Objectives: To develop a multi-nested polymerase chain reaction in an assay to detect early Treponema pallidum and Haemophilius ducreyi DNA in the swabs of genital ulcers. Methods: Four pairs of outer and inner primer...Objectives: To develop a multi-nested polymerase chain reaction in an assay to detect early Treponema pallidum and Haemophilius ducreyi DNA in the swabs of genital ulcers. Methods: Four pairs of outer and inner primers, specific to the basic membrane protein gene of Treponema pallidum and to the 16s rRNA gene of H ducreyi were synthesized. The multi-nested PCR was developed and applied to detect Treponema pallidum and Haemophilus dicreyi in clinical swabs. Result: The two samples of standard strains of Haemophilus ducreyi and one Treponema pallidum were amplified and showed 309-bp rRNA gene of Haemophilus ducreyi and 506-bp DNA of Treponema palidum, respectively. Out of 51 samples of genital ulcer detected, 29 showed Treponema pallidum positive product and no Haemophilus ducreyi DNA was found. Conclusion: The multi-nested PCR for Treponema pallidum and Haemophilus ducreyi could be useful for early detection and distinguishing diagnosis between syphilis and chancroid.展开更多
Salmonella can invade non-phagocytic cells through its type Ⅲ secretion system （T3SS-1）, which induces a Trigger entry process. This study showed that Salmonella enterica, subspecies enterica serovar Enteritidis ca...Salmonella can invade non-phagocytic cells through its type Ⅲ secretion system （T3SS-1）, which induces a Trigger entry process. This study showed that Salmonella enterica, subspecies enterica serovar Enteritidis can also invade cells via the Rck outer membrane protein. Rck was necessary and sufficient to enable non-invasive E. coli and Rckcoated beads to adhere to and invade different cells. Internalization analysis of latex beads coated with different Rck peptides showed that the peptide containing amino acids 140-150 promoted adhesion, whereas amino acids between 150 and 159 modulated invasion. Expression of dominant-negative derivatives and use of specific inhibitors demonstrated the crucial role of small GTPases Racl and Cdc42 in activating the Arp2/3 complex to trigger formation of actin-rich accumulation, leading to Rck-dependent internalization. Finally, scanning and transmission electron microscopy with Rck-coated beads and E. coli expressing Rck revealed microvillus-like extensions that formed a Zipper-like structure, engulfing the adherent beads and bacteria. Overall, our results provide new insights into the Salmonella T3SS-independent invasion mechanisms and strongly suggest that Rck induces a Zipper-like entry mechanism. Consequently, Salmonella seems to be the first bacterium found to be able to induce both Zipper and Trigger mechanisms to invade host cells.展开更多
Sea cucumber and cordyceps sinensis are used as both food and traditional medicines in Asia. This study was carried out in order to investigate the hpyerglycemic effect of a mixture of sea cucumber （Apostichopusjapon...Sea cucumber and cordyceps sinensis are used as both food and traditional medicines in Asia. This study was carried out in order to investigate the hpyerglycemic effect of a mixture of sea cucumber （Apostichopusjaponicas） and cordyceps sinensis （Cor-dyceps militaris） （SCC） in diabetic rat and explore the mechanism underlining such an effect. The diabetic model rat was induced with intraperitoneal injection of streptozotocin （STZ）. The diabetic model rats were randomly divided into control group （0.9% NaC1）, low dose group （300 mg SCC.（kg body weight）-1） and high dose group （1200 mg SCC （kg body weight）-l）. Sodium chloride and SCC were intragastrically administered once a day for 35 d. Changes in fasting serum glucose and serum insulin content, oral glucose tolerance and liver and muscle glycogen content were routinely evaluated. Pancreas tissue and β-cells of islets were observed under both optical and transmission electronic microscope, respectively. The abundance of glucose metabolism-relating genes in gastrocnemius and epididymal adipose tissue was determined with either reverse transcription PCR （RT-PCR） or western blotting. Results showed that SCC significantly decreased fasting serum glucose content, improved glucose tolerance and increased serum insulin and glycogen content; repaired STZ-injured β-cells of diabetic rat, and increased the expression of phosphatidylinositol 3 kinase （PI（3）K）, protein kinase B （PKB） and glucose transporter 4 （Glut4） encoding protein in both gastroenemius and adipose tissue, and Glut4 encoding gene in peripheral tissue. Our findings demonstrated that SCC exerted an anti-hyperglycemic effect by repairing β-cells and promoting insulin-mediated signal transduction pathway in insulin-sensitive gastrocnemius and adipose tissue.展开更多
Microbiological investigation of the fossil animals preserved in permafrost represents obvious interest for science. Lack of data in this sphere gives even greater importance to any findings giving us opportunity to l...Microbiological investigation of the fossil animals preserved in permafrost represents obvious interest for science. Lack of data in this sphere gives even greater importance to any findings giving us opportunity to learn more about remote past of microorganisms. In this respect, preserved remains of fossil are considered as unique biological materials for scientific investigations. Bacillus bacteria strains isolated from the paleomicrobiota of mammoth fauna are not only have high durability （20-30 thousand of years） in permafrost, but are still able to produce biologically active substances. Strains of bacteria of the genus Bacillus, isolated from the tissues of the representatives of the mammoth fauna have strong antagonistic properties to hemolytic streptococci--Streptococcus equi, pathogenic for animals--Salmonella abortus equi, also toxigenic micromycetes genera Aspergillus, Alternuria, Penicillum and fungal pathogens of plant diseases--Botrytis cimeria and Fuzarium oxysporium. The strains of bacteria of the genus Bacillus are not pathogenic to plants and animals, but initially resistant to wide range of antibiotics. Dominance strains of Bacillus bacteria, producing strong bacteriocins in the soft tissues of fossil animals, contributing to their long cryo bio conservation. In addition, bacterial strains of Bacillus subtilis, isolated from paleo microbiota have strong oxidizing properties. Microbiota of fossils preserved in permafrost of Yakutia is of particular interest for microbiology and modem biotechnology.展开更多
Based on rpoB gene micro array as target gene, we are going to use gene chip technology to test 24 mycobacterium standard specimens, 8 non-mycobacterium specimens and 86 mycobacterium clinical isolated specimens. As a...Based on rpoB gene micro array as target gene, we are going to use gene chip technology to test 24 mycobacterium standard specimens, 8 non-mycobacterium specimens and 86 mycobacterium clinical isolated specimens. As a result, after mycobacterium and non-mycobacterium standard specimens were duplicated by PCR, mycobacterium standard specimens reproduced 360bp DNA fragments; on the other hand, non-mycobacterium specimens did not reproduce any fragments except for hemolytic streptococcus and corynebacterium pseudodiphtheriticum which had the same results as mycobacterium standard specimens. Sensitive test is able to detect lpg tuberculosis mycobacterium DNA. The probe test showed that, among 21 oligonucleotide probes, probe-M. fortuitum and M. marinum were cross-hybrid; the other probes were specific. We used the new method to identify 126 mycobacterium clinical isolated specimens. The test results of this new method matched with conventional method. In conclusion, compared to the traditional method, the use of rpob gene chip technology to identify mycobacterium species will be faster, more accurate and higher value in application.展开更多
Objective： Diabetic nephropathy （DN） is one of the most common causes of end-stage renal failure. The pathogenesis of progressive renal injury is multifactorial and the mechanism by which hyperglycemia causes micro...Objective： Diabetic nephropathy （DN） is one of the most common causes of end-stage renal failure. The pathogenesis of progressive renal injury is multifactorial and the mechanism by which hyperglycemia causes microangiopathy is still poorly understood. The WNT pathway is activated in DN and regulating β-catenin protein levels is referred to as the canonical Wntβ-catenin pathway. Because the renin angiotensin system has been reported to be an important contributory factor in the pathophysiology of DN, exogenous administration of angiotensin Ⅱ receptor antagonist may be beneficial in counteracting some biochemical or functional changes of DN. The aim of the study was to determine the β-catenin expression and the possible protective effects of irbesartan, an angiotensin Ⅱ type 1 receptor blocker （ARB） in a rat model of streptozotocin（STZ）-induced diabetic nephropathy. Methods： STZ-induced DN in rats was assessed biochemically by measuring urine volume, protein and creatinine clearance as well as Kidney weight/body weight （KW/BW） and the index of mesangial expansion. Three groups of male Sprague-dawley rats were used. The first group consisted of non-diabetic control rats （control）. The second group was the untreated diabetic rats（STZ＋vehicle）. The third group consisted of diabeti rats treated with irbesartan, 50 mg/kg for 12 weeks （STZ＋irbesartan）. Immunohistochemical stainings and real time PCR for β-catenin were performed in renal cortex of rat modals. Results： Marked hyperglycemia, polyuria, proteinuria, renal hypertrophy, mesangial matrix expansion and glomerular hyperfiltration were observed in STZ diabetic rats. The levels of microalbuminuria and KW/BW in the STZ＋irbesartan group were lower than those in the STZ＋vehicle group （P〈0.05）. The up-regulated immunostaining and mRNA expression of β-catenin were decreased in renal cortic of the Irbesartan-treated diabetic group, but there was no significant difference compared to the untreated diabetic group. Conclusion： The data suggest that irbesartan ameliorates proteinuria and renal hypertrophy, charactered damages of STZ-induced early-stage DN in rats, but its effective drug target is not to inhibit the up-regulated expressions of β-catenin.展开更多
The aim of this study was to investigate the antihyperglycemic and antioxidant effects of Po （Portulaca oleracea） lyophilised aqueous extract in diabetic male Wistar rats. Diabetes was induced intraperitonially by a...The aim of this study was to investigate the antihyperglycemic and antioxidant effects of Po （Portulaca oleracea） lyophilised aqueous extract in diabetic male Wistar rats. Diabetes was induced intraperitonially by a single injection of STZ （streptozotocin） （60 mg/kg bw （body weight））. Twenty diabetic rats, weighing 263 ± 5 g, were divided into two groups fed a casein diet supplemented or not with Po extract （1 g/kg bw）, for four weeks. Control group （n = 6） received 0.23-0.25 mL of citrate buffer and was fed a standard diet during the experiment. The study was carried out at Oran University, Algeria and the entire experiments lasted from September 2011 to July 2012. Blood was obtained from the abdominal aorta of rats after fasting overnight and standard methods were used for the extraction of spices, determination of glycemia, insulinemia, lipid peroxidation and antioxidant enzymes activities. Portulaca oleracea treated compared to untreated rats, glycemia and HbAIc values were respectively 2.8- and 1.7-fold lower. TBARS （thiobarbituric acid reactive substances） concentrations were reduced in RBC （red blood cells） （-54%） and plasma （-65%）. Moreover, in liver and kidney, TBARS values were respectively 1.8- and 2-fold lower. SOD （superoxide dismutase） and GSH-Px （glutathione peroxidase） activities were increased respectively by ＋38% and ＋85%, in liver. GSSG-Red （glutathione reductase） activity was 1.9-fold higher in kidney, while CAT （catalase） was improved in kidney （＋48%）. In RBCs, SOD, GSH-Px, GSSH-Red and CAT activities were increased by 31%, 42%, 56% and ＋50%, respectively. These data have cast a new light on the actions of Portulaca oleracea and its antioxidant potential benefits in preventing diabetes and its complications.展开更多
Objective To set up a rapid and simple method for identificating bacteria by 16S 23SrDNA intergenic spacer regions (ISRs) Methods Polymorphic products of PCR from ISRs were selected on agarose gel and sequenced ...Objective To set up a rapid and simple method for identificating bacteria by 16S 23SrDNA intergenic spacer regions (ISRs) Methods Polymorphic products of PCR from ISRs were selected on agarose gel and sequenced directly using purified fragments by excising the gel without cloning Nucleotide sequences were compared with GenBank databases and analyzed by DNAMAN program Results There was only a single product in streptococcus genus after PCR amplification of 16S 23SrDNA ISRs Five streptococcal species were obtained from 7 strains of streptococcus Two major amplicons were consistently generated for 8 strains of Haemophilus influenzae (H influenzae) The sequence data showed that they all belonged to H influenzae type b on GenBank databases Conclusion PCR and direct sequencing of 16S 23SrDNA ISRs were very successful methods for bacterial species identification展开更多
OBJECTIVE: To investigate the effect of low frequency electro-pulse acupuncture(EA) on blood glucose in rats with streptozotocin-induced type 2diabetes, and the possible mechanism underlying the action.METHODS: Rat mo...OBJECTIVE: To investigate the effect of low frequency electro-pulse acupuncture(EA) on blood glucose in rats with streptozotocin-induced type 2diabetes, and the possible mechanism underlying the action.METHODS: Rat models were established with high fat feeding and intraperitoneal injection of streptozotocin(STZ)(30 mg/kg). Rats with a random blood glucose > 16.7 mmol/L and blood glucose at 2 h-point of oral glucose tolerance test(OGTT) > 11.1 mmol/L were included as diabetic rats, and randomly divided into model group, EA Weiwanxiashu(EX-B 3) group, EA Zusanli(ST 36)group, glimepiride group, and EA non-acupoint group(n = 12). EA(2 Hz continuous wave, 2 m A,20 min/day, 6 days/week, 4 weeks) and intra-gastric administration of glimepiride were applied as interventions. With fasting blood glucose and OGTT tested at the end of the intervention, thestudy observed the patterns of hypoglycemic effects. For mechanism study, it observes hematoxylin and eosin staining and Masson staining of pancreas paraffin sections, protein expression of glucagon-like peptide 1 receptor(GLP-1R) in the pancreas and skeletal muscle, glucose transporter 4(GLUT4) protein expression in skeletal muscle membrane, to detect whether EA controls blood glucose via regulation of GLP-1R.RESULTS: EA Weiwanxiashu(EX-B 3) significantly increased model rats' pancreas GLP-1R, and GLUT4 of skeletal muscle membrane; the therapy significantly decreased model rats' skeletal muscle GLP-1R, restored pancreas morphology, and reduced fasting blood glucose and insulin resistance indices.CONCLUSION: EA Weiwanxiashu(EX-B 3) alone has significant effect on glycemia. EA Weiwanxiashu(EX-B 3) plus glimepiride further strengthen the effect. The regulation of the GLP-1R in pancreas and skeletal muscle might be mechanism underpinning the effect.展开更多
文摘Objectives: To develop a multi-nested polymerase chain reaction in an assay to detect early Treponema pallidum and Haemophilius ducreyi DNA in the swabs of genital ulcers. Methods: Four pairs of outer and inner primers, specific to the basic membrane protein gene of Treponema pallidum and to the 16s rRNA gene of H ducreyi were synthesized. The multi-nested PCR was developed and applied to detect Treponema pallidum and Haemophilus dicreyi in clinical swabs. Result: The two samples of standard strains of Haemophilus ducreyi and one Treponema pallidum were amplified and showed 309-bp rRNA gene of Haemophilus ducreyi and 506-bp DNA of Treponema palidum, respectively. Out of 51 samples of genital ulcer detected, 29 showed Treponema pallidum positive product and no Haemophilus ducreyi DNA was found. Conclusion: The multi-nested PCR for Treponema pallidum and Haemophilus ducreyi could be useful for early detection and distinguishing diagnosis between syphilis and chancroid.
文摘Salmonella can invade non-phagocytic cells through its type Ⅲ secretion system （T3SS-1）, which induces a Trigger entry process. This study showed that Salmonella enterica, subspecies enterica serovar Enteritidis can also invade cells via the Rck outer membrane protein. Rck was necessary and sufficient to enable non-invasive E. coli and Rckcoated beads to adhere to and invade different cells. Internalization analysis of latex beads coated with different Rck peptides showed that the peptide containing amino acids 140-150 promoted adhesion, whereas amino acids between 150 and 159 modulated invasion. Expression of dominant-negative derivatives and use of specific inhibitors demonstrated the crucial role of small GTPases Racl and Cdc42 in activating the Arp2/3 complex to trigger formation of actin-rich accumulation, leading to Rck-dependent internalization. Finally, scanning and transmission electron microscopy with Rck-coated beads and E. coli expressing Rck revealed microvillus-like extensions that formed a Zipper-like structure, engulfing the adherent beads and bacteria. Overall, our results provide new insights into the Salmonella T3SS-independent invasion mechanisms and strongly suggest that Rck induces a Zipper-like entry mechanism. Consequently, Salmonella seems to be the first bacterium found to be able to induce both Zipper and Trigger mechanisms to invade host cells.
基金supported by National Marine Public Welfare Scientific Research Project of China (No.201105029)the National Key Technology S&D Program (No.2012BAD33B07)Program for Changjiang Scholars and Innovative Research Team in University (IRT1188)
文摘Sea cucumber and cordyceps sinensis are used as both food and traditional medicines in Asia. This study was carried out in order to investigate the hpyerglycemic effect of a mixture of sea cucumber （Apostichopusjaponicas） and cordyceps sinensis （Cor-dyceps militaris） （SCC） in diabetic rat and explore the mechanism underlining such an effect. The diabetic model rat was induced with intraperitoneal injection of streptozotocin （STZ）. The diabetic model rats were randomly divided into control group （0.9% NaC1）, low dose group （300 mg SCC.（kg body weight）-1） and high dose group （1200 mg SCC （kg body weight）-l）. Sodium chloride and SCC were intragastrically administered once a day for 35 d. Changes in fasting serum glucose and serum insulin content, oral glucose tolerance and liver and muscle glycogen content were routinely evaluated. Pancreas tissue and β-cells of islets were observed under both optical and transmission electronic microscope, respectively. The abundance of glucose metabolism-relating genes in gastrocnemius and epididymal adipose tissue was determined with either reverse transcription PCR （RT-PCR） or western blotting. Results showed that SCC significantly decreased fasting serum glucose content, improved glucose tolerance and increased serum insulin and glycogen content; repaired STZ-injured β-cells of diabetic rat, and increased the expression of phosphatidylinositol 3 kinase （PI（3）K）, protein kinase B （PKB） and glucose transporter 4 （Glut4） encoding protein in both gastroenemius and adipose tissue, and Glut4 encoding gene in peripheral tissue. Our findings demonstrated that SCC exerted an anti-hyperglycemic effect by repairing β-cells and promoting insulin-mediated signal transduction pathway in insulin-sensitive gastrocnemius and adipose tissue.
文摘Microbiological investigation of the fossil animals preserved in permafrost represents obvious interest for science. Lack of data in this sphere gives even greater importance to any findings giving us opportunity to learn more about remote past of microorganisms. In this respect, preserved remains of fossil are considered as unique biological materials for scientific investigations. Bacillus bacteria strains isolated from the paleomicrobiota of mammoth fauna are not only have high durability （20-30 thousand of years） in permafrost, but are still able to produce biologically active substances. Strains of bacteria of the genus Bacillus, isolated from the tissues of the representatives of the mammoth fauna have strong antagonistic properties to hemolytic streptococci--Streptococcus equi, pathogenic for animals--Salmonella abortus equi, also toxigenic micromycetes genera Aspergillus, Alternuria, Penicillum and fungal pathogens of plant diseases--Botrytis cimeria and Fuzarium oxysporium. The strains of bacteria of the genus Bacillus are not pathogenic to plants and animals, but initially resistant to wide range of antibiotics. Dominance strains of Bacillus bacteria, producing strong bacteriocins in the soft tissues of fossil animals, contributing to their long cryo bio conservation. In addition, bacterial strains of Bacillus subtilis, isolated from paleo microbiota have strong oxidizing properties. Microbiota of fossils preserved in permafrost of Yakutia is of particular interest for microbiology and modem biotechnology.
文摘Based on rpoB gene micro array as target gene, we are going to use gene chip technology to test 24 mycobacterium standard specimens, 8 non-mycobacterium specimens and 86 mycobacterium clinical isolated specimens. As a result, after mycobacterium and non-mycobacterium standard specimens were duplicated by PCR, mycobacterium standard specimens reproduced 360bp DNA fragments; on the other hand, non-mycobacterium specimens did not reproduce any fragments except for hemolytic streptococcus and corynebacterium pseudodiphtheriticum which had the same results as mycobacterium standard specimens. Sensitive test is able to detect lpg tuberculosis mycobacterium DNA. The probe test showed that, among 21 oligonucleotide probes, probe-M. fortuitum and M. marinum were cross-hybrid; the other probes were specific. We used the new method to identify 126 mycobacterium clinical isolated specimens. The test results of this new method matched with conventional method. In conclusion, compared to the traditional method, the use of rpob gene chip technology to identify mycobacterium species will be faster, more accurate and higher value in application.
文摘Objective： Diabetic nephropathy （DN） is one of the most common causes of end-stage renal failure. The pathogenesis of progressive renal injury is multifactorial and the mechanism by which hyperglycemia causes microangiopathy is still poorly understood. The WNT pathway is activated in DN and regulating β-catenin protein levels is referred to as the canonical Wntβ-catenin pathway. Because the renin angiotensin system has been reported to be an important contributory factor in the pathophysiology of DN, exogenous administration of angiotensin Ⅱ receptor antagonist may be beneficial in counteracting some biochemical or functional changes of DN. The aim of the study was to determine the β-catenin expression and the possible protective effects of irbesartan, an angiotensin Ⅱ type 1 receptor blocker （ARB） in a rat model of streptozotocin（STZ）-induced diabetic nephropathy. Methods： STZ-induced DN in rats was assessed biochemically by measuring urine volume, protein and creatinine clearance as well as Kidney weight/body weight （KW/BW） and the index of mesangial expansion. Three groups of male Sprague-dawley rats were used. The first group consisted of non-diabetic control rats （control）. The second group was the untreated diabetic rats（STZ＋vehicle）. The third group consisted of diabeti rats treated with irbesartan, 50 mg/kg for 12 weeks （STZ＋irbesartan）. Immunohistochemical stainings and real time PCR for β-catenin were performed in renal cortex of rat modals. Results： Marked hyperglycemia, polyuria, proteinuria, renal hypertrophy, mesangial matrix expansion and glomerular hyperfiltration were observed in STZ diabetic rats. The levels of microalbuminuria and KW/BW in the STZ＋irbesartan group were lower than those in the STZ＋vehicle group （P〈0.05）. The up-regulated immunostaining and mRNA expression of β-catenin were decreased in renal cortic of the Irbesartan-treated diabetic group, but there was no significant difference compared to the untreated diabetic group. Conclusion： The data suggest that irbesartan ameliorates proteinuria and renal hypertrophy, charactered damages of STZ-induced early-stage DN in rats, but its effective drug target is not to inhibit the up-regulated expressions of β-catenin.
文摘The aim of this study was to investigate the antihyperglycemic and antioxidant effects of Po （Portulaca oleracea） lyophilised aqueous extract in diabetic male Wistar rats. Diabetes was induced intraperitonially by a single injection of STZ （streptozotocin） （60 mg/kg bw （body weight））. Twenty diabetic rats, weighing 263 ± 5 g, were divided into two groups fed a casein diet supplemented or not with Po extract （1 g/kg bw）, for four weeks. Control group （n = 6） received 0.23-0.25 mL of citrate buffer and was fed a standard diet during the experiment. The study was carried out at Oran University, Algeria and the entire experiments lasted from September 2011 to July 2012. Blood was obtained from the abdominal aorta of rats after fasting overnight and standard methods were used for the extraction of spices, determination of glycemia, insulinemia, lipid peroxidation and antioxidant enzymes activities. Portulaca oleracea treated compared to untreated rats, glycemia and HbAIc values were respectively 2.8- and 1.7-fold lower. TBARS （thiobarbituric acid reactive substances） concentrations were reduced in RBC （red blood cells） （-54%） and plasma （-65%）. Moreover, in liver and kidney, TBARS values were respectively 1.8- and 2-fold lower. SOD （superoxide dismutase） and GSH-Px （glutathione peroxidase） activities were increased respectively by ＋38% and ＋85%, in liver. GSSG-Red （glutathione reductase） activity was 1.9-fold higher in kidney, while CAT （catalase） was improved in kidney （＋48%）. In RBCs, SOD, GSH-Px, GSSH-Red and CAT activities were increased by 31%, 42%, 56% and ＋50%, respectively. These data have cast a new light on the actions of Portulaca oleracea and its antioxidant potential benefits in preventing diabetes and its complications.
文摘Objective To set up a rapid and simple method for identificating bacteria by 16S 23SrDNA intergenic spacer regions (ISRs) Methods Polymorphic products of PCR from ISRs were selected on agarose gel and sequenced directly using purified fragments by excising the gel without cloning Nucleotide sequences were compared with GenBank databases and analyzed by DNAMAN program Results There was only a single product in streptococcus genus after PCR amplification of 16S 23SrDNA ISRs Five streptococcal species were obtained from 7 strains of streptococcus Two major amplicons were consistently generated for 8 strains of Haemophilus influenzae (H influenzae) The sequence data showed that they all belonged to H influenzae type b on GenBank databases Conclusion PCR and direct sequencing of 16S 23SrDNA ISRs were very successful methods for bacterial species identification
基金Supported by Ministry of Education of the People's Republic of China:a Mechanism Study of Electro-Acupuncture EX-B3's Effect on Pancreas PDX-1 of Goto-Kakizaki Rats(No.313010)
文摘OBJECTIVE: To investigate the effect of low frequency electro-pulse acupuncture(EA) on blood glucose in rats with streptozotocin-induced type 2diabetes, and the possible mechanism underlying the action.METHODS: Rat models were established with high fat feeding and intraperitoneal injection of streptozotocin(STZ)(30 mg/kg). Rats with a random blood glucose > 16.7 mmol/L and blood glucose at 2 h-point of oral glucose tolerance test(OGTT) > 11.1 mmol/L were included as diabetic rats, and randomly divided into model group, EA Weiwanxiashu(EX-B 3) group, EA Zusanli(ST 36)group, glimepiride group, and EA non-acupoint group(n = 12). EA(2 Hz continuous wave, 2 m A,20 min/day, 6 days/week, 4 weeks) and intra-gastric administration of glimepiride were applied as interventions. With fasting blood glucose and OGTT tested at the end of the intervention, thestudy observed the patterns of hypoglycemic effects. For mechanism study, it observes hematoxylin and eosin staining and Masson staining of pancreas paraffin sections, protein expression of glucagon-like peptide 1 receptor(GLP-1R) in the pancreas and skeletal muscle, glucose transporter 4(GLUT4) protein expression in skeletal muscle membrane, to detect whether EA controls blood glucose via regulation of GLP-1R.RESULTS: EA Weiwanxiashu(EX-B 3) significantly increased model rats' pancreas GLP-1R, and GLUT4 of skeletal muscle membrane; the therapy significantly decreased model rats' skeletal muscle GLP-1R, restored pancreas morphology, and reduced fasting blood glucose and insulin resistance indices.CONCLUSION: EA Weiwanxiashu(EX-B 3) alone has significant effect on glycemia. EA Weiwanxiashu(EX-B 3) plus glimepiride further strengthen the effect. The regulation of the GLP-1R in pancreas and skeletal muscle might be mechanism underpinning the effect.
