雪松疫霉根腐病菌(Phytophthora lateralis Tucker et Milbrath)是我国进境植物检疫性有害生物,可引起寄主植物严重的根腐病。鉴于该病原菌潜在的危险性,本文就其分布、寄主范围、传播方式、危害症状、形态特征以及检疫鉴定方法等进行...雪松疫霉根腐病菌(Phytophthora lateralis Tucker et Milbrath)是我国进境植物检疫性有害生物,可引起寄主植物严重的根腐病。鉴于该病原菌潜在的危险性,本文就其分布、寄主范围、传播方式、危害症状、形态特征以及检疫鉴定方法等进行了综述,供检疫鉴定参考。展开更多
The internal transcribed spacer （ITS） region （ITS1, ITS2 and 5.8S rDNA） of the nuclear ribosomal DNA （nrDNA） was amplified via the PCR method in seventeen different isolates of Phytophthora sojae using the commo...The internal transcribed spacer （ITS） region （ITS1, ITS2 and 5.8S rDNA） of the nuclear ribosomal DNA （nrDNA） was amplified via the PCR method in seventeen different isolates of Phytophthora sojae using the common primers of the ITS of fungi. Around 800 bp- 1,000 bp fragments were obtained based on the DL2000 marker and the sequences of the PCR products were tested. Taking isolate USA as outgroup, the phylogenetic tree was constructed by means of maximum parsimony analysis, and the genetic evolution among isolates was analyzed. The results showed that there is a great difference between the base constitution of ITS 1 and ITS2 among various isolates. The seventeen isolates are classified into three groups, and the isolates from the same region belong to the same group, which shows the variation in geography.展开更多
基金This work was supported by National Natural Science Fundation of China (No.30400285, 30671317), Postdoctoral Grant from Ag-riculture Sciences Academy of Heilongjiang Province (No. LRB06-010), China Postdoctoral Grant, Item for Teachers from Heilongjiang University (No. 140022), Young People’s Science Fund of Heilongjiang Province (No. QC06C012), 973(No. 2004CB117203-4), the Opening Fund of Key Opening Laboratory of Physiology and Ecology of Crop in Cold Terra of Agriculture Ministry "the Cloning and Mapping of cDNA Sequence from Related Gene Resistant to Phytophthora sojae and International Tech-nology Cooperation Item (No. 2005DFA30340).
文摘The internal transcribed spacer （ITS） region （ITS1, ITS2 and 5.8S rDNA） of the nuclear ribosomal DNA （nrDNA） was amplified via the PCR method in seventeen different isolates of Phytophthora sojae using the common primers of the ITS of fungi. Around 800 bp- 1,000 bp fragments were obtained based on the DL2000 marker and the sequences of the PCR products were tested. Taking isolate USA as outgroup, the phylogenetic tree was constructed by means of maximum parsimony analysis, and the genetic evolution among isolates was analyzed. The results showed that there is a great difference between the base constitution of ITS 1 and ITS2 among various isolates. The seventeen isolates are classified into three groups, and the isolates from the same region belong to the same group, which shows the variation in geography.
