Two transgenic mouse strains , in which the expression of human factor IX (hFIX) in the milk were different significantly, were bred, and the foreign gene integration as well as the content of hFIX in the milk were de...Two transgenic mouse strains , in which the expression of human factor IX (hFIX) in the milk were different significantly, were bred, and the foreign gene integration as well as the content of hFIX in the milk were detected by PCR, Southern blot, FISH and ELISA, respectively. The results showed that approximately 50% offsprings were transgenic positive. Foreign gene integrated in mouse chromosomes was intact. The hFIX expression of each mouse in the same strain was different, the content of hFIX in the milk was (43.32±5.41)μg/mL in FIX-33 transgenic strain and (1.16±0.45)μg/mL in FIX-124 transgenic strain. Meanwhile, the hFIX gene expression between the two strains was different remarkably (P<0.01). We conclude that the characteristics of inheritance and expression in the founder were able to be transferred to their offsprings stably.展开更多
基金国家"8 6 3"高技术项目基金资助 (No .2 0 0 1AA2 130 11)上海市现代生物与新药产业发展基金资助 (No .9943190 4)上海市卫生局青年基金资助 (No.0 0 4Y10 )~~
文摘Two transgenic mouse strains , in which the expression of human factor IX (hFIX) in the milk were different significantly, were bred, and the foreign gene integration as well as the content of hFIX in the milk were detected by PCR, Southern blot, FISH and ELISA, respectively. The results showed that approximately 50% offsprings were transgenic positive. Foreign gene integrated in mouse chromosomes was intact. The hFIX expression of each mouse in the same strain was different, the content of hFIX in the milk was (43.32±5.41)μg/mL in FIX-33 transgenic strain and (1.16±0.45)μg/mL in FIX-124 transgenic strain. Meanwhile, the hFIX gene expression between the two strains was different remarkably (P<0.01). We conclude that the characteristics of inheritance and expression in the founder were able to be transferred to their offsprings stably.
