[Objective] The experiment aimed to study chromatographic fingerprint in volatile components of acacia honey and provide scientific evaluation and effective control on quality of acacia honey.[Method] Using solid-phas...[Objective] The experiment aimed to study chromatographic fingerprint in volatile components of acacia honey and provide scientific evaluation and effective control on quality of acacia honey.[Method] Using solid-phase microextraction method to separate and detect volatile components and construct chromatographic fingerprint.[Result] The honey was preheated for 15 min in water bath at 40 ℃ and solid-phase microextraction 85 μmPA was used to extract in overhead air about 30 min,then put it into the injector and desorpted 3 min,which is in 230 ℃.The Supelco WaxTM10 30 m×0.25 mm×0.25 μm column and gradient heating program was the best method to separate volatile components from honey.83 fingerprint peaks were constructed,among which 17 common fingerprint peaks were comprised of chromatographic fingerprint of volatile components of acacia honey.[Conclusion] The chromatographic fingerprint could provide reference for quality control of acacia honey.展开更多
The identification of germplasm is an important step for effective utilization of the available germplasm. In previous studies, isoenzyme, RAPD and SSR techniques had been used to conduct the genetic identification of...The identification of germplasm is an important step for effective utilization of the available germplasm. In previous studies, isoenzyme, RAPD and SSR techniques had been used to conduct the genetic identification of watermelon ( Citrullus lanatus (Thunb.) Mansf.), but their effectiveness was limited due to the extremely narrow genetic background among watermelon genotypes. In this research, amplified fragment length polymorphism (AFLP), which was reported as a reliable technique with high efficiency in detecting polymorphism, was used to conduct genetic analysis and variety identification of thirty genotypes of watermelon core collection that represent a wide range of breeding and commercially available germplasm. As a result, a DNA fingerprint based on 15 bands amplified with four primer combinations was developed. In this fingerprint, each genotype has its unique fingerprint pattern and can be distinguished from each other. Furthermore, in or der to facilitate the utilization of AFLP marker in practice, one specific AFLP band of genotype 'PI296341' coming from fragment amplified by primer combination E-AT/M-CAT was successfully converted into a sequence characterized amplified region (SCAR) marker.展开更多
Polymorphism of nine strains （CF05, CF09, 29, 916, AU9, Chang10, Chang7, 8808 and AU. Japanese） of A. auricular cultivated in Heilongjiang Province were analyzed by RAPD （Random Amplication polymorphic DNA）. Thirt...Polymorphism of nine strains （CF05, CF09, 29, 916, AU9, Chang10, Chang7, 8808 and AU. Japanese） of A. auricular cultivated in Heilongjiang Province were analyzed by RAPD （Random Amplication polymorphic DNA）. Thirteen primers were selected from forty PCR primers with 10bp long random primer. The results showed that nine strains of A. auricular have a high level of genetic diversity and the percentage of DNA polymorphic was 96.05. The genotypes of 9 strains of Auricularia auricular were identified by the fingerprints from primer 27 and primer 46 by RAPD analysis. The results are helpful for quickly identifying strains of A. auricular in its early breeding time, and also provides a powerful theoretic basis to differentiate strains （Auricularia auricular） whose morphology is very similar in breeding programs of edible fungus.展开更多
The inheritance of chloroplast DNA (cpDNA) in sweet potato (Ipomoea batatas Lain.) was analyzed using DNA restriction fingerprinting. The cpDNA fingerprints of hybrids from reciprocal crosses between Xushu18 and AB78-...The inheritance of chloroplast DNA (cpDNA) in sweet potato (Ipomoea batatas Lain.) was analyzed using DNA restriction fingerprinting. The cpDNA fingerprints of hybrids from reciprocal crosses between Xushu18 and AB78-1 were found to be identical to those of their female parents, which reveals that cpDNA of sweet potato is maternally inherited in this intervarietal crossing. This maternal cpDNA transmission pattern does not accord with the putative one based on former cytological studies. The plastid inheritance in Convolvulaceae has been briefly reviewed in this study, and the utility of DNA restriction fingerprinting analysis in the study of plastid inheritance is also discussed.展开更多
Brood parasitism and egg mimicry of Himalayan Cuckoo（Cuculus saturatus） on its host Blyth＇s Leaf Warbler（Phylloscopus reguloides） were studied in south-western China from April to July 2009.The cuckoo laid a whit...Brood parasitism and egg mimicry of Himalayan Cuckoo（Cuculus saturatus） on its host Blyth＇s Leaf Warbler（Phylloscopus reguloides） were studied in south-western China from April to July 2009.The cuckoo laid a white egg with fine brown markings on the blunt end.The eggs were conspicuously bigger than the host＇s own,with 2.06 g in mass and 1.91 cm3 in volume.Visual modeling showed that the cuckoo eggs,which from the human eye appeared to mimic the host eggs to a great extent,were completely different from the host eggs in both hue and chroma.The characters of the Himalayan Cuckoo nestling,reported for the first time,included two triangular and black patches on its gape,which appeared from four days old and became darker with age and growth.While this character also exists in nestlings of Oriental Cuckoo（C.optatus）,it has not been found for other Cuculus species.Our results reveal cryptic aspects in the cuckoo-host egg color matching,which are not visible to the naked human eye,and indicate that high mimetic cuckoo eggs rejected by hosts,as determined by human observers in previous studies,might not be mimetic as birds see them.展开更多
DNA fingerprinting among members of the Chinese drug Pu Gong Ying(Taraxacum mongolicum Hand,-Mazz.)and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction(PCR)including arbit...DNA fingerprinting among members of the Chinese drug Pu Gong Ying(Taraxacum mongolicum Hand,-Mazz.)and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction(PCR)including arbitrarily primed polymerase chain reaction(AP-PCR)and random amplified polymorphic DNA(RAPD).Distinctive,reproducible genomic fingerprints from DNA from 7 species belonged to Compositae were generated with two long(20 and 24 mer)and one short(10 mer)randomly chosen primers.The Pu Gong Ying can be differentiated from six species of Tu Gong Ying according to the banding pattems of their amplified DNA on agarose gels.The results showed that AP-PCR and RAPD methods can be used for identifying Chinese drugs.Moreover,the Similarity Indexes of the genomic DNA fingerprints showed that Pu Gong Ying and its adulterants are unrelated.Therefore,AP-PCR and RAPD methods can be used for identifying Chinese drugs.展开更多
To study the distribution of 25 elements, i.e. Be, Cd, Ce, Cr, Cu, Dy, Er,Eu, Gd, Ge, Ho, La, Lu, Mo, Nd, Pb, Pr, Sm, Sr, Tb, Tl, Tm, Y, Yb and Zn in Phytolacca, atraditional Chinese medicinal herb, collected from nin...To study the distribution of 25 elements, i.e. Be, Cd, Ce, Cr, Cu, Dy, Er,Eu, Gd, Ge, Ho, La, Lu, Mo, Nd, Pb, Pr, Sm, Sr, Tb, Tl, Tm, Y, Yb and Zn in Phytolacca, atraditional Chinese medicinal herb, collected from nine areas of P. R. China. Methods Twenty-fiveelements in Phytolacca including essential elements, toxic elements and rare earth elements intraditional Chinese medicinal herbs from different areas were analyzed by ICP-MS. Results The 25elements in Phytolacca were determined by ICP-MS under optimized conditions. The detection limitswere 0.003 -0.71 ng·mL^(-1). The recoveries were 88% - 118% . The relative standard deviations ofthe measurements were 1.7% - 13.3%. Conclusion The determined concentrations of elements inPhytolacca acinosa Roxb vary from one area to another; however, the distribution tendency ofelements in all the samples is similar. The distribution tendency of rare earth elements inPhytolacca acinosa Roxb is consistent with that in nature.展开更多
[Objective] The genetic diversity of major mango cultivars in China was analyzed by using SSR markers, and their fingerprints were constructed so as to provide theoretical basis for germplasm innovation and breeding o...[Objective] The genetic diversity of major mango cultivars in China was analyzed by using SSR markers, and their fingerprints were constructed so as to provide theoretical basis for germplasm innovation and breeding of mango. [Method] With 115 pairs of SSR primers, genetic diversity analysis and cluster analysis were performed for 30 mango cultivars, among which the genetic relationships were analyzed. [Result] Total 64 pairs of polymorphic primers were screened out from the 115 pairs of primers, and total 343 bands were amplified from the 30 cultivars with 73.2% of polymorphic bands. On average, 3.9 allelic loci were detected for each pair of primers with genetic diversity index of 0.5, Shannon＇s diversity index of 1.00 and polymorphism information content of 0.49, indicating higher genetic diversity. The cluster analysis showed that the 30 major cultivars could be classified into four categories. The first category included 14 cultivars; the second category included 11 cultivars, most of which were introduced from abroad; the third category included 4 cultivars, Le., Miansan, Parayinda, Baiyu and Hongxiangya： the fourth category included only one cultivar Maqiesu.By using 7 pairs of SSR markers, i.e., M42, M49, M54, M55, M96, M99 and M103, digital fingerprints were constructed for the 30 mango cultivars. [Conclusion] The 30 mango cultivars present more complex genomic genetics and abundant genetic information, and they have higher genetic diversity.展开更多
With western Jilin Province as the study region, spectral characteristics and texture features of remote sensing images were taken as the classification basis to construct a Decision Tree Model and extract information...With western Jilin Province as the study region, spectral characteristics and texture features of remote sensing images were taken as the classification basis to construct a Decision Tree Model and extract information about settlements in western Jilin Province, and the manually-extracted information about settlements in western Jilin Province was evaluated by confusion matrix. The results showed that Decision Tree Model was convenient for extracting settlements information by integrating spectral and texture features, and the accuracy of such a method was higher than that of the traditional Maximum Liklihood Method, in addition, calculation methods of extracting settlements information by this mean were concluded.展开更多
Aim To establish a method for determination of Ginkgo biloba L, its extractand preparations with HPLC fingerprints, so as to control the quality of the preparations. MethodsHPLC-DAD method was used to determine the co...Aim To establish a method for determination of Ginkgo biloba L, its extractand preparations with HPLC fingerprints, so as to control the quality of the preparations. MethodsHPLC-DAD method was used to determine the constituents in preparations. Diamonsil? C_(18) (200mm X 4.6 mm, 5 μm) was used as analytical column, and acetonitrile/KH_2PO_4 was used as mobilephase with gradient elu-tion. The column temperature was at 24 ℃. The HPLC profile of chemicalconstituents of control sample and preparations were analyzed using similarity software. Results Thefingerprints of different preparations from different companies were slightly different because ofthe different preparing procedures. Mean while, the fingerprints of different batches of the samepreparation from the same company were similar to each other and the technology of each preparationwas stable. Conclusion This method is accurate, reproducible , simple, and can be used as ananalytical method for the routine quality control of Ginkgo biloba preparations.展开更多
[Objective] This research aimed to develop molecular identification method for Castanopsis hystrix,Castanopsis carlesii and Quercus griffithii.[Method] DNA fingerprints of C.hystrix,C.carlesii and Q.griffithii were es...[Objective] This research aimed to develop molecular identification method for Castanopsis hystrix,Castanopsis carlesii and Quercus griffithii.[Method] DNA fingerprints of C.hystrix,C.carlesii and Q.griffithii were established by using ISSR-PCR method.Cluster Analysis was carried out by using UPGMA method based on Nei＇s genetic distances among each individual.[Result] Six polymorphic primers were selected from 50 ISSR primers for ISSR-PCR amplification,and totally 86 discernible DNA bands were amplified with 53 polymorphic bands,accounting for 61.2% of the total.The average number of DNA bands amplified by each primer was 10.75.Specifically,totally 5 primers had amplified differential bands and specific bands,which were able to accurately identify C.hystrix,C.carlesii and Q.griffithii.As calculated by DPS v3.01 software,the genetic distances among test materials were ranged from 0.166 67 to 0.809 52,with an average of 0.563 57.[Conclusion] ISSR-PCR method can be used to identify C.hystrix,C.carlesii and Q.griffithii effectively.展开更多
[Objective] The aim of this study was to provide a basis for distinguishing quality of rhubarb in different production areas. [Method ] X-ray diffraction patterns of rhubarbs in different production areas of Qinghai w...[Objective] The aim of this study was to provide a basis for distinguishing quality of rhubarb in different production areas. [Method ] X-ray diffraction patterns of rhubarbs in different production areas of Qinghai were obtained by X-ray diffraction analysis, and then its similarity analysis was also investigated. [ Result] The content of chemical components in rhubarbs from different production areas had differences, but its diffraction patterns and diffraction peaks had certain fingerprint characteristics. [ Conclusion] X-ray diffraction method is a fast and effective method for identifying rhubarb and other Chinese herbal medicines in different production areas.展开更多
Aim To study the correlation between the HPLC fingerprints and in vitro antibacterial activities of EtOAc extracts of Radix isatidis from various sources. Methods Ten batches of Radix isatidis EtOAc extracts were anal...Aim To study the correlation between the HPLC fingerprints and in vitro antibacterial activities of EtOAc extracts of Radix isatidis from various sources. Methods Ten batches of Radix isatidis EtOAc extracts were analyzed with HPLC and the fingerprints were established. The influence of EtOAc extracts on the thermogenic curve of growth of Escherchia coli was obtained by microcalorimetry. The chemical differences of EtOAc extracts of Radix isatidis from various sources in the HPLC fingerprints were probed with hierarchical clustering analysis and similarity analysis. The correlation between the HPLC fingerprints and in vitro antibacterial activities was analyzed with multivafiant correlation analysis. Results Close correlation existed between the HPLC fingerprints and in vitro antibacterial activities of EtOAc extracts of Radix isatidis. Conlusion The combination of HPLC fingerprints and antibacterial activities can be used to discover principle components of Radix isatidis on bioactivity.展开更多
The flavonoids is a kind of complex compound and quercetin is a main composition in rape pollen.Taking quercetin as a sample and using separation technology and high performance liquid chromatography,chromatography fi...The flavonoids is a kind of complex compound and quercetin is a main composition in rape pollen.Taking quercetin as a sample and using separation technology and high performance liquid chromatography,chromatography fingerprint technique of Chinese medicine is introduced in food field to construct chromatography fingerprint of rape pollen,which can be used for medicinal use and edible use.The method will become a beneficial supplement for quality control method to ensure the safety and effectiveness of products.展开更多
In the present study, the nutritional compositions of maca which was grown in a mountain area at an elevation of 2 200-2 800 m of Ebian County,Sichuan Province were measured, and then HPLC analysis on two representati...In the present study, the nutritional compositions of maca which was grown in a mountain area at an elevation of 2 200-2 800 m of Ebian County,Sichuan Province were measured, and then HPLC analysis on two representative active compounds（macaene and macamide） in the maca sample was performed.The results revealed that there were 24.20% total protein, 18.40% total amino acids（including 3.84% arginine）, 42.80% total sugars, 1.36% fat and kinds of minerals（including 1.14% potassium） in Ebian maca. HPLC fingerprints of macaene and macamide of Ebian maca were similar to those of Peru maca. The results suggested that maca could be cultivated with good quality in some mountain areas with an altitude1 000 m lower than the origin place in Peru.展开更多
By analyzing the contents of inorganic elements in Radix Pseudostellariae from Guizhou Province, the aim was to assess the present quality of Radix Pseu- dostellariae, set limited standards of heavy metals, establish ...By analyzing the contents of inorganic elements in Radix Pseudostellariae from Guizhou Province, the aim was to assess the present quality of Radix Pseu- dostellariae, set limited standards of heavy metals, establish element fingerprints, and find out the characteristic elements. ICP-MS was used to measure the content of inorganic elements and map the element fingerprints. Additionally, WM/T2-2004 was applied to evaluate the quality of heavy metal elements, and the characteristic elements were determined by principal component analysis. The results showed that the contents of inorganic elements in Radix Pseudostellariae were between 0.057 and 959 mg/kg with the coefficients of variation ranging from 0.134 to 1.478, and the contents of Cd, As, Pb, and Hg were below the Standard of WM/T2-2004 in 90% of Radix Pseudostellariae. The standard limits of heavy metals in Radix Pseu- dostellariae were Cr≤6.5 mg/kg, Cu≤10 mg/kg, As≤2.0 mg /kg, Cd≤0.3 mg/kg, Hg≤0.15 mg/kg, and Pb≤4.0 mg/kg. The features of the inorganic eJements finger- prints could provide theoretical basis of identifying the quality of Radix Pseudostel- lariae and distinguishing Radix Pseudostellariae from other Chinese herbal medicines. The characteristic inorganic elements of Radix Pseudostellariae were found to be Cd, Cu, Co, Zn, Fe, Ca, Mg, and AI. Radix Pseudostellariae from Guizhou Province contained abundant inorganic elements, and the contents of heavy metals were below the evaluation criterion. The study provided a reference for the future development of the limiting values of heaw metals in Radix Pseudostellariae.展开更多
This paper reports the rationale and methods of DNA extraction and purification from nine species of Compositae and four commercial drugs of corresponding plant Elephantopus scaber. The comparison of three methods: Cs...This paper reports the rationale and methods of DNA extraction and purification from nine species of Compositae and four commercial drugs of corresponding plant Elephantopus scaber. The comparison of three methods: CsCl gradient, CTAB/CsCl gradient and CTAB miniprep extraction by yield, purity and factors affecting PCR was carried out. In conclusion, CTAB miniprep method provides a rapid, effective, economic approach for isolating genomic DNA for Chinese drug identification by genomic fingerprints.展开更多
Aim To establish a method for differentiating commercial samples of Panax species including notoginseng, cultivated ginseng （Chinese ginseng and Korean ginseng）, wild ginseng, red ginseng, three types of American gi...Aim To establish a method for differentiating commercial samples of Panax species including notoginseng, cultivated ginseng （Chinese ginseng and Korean ginseng）, wild ginseng, red ginseng, three types of American ginsengs, and one American ginseng preparation with their HPLC fingerprints for assnrning the quality of different commercial samples of Panax species. Methods HPLC-UV method was used to establish their fingerprints, Zorbax Extend C18 （250 mm×4.6 mm, 5 μm） was used as the analytical column, and acetonitrile/KH2PO4 aqueous solution was used as the mobile phase with gradient elution. Results The fingerprints of different commercial samples of Panax species varied in their holistic chromatograms and some specific constituents. Conclusion This method is reliable, reproducible and simple, It could be applied in the routine authentication of different commercial samples of Panax species展开更多
基金Support by Department of Education Science and Technology Research Projects of Hebei Province(2008310)the National Special Fund for the Commonweal Industry(200810345)~~
文摘[Objective] The experiment aimed to study chromatographic fingerprint in volatile components of acacia honey and provide scientific evaluation and effective control on quality of acacia honey.[Method] Using solid-phase microextraction method to separate and detect volatile components and construct chromatographic fingerprint.[Result] The honey was preheated for 15 min in water bath at 40 ℃ and solid-phase microextraction 85 μmPA was used to extract in overhead air about 30 min,then put it into the injector and desorpted 3 min,which is in 230 ℃.The Supelco WaxTM10 30 m×0.25 mm×0.25 μm column and gradient heating program was the best method to separate volatile components from honey.83 fingerprint peaks were constructed,among which 17 common fingerprint peaks were comprised of chromatographic fingerprint of volatile components of acacia honey.[Conclusion] The chromatographic fingerprint could provide reference for quality control of acacia honey.
文摘The identification of germplasm is an important step for effective utilization of the available germplasm. In previous studies, isoenzyme, RAPD and SSR techniques had been used to conduct the genetic identification of watermelon ( Citrullus lanatus (Thunb.) Mansf.), but their effectiveness was limited due to the extremely narrow genetic background among watermelon genotypes. In this research, amplified fragment length polymorphism (AFLP), which was reported as a reliable technique with high efficiency in detecting polymorphism, was used to conduct genetic analysis and variety identification of thirty genotypes of watermelon core collection that represent a wide range of breeding and commercially available germplasm. As a result, a DNA fingerprint based on 15 bands amplified with four primer combinations was developed. In this fingerprint, each genotype has its unique fingerprint pattern and can be distinguished from each other. Furthermore, in or der to facilitate the utilization of AFLP marker in practice, one specific AFLP band of genotype 'PI296341' coming from fragment amplified by primer combination E-AT/M-CAT was successfully converted into a sequence characterized amplified region (SCAR) marker.
基金The research was supported by Science Foundation of Northeast Forestry University (2004)
文摘Polymorphism of nine strains （CF05, CF09, 29, 916, AU9, Chang10, Chang7, 8808 and AU. Japanese） of A. auricular cultivated in Heilongjiang Province were analyzed by RAPD （Random Amplication polymorphic DNA）. Thirteen primers were selected from forty PCR primers with 10bp long random primer. The results showed that nine strains of A. auricular have a high level of genetic diversity and the percentage of DNA polymorphic was 96.05. The genotypes of 9 strains of Auricularia auricular were identified by the fingerprints from primer 27 and primer 46 by RAPD analysis. The results are helpful for quickly identifying strains of A. auricular in its early breeding time, and also provides a powerful theoretic basis to differentiate strains （Auricularia auricular） whose morphology is very similar in breeding programs of edible fungus.
文摘The inheritance of chloroplast DNA (cpDNA) in sweet potato (Ipomoea batatas Lain.) was analyzed using DNA restriction fingerprinting. The cpDNA fingerprints of hybrids from reciprocal crosses between Xushu18 and AB78-1 were found to be identical to those of their female parents, which reveals that cpDNA of sweet potato is maternally inherited in this intervarietal crossing. This maternal cpDNA transmission pattern does not accord with the putative one based on former cytological studies. The plastid inheritance in Convolvulaceae has been briefly reviewed in this study, and the utility of DNA restriction fingerprinting analysis in the study of plastid inheritance is also discussed.
基金supported by National Natural Science Foundation of China(3086004431071938)+1 种基金Program for New Century Excellent Talents in University(NCET-10-0111)China Postdoctoral Science Foundation(20110490967)funded project
文摘Brood parasitism and egg mimicry of Himalayan Cuckoo（Cuculus saturatus） on its host Blyth＇s Leaf Warbler（Phylloscopus reguloides） were studied in south-western China from April to July 2009.The cuckoo laid a white egg with fine brown markings on the blunt end.The eggs were conspicuously bigger than the host＇s own,with 2.06 g in mass and 1.91 cm3 in volume.Visual modeling showed that the cuckoo eggs,which from the human eye appeared to mimic the host eggs to a great extent,were completely different from the host eggs in both hue and chroma.The characters of the Himalayan Cuckoo nestling,reported for the first time,included two triangular and black patches on its gape,which appeared from four days old and became darker with age and growth.While this character also exists in nestlings of Oriental Cuckoo（C.optatus）,it has not been found for other Cuculus species.Our results reveal cryptic aspects in the cuckoo-host egg color matching,which are not visible to the naked human eye,and indicate that high mimetic cuckoo eggs rejected by hosts,as determined by human observers in previous studies,might not be mimetic as birds see them.
文摘DNA fingerprinting among members of the Chinese drug Pu Gong Ying(Taraxacum mongolicum Hand,-Mazz.)and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction(PCR)including arbitrarily primed polymerase chain reaction(AP-PCR)and random amplified polymorphic DNA(RAPD).Distinctive,reproducible genomic fingerprints from DNA from 7 species belonged to Compositae were generated with two long(20 and 24 mer)and one short(10 mer)randomly chosen primers.The Pu Gong Ying can be differentiated from six species of Tu Gong Ying according to the banding pattems of their amplified DNA on agarose gels.The results showed that AP-PCR and RAPD methods can be used for identifying Chinese drugs.Moreover,the Similarity Indexes of the genomic DNA fingerprints showed that Pu Gong Ying and its adulterants are unrelated.Therefore,AP-PCR and RAPD methods can be used for identifying Chinese drugs.
文摘To study the distribution of 25 elements, i.e. Be, Cd, Ce, Cr, Cu, Dy, Er,Eu, Gd, Ge, Ho, La, Lu, Mo, Nd, Pb, Pr, Sm, Sr, Tb, Tl, Tm, Y, Yb and Zn in Phytolacca, atraditional Chinese medicinal herb, collected from nine areas of P. R. China. Methods Twenty-fiveelements in Phytolacca including essential elements, toxic elements and rare earth elements intraditional Chinese medicinal herbs from different areas were analyzed by ICP-MS. Results The 25elements in Phytolacca were determined by ICP-MS under optimized conditions. The detection limitswere 0.003 -0.71 ng·mL^(-1). The recoveries were 88% - 118% . The relative standard deviations ofthe measurements were 1.7% - 13.3%. Conclusion The determined concentrations of elements inPhytolacca acinosa Roxb vary from one area to another; however, the distribution tendency ofelements in all the samples is similar. The distribution tendency of rare earth elements inPhytolacca acinosa Roxb is consistent with that in nature.
基金Supported by Natural Science Foundation of Hainan Province(34128)Fundamental Scientific Research Funds of Chinese Academy of Tropical Agricultural Sciences(1630032013031)~~
文摘[Objective] The genetic diversity of major mango cultivars in China was analyzed by using SSR markers, and their fingerprints were constructed so as to provide theoretical basis for germplasm innovation and breeding of mango. [Method] With 115 pairs of SSR primers, genetic diversity analysis and cluster analysis were performed for 30 mango cultivars, among which the genetic relationships were analyzed. [Result] Total 64 pairs of polymorphic primers were screened out from the 115 pairs of primers, and total 343 bands were amplified from the 30 cultivars with 73.2% of polymorphic bands. On average, 3.9 allelic loci were detected for each pair of primers with genetic diversity index of 0.5, Shannon＇s diversity index of 1.00 and polymorphism information content of 0.49, indicating higher genetic diversity. The cluster analysis showed that the 30 major cultivars could be classified into four categories. The first category included 14 cultivars; the second category included 11 cultivars, most of which were introduced from abroad; the third category included 4 cultivars, Le., Miansan, Parayinda, Baiyu and Hongxiangya： the fourth category included only one cultivar Maqiesu.By using 7 pairs of SSR markers, i.e., M42, M49, M54, M55, M96, M99 and M103, digital fingerprints were constructed for the 30 mango cultivars. [Conclusion] The 30 mango cultivars present more complex genomic genetics and abundant genetic information, and they have higher genetic diversity.
基金Supported by Financial Support of China Geological Survey(1212010916048)the Fundamental Research Funds for the Central Universities(200903046)~~
文摘With western Jilin Province as the study region, spectral characteristics and texture features of remote sensing images were taken as the classification basis to construct a Decision Tree Model and extract information about settlements in western Jilin Province, and the manually-extracted information about settlements in western Jilin Province was evaluated by confusion matrix. The results showed that Decision Tree Model was convenient for extracting settlements information by integrating spectral and texture features, and the accuracy of such a method was higher than that of the traditional Maximum Liklihood Method, in addition, calculation methods of extracting settlements information by this mean were concluded.
文摘Aim To establish a method for determination of Ginkgo biloba L, its extractand preparations with HPLC fingerprints, so as to control the quality of the preparations. MethodsHPLC-DAD method was used to determine the constituents in preparations. Diamonsil? C_(18) (200mm X 4.6 mm, 5 μm) was used as analytical column, and acetonitrile/KH_2PO_4 was used as mobilephase with gradient elu-tion. The column temperature was at 24 ℃. The HPLC profile of chemicalconstituents of control sample and preparations were analyzed using similarity software. Results Thefingerprints of different preparations from different companies were slightly different because ofthe different preparing procedures. Mean while, the fingerprints of different batches of the samepreparation from the same company were similar to each other and the technology of each preparationwas stable. Conclusion This method is accurate, reproducible , simple, and can be used as ananalytical method for the routine quality control of Ginkgo biloba preparations.
基金Supported by the Fundamental Research Fund in Guangxi Academy of Forestry " Population Genetics Study of Castanopsis hystrix"(Forestry 200901)~~
文摘[Objective] This research aimed to develop molecular identification method for Castanopsis hystrix,Castanopsis carlesii and Quercus griffithii.[Method] DNA fingerprints of C.hystrix,C.carlesii and Q.griffithii were established by using ISSR-PCR method.Cluster Analysis was carried out by using UPGMA method based on Nei＇s genetic distances among each individual.[Result] Six polymorphic primers were selected from 50 ISSR primers for ISSR-PCR amplification,and totally 86 discernible DNA bands were amplified with 53 polymorphic bands,accounting for 61.2% of the total.The average number of DNA bands amplified by each primer was 10.75.Specifically,totally 5 primers had amplified differential bands and specific bands,which were able to accurately identify C.hystrix,C.carlesii and Q.griffithii.As calculated by DPS v3.01 software,the genetic distances among test materials were ranged from 0.166 67 to 0.809 52,with an average of 0.563 57.[Conclusion] ISSR-PCR method can be used to identify C.hystrix,C.carlesii and Q.griffithii effectively.
文摘[Objective] The aim of this study was to provide a basis for distinguishing quality of rhubarb in different production areas. [Method ] X-ray diffraction patterns of rhubarbs in different production areas of Qinghai were obtained by X-ray diffraction analysis, and then its similarity analysis was also investigated. [ Result] The content of chemical components in rhubarbs from different production areas had differences, but its diffraction patterns and diffraction peaks had certain fingerprint characteristics. [ Conclusion] X-ray diffraction method is a fast and effective method for identifying rhubarb and other Chinese herbal medicines in different production areas.
文摘Aim To study the correlation between the HPLC fingerprints and in vitro antibacterial activities of EtOAc extracts of Radix isatidis from various sources. Methods Ten batches of Radix isatidis EtOAc extracts were analyzed with HPLC and the fingerprints were established. The influence of EtOAc extracts on the thermogenic curve of growth of Escherchia coli was obtained by microcalorimetry. The chemical differences of EtOAc extracts of Radix isatidis from various sources in the HPLC fingerprints were probed with hierarchical clustering analysis and similarity analysis. The correlation between the HPLC fingerprints and in vitro antibacterial activities was analyzed with multivafiant correlation analysis. Results Close correlation existed between the HPLC fingerprints and in vitro antibacterial activities of EtOAc extracts of Radix isatidis. Conlusion The combination of HPLC fingerprints and antibacterial activities can be used to discover principle components of Radix isatidis on bioactivity.
文摘The flavonoids is a kind of complex compound and quercetin is a main composition in rape pollen.Taking quercetin as a sample and using separation technology and high performance liquid chromatography,chromatography fingerprint technique of Chinese medicine is introduced in food field to construct chromatography fingerprint of rape pollen,which can be used for medicinal use and edible use.The method will become a beneficial supplement for quality control method to ensure the safety and effectiveness of products.
基金Supported by the Fund for Talented Scholars of Sichuan Academy of Agricultural SciencesSpecial Fund for New Subjects from the Innovation Capability Promotion Project of Sichuan Provincial Department of Finance(2013xxxk-020)~~
文摘In the present study, the nutritional compositions of maca which was grown in a mountain area at an elevation of 2 200-2 800 m of Ebian County,Sichuan Province were measured, and then HPLC analysis on two representative active compounds（macaene and macamide） in the maca sample was performed.The results revealed that there were 24.20% total protein, 18.40% total amino acids（including 3.84% arginine）, 42.80% total sugars, 1.36% fat and kinds of minerals（including 1.14% potassium） in Ebian maca. HPLC fingerprints of macaene and macamide of Ebian maca were similar to those of Peru maca. The results suggested that maca could be cultivated with good quality in some mountain areas with an altitude1 000 m lower than the origin place in Peru.
基金Supported by the Science and Technology Project of Zunyi City (Zunyi Science Cooperation[2016]12)Key Lab Construction Project of the Educational Department of Guizhou Province (Project No.:Guizhou Education Cooperation KY[2014]212)the Key Special Project for the Modernization of Chinese Traditional Medicine of Guizhou Province (Guizhou Science Cooperation[2012]6010)~~
文摘By analyzing the contents of inorganic elements in Radix Pseudostellariae from Guizhou Province, the aim was to assess the present quality of Radix Pseu- dostellariae, set limited standards of heavy metals, establish element fingerprints, and find out the characteristic elements. ICP-MS was used to measure the content of inorganic elements and map the element fingerprints. Additionally, WM/T2-2004 was applied to evaluate the quality of heavy metal elements, and the characteristic elements were determined by principal component analysis. The results showed that the contents of inorganic elements in Radix Pseudostellariae were between 0.057 and 959 mg/kg with the coefficients of variation ranging from 0.134 to 1.478, and the contents of Cd, As, Pb, and Hg were below the Standard of WM/T2-2004 in 90% of Radix Pseudostellariae. The standard limits of heavy metals in Radix Pseu- dostellariae were Cr≤6.5 mg/kg, Cu≤10 mg/kg, As≤2.0 mg /kg, Cd≤0.3 mg/kg, Hg≤0.15 mg/kg, and Pb≤4.0 mg/kg. The features of the inorganic eJements finger- prints could provide theoretical basis of identifying the quality of Radix Pseudostel- lariae and distinguishing Radix Pseudostellariae from other Chinese herbal medicines. The characteristic inorganic elements of Radix Pseudostellariae were found to be Cd, Cu, Co, Zn, Fe, Ca, Mg, and AI. Radix Pseudostellariae from Guizhou Province contained abundant inorganic elements, and the contents of heavy metals were below the evaluation criterion. The study provided a reference for the future development of the limiting values of heaw metals in Radix Pseudostellariae.
文摘This paper reports the rationale and methods of DNA extraction and purification from nine species of Compositae and four commercial drugs of corresponding plant Elephantopus scaber. The comparison of three methods: CsCl gradient, CTAB/CsCl gradient and CTAB miniprep extraction by yield, purity and factors affecting PCR was carried out. In conclusion, CTAB miniprep method provides a rapid, effective, economic approach for isolating genomic DNA for Chinese drug identification by genomic fingerprints.
基金Program for Changjiang Scholar and InnovativeTeam in University(Grant No.985-2-063-112) National Supporting Program for TCM from Ministry of Science andTechnology of China(Grant No.2006BAI08B03-03).
文摘Aim To establish a method for differentiating commercial samples of Panax species including notoginseng, cultivated ginseng （Chinese ginseng and Korean ginseng）, wild ginseng, red ginseng, three types of American ginsengs, and one American ginseng preparation with their HPLC fingerprints for assnrning the quality of different commercial samples of Panax species. Methods HPLC-UV method was used to establish their fingerprints, Zorbax Extend C18 （250 mm×4.6 mm, 5 μm） was used as the analytical column, and acetonitrile/KH2PO4 aqueous solution was used as the mobile phase with gradient elution. Results The fingerprints of different commercial samples of Panax species varied in their holistic chromatograms and some specific constituents. Conclusion This method is reliable, reproducible and simple, It could be applied in the routine authentication of different commercial samples of Panax species
