目的比较测试片法(test piece method,TP)、计数琼脂平板法(counting Agar plate method,CAP)及琼脂倾注TTC平板法(2,3,5-chloride three phenyl tetrazole,TTC)对食品微生物菌落总数的测定效果。方法选取70例需进行菌落总数测定的食品...目的比较测试片法(test piece method,TP)、计数琼脂平板法(counting Agar plate method,CAP)及琼脂倾注TTC平板法(2,3,5-chloride three phenyl tetrazole,TTC)对食品微生物菌落总数的测定效果。方法选取70例需进行菌落总数测定的食品样本作为研究对象,分别应用测试片法、计数琼脂平板法及琼脂平板法进行测定,对比三种方法的菌落总数超标样品检出率。结果 TTC法菌落总数超标检出率为24.3%,显著高于测试片法(7.1%)及计数琼脂平板法(11.4%),P<0.05。结论与TP及CAP相比,TTC法对于菌落总数超标样品的检出率更高、灵敏度更好。展开更多
The aim of this study is to assess the water microbiologic pollution in Durres＇s Harbour basin and to compare it with European standards. The comparison of heterotrophs and total coliforms level in sampling are the e...The aim of this study is to assess the water microbiologic pollution in Durres＇s Harbour basin and to compare it with European standards. The comparison of heterotrophs and total coliforms level in sampling are the essence of this research. The object of this study is done in four sampling areas of Durres＇s Harbour basin. In order to compare the level of water microbiologic pollution in four areas of Durres＇s Harbour basin, control area is also studied which is a beach area near the Harbour named Apollonia Beach. The sampling areas were： Ferry Terminal （FT）, Fishery Harbor （FH）, East Zone （EZ）, Fuel Quay （FQ） and Apollonia Beach （AB）, respectively. The period of sample-taking was July-October 2008. The strategy used for this purpose consisted in water insemination with coverage method by means of Petri＇s plates according to respective dilutions in culture media Plate Count Agar （PCA） for heterotrophs and MacConkey for total coliforms. The number of colonies that are formed determines the number of cells at the moment of water insemination, the number of heterotrophs in culture media PCA, respectively. The number of pink and red colonies that were formed determines the number of cells at the moment of water insemination, the number of total coliforms in culture media MacConkey, respectively. The measure ofheterotrophic bacteria and total coliforms used is Colony-Forming Units （CFU）/100 mL seawater. （AB） is within European standards. The richest area with heterotrophs is （FH）, which confirms the fact that it is the most polluted microbiologic area in the Harbour basin of Durres. The richest area with total coliforms is （FT）. The poorest area with heterotrophs and total coliforms is （FQ）.展开更多
文摘目的比较测试片法(test piece method,TP)、计数琼脂平板法(counting Agar plate method,CAP)及琼脂倾注TTC平板法(2,3,5-chloride three phenyl tetrazole,TTC)对食品微生物菌落总数的测定效果。方法选取70例需进行菌落总数测定的食品样本作为研究对象,分别应用测试片法、计数琼脂平板法及琼脂平板法进行测定,对比三种方法的菌落总数超标样品检出率。结果 TTC法菌落总数超标检出率为24.3%,显著高于测试片法(7.1%)及计数琼脂平板法(11.4%),P<0.05。结论与TP及CAP相比,TTC法对于菌落总数超标样品的检出率更高、灵敏度更好。
文摘The aim of this study is to assess the water microbiologic pollution in Durres＇s Harbour basin and to compare it with European standards. The comparison of heterotrophs and total coliforms level in sampling are the essence of this research. The object of this study is done in four sampling areas of Durres＇s Harbour basin. In order to compare the level of water microbiologic pollution in four areas of Durres＇s Harbour basin, control area is also studied which is a beach area near the Harbour named Apollonia Beach. The sampling areas were： Ferry Terminal （FT）, Fishery Harbor （FH）, East Zone （EZ）, Fuel Quay （FQ） and Apollonia Beach （AB）, respectively. The period of sample-taking was July-October 2008. The strategy used for this purpose consisted in water insemination with coverage method by means of Petri＇s plates according to respective dilutions in culture media Plate Count Agar （PCA） for heterotrophs and MacConkey for total coliforms. The number of colonies that are formed determines the number of cells at the moment of water insemination, the number of heterotrophs in culture media PCA, respectively. The number of pink and red colonies that were formed determines the number of cells at the moment of water insemination, the number of total coliforms in culture media MacConkey, respectively. The measure ofheterotrophic bacteria and total coliforms used is Colony-Forming Units （CFU）/100 mL seawater. （AB） is within European standards. The richest area with heterotrophs is （FH）, which confirms the fact that it is the most polluted microbiologic area in the Harbour basin of Durres. The richest area with total coliforms is （FT）. The poorest area with heterotrophs and total coliforms is （FQ）.
