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血吸附法浓缩NDV病毒及与其他几种方法的比较 被引量:5
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作者 李燕 王恩秀 陈溥言 《生物技术》 CAS CSCD 2002年第2期30-31,共2页
用人O型红细胞吸附—释放病毒的方法纯化被感染的鸡胚尿囊液中的新城疫病毒 (NDV) ,然后分别对纯化的病毒液及弃去的上清进行血凝检测。结果表明 ,使用 1%的红细胞悬液只能吸附部分病毒 ,损失较大 ,只有将红细胞悬液的浓度增加到2 0 %... 用人O型红细胞吸附—释放病毒的方法纯化被感染的鸡胚尿囊液中的新城疫病毒 (NDV) ,然后分别对纯化的病毒液及弃去的上清进行血凝检测。结果表明 ,使用 1%的红细胞悬液只能吸附部分病毒 ,损失较大 ,只有将红细胞悬液的浓度增加到2 0 %时 ,才基本将病毒全部吸附住。同时还比较了差速离心、PEG沉淀、PEG包埋等方法。 展开更多
关键词 血吸附法 浓缩 NDV病毒 纯化 新城疫病毒
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滴定痘苗病毒的噬斑血吸附法的优化 被引量:4
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作者 董小曼 田博 +4 位作者 董翊 赵素华 陈秀珍 范淑兰 吕洋 《中国生物制品学杂志》 CAS CSCD 2007年第11期855-857,共3页
目的对滴定痘苗病毒的噬斑血吸附法进行优化,克服该方法稳定性差、结果数据偏差大的问题。方法通过预试验确定痘苗病毒的最佳稀释倍数、病毒吸附温度及鸡血球加入方法等参数。采用优化的噬斑血吸附法,对天坛株痘苗病毒的原始种子批、主... 目的对滴定痘苗病毒的噬斑血吸附法进行优化,克服该方法稳定性差、结果数据偏差大的问题。方法通过预试验确定痘苗病毒的最佳稀释倍数、病毒吸附温度及鸡血球加入方法等参数。采用优化的噬斑血吸附法,对天坛株痘苗病毒的原始种子批、主种子批、工作种子批和参考苗进行滴定,计算变异系数(CV),确定试验内、试验间和不同实验人员间的精密度。利用Qstat软件分析本次与以往滴定结果之间的关系。结果病毒的最佳稀释度为10-6,最佳吸附温度为25~30℃,加入鸡血球的最佳方法为病毒吸附72h后,弃去部分培养液,再加入鸡血球。4种样品测定结果的试验内、试验间和不同实验人员间的变异系数均小于5%,病毒滴度分别为(7.12±0.18)LgPFU/ml、(7.79±0.21)LgPFU/ml、(7.88±0.17)LgPFU/ml和(7.64±0.14)LgPFU/ml,均高于以往滴定结果。本次与以往滴定结果之间存在线性相关性。结论优化了滴定痘苗病毒滴度的噬斑血吸附法,确定了天坛株痘苗病毒的原始种子批、主种子批、工作种子批和参考苗的滴度,为天花疫苗多项检定试验提供了可靠依据。 展开更多
关键词 痘苗病毒 噬斑血吸附法 病毒滴定 优化
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反向间接固相免疫血吸附法检测外环境物体表面HB_sAg
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作者 郭开均 刘雄 周贤会 《四川省卫生管理干部学院学报》 1995年第4期38-39,共2页
反向间接固相免疫血吸附法检测外环境物体表面HB_sAg郭开均,刘雄,周贤会成都中医药大学附院,成都陆军总医院,四川省卫干院附院外环境HBsAg的测定是了解环境被HBV污染的有效方法之一,对预防乙肝、防止乙肝在易感人群... 反向间接固相免疫血吸附法检测外环境物体表面HB_sAg郭开均,刘雄,周贤会成都中医药大学附院,成都陆军总医院,四川省卫干院附院外环境HBsAg的测定是了解环境被HBV污染的有效方法之一,对预防乙肝、防止乙肝在易感人群中的传播具有重要意义,公共场所HB?.. 展开更多
关键词 反向间接固相免疫血吸附法 检测 外环境 HBSAG
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血仿膜吸附法无肝素透析治疗蝰蛇咬伤致急性肾功能衰竭3例体会 被引量:1
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作者 王建红 吕恒华 +3 位作者 龚平 杜娟 陈彦君 谢方 《透析与人工器官》 2000年第3期37-38,共2页
关键词 仿膜吸附 肝素 透析疗 蝰蛇咬伤 急性肾功能衰竭 功能
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血仿膜吸附法无肝素透析技术在高危出血尿毒症中的应用 被引量:1
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作者 吴小宁 《华夏医学》 CAS 2006年第5期1047-1049,共3页
关键词 仿膜吸附 无肝素透析技术 尿毒症
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血仿膜肝素吸附法联合小剂量低分子量肝素在高危出血患者血液透析中的应用
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作者 吴恩亮 王清华 高慧芳 《滨州医学院学报》 2002年第5期378-379,共2页
关键词 仿膜肝素吸附 低分子量肝素 高危出患者 液透析 肾功能衰竭
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不同肝素溶媒在血仿膜吸附法无肝素透析中应用的临床观察 被引量:2
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作者 张艺央 耿爱枝 《临床军医杂志》 CAS 2005年第6期691-692,共2页
目的探讨不同肝素溶媒行血仿膜吸附法无肝素透析的效果及可行性。方法对29例合并出血或有出血倾向的维持性血透患者,采用同一患者自身前后交叉对照的方法观察生理盐水(NS)和5%葡萄糖(GS)为肝素溶媒的透析效果。结果29例无一例出血加重,N... 目的探讨不同肝素溶媒行血仿膜吸附法无肝素透析的效果及可行性。方法对29例合并出血或有出血倾向的维持性血透患者,采用同一患者自身前后交叉对照的方法观察生理盐水(NS)和5%葡萄糖(GS)为肝素溶媒的透析效果。结果29例无一例出血加重,NS与5%GS两种肝素溶媒其透析器的BUN和Scr清除率无明显差别(P>0.05),但5%GS为肝素溶媒时透析器凝血情况明显低于NS(P<0.05)。结论在血仿膜吸附法无肝素透析中5%GS作为肝素溶媒的吸附效果优于NS。 展开更多
关键词 5%葡萄糖 生理盐水 仿膜吸附 无肝素 液透析 肝素溶媒
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血仿膜肝素吸附法在高危出血液透析析患者中的应用 被引量:8
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作者 石春珍 徐青 《中国血液净化》 2006年第10期748-749,共2页
血液透析是一个体外循环过程,血液与管路、透析膜的接触,可触发机体的凝血系统,治疗中必须使用抗凝剂以防止血栓的形成。临床上经常遇到各种原因导致的急、慢性肾功能损害并出血患者,普通肝素抗凝治疗显然会加重患者的出血情况,无... 血液透析是一个体外循环过程,血液与管路、透析膜的接触,可触发机体的凝血系统,治疗中必须使用抗凝剂以防止血栓的形成。临床上经常遇到各种原因导致的急、慢性肾功能损害并出血患者,普通肝素抗凝治疗显然会加重患者的出血情况,无肝素透析是基层医院(不包括使用无肝素透析器)应用得最多的一种方法,但其操作步骤多,护理工作量大,治疗中频繁的生理盐水冲洗对患者血流动力学影响大近几年来低分子肝素以其抗凝效果确切、对血小板功能影响小而广泛用于临床,但仍不能完全避免对系统凝血功能的影响。江西省九江市中医院血液净化中心自2002年以来运用血仿膜肝素吸附法(HFHD)治疗高危出血的透析患者,现将其治疗方法及疗效作一次回顾性小结,结果如下。 展开更多
关键词 仿膜肝素吸附 液透析 高危出 无肝素透析 抗凝治疗 流动力学影 生理盐水冲洗 液净化中心
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应用血仿膜肝素吸附法低钠透析抢救特大面积烧伤后高钠血症
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作者 王淑芬 杜娟 +2 位作者 吕恒华 钦军 龚平 《透析与人工器官》 1998年第3期25-25,共1页
关键词 仿膜肝素吸附 特大面积烧伤 高钠 液透析 烧伤后 小板计数 透析液 透析治疗 低钠 烧伤患者
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急慢性肾功能衰竭伴出血患者两种无肝素透析方法的疗效比较 被引量:2
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作者 孟艳 陈江华 《浙江医学》 CAS 2008年第3期238-239,共2页
血液透析是一个体外循环过程,血液与透析管路及透析膜的接触,易触发并激活机体的凝血系统,导致凝血的发生,因此治疗过程中必须使用抗凝剂预防血栓的形成。肝素作为一种抗凝效果较好的抗凝剂已广泛应用于透析治疗中,但在对各种原因... 血液透析是一个体外循环过程,血液与透析管路及透析膜的接触,易触发并激活机体的凝血系统,导致凝血的发生,因此治疗过程中必须使用抗凝剂预防血栓的形成。肝素作为一种抗凝效果较好的抗凝剂已广泛应用于透析治疗中,但在对各种原因导致的急慢性肾功能衰竭合并出血患者行透析治疗时,普通肝素抗凝治疗往往会加重出血,为此临床上尝试了许多减少出血的透析方法,如无肝素透析、低分子肝素透析、体外肝素化透折以及血仿膜吸附法无肝素透析等。我院对急慢性肾功能衰竭合并出血患者采用血仿膜吸附法无肝素透析和无肝素透析两种方法进行治疗,旨在比较两种方法的疗效,现将结果报道如下。 展开更多
关键词 急慢性肾功能衰竭 无肝素透析 患者 透析方 疗效比较 仿膜吸附 透析治疗 液透析
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局部枸橼酸抗凝血液透析与改良无肝素透析治疗尿毒症高危出血的临床观察 被引量:7
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作者 吴小宁 刘焕皓 《临床肾脏病杂志》 2009年第7期319-321,共3页
目前,为了选择一种更安全有效的血液透析方法治疗高危出血或活动性出血的尿毒症患者,我们对局部枸橼酸抗凝(RCA)血液透析和血仿膜肝素吸附法无肝素透析(HCHD)进行了临床应用并将2种方法进行了对比分析,现报道如下。
关键词 抗凝液透析 尿毒症患者 无肝素透析 枸橼酸抗凝 高危出 透析治疗 临床观察 仿膜肝素吸附
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Incidence of toxoplasmosis in patients with cirrhosis 被引量:6
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作者 Sebnem Ustun Umit Aksoy +1 位作者 Hande Dagci Galip Ersoz 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第3期452-454,共3页
AIM:It is known that toxoplasmosis rarely leads to various liver pathologies,most common of which is granulomatose hepatitis in patients having normal immune systems.Patients who have cirrhosis of the liver are subjec... AIM:It is known that toxoplasmosis rarely leads to various liver pathologies,most common of which is granulomatose hepatitis in patients having normal immune systems.Patients who have cirrhosis of the liver are subject to a variety of cellular as well as humoral immunity disorders.Therefore,it may be considered that toxoplasmosis can cause more frequent and more severe diseases in patients with cirrhosis and is capable of changing the course of the disease.The aim of this study was to investigate the frequency of toxoplasmosis in patients with cirrhosis. METHODS:Serum samples were taken from 108 patients with cirrhosis under observation in the Hepatology Polyclinic of the Gastroenterology Clinic,and a control group made up of 50 healthy blood donors.IFAT and ELISA methods were used to investigate the IgG and IgM antibodies,which had developed from these sera. RESULTS:Toxoplasma IgG and IgN antibody positivity was found in 74 (68.5%) of the 108 cirrhotic patients and 24 (48%) of the 50 people in the control group.The difference between them was significant (P<0.05). CONCLUSION:In conclusion,it was found that the toxopiasma sero-prevalence in the cirrhotic patients in this study was higher.Cirrhotic patients are likely to form a toxoplasma risk group.More detailed studies are needed on this subject. 展开更多
关键词 Adult Animals Antibodies Protozoan Female Humans INCIDENCE Liver Cirrhosis Male Middle Aged TOXOPLASMA TOXOPLASMOSIS
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Study on Removal of Bilirubin with Magnetic Affinity Separation Technique 被引量:2
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作者 徐辉 张国亮 +1 位作者 张凤宝 王淑兰 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2003年第4期456-459,共4页
An affinity adsorbent, Cibacron Blue 3GA immobilized magnetic polyvinyl alcohol (PVA) microspheres was used for bilirubin removal taking the advantage of easy separation of magnetic sorbent from the biosystem. Fe3O4 s... An affinity adsorbent, Cibacron Blue 3GA immobilized magnetic polyvinyl alcohol (PVA) microspheres was used for bilirubin removal taking the advantage of easy separation of magnetic sorbent from the biosystem. Fe3O4 superparamagnetic particles was synthesized with hydrothermal reaction of ferrous chloride (FeCl2) and ferric chloride (FeCl3). Such magnetic particles are then encapsulated in biocompatible PVA to form magnetic polymer microspheres sized from 2 to 15 nm with hydroxyl groups on its surface. Cibacron Blue 3GA, a dye-ligand, was covalently coupled with the polyvinyl alcohol through the nucleophilic reaction between the chloride of its triazine ring and the hydroxyl groups of PVA molecules under alkaline condition. The affinity adsorbent carried 21.1μmol Cibacron Blue 3GA per gram magnetic polymer microspheres was used to remove unconjugated and conjugated bilirubin from the solution which was composed of bilirubin or bilirubin and protein. After the adsorption, the adsorbent loaded with bilirubin was removed easily in the magnetic field. 展开更多
关键词 Cibacron Blue 3GA BILIRUBIN magnetic affinity separation
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Interleukin-2 and Interleukin-6 in Recurrent Genital Herpes Patients
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作者 张敏 张谊之 《Chinese Journal of Sexually Transmitted Infections》 2002年第3期46-47,共2页
Objective:To study the cellular immunity status of patientswith recurrent genital herpes. Methods: Serum levels of interlukin-2 and its solublereceptor and interlukin-6 were measured by ELISA in 34patients with recurr... Objective:To study the cellular immunity status of patientswith recurrent genital herpes. Methods: Serum levels of interlukin-2 and its solublereceptor and interlukin-6 were measured by ELISA in 34patients with recurrent genital herpes. Results: Serum levels of IL-2 and IL-6 were significantlydecreased in patients compared to healthy controls (P<0.01),and the level of sIL-2R was significantly increased in patientswith recurrent genital herpes (P<0.01). There were nosignificant differences in all variables amongst patientsregarding relapse stage and remission stage (P>0.05). Conclusion: There was a cellular immune deficiency inpatients with recurrent genital herpes. 展开更多
关键词 Genital Herpes IL-2 SIL-2R IL-6
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Immune Function Changes among HIV+/ARC/AIDS Patientsand Its Relationship to Disease Progression
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作者 冯铁建 何建凡 +1 位作者 李良成 陈琳 《Chinese Journal of Sexually Transmitted Infections》 2002年第2期37-41,共5页
Objective: To analyze HIV replication and immune functionchanges among Chinese patients with HIV/ARC/AIDS andtheir association with disease progression. Methods: T cell subsets, plasma cytokine concentrations andviral... Objective: To analyze HIV replication and immune functionchanges among Chinese patients with HIV/ARC/AIDS andtheir association with disease progression. Methods: T cell subsets, plasma cytokine concentrations andviral loads from 42 HIV+ individuals, and 13 ARC/AIDSpatients, and 10 controls were analyzed by flow cytometry(FCM), quantitative ELISA and reversetranscription-polymerase chain reaction (RT-PCR),respectively. Results: CD4 cell counts and plasma IL-2 in HIV/AIDSpatients were significantly less than in normal control subjects(P<0.001). The plasma concentrations sIL-2R, TNF-α, andNeopterin increased significantly with decreasing CD4 cellcounts. Plasma IL-2 among AIDS/ARC patients was also lessthan in HIV+ patients (P<0.01). CD4 cell counts, the ratio ofCD4 to CD8 and plasma IL-2 levels decreased significantlywith infection duration. CD4 cell counts declined an averageof 43/ml per year. In contrast, the concentration of plasmasIL-2R, sTNFR-I, and Neopterin increased an average of9.03pg/ml, 8.69pg/ml, 2.11ng/ml per yean respectively.Furthermore, a significant reverse linear correlation wasobserved between CD4 cell count, CD4/CD8 ratio, and CD3,CD4 and CD8 counts with plasma levels of sTNFR-I,Neopterin, and HIV RNA load. A positive linear correlationwas noted between plasma sIL-2R levels and changes ofplasma IL-6 level (P<0.001), IL-10(P<0.001), TNF-α(P<0.001),sTNFR-I (P<0.005), and Neopterin (P<0.002) and betweenIL-6 and TNF-α(P<0.001), Neopterin and IL-10 (P<0.05),sTNFR-I (P<0.001), plasma viral load and sTNFR-I (P<0.001),and Neopterin (P<0.002). Conclusion: These findingr suggest a close relationshipbetween IL-6 and TNF-aimmune activation, HIV replicationand disease progression in primary HIV infections and AIDSpatients. Declining CD4 cells and plasma IL-2, and increasingviral load, sIL-2R, TNF-α, sTNFR-I, and Neopterin might beconsidered as important predictors of the progression of HIVinfection to AIDS. 展开更多
关键词 HIV infection Viral load CYTOKINE T cell subset
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外周血干扰素-γ释放试验诊断结核性胸膜炎的假阴性影响因素分析 被引量:8
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作者 陈彬 朱涛 李长毅 《重庆医学》 CAS 2020年第4期534-538,543,共6页
目的探讨外周血干扰素-γ释放试验(IGRAs)诊断结核性胸膜炎假阴性的独立影响因素。方法采用回顾性分析的方法,纳入2014年1月至2019年5月在重庆医科大学附属第二医院呼吸内科住院治疗,并根据细菌学和(或)组织学确诊为结核性胸膜炎的患者... 目的探讨外周血干扰素-γ释放试验(IGRAs)诊断结核性胸膜炎假阴性的独立影响因素。方法采用回顾性分析的方法,纳入2014年1月至2019年5月在重庆医科大学附属第二医院呼吸内科住院治疗,并根据细菌学和(或)组织学确诊为结核性胸膜炎的患者144例,采集患者人口学及临床资料,根据全血酶联测定免疫吸附测定法(QFT-GIT)进行IGRAs的结果将患者分为假阴性组和阳性组,应用Logistic回归分析QFT-GIT在结核性胸膜炎诊断中的假阴性的影响因素。结果结核菌素试验(PPD)阴性结果[Exp(B)=6.451,95%CI(2.951~14.105),P=0.000]、外周血嗜酸性粒细胞(EOS)计数[Exp(B)=0.057,95%CI(0.004~0.811),P=0.034]是QFT-GIT假阴性的独立影响因素。结论对于EOS较高和PPD阴性的胸腔积液患者,若出现QFT-GIT阴性的结果,需结合临床进行综合判断,不能完全排除结核性胸膜炎可能。 展开更多
关键词 结核 胸膜 酶联测定免疫吸附测定 假阴性 危险因素
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Plasma thrombopoietin levels in patients with aplastic anemia and idiopathic thrombocytopenic purpura 被引量:4
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作者 顾静 陆璐 +1 位作者 徐瑞容 陈秀芳 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第7期983-986,143,共4页
OBJECTIVE: To evaluate the role of thrombopoietin (TPO) in the pathology of chronic thrombocytopenic disease. METHODS: We measured the endogenous plasma concentration of TPO in 40 patients with acquired aplastic anaem... OBJECTIVE: To evaluate the role of thrombopoietin (TPO) in the pathology of chronic thrombocytopenic disease. METHODS: We measured the endogenous plasma concentration of TPO in 40 patients with acquired aplastic anaemia (AA) and in 32 patients with idiopathic thrombocytopenic purpura (ITP) by a sensitive Sandwich enzyme-linked immunosorbent assay (ELISA) and compared the results. RESULTS: Plasma TPO concentrations were significantly higher in AA patients (774 +/- 393 pg/ ml) in comparison with healthy control subjects (55 +/- 34 pg/ml, P 0.05). There was also no relationship between their plasma TPO levels and platelet counts. CONCLUSIONS: TPO levels may be regulated not only by platelets but also by megakaryocytes in AA and ITP, and measurement of TPO levels is useful for diagnosing thrombocytopenia and understanding the pathophysiology of thrombocytopenia. 展开更多
关键词 Adolescent Adult Anemia Aplastic Female Humans Male Middle Aged Purpura Thrombocytopenic Idiopathic RNA Messenger THROMBOPOIETIN
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Effect of scraping therapy on Interleukin-1 in serum of rats with lumbar disc herniation 被引量:23
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作者 Rongrong Jiang Guihua Xu +3 位作者 Hua Chen Jie Li Tao Yang Yang Guo 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2013年第1期109-113,共5页
OBJECTIVE:To explore the effect of scraping therapy on the Interleukin-1 (IL-1) levels of rats with lumbar disc herniation (LDH). METHODS: Fifty male rats were devided into a blank group (A), a sham operation group (B... OBJECTIVE:To explore the effect of scraping therapy on the Interleukin-1 (IL-1) levels of rats with lumbar disc herniation (LDH). METHODS: Fifty male rats were devided into a blank group (A), a sham operation group (B), a model group (C), a scraping group (D), and a drug group (E). The rats in the group B were treated with sham operation, and groups C, D and E were made into the LDH model by operation. After operation group C were treated with no interventions, D were given scraping and E were fed with azathioprine Then the IL-1 levels of different groups were detected by enzyme-linked immuno sorbent assay method. And the transplanted coccygeal vertebra discs were observed by pathological section. RESULTS: The IL-1 levels in the groups C, D, and E were significantly higher than those in the groups A and B (all P<0.01), which proved the operationwas successful.The IL-1 levels in the groups D and E at different periods had statistical significance (F= 414.158, P<0.01). The treatment periods and interventions have interation (F=46.613, P<0.01). Multiple comparison results showed that the IL-1 levels in the groups D and E was significantly lower than that in the group C (P<0.01), while the IL-1 levels between the groups D and E had no statistical significance (P>0.05). Moreover, pathological section indicated that immuno-inflammatory response was hardly found in coccygeal vertebra discs in the groups A and B, while local immuno-inflammatory responses of the groups D and E were much lighter than that of the group C. CONCLUSION: Scraping therapy could inhibit the immuno-inflammatory responses in the rats with LDH caused by transplantation of autologous nu cleus pulposus. 展开更多
关键词 Scraping therapy Intervertebral diskdisplacement INTERLEUKIN-1
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