结肠移行性复合运动(colonic migrating motor complex,CMMC)是一种神经介导的、周期性的收缩和电活动,是推动小鼠结肠中粪便颗粒前进的主要动力.CMMC异常对胃肠动能紊乱,尤其是慢传输型便秘有重要影响.本文以CMMC产生和传播机制为切入...结肠移行性复合运动(colonic migrating motor complex,CMMC)是一种神经介导的、周期性的收缩和电活动,是推动小鼠结肠中粪便颗粒前进的主要动力.CMMC异常对胃肠动能紊乱,尤其是慢传输型便秘有重要影响.本文以CMMC产生和传播机制为切入点,集中探讨钻膜5轻色胺(5-hydroxytryptamine,5-HT)、肠肌间5-HT神经元、肠道Cajal间质细胞(interstitial cells of Cajal,ICC)网络、蠕动反射、隐匿反射对CMMC的影响,强调内在初级传入神经元(intrinsic primary afferent neurons,IPANs)、上行兴奋性神经通路和下行抑制性神经通路的重要作用,并介绍特定神经元活动检测新方法来佐证5-HT神经元在肠道动力方面的重要价值.展开更多
AIM： To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 （TRPM7） in the human gastrointestinal （GI） tract. METHODS： Conventional microel...AIM： To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 （TRPM7） in the human gastrointestinal （GI） tract. METHODS： Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajat （ICCs）. RESULTS： The human GI tract generated slow electrical waves and had ICCs which functioned as pacemak er cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB （2-aminoethoxydiphenyl borate） and La3＋, TRPM7 channel blockers, inhibited the slowwaves. Also, TRPM7 channels were expressed in ICCs in human tissue. CONCLUSION： These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the gen- eration of the slow waves.展开更多
文摘结肠移行性复合运动(colonic migrating motor complex,CMMC)是一种神经介导的、周期性的收缩和电活动,是推动小鼠结肠中粪便颗粒前进的主要动力.CMMC异常对胃肠动能紊乱,尤其是慢传输型便秘有重要影响.本文以CMMC产生和传播机制为切入点,集中探讨钻膜5轻色胺(5-hydroxytryptamine,5-HT)、肠肌间5-HT神经元、肠道Cajal间质细胞(interstitial cells of Cajal,ICC)网络、蠕动反射、隐匿反射对CMMC的影响,强调内在初级传入神经元(intrinsic primary afferent neurons,IPANs)、上行兴奋性神经通路和下行抑制性神经通路的重要作用,并介绍特定神经元活动检测新方法来佐证5-HT神经元在肠道动力方面的重要价值.
基金Supported by The Creative Research Initiative Center for Bio-Artificial Muscle of the Ministry of Education,Science and Technology (MEST) in Korea
文摘AIM： To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 （TRPM7） in the human gastrointestinal （GI） tract. METHODS： Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajat （ICCs）. RESULTS： The human GI tract generated slow electrical waves and had ICCs which functioned as pacemak er cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB （2-aminoethoxydiphenyl borate） and La3＋, TRPM7 channel blockers, inhibited the slowwaves. Also, TRPM7 channels were expressed in ICCs in human tissue. CONCLUSION： These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the gen- eration of the slow waves.
