Aim:To evaluate the effect of intracytoplasmic sperm injection(ICSI)in the management of cases with a history of conventional in vitro fertilization(IVF)failure.Methods:Two groups of patients,19 with normal semen para...Aim:To evaluate the effect of intracytoplasmic sperm injection(ICSI)in the management of cases with a history of conventional in vitro fertilization(IVF)failure.Methods:Two groups of patients,19 with normal semen parameters and a history of IVF failure(metaphase Ⅱ oocytes:0-30%)and 28 with severe male factor infertility received ICSI technology during the same period.Ovarian stimulation was achieved by conventional procedure.Transvaginal ultrasound-guided oocyte collection was done 35-37 h after human chorionic gonadotrophin(hCG) injection.Only metaphase Ⅱ oocytes were selected for microinjection.Results:Fertilization was achieved with ICSI in all the patients.The fertilization rate(75.6%±2.11% vs.73.9%±19.2%),cleavage rate(85.1%±19.3% vs.82.7%±22.1%),clinical pregnancy rate per embryo transfer cycle(31.6%vs.28.6%)and implantation rate per embryo(15.3% vs 14.4%)aid not differ significantly between the two groups.Conclusion:ICSI is a valuable method for couples with a history of IVF failure.These patients may have a similar ICSI result as in severe male infertility.展开更多
Aim:To describe the reproductive outcome following intracytoplasmic sperm injection(ICSI)for male factor infertility associated with Crohn's disease and 6-mercaptopurine(6-MP) chemotherapy.Methods:The male partner...Aim:To describe the reproductive outcome following intracytoplasmic sperm injection(ICSI)for male factor infertility associated with Crohn's disease and 6-mercaptopurine(6-MP) chemotherapy.Methods:The male partner of a couple suffered from severe Crohn's disease and received a 3-month course of 6-MP for this condition.Two spontaneous conceptions were established before 6-MP,although post-chemotherapy semen analysis found the sperm concentration to be 8,000/mL.In vitro fertilization(IVF)with ICSI and embryo transfer was performed.Results:The woman underwent an uncomplicated controlled ovarian hyperstimulation sequence using a combined rec-FSH+hMG protocol,following late luteal phase pituitary downregulation.This culminated in the retrieval of 18 oocytes,11 of which were fertilized with ICSI.She later delivered a normal male infant without urogenital anomaly.Four nontransferred blastocysts were cryopreserved.Conclusion:This report describes the first successful birth after ICSI for severe oligozoospermia associated with Crohn''''s disease and 6-MP therapy.We outline salient features of Crohn's disease,6-MP pharmacology,and their relevance to human fertility.展开更多
文摘Aim:To evaluate the effect of intracytoplasmic sperm injection(ICSI)in the management of cases with a history of conventional in vitro fertilization(IVF)failure.Methods:Two groups of patients,19 with normal semen parameters and a history of IVF failure(metaphase Ⅱ oocytes:0-30%)and 28 with severe male factor infertility received ICSI technology during the same period.Ovarian stimulation was achieved by conventional procedure.Transvaginal ultrasound-guided oocyte collection was done 35-37 h after human chorionic gonadotrophin(hCG) injection.Only metaphase Ⅱ oocytes were selected for microinjection.Results:Fertilization was achieved with ICSI in all the patients.The fertilization rate(75.6%±2.11% vs.73.9%±19.2%),cleavage rate(85.1%±19.3% vs.82.7%±22.1%),clinical pregnancy rate per embryo transfer cycle(31.6%vs.28.6%)and implantation rate per embryo(15.3% vs 14.4%)aid not differ significantly between the two groups.Conclusion:ICSI is a valuable method for couples with a history of IVF failure.These patients may have a similar ICSI result as in severe male infertility.
文摘Aim:To describe the reproductive outcome following intracytoplasmic sperm injection(ICSI)for male factor infertility associated with Crohn's disease and 6-mercaptopurine(6-MP) chemotherapy.Methods:The male partner of a couple suffered from severe Crohn's disease and received a 3-month course of 6-MP for this condition.Two spontaneous conceptions were established before 6-MP,although post-chemotherapy semen analysis found the sperm concentration to be 8,000/mL.In vitro fertilization(IVF)with ICSI and embryo transfer was performed.Results:The woman underwent an uncomplicated controlled ovarian hyperstimulation sequence using a combined rec-FSH+hMG protocol,following late luteal phase pituitary downregulation.This culminated in the retrieval of 18 oocytes,11 of which were fertilized with ICSI.She later delivered a normal male infant without urogenital anomaly.Four nontransferred blastocysts were cryopreserved.Conclusion:This report describes the first successful birth after ICSI for severe oligozoospermia associated with Crohn''''s disease and 6-MP therapy.We outline salient features of Crohn's disease,6-MP pharmacology,and their relevance to human fertility.
