Triglyceride - rich hpoprotein (TRL) remnants have been imphcated by both clinical and laboratory studies in the pathogenesis of atheroselerosis and thrombosis. We use an new immunoseparation method, provided by Japan...Triglyceride - rich hpoprotein (TRL) remnants have been imphcated by both clinical and laboratory studies in the pathogenesis of atheroselerosis and thrombosis. We use an new immunoseparation method, provided by Japan Immunoresearch Laboratories, to determine the remnant - hke particles(RLP) of hpeproteins. Isolation of RLP from serum is achieved using an immunoaffmity gel containing specific anti - human apoA- I and apoB- 100 (JI - H) monoelonal antibodies, where the former recognizes HDL and newly secreted apoA - I - containing chylomicrons, the latter recognizes LDL and majority of VLDL. As a result of the specificity of the JI - H antibody, the majority of the apoB - 48 - containing chylomicrons, and certain apoE - enriched apoB - 100 - containing VLDL axe not recognized. These lipoproteins are not captured by the immunoaffinity gel and are thus isolated in the unbound RLP fraction,which is predominantly made up of TRL having remnant - hke properties. The components of the unbounded fraction axe the remnants of CM and VLDLs, verified by ultracentrifugation and lipoproteins electrophoresis in agarose gels. We established a convenient approach to measure RLP- cholesterol on the Hitachi 7150 biochemical analyser.The within- run and run - to- run imprecision (CV) of the assay was 2.75% and 11.48% respectively. The linear of the RLP- c in our assay was 0 - 180 mg/dl. We chose 30 normal lipids level for the control group ( 15 male & 15 female), the median fasting serum RLP - c concentration was 8.12 ± 4.88 mg/dl. We fred that RLP - c concentration were signficantly correlated with TG, VLDL - c, HDL -c & apoE( r = 0.765,0.511, - 0. 378,0.241 respectively, P < 0.01 ) ; and no signficant correlation was subsequently observed between RLP- c and LDL- c. In our study, the median fasting RLP- c concentration were significantly higher in 91 patients with coronary artery disease (CAD),in 15 patients with non- insulin dependent diabetes meUitus (DM),and in 45 patients undergoing hemodialysis with end - stage retinal diseases than in 30 controls, the median ±SD were 27.61 ± 13.20 mg/dl, 16.64 ± 10.32mg/dl, 24.39 ± 13.87mg/dl vs 8.12 ± 4.88mg/dl( P <0.01). In the hypemiglyceride level of the CAD patients,25 over 46 (54.3%) have severe coronary artery lesions,and 19 over 27(70.4% ) in the normal TG level of the CAD patients as well. We only find significant difference of age and HDL - c ( P = 0. 044, n = 73), but not RLP - c, TC, TG, LDL - c, be-tween the CAD patients with mild and severe coronary artery lesions, which confirmed by angiography;even between the normal TG level of CAD patients with such degree of lesions, only HDL - c ( P = 0.012, n = 27) showed a significant difference. For the retinal diseases patients undergoing hemodialysis, we find the tendency of RLP- c increasing and HLD - c decreasing according to the time of hemodialysis. In conclusion, RLP - c level is strongly associated with CAD & DM independent of LDL - c, and associated with coronary artery lesions in the CAD patients with normal triglycerides. It can be a new lipid parameter to evaluate the hemodialysis.展开更多
目的通过对比分析血脂浓度在冠状动脉粥样硬化性心脏病(以下简称冠心病)不同亚组患者中的浓度,探讨残粒脂蛋白胆固醇(residual lipoprotein-cholesterol,RLP-C)与急性心肌梗死(acute myocardial infarction,AMI)的相关性。方法选取2020...目的通过对比分析血脂浓度在冠状动脉粥样硬化性心脏病(以下简称冠心病)不同亚组患者中的浓度,探讨残粒脂蛋白胆固醇(residual lipoprotein-cholesterol,RLP-C)与急性心肌梗死(acute myocardial infarction,AMI)的相关性。方法选取2020年1月至2021年1月首都医科大学附属北京朝阳医院心内科收治的冠心病患者为研究对象,将其中266例AMI患者作为病例组(AMI组),267例非AMI患者为对照组(非AMI组),开展病例对照研究。基于公式计算出RLP-C指标和血浆致动脉粥样硬化指数(atherogenic index of plasma,AIP)。通过单因素分析及多因素Logistic回归模型,分析RLP-C浓度与AMI的关系。结果单因素分析结果显示,AMI组患者RLP-C浓度显著高于非AMI组患者,组间差异有统计学意义(P<0.05)。多因素Logistic回归分析显示,在控制了其他因素的干扰作用后,RLP-C仍然为AMI的独立危险因素(P<0.05),RLP-C浓度升高的患者,AMI的危险性增大,并且在诸多影响因素中,RLP-C与AMI关系最为密切(OR=3.093,95%CI:1.658~5.771)。结论血清RLP-C浓度与急性心肌梗死有关,RLP-C浓度升高的患者发生AMI的风险增大。展开更多
文摘Triglyceride - rich hpoprotein (TRL) remnants have been imphcated by both clinical and laboratory studies in the pathogenesis of atheroselerosis and thrombosis. We use an new immunoseparation method, provided by Japan Immunoresearch Laboratories, to determine the remnant - hke particles(RLP) of hpeproteins. Isolation of RLP from serum is achieved using an immunoaffmity gel containing specific anti - human apoA- I and apoB- 100 (JI - H) monoelonal antibodies, where the former recognizes HDL and newly secreted apoA - I - containing chylomicrons, the latter recognizes LDL and majority of VLDL. As a result of the specificity of the JI - H antibody, the majority of the apoB - 48 - containing chylomicrons, and certain apoE - enriched apoB - 100 - containing VLDL axe not recognized. These lipoproteins are not captured by the immunoaffinity gel and are thus isolated in the unbound RLP fraction,which is predominantly made up of TRL having remnant - hke properties. The components of the unbounded fraction axe the remnants of CM and VLDLs, verified by ultracentrifugation and lipoproteins electrophoresis in agarose gels. We established a convenient approach to measure RLP- cholesterol on the Hitachi 7150 biochemical analyser.The within- run and run - to- run imprecision (CV) of the assay was 2.75% and 11.48% respectively. The linear of the RLP- c in our assay was 0 - 180 mg/dl. We chose 30 normal lipids level for the control group ( 15 male & 15 female), the median fasting serum RLP - c concentration was 8.12 ± 4.88 mg/dl. We fred that RLP - c concentration were signficantly correlated with TG, VLDL - c, HDL -c & apoE( r = 0.765,0.511, - 0. 378,0.241 respectively, P < 0.01 ) ; and no signficant correlation was subsequently observed between RLP- c and LDL- c. In our study, the median fasting RLP- c concentration were significantly higher in 91 patients with coronary artery disease (CAD),in 15 patients with non- insulin dependent diabetes meUitus (DM),and in 45 patients undergoing hemodialysis with end - stage retinal diseases than in 30 controls, the median ±SD were 27.61 ± 13.20 mg/dl, 16.64 ± 10.32mg/dl, 24.39 ± 13.87mg/dl vs 8.12 ± 4.88mg/dl( P <0.01). In the hypemiglyceride level of the CAD patients,25 over 46 (54.3%) have severe coronary artery lesions,and 19 over 27(70.4% ) in the normal TG level of the CAD patients as well. We only find significant difference of age and HDL - c ( P = 0. 044, n = 73), but not RLP - c, TC, TG, LDL - c, be-tween the CAD patients with mild and severe coronary artery lesions, which confirmed by angiography;even between the normal TG level of CAD patients with such degree of lesions, only HDL - c ( P = 0.012, n = 27) showed a significant difference. For the retinal diseases patients undergoing hemodialysis, we find the tendency of RLP- c increasing and HLD - c decreasing according to the time of hemodialysis. In conclusion, RLP - c level is strongly associated with CAD & DM independent of LDL - c, and associated with coronary artery lesions in the CAD patients with normal triglycerides. It can be a new lipid parameter to evaluate the hemodialysis.
文摘目的通过对比分析血脂浓度在冠状动脉粥样硬化性心脏病(以下简称冠心病)不同亚组患者中的浓度,探讨残粒脂蛋白胆固醇(residual lipoprotein-cholesterol,RLP-C)与急性心肌梗死(acute myocardial infarction,AMI)的相关性。方法选取2020年1月至2021年1月首都医科大学附属北京朝阳医院心内科收治的冠心病患者为研究对象,将其中266例AMI患者作为病例组(AMI组),267例非AMI患者为对照组(非AMI组),开展病例对照研究。基于公式计算出RLP-C指标和血浆致动脉粥样硬化指数(atherogenic index of plasma,AIP)。通过单因素分析及多因素Logistic回归模型,分析RLP-C浓度与AMI的关系。结果单因素分析结果显示,AMI组患者RLP-C浓度显著高于非AMI组患者,组间差异有统计学意义(P<0.05)。多因素Logistic回归分析显示,在控制了其他因素的干扰作用后,RLP-C仍然为AMI的独立危险因素(P<0.05),RLP-C浓度升高的患者,AMI的危险性增大,并且在诸多影响因素中,RLP-C与AMI关系最为密切(OR=3.093,95%CI:1.658~5.771)。结论血清RLP-C浓度与急性心肌梗死有关,RLP-C浓度升高的患者发生AMI的风险增大。
