目的:调查临床结核菌分离株对抗痨药物依赖状况。方法:采用双向匡氏琼脂培养基分离痰结核菌和作结核菌依赖性测定。采用匡氏琼脂培养基检测结核菌耐药性。结果:依赖S11.41%(21/184),依赖R14.13%(26/184),依赖H6.52%(12/184),依赖K2.17%(...目的:调查临床结核菌分离株对抗痨药物依赖状况。方法:采用双向匡氏琼脂培养基分离痰结核菌和作结核菌依赖性测定。采用匡氏琼脂培养基检测结核菌耐药性。结果:依赖S11.41%(21/184),依赖R14.13%(26/184),依赖H6.52%(12/184),依赖K2.17%(4/184),依赖E10.87%(20/184),依赖P4.89%(9/184),依赖O5.43%(10/184),依赖Z8.15%(15/184);H R、H R S、H R E的依赖率分别为13.04%(24/184)、9.78%(18/184)、7.07%(13/184)。结论:结核杆菌对8种药物都存在不同程度的依赖现象,结核杆菌对3种药物组合均有不同程度的依赖性;对抗痨药物E、P、O、Z和抗痨药物组合H R、H R S、H R E的依赖现象国内未见报道。结核杆菌对不同药物的依赖率不同,对R依赖率最高,组合药物依赖中对H R的依赖率最高。展开更多
To study the characteristics of drug-resistant genetic mutation of rpoB on coal workers＇ pneumoconiosis complicated with L-form of Mycobacterium tuberculosis. Methods： A total of 42 clinical isolated strains of Myco...To study the characteristics of drug-resistant genetic mutation of rpoB on coal workers＇ pneumoconiosis complicated with L-form of Mycobacterium tuberculosis. Methods： A total of 42 clinical isolated strains of Mycobacterium tuberculosis L-forms were collected, including 31 drug-resistant strains. Their genomes DNA were extracted, the target genes were amplified by PCR, and the hot regions in the rpoB gene were analyzed by automated DNA sequenator. Results： No mutation of rpoB gene was identified in 11 rifampicin-sensitive strains while conformation changes were found in 31 rifampicin-resistant strains. The mutation rate was 93.55% （29/31） in resistant strains, mainly concentrated in codon 531 （51.6%, 16/31） and 526 （32.26%, 10/31）. Base substitutions happened, including 27 unit point mutation and 2 two point mutation. The mutation of codon 516 that new found wasn＇t reported by internal and overseas scholars. Conclusion： The substitution of highly conserved amino acids encoded by rpoB gene results in the molecular mechanism responsible for rifampicin resistance in Mycobacterium tuberculosis L-forms. It also proves that rpoB gene is diversiform.展开更多
文摘目的:调查临床结核菌分离株对抗痨药物依赖状况。方法:采用双向匡氏琼脂培养基分离痰结核菌和作结核菌依赖性测定。采用匡氏琼脂培养基检测结核菌耐药性。结果:依赖S11.41%(21/184),依赖R14.13%(26/184),依赖H6.52%(12/184),依赖K2.17%(4/184),依赖E10.87%(20/184),依赖P4.89%(9/184),依赖O5.43%(10/184),依赖Z8.15%(15/184);H R、H R S、H R E的依赖率分别为13.04%(24/184)、9.78%(18/184)、7.07%(13/184)。结论:结核杆菌对8种药物都存在不同程度的依赖现象,结核杆菌对3种药物组合均有不同程度的依赖性;对抗痨药物E、P、O、Z和抗痨药物组合H R、H R S、H R E的依赖现象国内未见报道。结核杆菌对不同药物的依赖率不同,对R依赖率最高,组合药物依赖中对H R的依赖率最高。
基金Supported by the Natural Science Foundation of Universities of Anhui Province (KJ2008A152)the Natural Science Foundation of the Committee of Education of Anhui Province (2005KJ238)
文摘To study the characteristics of drug-resistant genetic mutation of rpoB on coal workers＇ pneumoconiosis complicated with L-form of Mycobacterium tuberculosis. Methods： A total of 42 clinical isolated strains of Mycobacterium tuberculosis L-forms were collected, including 31 drug-resistant strains. Their genomes DNA were extracted, the target genes were amplified by PCR, and the hot regions in the rpoB gene were analyzed by automated DNA sequenator. Results： No mutation of rpoB gene was identified in 11 rifampicin-sensitive strains while conformation changes were found in 31 rifampicin-resistant strains. The mutation rate was 93.55% （29/31） in resistant strains, mainly concentrated in codon 531 （51.6%, 16/31） and 526 （32.26%, 10/31）. Base substitutions happened, including 27 unit point mutation and 2 two point mutation. The mutation of codon 516 that new found wasn＇t reported by internal and overseas scholars. Conclusion： The substitution of highly conserved amino acids encoded by rpoB gene results in the molecular mechanism responsible for rifampicin resistance in Mycobacterium tuberculosis L-forms. It also proves that rpoB gene is diversiform.
