药物分子从药物载体中的释放行为与载体的结构有密切关系.本实验中采用丙烯酰胺和丙烯酸等材料,运用水相沉淀的方法,制备了4种不同单体配比的聚丙烯酰胺-丙烯酸(P(Am-co-Ac))共聚物水凝胶.运用红外分析方法对P(Am-co-Ac)组成进行表征....药物分子从药物载体中的释放行为与载体的结构有密切关系.本实验中采用丙烯酰胺和丙烯酸等材料,运用水相沉淀的方法,制备了4种不同单体配比的聚丙烯酰胺-丙烯酸(P(Am-co-Ac))共聚物水凝胶.运用红外分析方法对P(Am-co-Ac)组成进行表征.使用荧光漂白恢复法(FRAP,fluorescence recovery after photobleaching)观察荧光素FITC标记的牛血清白蛋白(BSA)在聚丙烯酰胺-丙烯酸中的扩散行为,并以激光共聚焦显微镜进行实时成像.实验表明,FITC-BSA在不同单体配比的共聚物中的扩散系数是不同的.通过调节聚合物中单体的配比能够达到控制蛋白释放速率的作用,从而为调控蛋白和多肽类药物的缓控释放提供了可能性.展开更多
Survival of the plant-growth promoting rhizobacteria （PGPR） strain Pseudomonasfluorescens 92 in two batches ofbiosolids and soil amended with them and functional and structural shifts occurring in the resident bacte...Survival of the plant-growth promoting rhizobacteria （PGPR） strain Pseudomonasfluorescens 92 in two batches ofbiosolids and soil amended with them and functional and structural shifts occurring in the resident bacterial communities were assessed. Viability of the rifampicin resistant gfp-tagged P. fluorescens 92RTcgfp, inoculated in microcosms consisting of biosolids, soil amended with biosolids and soil, drastically decreased 3-7 days after inoculation in treatments containing biosolids and soil amended with them. The PGPR counts in biosolids were always lower than in soil throughout the experiment whereas, in soil amended with biosolids, were similar or higher depending on the batch of biosolid. Analysis of the community-level physiological profiling （CLPP） revealed a strong impact of the strain on the metabolic activities, as seen in a general uniformity of the inoculated microcosms profiles. According to single-strand conformation polymorphism （SSCP） analysis, the presence of the PGPR had no effect on the resident soil and biosolid eubacterial population, whereas it induced a transient shift in the composition of the resident pseudomonads. In conclusion, biosolids themselves are not suitable as inoculum vehicle for the PGPR nevertheless, their combined incorporation into soil increases the inoculum survival and buffers the competition effects with the resident communities.展开更多
文摘药物分子从药物载体中的释放行为与载体的结构有密切关系.本实验中采用丙烯酰胺和丙烯酸等材料,运用水相沉淀的方法,制备了4种不同单体配比的聚丙烯酰胺-丙烯酸(P(Am-co-Ac))共聚物水凝胶.运用红外分析方法对P(Am-co-Ac)组成进行表征.使用荧光漂白恢复法(FRAP,fluorescence recovery after photobleaching)观察荧光素FITC标记的牛血清白蛋白(BSA)在聚丙烯酰胺-丙烯酸中的扩散行为,并以激光共聚焦显微镜进行实时成像.实验表明,FITC-BSA在不同单体配比的共聚物中的扩散系数是不同的.通过调节聚合物中单体的配比能够达到控制蛋白释放速率的作用,从而为调控蛋白和多肽类药物的缓控释放提供了可能性.
文摘Survival of the plant-growth promoting rhizobacteria （PGPR） strain Pseudomonasfluorescens 92 in two batches ofbiosolids and soil amended with them and functional and structural shifts occurring in the resident bacterial communities were assessed. Viability of the rifampicin resistant gfp-tagged P. fluorescens 92RTcgfp, inoculated in microcosms consisting of biosolids, soil amended with biosolids and soil, drastically decreased 3-7 days after inoculation in treatments containing biosolids and soil amended with them. The PGPR counts in biosolids were always lower than in soil throughout the experiment whereas, in soil amended with biosolids, were similar or higher depending on the batch of biosolid. Analysis of the community-level physiological profiling （CLPP） revealed a strong impact of the strain on the metabolic activities, as seen in a general uniformity of the inoculated microcosms profiles. According to single-strand conformation polymorphism （SSCP） analysis, the presence of the PGPR had no effect on the resident soil and biosolid eubacterial population, whereas it induced a transient shift in the composition of the resident pseudomonads. In conclusion, biosolids themselves are not suitable as inoculum vehicle for the PGPR nevertheless, their combined incorporation into soil increases the inoculum survival and buffers the competition effects with the resident communities.
