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湘西黄牛IGF–IR基因的群体遗传多态性及其与生长性状的关联分析 被引量:2
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作者 王兴平 罗仍卓么 +3 位作者 欧阳助姣 李峰 李娜 王京仁 《湖南农业大学学报(自然科学版)》 CAS CSCD 北大核心 2014年第4期389-394,共6页
为探讨湘西黄牛的分子遗传特征,寻找与湘西黄牛生长性状相关的分子标记,用PCR和基因测序技术检测了湘西黄牛IGF–IR基因部分序列的碱基突变,并采用PCR–RFLP方法进行了群体遗传多态性分析。结果表明:PCR扩增序列为616 bp,与预期结果相符... 为探讨湘西黄牛的分子遗传特征,寻找与湘西黄牛生长性状相关的分子标记,用PCR和基因测序技术检测了湘西黄牛IGF–IR基因部分序列的碱基突变,并采用PCR–RFLP方法进行了群体遗传多态性分析。结果表明:PCR扩增序列为616 bp,与预期结果相符,测序的序列中存在34个单核苷酸多态性(SNPs)位点,其中1个SNP已有报道(g.551G>T),33个SNPs为首次发现;g.551G>T位点在湘西黄牛群体中存在A和B 2个等位基因,A为优势等位基因,检测到AA、AB和BB 3种基因型;该位点在湘西黄牛群体中呈中度多态,达到了Hardy–Weinberg平衡状态(P>0.05);将湘西黄牛IGF–IR基因的多态性与其生长性状进行关联分析,结果表明,g.551G>T位点对湘西黄牛的体高、体长、胸围和体重无显著影响(P>0.05)。 展开更多
关键词 湘西黄牛 IGF–IR基因 群体遗传多态性 生长性状
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中国沿海真鲷群体遗传多样性的同工酶电泳分析 被引量:2
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作者 王志勇 王艺磊 +5 位作者 林利民 张子平 洪惠馨 张雅芝 于涛 张俭 《集美大学学报(自然科学版)》 CAS 北大核心 2003年第3期213-217,共5页
用同工酶电泳技术研究威海、厦门、北部湾3个海区真鲷群体的遗传多态性和群体之间的遗传趋异,共观测了LDH、MDH和EST 3种酶11个基因位点,其中LDH的3个位点在3个群体都为单态,MDH有1个位点、EST有2~4个位点表现多态,3个群体多态位点数... 用同工酶电泳技术研究威海、厦门、北部湾3个海区真鲷群体的遗传多态性和群体之间的遗传趋异,共观测了LDH、MDH和EST 3种酶11个基因位点,其中LDH的3个位点在3个群体都为单态,MDH有1个位点、EST有2~4个位点表现多态,3个群体多态位点数量和平均杂合度以威海群体最高(45.5%与0.161),厦门群体次之(27.3%与0.156),北部湾群体最低(27.3%与0.120)。北部湾与厦门海区的真鲷在遗传上较为相似,遗传距离0.012,威海海区真鲷与二者的差异较大,遗传距离分别达0.021(与厦门标本)和0.025(与北部湾标本)。 展开更多
关键词 中国沿海 真鲷 群体遗传多态性 同工酶电泳 基因位点 遗传趋异 遗传距离
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Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis 被引量:2
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作者 刘云国 李俊峰 叶乃好 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2011年第3期609-615,共7页
The genetic diversity of wild and hatchery populations of half-smooth tongue sole Cynoglossus semilaevis, based on observation of amplified fragment length polymorphism (AFLP) was described. Two hundred individuals fr... The genetic diversity of wild and hatchery populations of half-smooth tongue sole Cynoglossus semilaevis, based on observation of amplified fragment length polymorphism (AFLP) was described. Two hundred individuals from four wild populations, Laizhou (LZ), Weihai (WH), Qingdao (QD), Rizhao (RZ), and one hatchery population, Mingbo (MB), were screened using eight different AFLP primer combinations. A total of 384 loci were screened in the five studied populations. 48.4%, 51.3%, 50.7%, 49.3% and 45.8% of these loci were polymorphic among the individuals tested in the LZ, WH, QD, RZ and MB populations, respectively. The number of polymorphic loci detected by single primer combinations ranged from 17 to 35. The average heterozygosity of the LZ, WH, QD, RZ and MB populations was 0.072, 0.093, 0.092, 0.090 and 0.063, respectively. The WH population showed the highest genetic diversity in terms of total number of AFLP bands, total number of polymorphic bands, average heterozygosity and percentage of low frequency (0-0.2) polymorphic loci among all the populations, while the LZ population was the lowest among the wild populations. Compared with the wild populations, the hatchery population showed a low genetic viability. 展开更多
关键词 half-smooth tongue sole Cynoglossus semilaevis AFLP marker genetic characteristics
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Genetic Diversity Analysis of Cultured Populations of Jade Perch (Scortum bacoo) in China Using AFLP Markers 被引量:2
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作者 K. C. Chen L. S. Ma Y. Shi J. Zhao X. P. Zhu 《Journal of Agricultural Science and Technology》 2011年第4期455-461,共7页
Genetic diversity of three cultured populations of jade perch (Scortum bacoo) are studied using amplified fragment length polymorphism (AFLP) markers, which is Guangzhou(GZ) population, Foshan (FS) population ... Genetic diversity of three cultured populations of jade perch (Scortum bacoo) are studied using amplified fragment length polymorphism (AFLP) markers, which is Guangzhou(GZ) population, Foshan (FS) population and Qingdao(QD) population. Nine primer combinations were used and 385 fragments were detected. Among the 385 fragments, 80 bands (20.78%) were polymorphic. And it can be speculated that the genetic diversity of the three cultured populations of jade perch was very poor according to the gene genetic diversity among populations (Ht), the gene genetic diversity within populations (Hs) and Shannon-Weiner index (I). From gene differentiation (Gst), genetic distance (D), genetic similarity (5), and UPGMA analysis, it is found that the relationship among the three populations is very closed, and the difference in genetic diversity among the three populations is not significant. Further clustering relationships of the jade perch cultured populations also are correlated to historical-breeding observations and genetic relationships. The GZ population clustered together with the QD population first, then with FS population. 展开更多
关键词 AFLP Scortum bacoo cultured populations genetic diversity.
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SCANNING AND ANALYSIS OF MISMATCH DISTRIBUTION ON HUMAN GENOME
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作者 QINGYUN WANG YAYUAN XIAO +1 位作者 HAILU CHEN QIN ZOU 《International Journal of Biomathematics》 2012年第3期169-176,共8页
The mismatch distribution is a good descriptive summary statistic that describes the phenomena of population genetics. This article scanned mismatch distribution on human genome with single nucleotide polymorphism (... The mismatch distribution is a good descriptive summary statistic that describes the phenomena of population genetics. This article scanned mismatch distribution on human genome with single nucleotide polymorphism (SNP) data from the International HapMap Project. It is found that the abnormal mismatch distribution could imply some special segments on some chromosomes. One of the segments, on chromosome 8, was proved as an inversion. Other special segments may also imply some special structure on chromo- somes, such as duplication. The conjectures of other segments still need further research. 展开更多
关键词 Population genetics mismatch distribution single nucleotide polymorphism(SNP).
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