This study was carried out as a result of floristic expeditions in flora of Agri Mountain in Igdir between 2011 and 2014. As it is known, having diverse climatic types, Turkey has different and rich flora and vegetati...This study was carried out as a result of floristic expeditions in flora of Agri Mountain in Igdir between 2011 and 2014. As it is known, having diverse climatic types, Turkey has different and rich flora and vegetation. Over 380 plant species have been found in Agn Mountain up to now. From these species, 36 of them belong to Fabaceae family and they grow 900-3,000 m elevation above sea level on Agri Mountain. Fabaceae species are known throughout the world. As a result of researches carried out on Agri Mountain, 36 species were found, 10 of which belong to Fabaceae family. After Devis, an outstanding flora research has not been carried out in Agri Mountain so far. As a result of the researches carried out between 2011 and 2014, the number of genera reached to 10 and the number of species to 36. It was found out 17 species for the genus of Astragalus, two for Coronilla, five for Trifolium, three for Lathyrus, three for Onobrychis, three for Vicia and one for each Glycyrrhiza, Melilotus and Sophora. Eleven of these species are endemic. Those endemic species were： Astragalus aduncus Willd., Astragalus antalyensis, Astragalus davisii Chamb and Matthews., Astragalus eriocephalus Willd. subsp, eriocephalus Willd., Astragalus chaldiranicus Kit Tan and Sorger, Astragalus pinetorum Boiss., Astragalus fumosus, Astragalus vesicaris L. subsp, agridagensis Y. Zeynalov, Trifolium davissi Hossain, Lathyrus carcinus P. H. Davis, Onobrychis araxina Schischkin.展开更多
Background： Ketoconazole （KET）, an antifungal drug, has adverse effects on the male reproductive system. Pre-treatments with antioxidant plant against testicular damage induced by KET are required. The flowers of C...Background： Ketoconazole （KET）, an antifungal drug, has adverse effects on the male reproductive system. Pre-treatments with antioxidant plant against testicular damage induced by KET are required. The flowers of Clitoria tematea （CT） are proven to have hepatoprotective potential. However, the protective effect on KET-induced testicular damage has not been reported. Objective： To investigate the protective effect of CT flower extracts with antioxidant activity on male reproductive parameters including sperm concentration, serum testosterone level, histopathology of the testis, and testicular tyrosine phosphorylation levels in rats induced with KET. Methods： The antioxidant activity of CT flower extracts was determined using 2,2-diphenyl-1-picrylhydrazyl （DPPH） and ferric reducing antioxidant power （FRAP） assays. Male rats were treated with CT flower extracts （10, 50, or 100 mg/kg BW） or distilled water via a gastric tube for 28 d （preventive period： Days 1-21） and induced by KET （100 mg/kg BW） via intraperitoneal injection for 7 d （induction period： Days 22-28）. After the experiment, all animals were examined for the weights of the testis, epididymis plus vas deferens and seminal vesicle, serum testosterone levels, sperm concentration, histological structures and diameter of testis, and testicular tyrosine phosphorylation levels by immunoblotting. Results： The CT flower extracts had capabilities for DPPH scavenging and high reducing power. At 100 mg/kg BW, the extract had no toxic effects on the male reproductive system. Significantly, in CT＋KET groups, CT flower extracts （50 and 100 mg/kg BW） alleviated the reduction of reproductive organ weight parameters, testosterone levels, and sperm concentration. In addition, CT flower extracts gave protection from testicular damage in KET-induced rats. Moreover, in the CT100＋KET group, CT flower extracts significantly enhanced the expression of a testicular 50-kDa tyrosine phosphorylated protein compared with that of other groups. Conclusions： C. ternatea flower extracts possessing antioxidant activity are not harmful to the male reproductive system and can protect against testicular damage in KET-induced rats.展开更多
文摘This study was carried out as a result of floristic expeditions in flora of Agri Mountain in Igdir between 2011 and 2014. As it is known, having diverse climatic types, Turkey has different and rich flora and vegetation. Over 380 plant species have been found in Agn Mountain up to now. From these species, 36 of them belong to Fabaceae family and they grow 900-3,000 m elevation above sea level on Agri Mountain. Fabaceae species are known throughout the world. As a result of researches carried out on Agri Mountain, 36 species were found, 10 of which belong to Fabaceae family. After Devis, an outstanding flora research has not been carried out in Agri Mountain so far. As a result of the researches carried out between 2011 and 2014, the number of genera reached to 10 and the number of species to 36. It was found out 17 species for the genus of Astragalus, two for Coronilla, five for Trifolium, three for Lathyrus, three for Onobrychis, three for Vicia and one for each Glycyrrhiza, Melilotus and Sophora. Eleven of these species are endemic. Those endemic species were： Astragalus aduncus Willd., Astragalus antalyensis, Astragalus davisii Chamb and Matthews., Astragalus eriocephalus Willd. subsp, eriocephalus Willd., Astragalus chaldiranicus Kit Tan and Sorger, Astragalus pinetorum Boiss., Astragalus fumosus, Astragalus vesicaris L. subsp, agridagensis Y. Zeynalov, Trifolium davissi Hossain, Lathyrus carcinus P. H. Davis, Onobrychis araxina Schischkin.
基金Project supported by the Faculty of Medicine,Khon Kaen University(No.I 55222),Thailand
文摘Background： Ketoconazole （KET）, an antifungal drug, has adverse effects on the male reproductive system. Pre-treatments with antioxidant plant against testicular damage induced by KET are required. The flowers of Clitoria tematea （CT） are proven to have hepatoprotective potential. However, the protective effect on KET-induced testicular damage has not been reported. Objective： To investigate the protective effect of CT flower extracts with antioxidant activity on male reproductive parameters including sperm concentration, serum testosterone level, histopathology of the testis, and testicular tyrosine phosphorylation levels in rats induced with KET. Methods： The antioxidant activity of CT flower extracts was determined using 2,2-diphenyl-1-picrylhydrazyl （DPPH） and ferric reducing antioxidant power （FRAP） assays. Male rats were treated with CT flower extracts （10, 50, or 100 mg/kg BW） or distilled water via a gastric tube for 28 d （preventive period： Days 1-21） and induced by KET （100 mg/kg BW） via intraperitoneal injection for 7 d （induction period： Days 22-28）. After the experiment, all animals were examined for the weights of the testis, epididymis plus vas deferens and seminal vesicle, serum testosterone levels, sperm concentration, histological structures and diameter of testis, and testicular tyrosine phosphorylation levels by immunoblotting. Results： The CT flower extracts had capabilities for DPPH scavenging and high reducing power. At 100 mg/kg BW, the extract had no toxic effects on the male reproductive system. Significantly, in CT＋KET groups, CT flower extracts （50 and 100 mg/kg BW） alleviated the reduction of reproductive organ weight parameters, testosterone levels, and sperm concentration. In addition, CT flower extracts gave protection from testicular damage in KET-induced rats. Moreover, in the CT100＋KET group, CT flower extracts significantly enhanced the expression of a testicular 50-kDa tyrosine phosphorylated protein compared with that of other groups. Conclusions： C. ternatea flower extracts possessing antioxidant activity are not harmful to the male reproductive system and can protect against testicular damage in KET-induced rats.
