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羌药胃草质量评价研究
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作者 谭承佳 田徽 +3 位作者 黄御莲 李玉莲 刘容西 马晓燕 《绵阳师范学院学报》 2023年第11期61-67,共7页
为了建立羌药胃草质量评价方法,采用显微法、TLC法定性鉴别牡荆素、异牡荆素,参照2020年版《中国药典》方法测定水分、总灰分、酸不溶性灰分、浸出物含量,采用HPLC法测定牡荆素含量、UV-Vis测定总黄酮含量.结果显示,显微特征明显,可见... 为了建立羌药胃草质量评价方法,采用显微法、TLC法定性鉴别牡荆素、异牡荆素,参照2020年版《中国药典》方法测定水分、总灰分、酸不溶性灰分、浸出物含量,采用HPLC法测定牡荆素含量、UV-Vis测定总黄酮含量.结果显示,显微特征明显,可见方晶、非腺毛、梯纹导管、木纤维、晶鞘纤维、淀粉粒等;TLC斑点清晰,分离度好;6批样品水分、总灰分、酸不溶性灰分、浸出物含量分别为1.50%~1.83%、4.63%~4.80%、1.16%~1.21%、15.64%~16.58%,牡荆素在0.101~0.505μg范围内线性关系良好(r=0.9999),平均加样回收率为100.69%,RSD为2.56%,平均含量为0.1149 mg/g;总黄酮(以芦丁计)在0.006~0.03 mg/mL范围内,线性关系良好(r=0.9996),平均加样回收率为97.2%,RSD为1.26%,平均含量为2.0641 mg/g.结论:该方法简便可靠,可用于羌药胃草的质量评价. 展开更多
关键词 胃草 牡荆素 异牡荆素 总黄酮 显微法
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三草调胃汤治疗胃癌前病变的疗效与机制研究
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作者 郑镔 蔡妍 +3 位作者 赵汗青 董祎 陈滨海 陈卫建 《浙江医学教育》 2024年第2期111-116,共6页
目的探讨三草调胃汤治疗胃癌前病变(precancerous lesions of gastric cancer,PLGC)的疗效与机制。方法对高通量基因表达数据库中GSE60427数据与前期实验小鼠胃组织芯片数据进行差异分析,筛选重叠基因并预测三草调胃汤治疗PLGC的靶点基... 目的探讨三草调胃汤治疗胃癌前病变(precancerous lesions of gastric cancer,PLGC)的疗效与机制。方法对高通量基因表达数据库中GSE60427数据与前期实验小鼠胃组织芯片数据进行差异分析,筛选重叠基因并预测三草调胃汤治疗PLGC的靶点基因,进行基因集富集分析。构建药物活性相关基因网络与聚类热图,预测三草调胃汤治疗PLGC的靶点为肿瘤坏死因子(tumor necrosis factor,TNF)与载脂蛋白E(apolipoprotein-E,APOE)。采用自身前后对照研究方法,选择2022年9月至2023年6月在浙江中医药大学附属第二医院陈卫建教授门诊治疗的123例PLGC患者为研究对象,均口服三草调胃汤。6个月后观察患者胃黏膜的病理学变化和血清APOE、TNF-α水平变化。结果PLGC胃组织的APOE基因表达水平较正常胃组织升高,三草调胃汤可以降低PLGC胃组织的APOE基因表达。PLGC与高APOE表达时炎症相关通路和脂质代谢通路均高度富集。TNF是三草调胃汤治疗PLGC的作用靶点,且在PLGC中呈现高表达,与APOE存在共表达关系。三草调胃汤治疗后,中、重度慢性萎缩性胃炎和中、重度肠化生患者数量均有所下降,不典型增生患者数量清零,TNF-α和APOE的表达水平均降低(均P<0.05)。结论三草调胃汤对PLGC疗效显著,其机制可能是通过降低TNF-α、APOE水平实现的。 展开更多
关键词 癌前病变 生物信息学 临床疗效 作用机制
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加味芍草平胃汤联合中药热包外敷治疗小儿肠系膜淋巴结炎湿热瘀阻证的临床疗效研究 被引量:12
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作者 王灿 钟振环 《河北中医药学报》 2023年第1期48-52,共5页
目的:研究加味芍草平胃汤联合中药热包外敷治疗小儿肠系膜淋巴结炎湿热瘀阻证的临床疗效。方法:选择2021年1月至2022年4月于石家庄市中医院儿科收治的70例肠系膜淋巴结炎证属湿热瘀阻的患儿为研究对象,按随机数字表法分成对照组和治疗组... 目的:研究加味芍草平胃汤联合中药热包外敷治疗小儿肠系膜淋巴结炎湿热瘀阻证的临床疗效。方法:选择2021年1月至2022年4月于石家庄市中医院儿科收治的70例肠系膜淋巴结炎证属湿热瘀阻的患儿为研究对象,按随机数字表法分成对照组和治疗组各35人。对照组使用枫蓼肠胃康颗粒治疗,治疗组则使用加味芍草平胃汤联合中药热包外敷治疗,2组均治疗2 w。研究2组小儿主症与次症积分的变化,对2组患儿的有效率、腹部彩超显示淋巴结纵径与横径的变化进行比较。结果:治疗2 w后,治疗组的总有效率为94.29%,明显高于对照组的80.00%(P<0.01);治疗组主症及次症积分相比于对照组明显降低(P<0.05),治疗组的淋巴结肿胀的缩小程度较对照组显著(P<0.05)。结论:加味芍草平胃汤联合中药热包外敷治疗小儿肠系膜淋巴结炎湿热瘀阻证的疗效显著。 展开更多
关键词 小儿肠系膜淋巴结炎 腹痛 湿热瘀阻型 加味芍 中药热包外敷 中医外治法
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HPLC法测定草香胃康胶囊中阿魏酸的含量 被引量:3
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作者 王瑞英 《中国当代医药》 2010年第25期47-48,共2页
目的:建立草香胃康胶囊中阿魏酸的HPLC测定方法。方法:采用AgilentTC-C18柱(150mm×4.6mm,5μm),流动相为甲醇-0.3%冰醋酸(35:65),流速为1.0ml/min,检测波长为320nm,柱温为25℃。结果:阿魏酸在0.023~0.460μg范围内线性关系良好(r=... 目的:建立草香胃康胶囊中阿魏酸的HPLC测定方法。方法:采用AgilentTC-C18柱(150mm×4.6mm,5μm),流动相为甲醇-0.3%冰醋酸(35:65),流速为1.0ml/min,检测波长为320nm,柱温为25℃。结果:阿魏酸在0.023~0.460μg范围内线性关系良好(r=0.9999),平均回收率为98.82%,RSD为1.53%。结论:该方法简便、快速、准确,可作为检测草香胃康胶囊中阿魏酸的有效方法。 展开更多
关键词 高效液相色谱法 康胶囊 阿魏酸
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RP-HPLC法测定草香胃康片中大黄素的含量 被引量:1
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作者 祝红 祝玲 《中国中医药现代远程教育》 2008年第5期423-424,共2页
目的建立草香胃康片中大黄素的RP-HPLC测定方法。方法色谱柱为ZORBAX XDB—C18(4.6×150mm,5um),以甲醇-水-磷酸(80:20:0.1)为流动相,柱温为室温,流速为1mL·min^-1。结果大黄素在0.11μg~1.1μg范围内与峰面积... 目的建立草香胃康片中大黄素的RP-HPLC测定方法。方法色谱柱为ZORBAX XDB—C18(4.6×150mm,5um),以甲醇-水-磷酸(80:20:0.1)为流动相,柱温为室温,流速为1mL·min^-1。结果大黄素在0.11μg~1.1μg范围内与峰面积有良好的线性关系,r=0.9998,平均回收率为99.93%,RSD为2.01%。结论该测定方法精密度高,重现性好,可用于测定大黄素的含量。 展开更多
关键词 康片 大黄素 RP-HPLC
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草香胃康胶囊质量标准研究 被引量:3
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作者 阮群 冯绮 甘业梅 《中成药》 CAS CSCD 北大核心 2007年第9期1328-1330,共3页
目的:制定草香胃康胶囊(决明子、阿魏、木香等)的质量标准。方法:用TLC鉴别决明子、木香、阿魏;以HPLC测定阿魏酸的含量,选用Agilent Eclipse XDB-C18柱(4.6 mm×250 mm,5μm),流动相为甲醇-1%冰醋酸溶液(30∶70),流速为1 m... 目的:制定草香胃康胶囊(决明子、阿魏、木香等)的质量标准。方法:用TLC鉴别决明子、木香、阿魏;以HPLC测定阿魏酸的含量,选用Agilent Eclipse XDB-C18柱(4.6 mm×250 mm,5μm),流动相为甲醇-1%冰醋酸溶液(30∶70),流速为1 mL/min,检测波长为320 nm。结果:TLC色谱中斑点清晰,易于识别;HPLC法精密度、重现性良好。阿魏酸在0.037 2-0.334 8μg范围内有较好的线性关系,平均回收率98.40%(RSD=1.41%)。结论:本法可有效控制草香胃康胶囊的质量。 展开更多
关键词 康胶囊 TLC HPLC 质量标准
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HPLC法测定草香胃康胶囊中大黄酚含量 被引量:2
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作者 谢东 何颂华 《广西医学》 CAS 2001年第3期459-461,共3页
目的 :为草香胃康胶囊建立专属性含量测定方法。方法 :采用 HPL C法测定。色谱柱 :Inertsil ODS- 3柱 ,流动相 :甲醇 - 0 .1%磷酸溶液 (82∶ 18) ,检测波长 :2 5 4nm。结果 :大黄酚在进样量为 0 .0 5~ 0 .5 0μg· 10μl- 1 范围... 目的 :为草香胃康胶囊建立专属性含量测定方法。方法 :采用 HPL C法测定。色谱柱 :Inertsil ODS- 3柱 ,流动相 :甲醇 - 0 .1%磷酸溶液 (82∶ 18) ,检测波长 :2 5 4nm。结果 :大黄酚在进样量为 0 .0 5~ 0 .5 0μg· 10μl- 1 范围内呈良好的线性关系 ;平均加样回收率为 98.85 % ,RSD为 0 .73% (n=6 )。结论 :本法操作简便 ,易行 ,具有实用性。 展开更多
关键词 康胶囊 大黄酚 含量测定 高级液相色谱
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HPLC法测定草香胃康胶囊中大黄酚的含量 被引量:3
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作者 黄宗海 王艳红 《广西中医学院学报》 2006年第4期63-64,共2页
[目的]建立草香胃康胶囊中大黄酚的含量测定方法。[方法]采用Agilent Eclipse XDB-C18柱(4.6mm×250mm,5μm),流动相为甲醇-0.1%磷酸(80:20);流速1.0ml/min,检测波长428nm。[结果]大黄酚在0.0754-0.6786μg范围内... [目的]建立草香胃康胶囊中大黄酚的含量测定方法。[方法]采用Agilent Eclipse XDB-C18柱(4.6mm×250mm,5μm),流动相为甲醇-0.1%磷酸(80:20);流速1.0ml/min,检测波长428nm。[结果]大黄酚在0.0754-0.6786μg范围内呈现良好的线性关系,r=0.9998,平均回收率为99.17%,RSD=0.4%。[结论]HPLC法简便、准确,重复性好,精密度高,可作为该制剂的质量控制。 展开更多
关键词 康胶囊 HPLC法 大黄酚
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草香胃康胶囊中7种成分的含量测定及聚类分析 被引量:5
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作者 张凯 马灵珍 《西北药学杂志》 CAS 2021年第2期184-188,共5页
目的建立能同时测定草香胃康胶囊中阿魏酸、木香烃内酯、去氢木香内酯、红镰霉素-6-O-β-龙胆二糖苷、决明子苷C、橙黄决明素和美决明子素含量的HPLC法。方法色谱柱:Kromasil C 18液相色谱柱(250 mm×4.6 mm,5μm);柱温:25℃;流动相... 目的建立能同时测定草香胃康胶囊中阿魏酸、木香烃内酯、去氢木香内酯、红镰霉素-6-O-β-龙胆二糖苷、决明子苷C、橙黄决明素和美决明子素含量的HPLC法。方法色谱柱:Kromasil C 18液相色谱柱(250 mm×4.6 mm,5μm);柱温:25℃;流动相:乙腈-1 mL·L^(-1)甲酸,梯度洗脱;流速:0.8 mL·min^(-1);检测波长:320 nm(检测阿魏酸),225 nm(检测木香烃内酯和去氢木香内酯),284 nm(检测红镰霉素-6-O-β-龙胆二糖苷、决明子苷C、橙黄决明素和美决明子素)。采用SPSS 26.0统计软件对草香胃康胶囊中7种成分的含量进行聚类分析。结果7种成分分别在0.56~14.00、1.89~47.25、2.03~50.75、5.91~147.75、2.27~56.75、1.69~42.25、1.31~32.75μg·mL^(-1)范围内线性关系良好(r≥0.9991);平均回收率分别为97.88%、98.56%、99.16%、100.04%、99.35%、96.98%、98.01%,RSD值分别为1.47%、1.22%、0.94%、0.69%、1.14%、1.03%、0.91%;10批样品聚类分析为2类。结论该方法操作简便、重复性好,可用于草香胃康胶囊中7种成分的含量测定。 展开更多
关键词 康胶囊 含量测定 阿魏酸 木香烃内酯 去氢木香内酯 红镰霉素-6-O-β-龙胆二糖苷 决明子苷C 橙黄决明素 美决明子素 聚类分析
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陈卫建治疗慢性萎缩性胃炎伴肠化生经验介绍 被引量:3
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作者 张高堆 陈滨海 +3 位作者 曹颖 程霜 邹丽珺 陈卫建(指导) 《新中医》 CAS 2021年第18期208-211,共4页
介绍陈卫建教授治疗慢性萎缩性胃炎伴肠化生的临床经验。陈教授认为,在病因病机上,慢性萎缩性胃炎伴肠化生,以脾胃虚弱为发病根本;久处湿地,饮食不节,易湿邪致病;肝气犯脾胃,气机升降失常;气血瘀滞贯穿整个过程。在治法上,当注重补虚培... 介绍陈卫建教授治疗慢性萎缩性胃炎伴肠化生的临床经验。陈教授认为,在病因病机上,慢性萎缩性胃炎伴肠化生,以脾胃虚弱为发病根本;久处湿地,饮食不节,易湿邪致病;肝气犯脾胃,气机升降失常;气血瘀滞贯穿整个过程。在治法上,当注重补虚培土,从补着手;注重气机通畅,以通为用;注重健脾祛湿,从湿而治;注重活血化瘀,从瘀而治。临证四诊合参,并通过胃镜和病理活检提示的胃黏膜萎缩性程度和肠化程度以指导疾病的治疗,创拟七白粉以改善瘀血,三草调胃汤以逆转肠化生。 展开更多
关键词 慢性萎缩性炎伴肠化生 病因病机 学术特色
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Induction of apoptosis and G2/M cell cycle arrest by oridonin in human gastric cancer BGC-823 cells 被引量:7
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作者 韩健 叶敏 +3 位作者 乔雪 吴婉莹 曲桂芹 果德安 《Journal of Chinese Pharmaceutical Sciences》 CAS 2007年第4期307-314,共8页
Aim To investigate in vitro apoptosis-induction effects of oridonin on gastric tumor cells BGC-823 and its effects on cell cycle, mitochondrial membrane potential and intracellular Ca^2+ to shed light on the mode of ... Aim To investigate in vitro apoptosis-induction effects of oridonin on gastric tumor cells BGC-823 and its effects on cell cycle, mitochondrial membrane potential and intracellular Ca^2+ to shed light on the mode of its anticancer action. Methods The MTT method was used to investigate the inhibitory effect of oridonin on BGC-823 cells. The apoptosis-induction effect was evaluated by confocal laser microscopy and flow cytometry. The change of mitochondrial membrane potential and the increase of intracellular Ca^2+ were assessed by fluorescence probe rhodamine123 and Fluo 3-AM, respectively, with flow cytometry. The expression of apoptosis and cell cycle related proteins was studied using western blotting. Results Oridonin inhibited BGC-823 cells growth with IC50 of 22.21 p, mol.L^-1. It induced apoptosis in a dose-dependent manner. In addition, it decreased mitochondria membrane potential, increased intracellular Ca^2+, and activated pro-caspase 3. BGC-823 cells were arrested in G2/M cell cycle phase with lower expression of cyclin A protein. The up-regulation of p53 was observed before apoptosis and cell cycle arrest occurred. Conclusion Oridonin inhibits the proliferation of BGC-823 cells through G2/M cell cycle arrest and apoptosis induction, which is mediated by influx of Ca^2+, up-regulation of p53, activation of caspase-3, and down-regulation of cyclin A. 展开更多
关键词 ORIDONIN Human gastric cancer APOPTOSIS Cell cycle arrest P53 Cyclin A
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Molecular forms of trefoil factor 1 in normal gastric mucosa and its expression in normal and abnormal gastric tissues 被引量:6
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作者 Jian-Lin Ren Jin-Yan Luo +2 位作者 Ya-Pi Lu Lin Wang Hua-Xiu Shi 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第45期7361-7364,共4页
AIM: To study the molecular forms of trefoil factor 1 (TFF1) in normal gastric mucosa and its expression in normal and abnormal gastric tissues (gastric carcinoma, atypical hyperplasia and intestinalized gastric m... AIM: To study the molecular forms of trefoil factor 1 (TFF1) in normal gastric mucosa and its expression in normal and abnormal gastric tissues (gastric carcinoma, atypical hyperplasia and intestinalized gastric mucosa) and the role of TFF1 in the carcinogenesis and progression of gastric carcinoma and its molecular biological mechanism underlying gastric mucosa protection. METHODS: The molecular forms of TFF1 in normal gastric mucosa were observed by Western blot. The expression of TFF1 in normal and abnormal gastric tissues (gastric carcinoma, atypical hyperplasia and intestinalized gastric mucosa) was also assayed by immunohistochemical method. The average positive AO was estimated by Motic Images Advanced 3.0 software. RESULTS: Three patterns of TFF1 were found in normal gastric mucosa: monomer, dimmer, and TFF1 compound whose molecular weight is about 21 kDa. The concentration of TFF1 compound was the highest among these three patterns. TFF1 was expressed mainly in epithelial cytoplasm of the mucosa in gastric body and antrum, especially around the nuclei. The closer the TFF1 to the lumen, the higher the expression of TFF1, The expression of TFF1 in peripheral tissue of gastric carcinoma (0.51 ± 0.07) was higher than that in normal gastric mucosa (0.44 ± 0.06, P 〈 0.001). The expression of TFF1 in gastric adenocarcinoma was positively related to the differentiation of adenocarcinoma. The lower the differentiation of adenocarcinoma was, the weaker the expression of TFF1. No TFF1 was expressed in poorlydifferentiated adenocarcinoma. The expression of TFF1 in moderately-well differentiated adenocarcinoma (0.45 ± 0.07) was a little lower than that in normal mucosa (P 〉 0.05). The expression of TFF1 in gastric mucosa with atypical hyperplasia (0.57 ± 0.03) was significantly higher than that in normal gastric mucosa (P 〈 0.001). No TFF1 was expressed in intestinalized gastric mucosa. There was no statistically significant difference between the expressions of TFFI in gastric mucosa around the intestinalized tissue (0.45 ± 0.07) and normal gastric mucosa (P 〉 0.05). CONCLUSION: TFF1 is expressed mainly in epithelial cytoplasm of the mucosa in gastric body and antrum. Its main pattern is TFF1 compound, which may have a greater biological activity than monomer and dimer. The expression of TFF1 in peripheral mucosa of gastric ulcer is higher than that in mucosa 5 cm beyond the ulcer, indicating that TFF1 plays an important part in protection and restitution of gastric mucosa. The expression of TFF1 is increased in peripheral tissues of gastric carcinoma and gastric mucosa with atypical hyperplasia, but is decreased in cancer tissues, implying that TFF1 may be related to suppression and differentiation of carcinoma. The weaker expression of TFF1 in poorly-differentiated carcinoma may be related to the destruction of glands and cells in cancer tissues and the decrease in secretion of TFF1. 展开更多
关键词 Trefoil factor 1 Gastric mucosa protection Carcinoma suppression
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Interaction models of CYP1A1, GSTMl polymorphisms and tobacco smoking in intestinal gastric cancer 被引量:2
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作者 Jing Shen Run-Tian Wang +2 位作者 Yao-Chu Xu Li-Wei Wang Xin-Ru Wang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第38期6056-6060,共5页
AIM: To explore the interaction models of the cytochrome P-450 (CYP) 1A1 Valvariant and glutathione S-transferase (GST) M1 null polymorphisms with tobacco smoking in the occurrence of intestinal gastric cancer. M... AIM: To explore the interaction models of the cytochrome P-450 (CYP) 1A1 Valvariant and glutathione S-transferase (GST) M1 null polymorphisms with tobacco smoking in the occurrence of intestinal gastric cancer. METHODS: A community-based case-control study was conducted in Yangzhong. Subjects included 114 intestinal types of gastric cancer with endoscopic and pathological diagnosis during January 1997 and December 1998, and 693 controls selected from their spouse, siblings or siblingsin-law who had no history of digestive system cancer. Logistic regression was used to estimate the interaction models. RESULTS: The frequency of the CYPIA1 Valvariant allele in cases did not differ from that in controls. The OR of GSTM1 null genotype was 2.0 (95% confidence interval [95%CI]: 1.2-3.1, P〈0.01). It showed a significant type 2 form of interaction model when both CYPIA1 Valvariant allele and former tobacco smoking existed (i.e., among the multiplicative effects, the disease risk is increased by the tobacco exposure alone but not by the CYPIA1 variant alone). The interaction index y was 2.8, and OReg (95%CI) was 5.0 (1.9-13.4). GSTM1 null genctype and former tobacco smoking were significant in a type 4 interaction model (i.e., the disease risk is increased by GSTM1 null genotype or tobacco exposure alone among the multiplicative effects). The interaction index y and OReg (95%CI) were 3.4 and 8.4 (3.4-20.9), respectively.CONCLUSION: Different interaction models of CYPIA1 Valvariant allele and GSTM1 null genotype with tobacco smoking will contribute to understanding carcinogenic mechanism, but there is a need to further investigate in larger scale studies. 展开更多
关键词 Interaction models CYP1A1 GSTM1 Tobacco smoking Intestinal gastric cancer
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Gastric mucosal injury due to hemorrhagic reperfusion and efficacy of Salvia miltiorrhizae extract F and cimetidine 被引量:8
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作者 Li-HongZhang Chang-BaiYao +1 位作者 Ming-QiGao He-QuanLi 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第18期2830-2833,共4页
AIM: To observe the gastric mucosal injury caused by hemorrhagic shock and reperfusion and to compare the effect between Salvia miltiorrhizae extract F (SEF) and cimetidine (CI) on it. METHODS: A model of hemorrhage/r... AIM: To observe the gastric mucosal injury caused by hemorrhagic shock and reperfusion and to compare the effect between Salvia miltiorrhizae extract F (SEF) and cimetidine (CI) on it. METHODS: A model of hemorrhage/reperfusion injury was produced by Itoh method. Wistar rats were randomly divided into three groups: 0.9% sodium chloride treatment group (NS group), SEF treatment group (SEF group), and CI treatment group (CI group). Saline, SEF and CI were injected respectively. The index of gastric mucosal lesions (IGML) was expressed as the percentage of lesion area in the gastric mucosa. The degree of gastric mucosal lesions was categorized into grades 0, 1, 2, 3. Atom absorption method was used to measure the intracellular calcium content. Radioimmunoassay was used to measure the concentrations of prostaglandins. RESULTS: IGML (%) and grade 3 (%) were 23.18±6.82, 58.44±9.07 in NS group, 4.42±1.39, 20.32±6.95 in SEF group and 3.74±1.56, 23.12±5.09 in CI group, and the above parameters in SEF group and CI group decreased significantly (IGML: SEF vs NS, t=6.712, P=0.000<0.01; CI vs NS, t=6.943, P=0.000<0.01; grade 3: SEF vs HS, t=8.386, P=0.000; CI vs HS, t=8.411, P= 0.000), but the grade 0 and grade 1 damage in SEF group (22.05±5.96, 34.12±8.12) and CI group (18.54±4.82, 30.15±7.12) were markedly higher than those in NS group (3.01±1.01, 8.35±1.95; grade 0: SEF vs HS, t=8.434, P=0.000<0.01; CI vs NS, t=7.950, P=0.000<0.01; grade 1: SEF vs NS, t =8.422, P=0.000<0.01; CI vs NS, t=8.448, P=0.000<0.01). The intracellular calcium content (μg/mg) in SEF group (0.104±0.015) and CI group (0.102±0.010) was markedly lower than that in NS group (0.131±0.019, SEF vs NS, t=2.463, P=0.038<0.05; CI vs HS, t=3.056, P=0.017<0.05). The levels (pg/mg) of PGE_2, 6-keto-PGF_(1α) and 6-keto-PGF_(1α)/TXB_2 were 540±183, 714±124,17.38±5.93 in NS group and 581±168, 737±102, 19.04±8.03 in CI group, 760±192,1 248±158, 33.42±9.24 in SEF group, and the above parameters in SEF group markedly raised (PGE_2: SEF vs NS, t=2.282, P=0.046<0.05; SEF vs CI, t=2.265, P=0.047<0.05; 6-keto-PGF_(1α): SEF vs NS, t=6.583, P=0.000<0.000; SEF vs CI, t=6.708, P=0.000<0.01; 6-keto-PGF_(1α)/TXB_2: SEF vs NS, t=3.963, P=0.003<0.001; SEF vs Cl, t=3.243, P=0.009<0.01), whereas TXB_2 level in SEF group (45.37±7.54) was obviously lower than that in NS group (58.28±6.74, t=3.086, P=0.014<0.05) and CI group (54.32±6.89, t=2.265, P=0.047<0.05). No significant difference was shown between NS group and CI group (PGE_2: t=0.414, P=0.688>0.05; 6-keto-PGF_(1α): t=0.310, P=0.763>0.05; TXB_2: t=1.099, P=0.298>0.05; 6-keto-PGF_(1α)/TXB_2: t=0.372, P=0.718>0.05). CONCLUSION: Both SEF and CI could inhibit reperfusioninduced injury in gastric mucosa, but with different mechanisms. SEF could not only enhance the protective effect of gastric mucosa, but also abate the injury factors, while CI can only abate the injury factors. 展开更多
关键词 Hemorrhagic shock Reperfusion injury Gastric mucosa Radix Salvia miltiorrhizae CIMETIDINE
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Advances in TCM Research and Treatment of Gastropathies Associated with Helicobacter Pylorum
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作者 阳媚 毛树章 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2000年第2期152-157,共6页
The etiological role of helicobacter pylorum (HP)in gastropathies has aroused great interest in themedical circle since its first isolation in biopsyspecimen from human gastric mucosa by Wamen andMarshall in 1983.The ... The etiological role of helicobacter pylorum (HP)in gastropathies has aroused great interest in themedical circle since its first isolation in biopsyspecimen from human gastric mucosa by Wamen andMarshall in 1983.The previous studies havedemonstrated that HP is the main etiologic factor ofchronic gastritis,and it is closely related with theoccurrence of peptic ulcers.In the present paper,therecent achievements in TCM researches andtreatments of gastropathies associated with HP-induced infections are summarized as follows. 展开更多
关键词 Helicobacter pylori Drugs Chinese Herbal Gastritis Atrophic Helicobacter Infections Humans Stomach Ulcer
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Gastroprotective activity of Nigella sativa L oil and its constituent, thymoquinone against acute alcohol-induced gastric mucosal injury in rats 被引量:9
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作者 Mehmet Kanter Halit Demir +1 位作者 Cengiz Karakaya Hanefi Ozbek 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第42期6662-6666,共5页
AIM: To evaluate the role of reactive oxygen species in the pathogenesis of acute ethanol-induced gastric mucosal lesions and the effect of Nigella sativa L oil (NS) and its constituent thymoquinone (TQ) in an experim... AIM: To evaluate the role of reactive oxygen species in the pathogenesis of acute ethanol-induced gastric mucosal lesions and the effect of Nigella sativa L oil (NS) and its constituent thymoquinone (TQ) in an experimental model.METHODS: Male Wistar albino rats were assigned into 4groups. Control group was given physiologic saline orally (10 mL/kg body weight) as the vehicle (gavage); ethanol group was administrated 1 mL (per rat) absolute alcohol by gavage; the third and fourth groups were given NS (10 mL/kg body weight) and TQ (10 mg/kg body weight p.o) respectively 1 h prior to alcohol intake. One hour after ethanol administration, stomach tissues were excised for macroscopic examination and biochemical analysis.RESULTS: NS and TQ could protect gastric mucosa against the injurious effect of absolute alcohol and promote ulcer healing as evidenced from the ulcer index (UI) values. NS prevented alcohol-induced increase in thiobarbituric acid-reactive substances (TBARS), an index of lipid peroxidation. NS also increased gastric glutathione content (GSH), enzymatic activities of gastric superoxide dismutase (SOD) and glutathione-S-transferase (GST). Likewise, TQ protected against the ulcerating effect of alcohol and mitigated most of the biochemical adverse effects induced by alcohol in gastric mucosa, but to a lesser extent than NS. Neither NS nor TQ affected catalase activity in gastric tissue.CONCLUSION: Both NS and TQ, particularly NS can partly protect gastric mucosa from acute alcohol-induced mucosal injury, and these gastroprotective effects might be induced, at least partly by their radical scavenging activity. 展开更多
关键词 Nigella sativa THYMOQUINONE ULCER ANTIOXIDANT Rat
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Liver function alterations after laparoscopy-assisted gastrectomy for gastric cancer and its clinical significance 被引量:7
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作者 Gui-Ae Jeong Gyu-Seok Cho +3 位作者 Eung-Jin Shin Moon-Soo Lee Hyung-Chul Kim Ok-Pyung Song 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第3期372-378,共7页
AIM: To evaluate the factors associated with liver function alterations after laparoscopy-assisted gastrectomy (LAG) for gastric cancer. METHODS: We collected the data of gastrectomy patients with gastric cancer and d... AIM: To evaluate the factors associated with liver function alterations after laparoscopy-assisted gastrectomy (LAG) for gastric cancer. METHODS: We collected the data of gastrectomy patients with gastric cancer and divided them into 2 groups: open gastrectomy (OG) and LAG. We also collected the data of patients with colon cancer to evaluate the effect of liver manipulations during surgery on liver function alterations. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin, and alkaline phosphatase were measured on the preoperative day and postoperative day 1 (POD1), POD3, POD5, and POD7. RESULTS: No changes in liver function were observed after the operation in patients with colon cancer (n = 121). However, in gastric cancer patients (n = 215), AST and ALT levels increased until POD5 compared to those in colon cancer patients and these findings were observed both in the LAG and OG without a significant difference except at POD1. The mean hepatic enzyme levels at POD1 in the LAG group were significantly higher than those in the OG group (P = 0.047 for AST and P = 0.039 for ALT). The factors associated with elevated ALT on POD1 in patients with gastric cancer were body mass index (P < 0.001), operation time (P < 0.001), intraoperative hepatic injury (P = 0.048), and ligation of an aberrant left hepatic artery (P = 0.052) but not type of operation (OG vs LAG, P = 0.094). CONCLUSION: We conclude that the liver function alteration after LAG may have been caused by direct liver manipulation or aberrant hepatic artery ligation rather than the CO2 pneumoperitoneum. 展开更多
关键词 Gastric cancer Liver function PNEUMOPERITONEUM Laparoscopy-assisted gastrectomy
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AN ANALYSIS FOR DEATH CAUSES IN 45 CASES OF LIVER CANCER TREATED WITH TRADITIONAL CHINESE DRUGS
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作者 杨宗艳 隋希文 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 1999年第2期97-99,共3页
Among the 165 cases of late-stage liver cancer treated in our hospital,65(39.4%)died,with an average survival time of 8.1 months and a median survival time of 7 months.Among the 65 dead patients,45 were treated with t... Among the 165 cases of late-stage liver cancer treated in our hospital,65(39.4%)died,with an average survival time of 8.1 months and a median survival time of 7 months.Among the 65 dead patients,45 were treated with traditional Chinese drugs and 20 withwestern medicine.The average survival time was 8.4 months in the former and 7.3months in the latter group.The direct causes of death for the 65 patients were hepaticcoma,severe hemorrhage of the upper digestive tract,Heyd’s syndrome,hepatorrhexis,respiratory failure,cardiac failure,etc.The incidence rates of hemorrhage of the upperdigestive tract and hepatorrhexis in the 45 patients treated with traditional Chinese drugswere obviously lower than those treated with western medicine. 展开更多
关键词 Cause of Death Adult Aged China Drugs Chinese Herbal Gastrointestinal Hemorrhage Hepatic Encephalopathy Humans Liver Diseases Liver Neoplasms Middle Aged Rupture Spontaneous
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Empirical Prescriptions Used in Treatment of Epigastralgia due to Blood Stasis of Deficiency Type
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作者 李霞 陈丽萍 +1 位作者 杨文思 王新中 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2000年第2期110-114,共5页
Epigastralgia,a commonly-seen symptom inchronic gastritis or gastric ulcer,and usually causesinjury of the vital-qi and thereby deficiency of thehuman body due to its chronicity.The deficiency isdivided into deficienc... Epigastralgia,a commonly-seen symptom inchronic gastritis or gastric ulcer,and usually causesinjury of the vital-qi and thereby deficiency of thehuman body due to its chronicity.The deficiency isdivided into deficiency of yin,yang,qi,and blood.Asis said in A Guide to Clinical Practice with MedicalRecords(临证指南医案),illnesses at the early stageaffect channels which dominate qi while at the latestage collaterals which dominate blood,and 展开更多
关键词 Drugs Chinese Herbal Female GASTRITIS Gastritis Atrophic Humans Male Middle Aged QI Yang Deficiency Yin Deficiency
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Inclusion Levels of Fermented Apple Bagasse on in Vitro Rumen Fermentation of Alfalfa Hay
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作者 Yamicela Castillo-Castillo Oscar Ruiz-Barrera +4 位作者 Eduviges Burrola-Barraza Claudio Arzola-Alvarez Agustin Corral-Luna Carlos Rodriguez-Muela Manuel Murillo-Ortiz 《Journal of Agricultural Science and Technology(A)》 2015年第1期40-46,共7页
The aim of this study was to assess the effect of inclusion of fermented apple bagasse (FAB) obtained through solid state fermentation on pH, ammonia nitrogen (N-NH3), volatile fatty acids (VFA) content, in vitr... The aim of this study was to assess the effect of inclusion of fermented apple bagasse (FAB) obtained through solid state fermentation on pH, ammonia nitrogen (N-NH3), volatile fatty acids (VFA) content, in vitro dry matter digestibility (IVDMD), lactic acid and microbial counting of alfalfa hay under in vitro rumen environment; four levels of FAB were evaluated (0, 0.25, 0.50 and 0. 75 g/dry matter of FAB) replacing 1.5 g dry matter (DM) of alfalfa hay and incubated at different fermentation times (0, 4, 8, 12 and 24 h) using a complete random design with repeated measures on time. Counts of live yeast colonies (6.08, 6.33, 6.24 and 6.51 CFU/mL expressed as log 10) was higher when FAB was included in the different levels up to the 12 h of fermentation (P 〈 0.0001); lactic acid content also increased as FAB was included in the different levels (10.61, 13.86, 16.84 and 14.57μg/mL) up to the 12 h of incubation (P 〈 0.001). Nevertheless, the other variables measured as pH, N-NH3, VFA, IVDMD, total bacteria and fungi counts, were not affected by the treatments. It is concluded that substitution of FAB by alfalfa hay in an in vitro rumen ecosystem positively modified live yeast colonies and lactic acid concentration, without effect on the other fermentative and microbial parameters of the in vitro rumen environment, but considering mixes of FAB and alfalfa hay as a quality ingredient for the feeding of ruminants. 展开更多
关键词 Apple bagasse ALFALFA MICROBIAL solid state fermentation.
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