This article presents the designing and fabrication of a microfluoidic chip with an integrated supported-liquid-membrane extraction-back-extraction and chip-based capillary electrophoresis system(SLMEBE-CCE).A 3-dimen...This article presents the designing and fabrication of a microfluoidic chip with an integrated supported-liquid-membrane extraction-back-extraction and chip-based capillary electrophoresis system(SLMEBE-CCE).A 3-dimensional channel network for the SLMEBE-CCE system was fabricated with a four-layer glass/PTFE-membrane hybrid structure. With a gravity pump for sample and back-extraction solution delivery and a multi-channel high-voltage supply for CCE separation,the microchip with the coupled SLMEBE-CCE system was demonstrated by the pre-concentration,separation and detection of a mixed solution of fluorescein and butyl-rhodamine B.Compared to chip-based CCE without SLMEBE,the developed SLMEBE-CCE system produced a 7-fold increase in the signal of the model analyte of fluorescein and a 4-fold decrease in the signal of the model co-existing compound of butyl-rhodamine B.展开更多
The controlling step and the extraction reaction rate equation of zinc extraction from Zn（II）-NH3 solution by using a newly synthesized organic compound, 2-acetyl-3-oxo-dithiobutyric acid-myristyl ester as the zinc ...The controlling step and the extraction reaction rate equation of zinc extraction from Zn（II）-NH3 solution by using a newly synthesized organic compound, 2-acetyl-3-oxo-dithiobutyric acid-myristyl ester as the zinc extractant, were clarified. The effects of agitation speed, specific interfacial area, temperature, extractant concentration and Zn ion concentration on the extraction rate are studied in constant interfacial area cell. The results show that the extraction rate depends on interfacial chemical reaction and diffusion by using this new extraetant to extract zinc, and the apparent activation energy of this extraction reaction is measured as 28.2 kJ/mol, which demonstrates that the extraction reaction is controlled by the mixed-controlled reaction rate. The apparent reaction orders a and b are measured as 1 and 0.38, and the constant k0 is 138.70. So, when extraction conditions are controlled as [HR]=20%-50%, T=0-30℃, N=120-177 r/min and S=72.6-127.5 m-1, the solvent extraction reaction rate can be depicted as v/（mol . m-2 . s-1 ） = 138.7. exp（ - 28 206/8.314T ）. [Zn 2＋ ]r ·[HR ]o0.38.展开更多
文摘This article presents the designing and fabrication of a microfluoidic chip with an integrated supported-liquid-membrane extraction-back-extraction and chip-based capillary electrophoresis system(SLMEBE-CCE).A 3-dimensional channel network for the SLMEBE-CCE system was fabricated with a four-layer glass/PTFE-membrane hybrid structure. With a gravity pump for sample and back-extraction solution delivery and a multi-channel high-voltage supply for CCE separation,the microchip with the coupled SLMEBE-CCE system was demonstrated by the pre-concentration,separation and detection of a mixed solution of fluorescein and butyl-rhodamine B.Compared to chip-based CCE without SLMEBE,the developed SLMEBE-CCE system produced a 7-fold increase in the signal of the model analyte of fluorescein and a 4-fold decrease in the signal of the model co-existing compound of butyl-rhodamine B.
基金Foundation item： Project（51174240） supported by the National Natural Science Foundation of China Project（2006BA02B04-4-2） supported by the National Eleventh Five-Year Research Program of China Project（20100908） supported by Scientific and Industrial Research Organisation of Guangdong Province, China
文摘The controlling step and the extraction reaction rate equation of zinc extraction from Zn（II）-NH3 solution by using a newly synthesized organic compound, 2-acetyl-3-oxo-dithiobutyric acid-myristyl ester as the zinc extractant, were clarified. The effects of agitation speed, specific interfacial area, temperature, extractant concentration and Zn ion concentration on the extraction rate are studied in constant interfacial area cell. The results show that the extraction rate depends on interfacial chemical reaction and diffusion by using this new extraetant to extract zinc, and the apparent activation energy of this extraction reaction is measured as 28.2 kJ/mol, which demonstrates that the extraction reaction is controlled by the mixed-controlled reaction rate. The apparent reaction orders a and b are measured as 1 and 0.38, and the constant k0 is 138.70. So, when extraction conditions are controlled as [HR]=20%-50%, T=0-30℃, N=120-177 r/min and S=72.6-127.5 m-1, the solvent extraction reaction rate can be depicted as v/（mol . m-2 . s-1 ） = 138.7. exp（ - 28 206/8.314T ）. [Zn 2＋ ]r ·[HR ]o0.38.
