as well,such as faster start-up,significantly higher maximum current production,fivefold improvement in the AOC detection limit,and tolerance of low dissolved oxygen,compared to those obtained from the negative anodic potential MEC biosensor.The developed positive anodic potential MEC biosensor can thus be used as a real-time and inexpensive detector of AOC concentrations in high saline and low DO seawater. 展开更多
关键词 BIOSENSOR microbial fuel cell anodic potential marine biofilm assimilable organic carbon
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Mushroom Pulp Tissue-Based Membrane-Ferrocene-Modified L-Tyrosine Biosensor
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作者 马全红 邓家祺 《Journal of Southeast University(English Edition)》 EI CAS 2000年第1期106-110,共5页
A new approach for assembling amperometric mushroom pulp tissue based membrane electrode for determination of L tyrosine analysis is proposed. Ferrocene is used as a mediator of electron transfer between tyrosinase ... A new approach for assembling amperometric mushroom pulp tissue based membrane electrode for determination of L tyrosine analysis is proposed. Ferrocene is used as a mediator of electron transfer between tyrosinase in mushroom tissue and a graphite electrode. The optimal operation conditions are studied. The linear response range of the biosensor is 2 0×10 -4 to 4 5×10 -3 mol·L -1 with response time of less than 5 min and lifetime of at least 30 d. The biosensor can be applied to practical sample analysis. 展开更多
关键词 BIOSENSOR tissue based membrane electrode modified electrode FERROCENE L tyrosine
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Study on Piezoelectric Immunosensor for the Detection of H9-subtype Avian Influenza Virus 被引量:2
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作者 詹爱军 胡云发 +3 位作者 王新卫 刘靖清 卞红春 陈枝楠 《Agricultural Science & Technology》 CAS 2011年第10期1517-1520,共4页
[Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus(AIV).[Method] The immunosensor chip was constructed by self-assembling mercaptopmpionic acid(MPA) to be m... [Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus(AIV).[Method] The immunosensor chip was constructed by self-assembling mercaptopmpionic acid(MPA) to be monolayer on the silver-coated electrode of quartz crystal and coupling the monoclonal antibody to H9 subtype AIV with N-ethy-N'-(3-dimethyl aminopropyl)carbodiimide hydrochloride(EDC) and N-hydroxysuccinimide(NHS).The immunosensor to detect H9 subtype AIV was established.[Result] The results showed that the immunosensor displayed better specificity to H9 AIV and had no response to H5AIV and NDV when it was used for detection.The sensitivity test indicated the detection sensitivity for the H9 subtype AIV could reach 20-100 EID50.[Conclusion] The research provided a foundation for further research on the immunosensor for detecting AIV and it could be a new approach to detect other related viruses. 展开更多
关键词 Piezoelectric immunosensor Biological self-assembly method H9 subtype avian influenza virus
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Polyaniline-graphite composite film glucose oxidase electrode 被引量:1
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作者 周海晖 陈宏 +1 位作者 陈金华 旷亚非 《Journal of Central South University of Technology》 EI 2006年第6期653-657,共5页
A novel polyaniline-graphite composite film glucose oxidase (PGCF GOD) electrode was developed. The PGCF was synthesized by cyclic voitammetry method in 0.5 mol/L H2SO4 solution containing 1 g/L graphite powder and ... A novel polyaniline-graphite composite film glucose oxidase (PGCF GOD) electrode was developed. The PGCF was synthesized by cyclic voitammetry method in 0.5 mol/L H2SO4 solution containing 1 g/L graphite powder and 0.2 mol/L aniline. The PGCF GOD electrode was prepared by doping GOD into the composite film. The morphology of the PGCF and the response property of the PGCF GOD electrode were investigated by scanning electron microscopy and electrochemical measurement, respectively. The results show that the PGCF has a porous and netty structure and the PGCF GOD electrode has excellent response property such as high sensitivity and short response time. Influences of pH value, temperature, glucose concentration and potential on the response current of the electrode were also discussed. The sensor has a maximum steady-state current density of 357.17μA/cm2 and an apparent Michaelis-Menten constant of 16.57 mmol/L. The maximum current response of the enzyme electrode occurs under the condition ofpH 5.5, 0.8 V and 65℃. 展开更多
关键词 POLYANILINE GRAPHITE composite film glucose oxidase BIOSENSOR
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A Novel 3-D Nano-assembly Bacteria Based Biosensor for Enhanced Detection of Heavy Metal Pollutants
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作者 Mina Souiri Ibtissem Gammoudi +6 位作者 Laurence Mora Hafedh Ben Ouada Thierry Jouenne Nicole Jaffrezic-Renault Corinne Dejous Ali Othmane Anthony C. Duncan 《Journal of Environmental Science and Engineering(A)》 2012年第7期924-935,共12页
Nowadays, at a time of growing concern for sustainable development and compliance with environmental standards and legislation, the detection of heavy metal contaminants in environmental matrices represents a difficul... Nowadays, at a time of growing concern for sustainable development and compliance with environmental standards and legislation, the detection of heavy metal contaminants in environmental matrices represents a difficult but important task. The current major limitation lies in the poor detection limits of the targeted pollutant's trace concentrations by the available conventional techniques. In order to elaborate a novel "living" self assembled electrochemical 3-D biosensor, the authors propose a new concept to overcome this shortcoming. The advantages of the properties of polyelectrolyte-functionalized NBs (nanobeads) are combined along with the use of non covalently strongly bound micro-organisms. The designed 3-D biosensor is all the more promising as it has showed a significantly improved sensitivity. In fact, the detection limits of the tested heavy metals (cadmium and mercury) were as low as 1.0 × 10^-12 mol.L-1 and six to seven orders of magnitude lower than those provided by conventional 2-D biosensors. Furthermore, it is potentially applicable to a wide range of bioreceptor-pollutant detection systems. 展开更多
关键词 BIOSENSOR nanobeads PEM heavy metals E. coli EIS (electrochemical impedance spectroscopy).
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Electrochemical biosensors and logic devices based on aptamers
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作者 Zuo Xiaolei Lin Meihua Fan Chunhai 《Engineering Sciences》 EI 2013年第3期76-79,共4页
Aptamers are molecular recognition elements with high specificity that are selected from deoxyribonucleic acid/ribonucleic acid (DNA/RNA) library. Compared with the traditional protein recognition elements,aptamers ha... Aptamers are molecular recognition elements with high specificity that are selected from deoxyribonucleic acid/ribonucleic acid (DNA/RNA) library. Compared with the traditional protein recognition elements,aptamers have excellent properties such as cost-effective,stable,easy for synthesis and modification. In recent years,electrochemistry plays an important role in biosensor field because of its high sensitivity,high stability, fast response and easy miniaturization. Through the combination of these two technologies and our rational design,we constructed a series of biosensors and biochips that are simple,fast,cheap and miniaturized. Firstly,we designed an adenosine triphosphate (ATP) electrochemical biosensor based on the strand displacement strategy. We can detect as low as 10 nmol/L of ATP both in pure solution and complicated cell lysates. Secondly,we creatively split the aptamers into two fragments and constructed the sandwich assay platform only based on single aptamer sequence. We successfully transferred this design on biochips with multiple micro electrodes (6×6) and accomplished multiplex detection. In the fields of biochips and biocomputers,we designed several DNA logic gates with electric (electrochemical) signal as output which paves a new way for the development of DNA computer. 展开更多
关键词 APTAMER BIOSENSOR BIOCHIP bio-computer logic device
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Research on Comparison of Gas Chromatography and Liquid Chromatogram Detecting Pesticide Residue
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作者 Meng Xinxin Yang Shulin 《International Journal of Technology Management》 2013年第2期73-75,共3页
The existing methods of detecting pesticide residue include gas chromatography, high performance liquid chromatography, gas chromatograph-mass, liquid chromatograph-mass, capillary electrophoresis, radioimmunoassay, b... The existing methods of detecting pesticide residue include gas chromatography, high performance liquid chromatography, gas chromatograph-mass, liquid chromatograph-mass, capillary electrophoresis, radioimmunoassay, biosensor and rapid detection on the spot. The paper analyzes the comparison of gas chromatography and liquid chromatogram detecting pesticide residue, for achieving the development tendency and the future goal of analyzing pesticide residue. 展开更多
关键词 pesticide residue gas chromatography liquid chromatogram
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The Process of Immobilization of ZnO Nanorods Surface with Galactose Oxidase
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作者 La Phan Phtrong Ha Ngo Van Chi Quang Tran Quang Trung 《Journal of Pharmacy and Pharmacology》 2017年第10期766-770,共5页
ZnO nanorods, with the c-axis orientation used for transparent conductors, solar cells, sensors especially the functionalized ZnO nanorods with some kinds of enzymes have been used for biosensor. In this work, we desc... ZnO nanorods, with the c-axis orientation used for transparent conductors, solar cells, sensors especially the functionalized ZnO nanorods with some kinds of enzymes have been used for biosensor. In this work, we describe the process immobilization of galactose oxidase on ZnO nanorods surface with glutaraldehyde as a cross-linker molecule to make the working electrode in electrochemical biosensor. ZnO nanorods were grown on FTO (Fluorine-doped tin oxide) substrate by solution method at low temperature. The crystalline phase and orientation of ZnO nanorods were identified using X-ray diffraction. The efficiency of the immobilization was calculated by Braford method showed that about 36% enzyme content was immobilized on ZnO nanorods surface. The working electrode based on the immobilized ZnO nanorods was tested in galactose solution by CV (cyclic voltammetry) method indicated the value of current intensity is about 0.14 μA. These results clearly demonstrate the potential of galactose sensor based on ZnO nanorod. 展开更多
关键词 ZNO solution method Bmford method galactose oxidase galactose biosensor.
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Radiation-Induced Graft Polymerization of Sodium 4-Styrene Sulfonate onto Multiwalled Carbon Nanotubes and Their Application as Electrogenerated Chemiluminescence Biosensors
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作者 Hana Ryu Hai-Doo Kwen Da-Jung Chung Seong-Ho Choi 《Journal of Chemistry and Chemical Engineering》 2012年第4期351-357,共7页
Sulfonate groups were introduced to the surfaces of multiwalled carbon nanotubes by the radiation-induced graft polymerization of sodium 4-styrene sulfonate for the use as biosensor supports. Alcohol dehydrogenase was... Sulfonate groups were introduced to the surfaces of multiwalled carbon nanotubes by the radiation-induced graft polymerization of sodium 4-styrene sulfonate for the use as biosensor supports. Alcohol dehydrogenase was immobilized onto a sulfonated nanotube-supporting electrode with tris(2,2'-bipyridyl) ruthenium(II) complex to form an electrogenerated chemilluminesce sensor of alcohol. When it was used to detect alcohol in cyclic voltammetric measurements, the sensor showed the linearity over the range of 1.0 × 10^-4 M-5.0 ×10^-2 M, with a correlation coefficient of 0.992 and a detection limit of 1.9 ×10^-6 M. In electrogenerated chemilluminesce detection, it showed linearity over 5.0 × 10^-4 M-1.0 × 10^-2 M, with a correlation coefficient of 0.986 and a detection limit of 1.0 × 10^-6 M. The sensor was demonstrated to be able to detect ethanol in commercial drinks. 展开更多
关键词 ADH-immobilized ECL biosensor MWNT supports with sulfonate group radiation-induced graft polymerization sodium 4-styrene sulfonate ruthenium complex.
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Recent advances in ionic liquid-based electrochemical biosensors 被引量:5
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作者 王晓琳 郝京诚 《Science Bulletin》 SCIE EI CAS CSCD 2016年第16期1281-1295,共15页
Ionic liquids (ILs) have been generally described as molten salts which are composed of asymmetric cations and anions. They exist in liquid state below 100 ℃. Both ILs and their composite materials have been widely... Ionic liquids (ILs) have been generally described as molten salts which are composed of asymmetric cations and anions. They exist in liquid state below 100 ℃. Both ILs and their composite materials have been widely used in various fields. Attributed to the outstanding properties including the thermal and chemical stabilities, the negligible volatility, the high ionic conductivity, the wide electrochemical window, and the easy design in the construction, ILs have been applied in electrochemical applications including the electrocatalysis, the electrosynthesis, the electrodeposition, the electrochamical devices and sensors. In addition to the application in electrochemical sensors, ILs have also been used in biosensors because of their biocompatibiciy. Here, we review the recent devel- opments for the applicaitons of ILs in electrochemical sensors and biosensors, including the corresponding properties of ILs suitable for electrochemical sensors. Electrochemical biosensors constructed by numorous composites are the emphasis in the review. 展开更多
关键词 Ionic liquids Electrochemical sensors Biosensors
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A non-labeled DNA biosensor based on light addressable potentiometric sensor modified with TiO_2 thin film 被引量:1
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作者 Xiao-lin ZONG Chun-sheng WU Xiao-ling WU Yun-feng LU Ping WANG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2009年第11期860-866,共7页
Titanium dioxide (TiO2) thin film was deposited on the surface of the light addressable potentiometric sensor (LAPS) to modify the sensor surface for the non-labeled detection of DNA molecules. To evaluate the effect ... Titanium dioxide (TiO2) thin film was deposited on the surface of the light addressable potentiometric sensor (LAPS) to modify the sensor surface for the non-labeled detection of DNA molecules. To evaluate the effect of ultraviolet (UV) treatment on the silanization level of TiO2 thin film by 3-aminopropyltriethoxysilane (APTS),fluorescein isothiocyanate (FITC) was used to label the amine group on the end of APTS immobilized onto the TiO2 thin film. We found that,with UV irradiation,the silanization level of the irradiated area of the TiO2 film was improved compared with the non-irradiated area under well-controlled conditions. This result indicates that TiO2 can act as a coating material on the biosensor surface to improve the effect and efficiency of the covalent immobilization of biomolecules on the sensor surface. The artificially synthesized probe DNA molecules were covalently linked onto the surface of TiO2 film. The hybridization of probe DNA and target DNA was monitored by the recording of I-V curves that shift along the voltage axis during the process of reaction. A significant LAPS signal can be detected at 10 μmol/L of target DNA sample. 展开更多
关键词 DNA biosensor Titanium dioxide (Ti02) thin film Light addressable potentiometric sensor (LAPS) SILANIZATION Fluorescein label Gene chip
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Preparation and potential application of functional ordered microstructures 被引量:2
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作者 SUN ZhiQiang YANG Bai 《Science China Chemistry》 SCIE EI CAS 2011年第2期275-285,共11页
With the development of science and technology,ordered microstructures with special functions have aroused intense research interest.These functional microstructures have been widely used in fields of microelectronic ... With the development of science and technology,ordered microstructures with special functions have aroused intense research interest.These functional microstructures have been widely used in fields of microelectronic devices,micro-reactors,biochemical sensors and optical devices,etc.This paper summaries our work on preparation and application of microscopic patterned surfaces with ordered microstructures,and looks into the future development of this field. 展开更多
关键词 ordered microstructures colloidal crystals DEWETTING
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Solid-state electrochemiluminescence protein biosensor with aptamer substitution strategy 被引量:2
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作者 XU Ying DONG Ping +2 位作者 ZHANG XiaoYan HE PinGang FANG YuZhi 《Science China Chemistry》 SCIE EI CAS 2011年第7期1109-1115,共7页
One solid-state electrochemiluminescence (ECL) protein biosensor based on the competing reaction and substitute reaction between protein-to-DNA aptamer and DNA-to-DNA aptamer was proposed. Additionally, the biosenso... One solid-state electrochemiluminescence (ECL) protein biosensor based on the competing reaction and substitute reaction between protein-to-DNA aptamer and DNA-to-DNA aptamer was proposed. Additionally, the biosensor was based on ECL photo-quenching effect of ferrocene (Fc) to tris(2,2-bipyridyl)ruthenium(II) (Ru(bpy)2+). It was built up by modification of Au nanoparticles (AuNPs) and Ru(bpy)32+ on one Au electrode firstly, and then self-assembly of one special double-stranded DNA (dsDNA) onto the electrode. This dsDNA was prepared by hybridization of one Fc labeled molecular beacon single-stranded DNA(ssDNA) and one anti-thrombin aptamer ssDNA. Without the target protein, this Fc-dsDNA/Ru(bpy)2+- AuNPs/Au elec- trode trigged strong ECL signal, so we called it ECL "signal on" state. When thrombin was present in the sensing solution, the protein reacted with its aptamer from the Fc-dsDNA/Ru(bpy)3^2+-AuNPs/Au electrode. Then the left molecular beacon ssDNA on the electrode recovered to its normal stem-loop structure and consequently its Fc labeler was close enough to the electrode surface to quench the ECL signal from Ru(bpy)3^2+. It was in ECL "signal off" state. We measured the decrease in ECL intensity to sense the target protein. This was one endeavour to sense protein by using un-labeling target or probe strategy, which gave higher sensitivity and selectivity due to the better combination efficiency of protein and the un-labeled aptamer. 6.25 fmo/L thrombin was detected out, 展开更多
关键词 electrochemiluminescence biosensor APTAMER tris(2 2'-bipyridyl)ruthenium(II)
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Recent advances in flexible and stretchable electronics, sensors and power sources 被引量:10
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作者 TOK Jeffrey B.-H. 《Science China Chemistry》 SCIE EI CAS 2012年第5期718-725,共8页
There has been ongoing keen interest to mold electronic devices into desired shapes and be laid on desired configurable surfaces. In specific, the ability to design materials that can bend, twist, compress and stretch... There has been ongoing keen interest to mold electronic devices into desired shapes and be laid on desired configurable surfaces. In specific, the ability to design materials that can bend, twist, compress and stretch repeatedly, while still able to maintain its full capability as conductors or electrodes, has led to numerous efforts to develop flexible and stretchable (bio)devices that are both technologically challenging and environmentally friendly (e.g. biodegradable). In this review, we highlight several recent significant results that have made impacts toward the field of flexible and stretchable electronics, sensors and power sources. 展开更多
关键词 organic transistors flexible electronics stretchable electronics electronic skin SENSOR
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Manipulation of gold coated microspheres using electrorotation 被引量:2
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作者 JIANG HongYuan REN YuKun +2 位作者 HAN XiaoJun TAO Ye LI ShanShan 《Science China(Technological Sciences)》 SCIE EI CAS 2011年第3期643-649,共7页
The electrorotation of microspheres coated with conductive surface is a novel and important technology for label-free biosensors. Using the electroless plating approach, the polystyrene microspheres with 15 μm and 25... The electrorotation of microspheres coated with conductive surface is a novel and important technology for label-free biosensors. Using the electroless plating approach, the polystyrene microspheres with 15 μm and 25 μm in diameters were coated with 50 nm gold layer in thickness. The electrorotation experiments on those gold coated polystyrene microspheres (GCPMs) were carried out. The results showed that they rotated in the opposite direction of the electric field in a low frequency range (100-100 kHz), and the maximum rotation speed was higher than that of uncoated microspheres. Based on the theory of traveling wave electroosmosis(TWEO) and induced charge electroosmosis (ICEO), the electrorotation of GCPMs was quantitively analyzed and confirmed by observing the fluid flow around GCPM. The equations describing the electroration speed of GCPMs were proposed, which are consistent with the experiment results. 展开更多
关键词 ELECTROROTATION gold coated microspheres TWEO ICEO
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Organic transistor for bioelectronic applications 被引量:2
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作者 Hongguang Shen Chong-An Di Daoben Zhu 《Science China Chemistry》 SCIE EI CAS CSCD 2017年第4期437-449,共13页
Organic field-effect transistors(OFETs) are recently considered to be attractive candidate for bioelectronic applications owing to their prominent biocompatibility,intrinsical flexibility,and potentially low cost asso... Organic field-effect transistors(OFETs) are recently considered to be attractive candidate for bioelectronic applications owing to their prominent biocompatibility,intrinsical flexibility,and potentially low cost associated with their solution processibility.Over the last few years,bioelectronic-application-motivated OFETs have attracted increasing attention towards next generation of biosensors,healthcare elements and artificial neural interfaces.This mini review highlights the basic principles and recent progress in OFET based bioelectronics devices.The key strategies and the forecast perspectives of this research field are also briefly summarized. 展开更多
关键词 organic semiconductor OFET OECT BIOSENSOR BIOELECTRONICS
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Metal chelate affinity to immobilize horseradish peroxidase on functionalized agarose/CNTs composites for the detection of catechol 被引量:1
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作者 TU XinMan LUO ShengLian +3 位作者 LUO XuBiao ZHAO YingJie FENG Li LI JingHong 《Science China Chemistry》 SCIE EI CAS 2011年第8期1319-1326,共8页
The paper reports a novel amperometric biosensor for catechol based on immobilization of a highly sensitive horseradish peroxidase by affinity interactions on metal chelate-functionalized agarose/carbon nanotubes comp... The paper reports a novel amperometric biosensor for catechol based on immobilization of a highly sensitive horseradish peroxidase by affinity interactions on metal chelate-functionalized agarose/carbon nanotubes composites. Metal chelate affinity takes advantage of the affinity of Ni2+ ions to bind strongly and reversibly to histidine or cysteine tails found on the surface of the horseradish peroxidase. Thus, enzymes with such residues in their molecules can be easily attached to functionalized aga- rose/carbon nanotubes composites support containing a nickel chelate. Linear sweep voltammograms and amperometry are used to study the proposed electrochemical biosensor. Catechol is determined by direct reduction of biocatalytically liberated quinone species at -0.05 V (vs. SCE). The effect ofpH, applied electrode potential and the concentration of H2O2 on the sensitivity of the biosensor has been investigated. The performance of the proposed biosensor is tested using four different phenolic compounds, showing very high sensitivity, in particular, the linearity of cateehol is observed from 2.0 × 10-8 to 1.05×10-5 M with a detection limit of 5.0×10-9 M. 展开更多
关键词 amperometric biosensor carbon nanotubes metal chelation AGAROSE CATECHOL
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Assembly of layer-by-layer films of superoxide dismutase and gold nanorods:A third generation biosensor for superoxide anion 被引量:1
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作者 WANG MengDong HAN YiTao +4 位作者 LIU XingXing NIE Zhou DENG ChunYan GUO ManLi YAO ShouZhuo 《Science China Chemistry》 SCIE EI CAS 2011年第8期1284-1291,共8页
Based on the layer-by-layer self-assembly of positively charged cetyltrimethylammonium bromide (CTAB) wrapped gold na- norods (AuNRs) and negatively charged superoxide dismutase (SOD) from their aqueous solution... Based on the layer-by-layer self-assembly of positively charged cetyltrimethylammonium bromide (CTAB) wrapped gold na- norods (AuNRs) and negatively charged superoxide dismutase (SOD) from their aqueous solutions on cysteine modified gold electrode (Cys/Au), a third generation electrochemical biosensor ((SOD/AuNRs)2/Cys/Au) for superoxide anion (02"-) was developed. The two layers assembly of SOD/AuNRs can significantly enhance the direct electron transfer between SOD and the electrode. The functional enzymatic activities of the SOD offer an electrochemical approach to the determination of 02"-. In the reductive regions, the proposed sensor exhibits excellent analytical performances, such as wide linear range (200 nM to 0.2 mM O2-), low detection limit (100 nM O2-), high sensitivity (22.11 nA cm-2 μM-1), short response time (less than 5 s), good stability and reproducibility, while no obvious interferences are caused by commonly met interfering species including hydrogen peroxide (H202), uric acid (UA) and ascorbic acid (AA). 展开更多
关键词 superoxide dismutase (SOD) gold nanorods (AuNRs) layer-by-layer self-assembly direct electrochemistry superoxideanion sensor
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Celestine blue as a new indicator in electrochemical DNA biosensors
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作者 Ezat Hamidi-Asl Jahan Bakhsh Raoof +1 位作者 Abdolraoof Samadi Meibodi Zahra Haghnavaz Bazgir 《Science China Chemistry》 SCIE EI CAS CSCD 2016年第1期128-134,共7页
Celestine blue(CB)was introduced as a new electroactive indicator in DNA biosensors.The interaction of CB with DNA was investigated by electrochemical and spectroscopic methods.The effect of buffer kind and p H on the... Celestine blue(CB)was introduced as a new electroactive indicator in DNA biosensors.The interaction of CB with DNA was investigated by electrochemical and spectroscopic methods.The effect of buffer kind and p H on the electrochemical behavior of CB was studied.The peak currents of CB were linearly related to DNA concentration in the range of 5.0×10^(-9) to 1.0×10^(-7)mol/L.The detection limit of this approach was 4.76×10^(-10) mol/L.Based on spectrometry data a hypochromic effect was observed in UV-Vis spectra of CB with increasing DNA concentration.The results illustrate the possible interaction mode between CB and DNA is electrostatic binding. 展开更多
关键词 celestine blue differential pulse voltammetry DNA biosensor electroactive indicator
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