目的研究二甲双胍(metformin,Met)通过抑制NF-κBNLRP3通路改善草酸钙(Calcium Oxalate,CaOx)诱导人肾小管上皮细胞(HK-2)损伤。方法分别随机将人肾小管上皮细胞分成对照组、实验组(CaOx+HK-2)、高浓度Met组(CaOx+HK-2+1.2 mM Met)、低...目的研究二甲双胍(metformin,Met)通过抑制NF-κBNLRP3通路改善草酸钙(Calcium Oxalate,CaOx)诱导人肾小管上皮细胞(HK-2)损伤。方法分别随机将人肾小管上皮细胞分成对照组、实验组(CaOx+HK-2)、高浓度Met组(CaOx+HK-2+1.2 mM Met)、低浓度Met组(CaOx+HK-2+0.80 mM Met)、通路干预组(CaOx+HK-2+PDTC)五组。各组细胞培养24 h,采用酶联免疫吸附测定(Elisa)法检测细胞上清液中炎症因子(IL-6、IL-18、IL-1β),采用逆转录-聚合酶链反应(RT-PCR)法检测细胞中NF-κB、NLRP3、骨桥蛋白(OPN)mRNA。结果各组间细胞上清液IL-6、IL-18、IL-1β含量相比差异均有统计学意义(P均<0.05);各组间细胞中NF-κBmRNA表达量、NLRP3mRNA表达量、OPNmRNA表达量相比差异均有统计学意义(P均<0.05)。结论1.20 mmol/L或0.80 mmol/L Met可以通过抑制NF-κB/NLRP3通路调控下游的炎症相关蛋白及黏附蛋白表达,继而改善CaOx诱导人肾小管上皮细胞损伤及减少草酸钙肾结石形成。展开更多
In this paper, the mimetic biomineralization of CaC2O4 in lecithin water(PC H2O) ordered systems has been discussed. First, the effect of the aggregation state of PC aqueous solutions on the crystal structure of CaC2O...In this paper, the mimetic biomineralization of CaC2O4 in lecithin water(PC H2O) ordered systems has been discussed. First, the effect of the aggregation state of PC aqueous solutions on the crystal structure of CaC2O4 has been studied. In all experiments, the only precipitated crystal forms obtained are calcium oxalate monohydrate(COM) and calcium oxalate dihydrate(COD). It have been shown that the relative amount of COD in PC H2O systems is much higher than that of in H2O system, and furthermore the aggregation state of PC influences the composition of coprecipitations to some extent. When PC distributing in aqueous solution as single molecules, it acts as inhibitor of COM , and then favors the formation of COD. While PC in micelle existence, the relative amount of COM increases , but is still lower than that of in H2O system . And when PC aggregates as vesicles, COD forms predominantly. Second, the morphology of CaC2O4 obtained in different reaction systems under different conditions has been also investigated. In H2O system, we observed thin, plate like crystals with serrated edges underISL(low ionic strength) condition and oriented clusters under ISH(high ionic strength) condition. Crystals with two layers arrangement were obtained in micelleordered PC H2O system under ISL condition. And in vesicle ordered PC H2O system, clusters with butterfly like shape were observed. The results obtained revealed a complex dependence of calcium oxalate formation,crystal growth, structure and morphology on the aggregation state of PC H2O systems.展开更多
文摘目的研究二甲双胍(metformin,Met)通过抑制NF-κBNLRP3通路改善草酸钙(Calcium Oxalate,CaOx)诱导人肾小管上皮细胞(HK-2)损伤。方法分别随机将人肾小管上皮细胞分成对照组、实验组(CaOx+HK-2)、高浓度Met组(CaOx+HK-2+1.2 mM Met)、低浓度Met组(CaOx+HK-2+0.80 mM Met)、通路干预组(CaOx+HK-2+PDTC)五组。各组细胞培养24 h,采用酶联免疫吸附测定(Elisa)法检测细胞上清液中炎症因子(IL-6、IL-18、IL-1β),采用逆转录-聚合酶链反应(RT-PCR)法检测细胞中NF-κB、NLRP3、骨桥蛋白(OPN)mRNA。结果各组间细胞上清液IL-6、IL-18、IL-1β含量相比差异均有统计学意义(P均<0.05);各组间细胞中NF-κBmRNA表达量、NLRP3mRNA表达量、OPNmRNA表达量相比差异均有统计学意义(P均<0.05)。结论1.20 mmol/L或0.80 mmol/L Met可以通过抑制NF-κB/NLRP3通路调控下游的炎症相关蛋白及黏附蛋白表达,继而改善CaOx诱导人肾小管上皮细胞损伤及减少草酸钙肾结石形成。
文摘In this paper, the mimetic biomineralization of CaC2O4 in lecithin water(PC H2O) ordered systems has been discussed. First, the effect of the aggregation state of PC aqueous solutions on the crystal structure of CaC2O4 has been studied. In all experiments, the only precipitated crystal forms obtained are calcium oxalate monohydrate(COM) and calcium oxalate dihydrate(COD). It have been shown that the relative amount of COD in PC H2O systems is much higher than that of in H2O system, and furthermore the aggregation state of PC influences the composition of coprecipitations to some extent. When PC distributing in aqueous solution as single molecules, it acts as inhibitor of COM , and then favors the formation of COD. While PC in micelle existence, the relative amount of COM increases , but is still lower than that of in H2O system . And when PC aggregates as vesicles, COD forms predominantly. Second, the morphology of CaC2O4 obtained in different reaction systems under different conditions has been also investigated. In H2O system, we observed thin, plate like crystals with serrated edges underISL(low ionic strength) condition and oriented clusters under ISH(high ionic strength) condition. Crystals with two layers arrangement were obtained in micelleordered PC H2O system under ISL condition. And in vesicle ordered PC H2O system, clusters with butterfly like shape were observed. The results obtained revealed a complex dependence of calcium oxalate formation,crystal growth, structure and morphology on the aggregation state of PC H2O systems.
