目的：探讨溶酶体相关4次跨膜蛋白质B（lysosome-associated protein transmembrane 4 beta，LAPTM4B）基因多态性与肝癌易感性的关系．方法：应用病例对照研究方法，收集190例肝癌患者、190例慢性乙型肝炎患者、175例健康献血者全血，...目的：探讨溶酶体相关4次跨膜蛋白质B（lysosome-associated protein transmembrane 4 beta，LAPTM4B）基因多态性与肝癌易感性的关系．方法：应用病例对照研究方法，收集190例肝癌患者、190例慢性乙型肝炎患者、175例健康献血者全血，分离白细胞，提取基因组DNA，采用特异性引物PCR方法，扩增LAPTM4B第一外显子5′UTR内的部分序列，对三组人群进行分析研究．x^2检验分析肝癌组与对照组LAPTM4B的基因多态性和其他相关因素的相关性．结果：LAPTM4B等位基因在三组观察对象中的分布，^＊1和^＊2在健康对照组的频率分别是75．71％和24．29％，慢肝组73．16％和26、84％，肝癌组中66．84％和33.45％，三组比较等位基因分布频率有统计学意义，健康对照组与肝癌组比较有统计学意义（x^2=6．979，P=0．008）．LAPTM4B的基因型LAPTM4B1^＊1型、LAPTM4B^＊1／2混合型和LAPTM482^＊2型在肝癌组中的频率分别是37.9％、57.9％和4．2％、慢肝组50．5％、45．3％和4．2％、健康对照组56．6％、38.3％和5．1％，三组间三种基因型分布频率比较有统计学意义（x^2=14．854，P〈0．005）.结论：基因型^＊1／2和等位基因^＊2可能与肝癌的发生有关．展开更多
AIM:To evaluate the association between p53 codon 72 polymorphism and liver cancer risk by means of meta-analysis. METHODS:Two investigators independently searched the Medline,Embase and Chinese Biomedicine databases....AIM:To evaluate the association between p53 codon 72 polymorphism and liver cancer risk by means of meta-analysis. METHODS:Two investigators independently searched the Medline,Embase and Chinese Biomedicine databases.Summary odds ratios and 95%CI for p53 codon 72 polymorphism and liver cancer were calculated in fixedeffects model(Mantel-Haenszel method)and randomeffects model(DerSimonian and Laird method)when appropriate. RESULTS:This meta-analysis included 1115 liver cancer cases and 1778 controls.The combined results based on all studies showed that there was a statistically significant link between Pro/Pro genotype and liver cancer,but not between Arg/Arg or Pro/Arg genotype and liver cancer.When stratifying for race,similar results were obtained,i.e.patients with liver cancer had a significantly higher frequency of Pro/Pro genotype than non-cancer patients among Asians.After stratifying thevarious studies by control source,gender,family history of liver cancer and chronic hepatitis virus infection,we found that(1)patients among hospital-based studies had a significantly higher frequency of Pro/Pro and a significantly lower frequency of Arg/Arg genotype than individuals without cancer;(2)female patients with liver cancer had a significantly lower frequency of Arg/Arg and a higher frequency of Pro/Arg+Pro/Pro genotypes than female individuals without cancer;(3)subgroup analyses for family history of liver cancer did not reveal any significant association between p53 codon 72 polymorphism and liver cancer development;and(4) patients with negative hepatitis virus infection had a significantly higher frequency of Pro/Pro and a significantly lower frequency of Arg/Arg genotype than individuals without cancer. CONCLUSION:This meta-analysis suggests that the p53 codon 72 polymorphism may be associated with liver cancer among Asians.展开更多
AIM: To assess the association between Interleu-kin-10 (IL-10) gene IL-10-1082 (G/A), IL-10-592(C/A), IL-10-819 (T/C) polymorphisms and hepatocellular carcinoma (HCC) susceptibility.METHODS: Two investigators independ...AIM: To assess the association between Interleu-kin-10 (IL-10) gene IL-10-1082 (G/A), IL-10-592(C/A), IL-10-819 (T/C) polymorphisms and hepatocellular carcinoma (HCC) susceptibility.METHODS: Two investigators independently searched the Medline, Embase, China National Knowledge Infrastructure, and Chinese Biomedicine Database. Summary odds ratios (ORs) and 95% conf idence intervals (95% CIs) for IL-10 polymorphisms and HCC were cal-culated in a fixed-effects model (the Mantel-Haenszel method) and a random-effects model (the DerSimonian and Laird method) when appropriate. RESULTS: This meta analysis included seven eligiblestudies, which included 1012 HCC cases and 2308 controls. Overall, IL-10-1082 G/A polymorphism was not associated with the risk of HCC (AA vs AG + GG, OR = 1.11, 95% CI = 0.90-1.37). When stratifying for ethnicity, the results were similar (Asian, OR = 1.12, 95% CI = 0.87-1.44; non-Asian, OR = 1.10, 95% CI = 0.75-1.60). In the overall analysis, the IL-10 polymorphism at position -592 (C/A) was identified as a genetic risk factor for HCC among Asians; patients carrying the IL-10-592*C allele had an increased risk of HCC (OR = 1.29, 95% CI = 1.12-1.49). No association was observed between the IL-10-819 T/C polymorphism and HCC susceptibility (TT vs TC + CC, OR = 1.02, 95% CI = 0.79-1.32).CONCLUSION: This meta-analysis suggests that IL-10-592 A/C polymorphism may be associated with HCC among Asians. IL-10-1082 G/A and IL-10-819 T/C polymorphisms were not detected to be related to the risk for HCC.展开更多
文摘目的：探讨溶酶体相关4次跨膜蛋白质B（lysosome-associated protein transmembrane 4 beta，LAPTM4B）基因多态性与肝癌易感性的关系．方法：应用病例对照研究方法，收集190例肝癌患者、190例慢性乙型肝炎患者、175例健康献血者全血，分离白细胞，提取基因组DNA，采用特异性引物PCR方法，扩增LAPTM4B第一外显子5′UTR内的部分序列，对三组人群进行分析研究．x^2检验分析肝癌组与对照组LAPTM4B的基因多态性和其他相关因素的相关性．结果：LAPTM4B等位基因在三组观察对象中的分布，^＊1和^＊2在健康对照组的频率分别是75．71％和24．29％，慢肝组73．16％和26、84％，肝癌组中66．84％和33.45％，三组比较等位基因分布频率有统计学意义，健康对照组与肝癌组比较有统计学意义（x^2=6．979，P=0．008）．LAPTM4B的基因型LAPTM4B1^＊1型、LAPTM4B^＊1／2混合型和LAPTM482^＊2型在肝癌组中的频率分别是37.9％、57.9％和4．2％、慢肝组50．5％、45．3％和4．2％、健康对照组56．6％、38.3％和5．1％，三组间三种基因型分布频率比较有统计学意义（x^2=14．854，P〈0．005）.结论：基因型^＊1／2和等位基因^＊2可能与肝癌的发生有关．
文摘AIM:To evaluate the association between p53 codon 72 polymorphism and liver cancer risk by means of meta-analysis. METHODS:Two investigators independently searched the Medline,Embase and Chinese Biomedicine databases.Summary odds ratios and 95%CI for p53 codon 72 polymorphism and liver cancer were calculated in fixedeffects model(Mantel-Haenszel method)and randomeffects model(DerSimonian and Laird method)when appropriate. RESULTS:This meta-analysis included 1115 liver cancer cases and 1778 controls.The combined results based on all studies showed that there was a statistically significant link between Pro/Pro genotype and liver cancer,but not between Arg/Arg or Pro/Arg genotype and liver cancer.When stratifying for race,similar results were obtained,i.e.patients with liver cancer had a significantly higher frequency of Pro/Pro genotype than non-cancer patients among Asians.After stratifying thevarious studies by control source,gender,family history of liver cancer and chronic hepatitis virus infection,we found that(1)patients among hospital-based studies had a significantly higher frequency of Pro/Pro and a significantly lower frequency of Arg/Arg genotype than individuals without cancer;(2)female patients with liver cancer had a significantly lower frequency of Arg/Arg and a higher frequency of Pro/Arg+Pro/Pro genotypes than female individuals without cancer;(3)subgroup analyses for family history of liver cancer did not reveal any significant association between p53 codon 72 polymorphism and liver cancer development;and(4) patients with negative hepatitis virus infection had a significantly higher frequency of Pro/Pro and a significantly lower frequency of Arg/Arg genotype than individuals without cancer. CONCLUSION:This meta-analysis suggests that the p53 codon 72 polymorphism may be associated with liver cancer among Asians.
基金Supported by The National Natural Science foundation of China, No. 30901720
文摘AIM: To assess the association between Interleu-kin-10 (IL-10) gene IL-10-1082 (G/A), IL-10-592(C/A), IL-10-819 (T/C) polymorphisms and hepatocellular carcinoma (HCC) susceptibility.METHODS: Two investigators independently searched the Medline, Embase, China National Knowledge Infrastructure, and Chinese Biomedicine Database. Summary odds ratios (ORs) and 95% conf idence intervals (95% CIs) for IL-10 polymorphisms and HCC were cal-culated in a fixed-effects model (the Mantel-Haenszel method) and a random-effects model (the DerSimonian and Laird method) when appropriate. RESULTS: This meta analysis included seven eligiblestudies, which included 1012 HCC cases and 2308 controls. Overall, IL-10-1082 G/A polymorphism was not associated with the risk of HCC (AA vs AG + GG, OR = 1.11, 95% CI = 0.90-1.37). When stratifying for ethnicity, the results were similar (Asian, OR = 1.12, 95% CI = 0.87-1.44; non-Asian, OR = 1.10, 95% CI = 0.75-1.60). In the overall analysis, the IL-10 polymorphism at position -592 (C/A) was identified as a genetic risk factor for HCC among Asians; patients carrying the IL-10-592*C allele had an increased risk of HCC (OR = 1.29, 95% CI = 1.12-1.49). No association was observed between the IL-10-819 T/C polymorphism and HCC susceptibility (TT vs TC + CC, OR = 1.02, 95% CI = 0.79-1.32).CONCLUSION: This meta-analysis suggests that IL-10-592 A/C polymorphism may be associated with HCC among Asians. IL-10-1082 G/A and IL-10-819 T/C polymorphisms were not detected to be related to the risk for HCC.
