目的:研究S-D大鼠肺缺血再灌注损伤模型中肺组织铜离子水平变化。方法:用左侧开胸夹闭肺门法构建大鼠肺缺血再灌注损伤模型,测量其肺组织中铜离子水平并与假手术组对照,并利用Western Blot研究其细胞膜铜离子转运蛋白水平、脂酰化蛋白...目的:研究S-D大鼠肺缺血再灌注损伤模型中肺组织铜离子水平变化。方法:用左侧开胸夹闭肺门法构建大鼠肺缺血再灌注损伤模型,测量其肺组织中铜离子水平并与假手术组对照,并利用Western Blot研究其细胞膜铜离子转运蛋白水平、脂酰化蛋白水平。结果:大鼠肺缺血再灌注损伤组肺组织铜离子含量明显高于对照组,SLC31A1蛋白高于对照组,ATP7B蛋白低于对照组,脂酰化蛋白水平明显升高。结论:S-D大鼠肺缺血再灌注损伤模型组织铜离子水平升高。Objective: To investigate the changes of copper level in lung tissue in S-D rats with lung ischemia-reperfusion injury. Methods: A rat model of lung ischemia-reperfusion injury was established by clamping the pulmonary hilum through left thoracotomy. The copper levels in lung tissue were measured and compared with those in the sham-operated group. Additionally, Western blot was used to investigate the levels of copper ion transport proteins and acylated proteins in the cell membrane. Results: The copper content in the lung tissue of the lung ischemia-reperfusion injury group was significantly higher than that of the control group. The level of SLC31A1 protein was higher than that of the control group, while the level of ATP7B protein was lower than that of the control group. The levels of acylated proteins were significantly increased. Conclusion: Copper level is increased in lung I/R injury model of S-D rats.展开更多
文摘目的:研究S-D大鼠肺缺血再灌注损伤模型中肺组织铜离子水平变化。方法:用左侧开胸夹闭肺门法构建大鼠肺缺血再灌注损伤模型,测量其肺组织中铜离子水平并与假手术组对照,并利用Western Blot研究其细胞膜铜离子转运蛋白水平、脂酰化蛋白水平。结果:大鼠肺缺血再灌注损伤组肺组织铜离子含量明显高于对照组,SLC31A1蛋白高于对照组,ATP7B蛋白低于对照组,脂酰化蛋白水平明显升高。结论:S-D大鼠肺缺血再灌注损伤模型组织铜离子水平升高。Objective: To investigate the changes of copper level in lung tissue in S-D rats with lung ischemia-reperfusion injury. Methods: A rat model of lung ischemia-reperfusion injury was established by clamping the pulmonary hilum through left thoracotomy. The copper levels in lung tissue were measured and compared with those in the sham-operated group. Additionally, Western blot was used to investigate the levels of copper ion transport proteins and acylated proteins in the cell membrane. Results: The copper content in the lung tissue of the lung ischemia-reperfusion injury group was significantly higher than that of the control group. The level of SLC31A1 protein was higher than that of the control group, while the level of ATP7B protein was lower than that of the control group. The levels of acylated proteins were significantly increased. Conclusion: Copper level is increased in lung I/R injury model of S-D rats.
