目的分析自然早产胎盘中细胞凋亡抑制因子生存素(survivin)表达水平及其定量检测在自然早产中的诊断价值。方法选择2015年12月‐2016年12月福田医院住院部分娩的60例胎盘,其中自然早产30例(观察组),足月产30例(对照组)。采集胎盘,对两...目的分析自然早产胎盘中细胞凋亡抑制因子生存素(survivin)表达水平及其定量检测在自然早产中的诊断价值。方法选择2015年12月‐2016年12月福田医院住院部分娩的60例胎盘,其中自然早产30例(观察组),足月产30例(对照组)。采集胎盘,对两组分别采用细胞凋亡抑制因子survivin免疫检测和survivin m RNA定量测定逆转录-聚合酶链反应(RT-PCR)法并进行比较。结果观察组胎盘survivin因子阳性表达率为26.67%(8/30),survivin-m RNA基因阳性表达率为16.67%(5/30),对照组分别为53.33%(16/30)、50.00%(15/30),差异具有统计学意义(P<0.05)。观察组胎盘survivin因子阳性细胞百分比为(57.10±4.82)%,survivin-m RNA相对表达量(0.68±0.23),对照组分别为(72.06±7.59)%、(2.09±0.47),差异具有统计学意义(t分别为11.102、4.910,P<0.05)。结论适时监测诱发孕妇早产的survivin因子,及早治疗干预,有助于避免早产的发生,提高新生儿的生存质量。展开更多
Objective: This study was conducted to determine whether there is a relationship between the concentration of fetal cell-free DNA in maternal serum and the duration of pregnancy in women who are at high risk for prete...Objective: This study was conducted to determine whether there is a relationship between the concentration of fetal cell-free DNA in maternal serum and the duration of pregnancy in women who are at high risk for preterm delivery because of either preterm labor or preterm premature rupture of the membranes. Study design: Sera were collected and frozen from 71 women with a male fetus. Maternal serum fetal cell-free DNA concentration was measured with the use of real-time polymerase chain reaction amplification of DYS1. Fetal cell-free DNA concentrations were converted to multiples of the median. The following groups were studied: group 1: women with preterm labor and intact membranes who were delivered at ≥ 36 weeks of gestation (n = 21); group 2: women with preterm labor who were delivered at < 36 weeks of gestation (n = 29); and group 3: women with preterm premature rupture of the membranes in labor (n = 20) or not in labor (n = 1) who were delivered prematurely ( < 36 weeks of gestation). Kaplan-Meier and Cox regression analyses were used to analyze the relationship between fetal cell-free DNA concentrations and the likelihood of preterm delivery. Results: A cut-off value for fetal cell-free DNA of 1.82 multiples of the median was chosen for analysis. The cumulative rate of early preterm delivery ( < 30 weeks of gestation) was significantly higher for women with fetal cell-free DNA concentrations of ≥ 1.82 multiples of the median than those with fetal cell-free DNA concentrations below this cut-off (45% [95% CI, 36% - 74% ] vs 18% [95% CI, 11% - 25% ]; P = .008]. The cumulative rate of preterm delivery ( < 36 weeks of gestation) was also significantly higher at ≥ 1.82 multiples of the median (73% [95% CI, 52% - 93% ] vs 66% [95% CI, 54% - 79% ]; P = .02). After adjustment for covariates, Cox analysis showed that fetal cell-free DNA at ≥ 1.82 multiples of the mechanisms of disease that are associated with a mean hazard rate of delivery of 1.57 (P = .005). Conclusion: High concentrations of fetal cell-free DNA in maternal serum are associated with an increased risk of spontaneous preterm delivery. This observation may have implications for the understanding of the mechanisms of disease that is associated with preterm labor.展开更多
文摘目的分析自然早产胎盘中细胞凋亡抑制因子生存素(survivin)表达水平及其定量检测在自然早产中的诊断价值。方法选择2015年12月‐2016年12月福田医院住院部分娩的60例胎盘,其中自然早产30例(观察组),足月产30例(对照组)。采集胎盘,对两组分别采用细胞凋亡抑制因子survivin免疫检测和survivin m RNA定量测定逆转录-聚合酶链反应(RT-PCR)法并进行比较。结果观察组胎盘survivin因子阳性表达率为26.67%(8/30),survivin-m RNA基因阳性表达率为16.67%(5/30),对照组分别为53.33%(16/30)、50.00%(15/30),差异具有统计学意义(P<0.05)。观察组胎盘survivin因子阳性细胞百分比为(57.10±4.82)%,survivin-m RNA相对表达量(0.68±0.23),对照组分别为(72.06±7.59)%、(2.09±0.47),差异具有统计学意义(t分别为11.102、4.910,P<0.05)。结论适时监测诱发孕妇早产的survivin因子,及早治疗干预,有助于避免早产的发生,提高新生儿的生存质量。
文摘Objective: This study was conducted to determine whether there is a relationship between the concentration of fetal cell-free DNA in maternal serum and the duration of pregnancy in women who are at high risk for preterm delivery because of either preterm labor or preterm premature rupture of the membranes. Study design: Sera were collected and frozen from 71 women with a male fetus. Maternal serum fetal cell-free DNA concentration was measured with the use of real-time polymerase chain reaction amplification of DYS1. Fetal cell-free DNA concentrations were converted to multiples of the median. The following groups were studied: group 1: women with preterm labor and intact membranes who were delivered at ≥ 36 weeks of gestation (n = 21); group 2: women with preterm labor who were delivered at < 36 weeks of gestation (n = 29); and group 3: women with preterm premature rupture of the membranes in labor (n = 20) or not in labor (n = 1) who were delivered prematurely ( < 36 weeks of gestation). Kaplan-Meier and Cox regression analyses were used to analyze the relationship between fetal cell-free DNA concentrations and the likelihood of preterm delivery. Results: A cut-off value for fetal cell-free DNA of 1.82 multiples of the median was chosen for analysis. The cumulative rate of early preterm delivery ( < 30 weeks of gestation) was significantly higher for women with fetal cell-free DNA concentrations of ≥ 1.82 multiples of the median than those with fetal cell-free DNA concentrations below this cut-off (45% [95% CI, 36% - 74% ] vs 18% [95% CI, 11% - 25% ]; P = .008]. The cumulative rate of preterm delivery ( < 36 weeks of gestation) was also significantly higher at ≥ 1.82 multiples of the median (73% [95% CI, 52% - 93% ] vs 66% [95% CI, 54% - 79% ]; P = .02). After adjustment for covariates, Cox analysis showed that fetal cell-free DNA at ≥ 1.82 multiples of the mechanisms of disease that are associated with a mean hazard rate of delivery of 1.57 (P = .005). Conclusion: High concentrations of fetal cell-free DNA in maternal serum are associated with an increased risk of spontaneous preterm delivery. This observation may have implications for the understanding of the mechanisms of disease that is associated with preterm labor.
