A new record of Pagrus caeruleostictus(Valenciennes,1830),collected from the Beibu Gulf,South China Sea in April 2013,was documented based on morphology and cytochrome oxidase I subunit(COI) gene barcoding analyses. I...A new record of Pagrus caeruleostictus(Valenciennes,1830),collected from the Beibu Gulf,South China Sea in April 2013,was documented based on morphology and cytochrome oxidase I subunit(COI) gene barcoding analyses. It can be distinguished by a combination of the following characteristics: head scaled to above eyes; cheeks with 5 or 6 rows of scales; lateral line scales 51–52; 5 rows of scales above the lateral line; 4 and 6 cuspidate teeth in front of upper and lower jaws,respectively,followed by 2 rows of blunter teeth posteriorly; gill rakers on first arch 12 to 15; D XI–XII +9–11; A III +8–9; the first two dorsal spines very short,the third to fifth extended,filamentous in the young; the first pelvic ray filamentous; silvery pink with dark blue spots on back and sides; caudal fin pinkish; other fins bluish or pinkish; the blue spots generally disappear in large specimens. The present report of P. caeruleostictus suggests that its distribution in Chinese coastal waters may be attributed to human effort,since this species is unlikely to have expanded naturally from the eastern Atlantic to the South China Sea,with no other records from the Indian or Pacific Oceans. We recommend that a precautionary approach should be adopted for the management of P. caeruleostictus.展开更多
Salinity is a serious threat to agricultural production. Potato (Solanum tuberosum) is an important food crop characterised for having low to moderate salinity tolerance. Tissue cultures may be relevant to improve s...Salinity is a serious threat to agricultural production. Potato (Solanum tuberosum) is an important food crop characterised for having low to moderate salinity tolerance. Tissue cultures may be relevant to improve salt tolerance in potato through selection of salt-tolerant cell lines and subsequent regeneration of plants. In this work, the authors used the random amplified polymorphic DNA (RAPD) markers to investigate the occurrence of genetic polymorphism in a potato calli line tolerant to 150 mM NaCI. Out of 40 primers screened, eight generated polymorphic patterns that distinguished salt-tolerant line from the control. Although the macroscopic appearance was similar in both lines, ultrastructural study revealed alterations in salt-grown cells. These showed that plastids less differentiated with a lower number of grana had more and larger starch grains than control cells. In conclusion, RAPD analysis revealed that NaCl-adapted line is a somaclonal variant and the ultrastructural study showed changes essentially at the plastids.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.31172053,41276166,31372532,31372166)the Project for Outstanding Young Teachers in Higher Education of Guangdong,China(No.Yq2013093)
文摘A new record of Pagrus caeruleostictus(Valenciennes,1830),collected from the Beibu Gulf,South China Sea in April 2013,was documented based on morphology and cytochrome oxidase I subunit(COI) gene barcoding analyses. It can be distinguished by a combination of the following characteristics: head scaled to above eyes; cheeks with 5 or 6 rows of scales; lateral line scales 51–52; 5 rows of scales above the lateral line; 4 and 6 cuspidate teeth in front of upper and lower jaws,respectively,followed by 2 rows of blunter teeth posteriorly; gill rakers on first arch 12 to 15; D XI–XII +9–11; A III +8–9; the first two dorsal spines very short,the third to fifth extended,filamentous in the young; the first pelvic ray filamentous; silvery pink with dark blue spots on back and sides; caudal fin pinkish; other fins bluish or pinkish; the blue spots generally disappear in large specimens. The present report of P. caeruleostictus suggests that its distribution in Chinese coastal waters may be attributed to human effort,since this species is unlikely to have expanded naturally from the eastern Atlantic to the South China Sea,with no other records from the Indian or Pacific Oceans. We recommend that a precautionary approach should be adopted for the management of P. caeruleostictus.
文摘Salinity is a serious threat to agricultural production. Potato (Solanum tuberosum) is an important food crop characterised for having low to moderate salinity tolerance. Tissue cultures may be relevant to improve salt tolerance in potato through selection of salt-tolerant cell lines and subsequent regeneration of plants. In this work, the authors used the random amplified polymorphic DNA (RAPD) markers to investigate the occurrence of genetic polymorphism in a potato calli line tolerant to 150 mM NaCI. Out of 40 primers screened, eight generated polymorphic patterns that distinguished salt-tolerant line from the control. Although the macroscopic appearance was similar in both lines, ultrastructural study revealed alterations in salt-grown cells. These showed that plastids less differentiated with a lower number of grana had more and larger starch grains than control cells. In conclusion, RAPD analysis revealed that NaCl-adapted line is a somaclonal variant and the ultrastructural study showed changes essentially at the plastids.