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雨生红球藻(Haematococcus pluvialis)破壁方法对虾青素提取率的影响 被引量:24
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作者 周湘池 刘必谦 +1 位作者 曾庆国 蒋霞敏 《海洋与湖沼》 CAS CSCD 北大核心 2006年第5期424-429,共6页
采用匀浆法、冻融温差法、超声波法、直接研磨法和低温研磨法等5种破壁方法,研究了雨生红球藻提取虾青素时破壁方法对虾青素提取率的影响。结果表明,对雨生红球藻进行破壁是必要的。其中匀浆法、冻融温差法、超声波法、直接研磨法和低... 采用匀浆法、冻融温差法、超声波法、直接研磨法和低温研磨法等5种破壁方法,研究了雨生红球藻提取虾青素时破壁方法对虾青素提取率的影响。结果表明,对雨生红球藻进行破壁是必要的。其中匀浆法、冻融温差法、超声波法、直接研磨法和低温研磨法等的最佳破壁条件为:匀浆法破壁时间22min,水为介质;冻融温差法破壁温度为-70℃,时间为12h,冻融2次,水为介质;超声功率400W,每次超声时间5s,共超声25min;直接研磨法研磨时间1min;加液氮低温研磨法破壁2次,每次时间0·5min;虾青素的提取率依次为0·76%、0·93%、1·03%、1·51%和3·21%。匀浆法和超声波法破壁由于要使用溶剂或介质,对后续的虾青素提取和分离会有影响,而直接研磨法在破壁过程中产生高温,降低了虾青素的生理活性,所以它们都不是雨生红球藻破壁的最佳方法。加液氮低温研磨法在破壁过程中不添加化学试剂,不产生污染,能最大限度地保留虾青素的生理活性,是所选方法中最好的一种。 展开更多
关键词 雨生红球藻 青素 细胞破壁 虾青素提取
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雨生红球藻中虾青素提取方法研究现状 被引量:14
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作者 孟春晓 高政权 +2 位作者 王依涛 罗韬 叶乃好 《水产科学》 CAS 北大核心 2010年第12期745-748,共4页
关键词 雨生红球藻 虾青素提取
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皂化处理对中华管鞭虾虾青素提取物的虾青素组成和体外抗氧化性的影响 被引量:2
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作者 贾喆 张肖瑕 +2 位作者 刘欣妍 许艳 宋茹 《食品工业科技》 CAS 北大核心 2021年第13期80-87,共8页
采用低温皂化中华管鞭虾的虾青素提取物,比较皂化前后虾青素总浓度、抗氧化活性、游离型和酯型虾青素相对浓度以及虾青素光学异构体组成变化。结果表明:适当皂化处理可显著提高虾青素提取物的总虾青素浓度,皂化时间对虾青素提取物的抗... 采用低温皂化中华管鞭虾的虾青素提取物,比较皂化前后虾青素总浓度、抗氧化活性、游离型和酯型虾青素相对浓度以及虾青素光学异构体组成变化。结果表明:适当皂化处理可显著提高虾青素提取物的总虾青素浓度,皂化时间对虾青素提取物的抗氧性有显著影响(P<0.05),其中皂化2 h时对DPPH自由基清除率达到25.85%,显著高于未皂化的15.16%,虾青素提取物对羟自由基和总抗氧化能力则在皂化6 h时分别达到3.17和0.62 U/μg。高效液相分析结果显示:皂化2 h时虾青素提取物中酯型虾青素已经大部分转化为游离型虾青素,而延长皂化时间会造成游离型虾青素发生不同程度的降解。未皂化的虾青素提取物光学异构体以3S,3’S型为主,经过皂化处理后光学异构体3S,3’S:3S,3’R:3R,3’R比值保持在2.4:1.5~1.7:1.0之间。此外,研究还发现皂化后虾青素提取物中三种光学异构体含量均与羟自由基清除能力呈现较好的线性相关性(R>0.784)。综上,皂化后中华管鞭虾的虾青素提取物抗氧化性变化与游离型和酯型虾青素组成变化及光学异构体组成变化有关。 展开更多
关键词 中华管鞭 虾青素提取 皂化 抗氧化 异构体
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探究乙醇-丙酮不同比例对雨生红球藻中虾青素提取速率的影响 被引量:1
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作者 李小慧 邹宁 +1 位作者 孙东红 张军 《安徽农业科学》 CAS 2015年第28期7-8,12,共3页
[目的]探究了用不同配比的有机溶剂浸提法提取雨生红球藻中虾青素的效果。[方法]在乙醇和丙酮的不同比例下(1∶0、1∶1、1∶2、2∶1、0∶1,V/V),比较雨生红球藻提取得到虾青素的含量,并且用分光度计法检测OD值,计算出虾青素的含量。[结... [目的]探究了用不同配比的有机溶剂浸提法提取雨生红球藻中虾青素的效果。[方法]在乙醇和丙酮的不同比例下(1∶0、1∶1、1∶2、2∶1、0∶1,V/V),比较雨生红球藻提取得到虾青素的含量,并且用分光度计法检测OD值,计算出虾青素的含量。[结果]在试验条件下,乙醇/丙酮为1∶2(V/V)时虾青素的的提取率为15.59 mg/g,丙酮的提取率为15.06 mg/g,乙醇/丙酮为1∶1(V/V)时提取率为12.72mg/g,乙醇/丙酮为2∶1(V/V)时提取率为12.47 mg/g,乙醇的提取率为10.11 mg/g。[结论]乙醇/丙酮为1∶2(V/V)时提取率最高,其次是丙酮。 展开更多
关键词 雨生红球藻 乙醇-丙酮不同比例 提取青素 有机溶剂法
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高温湿热酸法破壁提取法夫酵母胞内虾青素 被引量:16
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作者 卢宝驹 肖安风 +3 位作者 李利君 倪辉 蔡慧农 苏文金 《生物工程学报》 CAS CSCD 北大核心 2008年第7期1285-1292,共8页
法夫酵母是一种能积累虾青素的红酵母,对其进行破壁是当前虾青素工业化提取生产的瓶颈工艺。研究在高温湿热条件下,低浓度盐酸对法夫酵母破壁而提取其胞内虾青素的工艺。探讨了不同破壁温度、盐酸浓度、液料比与破壁处理时间等因素对法... 法夫酵母是一种能积累虾青素的红酵母,对其进行破壁是当前虾青素工业化提取生产的瓶颈工艺。研究在高温湿热条件下,低浓度盐酸对法夫酵母破壁而提取其胞内虾青素的工艺。探讨了不同破壁温度、盐酸浓度、液料比与破壁处理时间等因素对法夫酵母破壁后虾青素及类胡萝卜素提取率的影响,确定了高温湿热酸法破壁提取虾青素的最佳条件为:饱和蒸汽压力0.1MPa(121°C),盐酸浓度0.5mol/L,液料比(V/W)30mL/g,破壁时间2min。在最佳条件下进行中试放大实验,可得到虾青素与类胡萝卜素的提取率分别为:(84.8±3.2)%,(93.3±2)%。经优化后的新破壁工艺安全高效,不会有毒性残留,具有良好的工业应用前景。 展开更多
关键词 法夫酵母 细胞破壁 虾青素提取 高温湿热 盐酸
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Optimization of acidic extraction of astaxanthin from Phaffia rhodozyma 被引量:12
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作者 Hui NI Qi-he CHEN +2 位作者 Guo-qing HE Guang-bin WU Yuan-fan YANG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2008年第1期51-59,共9页
Optimization of a process for extracting astaxanthin from Phaffia rhodozyma by acidic method was investigated, regarding several extraction factors such as acids, organic solvents, temperature and time. Fractional fac... Optimization of a process for extracting astaxanthin from Phaffia rhodozyma by acidic method was investigated, regarding several extraction factors such as acids, organic solvents, temperature and time. Fractional factorial design, central composite design and response surface methodology were used to derive a statistically optimal model, which corresponded to the following optimal condition: concentration of lactic acid at 5.55 mol/L, ratio of ethanol to yeast dry weight at 20.25 ml/g, temperature for cell-disruption at 30 ℃, and extraction time for 3 min. Under this condition, astaxanthin and the total carotenoids could be extracted in amounts of 1294.7 μg/g and 1516.0 μg/g, respectively. This acidic method has advantages such as high extraction efficiency, low chemical toxicity and no special requirement of instruments. Therefore, it might be a more feasible and practical method for industrial practice. 展开更多
关键词 ASTAXANTHIN Phaffia rhodozyma EXTRACTING OPTIMIZATION Acidic method
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Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing 被引量:6
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作者 尹春华 杨淑珍 +1 位作者 刘晓璐 闫海 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2013年第7期776-780,共5页
method of extracting astaxanthin from Phaffia rhodozyma with various solvents after acid washing was investigated. The extraction efficiency was distinctly increased after acid washing of P. rhodozyma cells. When the ... method of extracting astaxanthin from Phaffia rhodozyma with various solvents after acid washing was investigated. The extraction efficiency was distinctly increased after acid washing of P. rhodozyma cells. When the concentration of HCl was 0.4 mol.L^-1, the highest extraction efficiency of astaxanthin was achieved which was about three times higher than the control. Acetone or benzene as single polar or non-polar solvent was the most ef- fective solvent in our research. With a combination of isopropanol and n-hexane (volume ratio of 2 : 1), the maxi- mal extraction efficiency was achieved, approximately 60% higher than that obtained with a single solvent. The liquid-solid ratio and the extracting time were also optimized. Under the optimum extraction conditions, the extraction yield of astaxanthin exceeded 98%. 展开更多
关键词 ASTAXANTHIN Phaffia rhodozyma acid wash EXTRACTION SOLVENT
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Extraction of Astaxanthin from Euphausia pacific Using Subcritical 1,1,1,2-tetrafluoroethane 被引量:5
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作者 HAN Yuqian MA Qinchuan +1 位作者 WANG Lan XUE Changhu 《Journal of Ocean University of China》 SCIE CAS 2012年第4期562-568,共7页
Euphausia pacific is an important source of natural astaxanthin. Studies were carried out to assess the extractability of astaxanthin from E. pacific using subcritical 1, 1, 1,2-tetrafluoroethane (R134a). To examine... Euphausia pacific is an important source of natural astaxanthin. Studies were carried out to assess the extractability of astaxanthin from E. pacific using subcritical 1, 1, 1,2-tetrafluoroethane (R134a). To examine the effects of multiple process variables on the extraction yield, astaxanthin was extracted under various conditions of pressure (30-150bar), temperature (303-343 K), time (10-50rain), flow rate (2-10gmin-1), moisture content (5.5%-63.61%), and particle size (0.25-0.109mm). The results showed that the extraction yield increased with temperature, pressure, time and flow rate, but decreased with moisture content and particle size. A maximum yield of 87.74% was obtained under conditions of 100bar, 333K, and 30min with a flow rate of 6gmin-1 and a moisture content of 5.5%. The substantial astaxanthin yield obtained under low-pressure conditions demonstrates that subcritical R134a is a good alternative to CO2 for extraction of astaxanthin from E. pacific. 展开更多
关键词 subcritical R134a astaxanthin extraction Euphausia pacific
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Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically 被引量:4
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作者 李夜光 苗凤萍 +3 位作者 耿亚洪 卢大炎 张成武 曾明涛 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2012年第4期627-637,共11页
The influence of alkali on astaxanthin and the optimal working wave length for measurement of astaxanthin from Haematocoecus crude extract were investigated, and a spectrophotometric method for precise quantification ... The influence of alkali on astaxanthin and the optimal working wave length for measurement of astaxanthin from Haematocoecus crude extract were investigated, and a spectrophotometric method for precise quantification of the astaxanthin based on the method of Boussiba et al. was established. According to Boussiba's method, alkali treatment destroys chlorophyll. However, we found that: 1) carotenoid content declined for about 25% in Haematococcus fresh cysts and up to 30% in dry powder of Haematococcus broken cysts after alkali treatment; and 2) dimethyl sulfoxide (DMSO)-extracted chlorophyll of green Haematococeus bares little absorption at 520-550 nm. Interestingly, a good linear relationship existed between absorbance at 530 nm and astaxanthin content, while an unknown interference at 540-550 nm was detected in our study. Therefore, with 530 nm as working wavelength, the alkali treatment to destroy chlorophyll was not necessary and the influence of chlorophyll, other carotenoids, and the unknown interference could be avoided. The astaxanthin contents of two samples were measured at 492 nm and 530 nm; the measured values at 530 nm were 2.617 g/100 g and 1.811 g/100 g. When compared with the measured values at 492 nm, the measured values at 530 nm decreased by 6.93% and 11.96%, respectively. The measured values at 530 nm are closer to the true astaxanthin contents in the samples. The data show that 530 nm is the most suitable wave length for spectrophotometric determination to the astaxanthin in Haematococcus crude extract. 展开更多
关键词 Haematococcus pluvialis astaxanthin quantification SPECTROPHOTOMETRY
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