Objective To study effects of adenosine 5’-triphosphate (ATP) on cochlear function of guineapig. Methods After perfusion of ATP into perilymphatic spaces of the guinea pig cochlea, summating potential(SP) , cochlear ...Objective To study effects of adenosine 5’-triphosphate (ATP) on cochlear function of guineapig. Methods After perfusion of ATP into perilymphatic spaces of the guinea pig cochlea, summating potential(SP) , cochlear microphonic ( CM) , auditory nerve compound action potential ( CAP) , distortion product otoa-coustic emission (DPOAE) and auditory brainstem response (ABR) were measured. Results The resultsshowed concentration-dependent effect of ATP on the response alterations of bioelectric activity in cochlea. Adminis-tration of Immol/L ATP caused an increase both in the amplitude of the SP and in the threshold of ABR, a decreasein amplitude of the CAP and DPOAE. In addition, response alterations of the CAP and DPOAE showed in an inten-sity- and frequency-dependent manner, respectively. At levels of 20 - 70dB nHL sound intensity, lmmol/L ATPcaused a significant decrease in the CAP amplitude, while at moderate and high frequency ranges of 2 -8kHz it re-duced DPOAE amplitude significantly. 330μmol/L ATP also increased the threshold of ABR. Conclusion ATPthrough perilymphatic perfusion could inhibit cochlear function of guinea pig.展开更多
基金Supported by National Natural Science Foundation of China (30100206).
文摘Objective To study effects of adenosine 5’-triphosphate (ATP) on cochlear function of guineapig. Methods After perfusion of ATP into perilymphatic spaces of the guinea pig cochlea, summating potential(SP) , cochlear microphonic ( CM) , auditory nerve compound action potential ( CAP) , distortion product otoa-coustic emission (DPOAE) and auditory brainstem response (ABR) were measured. Results The resultsshowed concentration-dependent effect of ATP on the response alterations of bioelectric activity in cochlea. Adminis-tration of Immol/L ATP caused an increase both in the amplitude of the SP and in the threshold of ABR, a decreasein amplitude of the CAP and DPOAE. In addition, response alterations of the CAP and DPOAE showed in an inten-sity- and frequency-dependent manner, respectively. At levels of 20 - 70dB nHL sound intensity, lmmol/L ATPcaused a significant decrease in the CAP amplitude, while at moderate and high frequency ranges of 2 -8kHz it re-duced DPOAE amplitude significantly. 330μmol/L ATP also increased the threshold of ABR. Conclusion ATPthrough perilymphatic perfusion could inhibit cochlear function of guinea pig.
