A gene （NANOC-D6D） encoding a desaturase that removes two hydrogen atoms from fatty acids at delta 6 position was isolated from a cDNA library of Nannochloropsis oculata （Droop） D. J. Hibberd （Eustigmatophyceae）...A gene （NANOC-D6D） encoding a desaturase that removes two hydrogen atoms from fatty acids at delta 6 position was isolated from a cDNA library of Nannochloropsis oculata （Droop） D. J. Hibberd （Eustigmatophyceae）. The unicellular marine microalga N. oculata synthesizes rich long chain polyunsaturated fatty acids （LCPUFAs）, including eicosapentaenoic acid （20：5n-3, EPA）. The deduced protein contains 474 amino acids that fold into 4 trans-membrane domains. The neighbor-joining phylogenetic tree indicates that NANOC-D6D is phylogenetically close to the delta-6 fatty acid desaturase of marine microalgae such as Glossomastix chrysoplasta, Thalassiosira pseudonana, and Phaeodactylum tricornutum. The gene was expressed in Saccharomyces cerevisiae INVScl to verify the substrate specificity of NAN OC-D6D. Our results suggest that the recombinant NANOC-D6D simultaneously desaturates linoleic acid （LA） and a-linolenic acid （ALA）.展开更多
An Australian food composition database, AUSNUT1999, does not include long chain omega-3 polyunsaturated fatty acid （LC omega-3 PUFA） data. Measurement of the fatty acid content of diets initially analysed using AUS...An Australian food composition database, AUSNUT1999, does not include long chain omega-3 polyunsaturated fatty acid （LC omega-3 PUFA） data. Measurement of the fatty acid content of diets initially analysed using AUSNUT1999 requires conversion to AUSNUT2007, an updated database inclusive of LC omega-3 PUFA. The aim of this study was to convert clinical trial dietary data from AUSNUT1999 to AUSNUT2007 and measure LC omega-3 PUFA intake. Clinical trial diet history （DH） data was converted from AUSNUTI999 to 2007 using a staged approach. Macronutrient intake from AUSNUTI999 and 2007 were calculated and compared via paired t-tests and Wilcoxon Signed Ranks tests. Mean dietary LC omega-3 PUFA intake and the percentage contribution of food groups to total LC omega-3 PUFA were then calculated. DHs were collected at baseline （n = 118）, three months （n = 86）, and 12 months （n = 64）. The accuracy of the conversion process improved with time, with no significant difference between most macronutrients at 12 months. Mean LC n-3 PUFA intake was 441.87 mg at baseline, 521.07 mg at 3 months, and 442.40 mg at 12 months, and was predominantly provided by fish and seafood, followed by meat products. This study allowed for the measurement of LC omega-3 intake, which was previously impossible using the AUSNUT 1999 database.展开更多
基金Supported by the National Key Technology R&D Program in the 11th Five-Year Plan of China (No. 2006BAD09A03-2)the National High-tech Research and Development Program of China(863 Program) (No. 2007AA09Z427)
文摘A gene （NANOC-D6D） encoding a desaturase that removes two hydrogen atoms from fatty acids at delta 6 position was isolated from a cDNA library of Nannochloropsis oculata （Droop） D. J. Hibberd （Eustigmatophyceae）. The unicellular marine microalga N. oculata synthesizes rich long chain polyunsaturated fatty acids （LCPUFAs）, including eicosapentaenoic acid （20：5n-3, EPA）. The deduced protein contains 474 amino acids that fold into 4 trans-membrane domains. The neighbor-joining phylogenetic tree indicates that NANOC-D6D is phylogenetically close to the delta-6 fatty acid desaturase of marine microalgae such as Glossomastix chrysoplasta, Thalassiosira pseudonana, and Phaeodactylum tricornutum. The gene was expressed in Saccharomyces cerevisiae INVScl to verify the substrate specificity of NAN OC-D6D. Our results suggest that the recombinant NANOC-D6D simultaneously desaturates linoleic acid （LA） and a-linolenic acid （ALA）.
文摘An Australian food composition database, AUSNUT1999, does not include long chain omega-3 polyunsaturated fatty acid （LC omega-3 PUFA） data. Measurement of the fatty acid content of diets initially analysed using AUSNUT1999 requires conversion to AUSNUT2007, an updated database inclusive of LC omega-3 PUFA. The aim of this study was to convert clinical trial dietary data from AUSNUT1999 to AUSNUT2007 and measure LC omega-3 PUFA intake. Clinical trial diet history （DH） data was converted from AUSNUTI999 to 2007 using a staged approach. Macronutrient intake from AUSNUTI999 and 2007 were calculated and compared via paired t-tests and Wilcoxon Signed Ranks tests. Mean dietary LC omega-3 PUFA intake and the percentage contribution of food groups to total LC omega-3 PUFA were then calculated. DHs were collected at baseline （n = 118）, three months （n = 86）, and 12 months （n = 64）. The accuracy of the conversion process improved with time, with no significant difference between most macronutrients at 12 months. Mean LC n-3 PUFA intake was 441.87 mg at baseline, 521.07 mg at 3 months, and 442.40 mg at 12 months, and was predominantly provided by fish and seafood, followed by meat products. This study allowed for the measurement of LC omega-3 intake, which was previously impossible using the AUSNUT 1999 database.
