The present study evaluated the ef fects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm mot...The present study evaluated the ef fects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx), glutathione reductase(Gr), and lipid peroxidation(measured via malondialdehyde(MDA) content) were determined after the milt was stored at 4°C for 12 h, cryopreserved without cryoprotectant in 12% propylene glycol(PG), cryopreserved in 12% PG+0.1 mol/L trehalose, or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation(only sperm cells were cryopreserved). After chilled storage or cryopreservation, the SOD, CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However, the addition of trehalose into the cryoprotectant could signifi cantly improve the postthaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had signifi cant eff ects on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod.展开更多
Correlations among free radicals, apparent activation energy, and functional groups during lowtemperature oxidation of Jurassic coal in Northern Shaanxi were investigated by examining three coal samples collected from...Correlations among free radicals, apparent activation energy, and functional groups during lowtemperature oxidation of Jurassic coal in Northern Shaanxi were investigated by examining three coal samples collected from the Ningtiaota, Jianxin, and Shigetai coal mines. Free radical concentrations at less than 120 ℃ were investigated by electron spin resonance experiments while the thermogravimetric experiments were conducted to analyze apparent activation energies. In addition, Fourier transform infrared spectroscopy was employed to study the spectrum of functional groups generated in coal. The results indicated that, in decreasing order, the apparent activation energies were Shigetai 〉Jianxin 〉 Ningtiaota, indicating that, from 50 to 120 ℃, the Ningtiaota coal sample most easily absorbed and reacted with oxygen while the most resistant was the Shigetai coal sample. Free radical concentrations and line heights increased with increased temperature, and the line width and Lande factor showed irregular fluctuations. Functional group variations were different among these coals, and the phenol and alcohol-associated OHs, carboxyls, and aromatic ring double bonds might have had a major impact on free radical concentrations. These results were meaningful for better consideration and management of coal oxidation at low temperatures.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.31072212,41076100)the CAS Scientific and Technological Innovation Program for Cross and Cooperative Team,Marine Economy Innovative Development Project(No.12PYY001SF08)the National Key Basic Program of Science and Technology Platforms of Aquaculture Stock Resources,Shandong Technology Development Project(No.2013GHY11509)
文摘The present study evaluated the ef fects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx), glutathione reductase(Gr), and lipid peroxidation(measured via malondialdehyde(MDA) content) were determined after the milt was stored at 4°C for 12 h, cryopreserved without cryoprotectant in 12% propylene glycol(PG), cryopreserved in 12% PG+0.1 mol/L trehalose, or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation(only sperm cells were cryopreserved). After chilled storage or cryopreservation, the SOD, CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However, the addition of trehalose into the cryoprotectant could signifi cantly improve the postthaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had signifi cant eff ects on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod.
基金supported by the Key Projects of the National Natural Science Foundation of China (Nos. 51504187, 51774233, and 51704226)Shaanxi Province Industrial Science and Technology Research Project (No. 2016GY-192)the China Postdoctoral Science Foundation (No. 2016-M-590963)
文摘Correlations among free radicals, apparent activation energy, and functional groups during lowtemperature oxidation of Jurassic coal in Northern Shaanxi were investigated by examining three coal samples collected from the Ningtiaota, Jianxin, and Shigetai coal mines. Free radical concentrations at less than 120 ℃ were investigated by electron spin resonance experiments while the thermogravimetric experiments were conducted to analyze apparent activation energies. In addition, Fourier transform infrared spectroscopy was employed to study the spectrum of functional groups generated in coal. The results indicated that, in decreasing order, the apparent activation energies were Shigetai 〉Jianxin 〉 Ningtiaota, indicating that, from 50 to 120 ℃, the Ningtiaota coal sample most easily absorbed and reacted with oxygen while the most resistant was the Shigetai coal sample. Free radical concentrations and line heights increased with increased temperature, and the line width and Lande factor showed irregular fluctuations. Functional group variations were different among these coals, and the phenol and alcohol-associated OHs, carboxyls, and aromatic ring double bonds might have had a major impact on free radical concentrations. These results were meaningful for better consideration and management of coal oxidation at low temperatures.
