A four-point bending apparatus is used to investigate the effects of stretching on collagen synthesis, mineralization and differentiation of osteoblasts. Cells are stretched at 1500 με for 24 hours. The responses of...A four-point bending apparatus is used to investigate the effects of stretching on collagen synthesis, mineralization and differentiation of osteoblasts. Cells are stretched at 1500 με for 24 hours. The responses of osteoblasts to mechanical signal of physiological stretching are evaluated from three aspects: collagen production, extracellular inorganic calcium secretion and ALP activity. The results show that osteoblasts decrease the collagen synthesis, calcium secretion and ALP activity compared to the control cells (65.82%,73.51%,48.10% respectively), confirming that cyclic stretching at 1500 με inhabits the physiological activity of osteoblasts.展开更多
Endostatin is a natural occurred angiogenesis inhibitor derived from collagenXVIII. So far its function during the angiogenesis process of bone formation and arthropathy has not been well studied yet. The present stud...Endostatin is a natural occurred angiogenesis inhibitor derived from collagenXVIII. So far its function during the angiogenesis process of bone formation and arthropathy has not been well studied yet. The present study addresses the function of endostatin in rabbit articular chondrocytes (RAC). We found that endostatin can promote RAC adhesion and spreading as well as its proliferation. In monolayer cultured RAC, CollagenII, TIMP1 and collagenXVIII transcription were up regulated by endostatin while collagenI and MMP9 were down regulated. Moreover collagenXVIII and endostatin antigens are present at synovial fluid. These findings indicate new function of endostatin as a homeostatic factor in cartilage metabolism.展开更多
文摘A four-point bending apparatus is used to investigate the effects of stretching on collagen synthesis, mineralization and differentiation of osteoblasts. Cells are stretched at 1500 με for 24 hours. The responses of osteoblasts to mechanical signal of physiological stretching are evaluated from three aspects: collagen production, extracellular inorganic calcium secretion and ALP activity. The results show that osteoblasts decrease the collagen synthesis, calcium secretion and ALP activity compared to the control cells (65.82%,73.51%,48.10% respectively), confirming that cyclic stretching at 1500 με inhabits the physiological activity of osteoblasts.
文摘Endostatin is a natural occurred angiogenesis inhibitor derived from collagenXVIII. So far its function during the angiogenesis process of bone formation and arthropathy has not been well studied yet. The present study addresses the function of endostatin in rabbit articular chondrocytes (RAC). We found that endostatin can promote RAC adhesion and spreading as well as its proliferation. In monolayer cultured RAC, CollagenII, TIMP1 and collagenXVIII transcription were up regulated by endostatin while collagenI and MMP9 were down regulated. Moreover collagenXVIII and endostatin antigens are present at synovial fluid. These findings indicate new function of endostatin as a homeostatic factor in cartilage metabolism.
