Using the mouse whole embryo culturetechnique,the teratogenesis induced bycyclophosphamide（5,10,15,20mg/kg,ip）andits mechanism were investigated in mice on day8of gestation.
目的探讨不同体外培养方法对小鼠去透明带胚胎发育的影响。方法以胚胎各阶段发育率、囊胚率和囊胚细胞数作为衡量指标,对比微滴单卵法、微滴群卵法、微滴单卵+群卵法、m WOW培养法(the modified"well of well"system)四种体...目的探讨不同体外培养方法对小鼠去透明带胚胎发育的影响。方法以胚胎各阶段发育率、囊胚率和囊胚细胞数作为衡量指标,对比微滴单卵法、微滴群卵法、微滴单卵+群卵法、m WOW培养法(the modified"well of well"system)四种体外培养方法 ,探讨不同体外培养方法对去透明带胚胎发育的影响。结果 m WOW培养法、微滴单卵+群卵法、微滴单卵法这三种方法之间的发育率、囊胚率以及囊胚数均有显著差异(P<0.05)。结论m WOW法与其他三种方法相比,更适合作为体外培养去透明带小鼠8-细胞以后阶段的方法 ,以及用于体内移植小鼠胚胎的体外培养方法。展开更多
自从六十年代发现孕妇服用反应停(thalidomiode)后出生数千名短肢畸形的后代以来,化学物质的致畸胎作用引起了人们的严重关住,随着工业的发展,大量新化学物质和环境污染物不断进入人类生产及生活环境,原有传统的整体动物致畸试验方法由...自从六十年代发现孕妇服用反应停(thalidomiode)后出生数千名短肢畸形的后代以来,化学物质的致畸胎作用引起了人们的严重关住,随着工业的发展,大量新化学物质和环境污染物不断进入人类生产及生活环境,原有传统的整体动物致畸试验方法由于实验周期长已适应不了评价日新月异的新化学物质的需要,因此,筛选快速而又敏感特异的新方法已成为现代毒理学的重要课题之一。七十年代初著名的胚胎培养之父New发展了哺乳动物全胚胎旋转培养技术(wholeembryo rotator culture of mammaliam)并应用于评价化学物质的致畸性,获得了成功。展开更多
Nutrient media and culture conditions have been defined for ovules 3 and 5 d after pollination and embryos of Gossypium herbaceum respectively. The technique was then used to produce interspecific hybrids between a cu...Nutrient media and culture conditions have been defined for ovules 3 and 5 d after pollination and embryos of Gossypium herbaceum respectively. The technique was then used to produce interspecific hybrids between a cultivated Gossypium herbaceum and a wild species, G. stocksii. The hybrid plants were transferred to field and they exhibited most of the characters of the pollen parent i.e.G. stocksii.展开更多
文摘Using the mouse whole embryo culturetechnique,the teratogenesis induced bycyclophosphamide（5,10,15,20mg/kg,ip）andits mechanism were investigated in mice on day8of gestation.
文摘目的探讨不同体外培养方法对小鼠去透明带胚胎发育的影响。方法以胚胎各阶段发育率、囊胚率和囊胚细胞数作为衡量指标,对比微滴单卵法、微滴群卵法、微滴单卵+群卵法、m WOW培养法(the modified"well of well"system)四种体外培养方法 ,探讨不同体外培养方法对去透明带胚胎发育的影响。结果 m WOW培养法、微滴单卵+群卵法、微滴单卵法这三种方法之间的发育率、囊胚率以及囊胚数均有显著差异(P<0.05)。结论m WOW法与其他三种方法相比,更适合作为体外培养去透明带小鼠8-细胞以后阶段的方法 ,以及用于体内移植小鼠胚胎的体外培养方法。
文摘自从六十年代发现孕妇服用反应停(thalidomiode)后出生数千名短肢畸形的后代以来,化学物质的致畸胎作用引起了人们的严重关住,随着工业的发展,大量新化学物质和环境污染物不断进入人类生产及生活环境,原有传统的整体动物致畸试验方法由于实验周期长已适应不了评价日新月异的新化学物质的需要,因此,筛选快速而又敏感特异的新方法已成为现代毒理学的重要课题之一。七十年代初著名的胚胎培养之父New发展了哺乳动物全胚胎旋转培养技术(wholeembryo rotator culture of mammaliam)并应用于评价化学物质的致畸性,获得了成功。
文摘Nutrient media and culture conditions have been defined for ovules 3 and 5 d after pollination and embryos of Gossypium herbaceum respectively. The technique was then used to produce interspecific hybrids between a cultivated Gossypium herbaceum and a wild species, G. stocksii. The hybrid plants were transferred to field and they exhibited most of the characters of the pollen parent i.e.G. stocksii.
