以盘基网柄菌为研究对象,首先建立基于成簇的规律间隔短回文重复序列及其相关蛋白9(clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9,CRISPR/Cas9)基因编辑系统的erkA基因敲除方法,DNA测序和蛋...以盘基网柄菌为研究对象,首先建立基于成簇的规律间隔短回文重复序列及其相关蛋白9(clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9,CRISPR/Cas9)基因编辑系统的erkA基因敲除方法,DNA测序和蛋白质印迹实验都证明所设计靶点都能有效地编辑erkA;随后对erkA突变株的趋电性进行探究,发现在12 V/cm的直流电场中,erkA突变株仍维持着与野生型细胞接近的趋电性,仅在运动速度上表现出增加趋势.在盘基网柄菌中利用CRISPR/Cas9系统成功构建了基因突变株,也明确了在盘基网柄菌中erkA基因并不是细胞感应电场的关键基因.展开更多
The electrochemical reaction of xanthate on the surface of pyrite was studied using cyclic voltammogrametry, chronopotentiometry and rotating-disc electrode measurements.Experimental results demonstrate that the first...The electrochemical reaction of xanthate on the surface of pyrite was studied using cyclic voltammogrametry, chronopotentiometry and rotating-disc electrode measurements.Experimental results demonstrate that the first step in the reaction is electrochemical adsorption of xanthate ion,and then the adsorbed ion associates with a xanthate ion from the solution and forms a dixanthogen on the pyrite electrode surface.The diffusion coefficient of butyl xanthate on pyrite electrode surface can be determined to be about 1.09×10- 6cm2/s.Using the galvanostatic technique,the kinetic parameters of oxidation of the butyl xanthate ion on the pyrite surface are calculated as Ja=200μA/cm2 ,β=0.203 and J0=27.1μA/cm2.展开更多
神经嵴（neural crest,NC）是一种具有高度迁移能力的多功能细胞群,它形成于胚胎发育过程中神经上皮和上皮细胞前体之间的交界处。神经嵴细胞在经历了横贯整个胚胎的迁移之后,会固定下来并分化发育成多种组织和器官。神经嵴细胞在迁移...神经嵴（neural crest,NC）是一种具有高度迁移能力的多功能细胞群,它形成于胚胎发育过程中神经上皮和上皮细胞前体之间的交界处。神经嵴细胞在经历了横贯整个胚胎的迁移之后,会固定下来并分化发育成多种组织和器官。神经嵴细胞在迁移过程中表现出趋化性（chemotaxis）和趋电性（electrotaxis）。神经嵴细胞能够沿着胞外可溶性因子浓度梯度产生定向迁移,这些趋化性因子包括SDF-1、VEGE、FGF、PDGF等;神经嵴细胞也能在生理电场（endogenous electric fields）或适当外源电场（exogenous electric fields）中沿电场方向,向正极或负极迁移。一些重要的与趋电性相关的分子已经被发现,如EGFR、Rac1、V-ATPase H＋pump、PI3 kinase/Pten。本综述详细介绍了神经嵴细胞趋化性和趋电性迁移中的可能机理和实验证据,为后续研究提供参考。展开更多
文摘以盘基网柄菌为研究对象,首先建立基于成簇的规律间隔短回文重复序列及其相关蛋白9(clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9,CRISPR/Cas9)基因编辑系统的erkA基因敲除方法,DNA测序和蛋白质印迹实验都证明所设计靶点都能有效地编辑erkA;随后对erkA突变株的趋电性进行探究,发现在12 V/cm的直流电场中,erkA突变株仍维持着与野生型细胞接近的趋电性,仅在运动速度上表现出增加趋势.在盘基网柄菌中利用CRISPR/Cas9系统成功构建了基因突变株,也明确了在盘基网柄菌中erkA基因并不是细胞感应电场的关键基因.
基金Project(50204013)supported by the National Natural Science Foundation of China
文摘The electrochemical reaction of xanthate on the surface of pyrite was studied using cyclic voltammogrametry, chronopotentiometry and rotating-disc electrode measurements.Experimental results demonstrate that the first step in the reaction is electrochemical adsorption of xanthate ion,and then the adsorbed ion associates with a xanthate ion from the solution and forms a dixanthogen on the pyrite electrode surface.The diffusion coefficient of butyl xanthate on pyrite electrode surface can be determined to be about 1.09×10- 6cm2/s.Using the galvanostatic technique,the kinetic parameters of oxidation of the butyl xanthate ion on the pyrite surface are calculated as Ja=200μA/cm2 ,β=0.203 and J0=27.1μA/cm2.
文摘神经嵴（neural crest,NC）是一种具有高度迁移能力的多功能细胞群,它形成于胚胎发育过程中神经上皮和上皮细胞前体之间的交界处。神经嵴细胞在经历了横贯整个胚胎的迁移之后,会固定下来并分化发育成多种组织和器官。神经嵴细胞在迁移过程中表现出趋化性（chemotaxis）和趋电性（electrotaxis）。神经嵴细胞能够沿着胞外可溶性因子浓度梯度产生定向迁移,这些趋化性因子包括SDF-1、VEGE、FGF、PDGF等;神经嵴细胞也能在生理电场（endogenous electric fields）或适当外源电场（exogenous electric fields）中沿电场方向,向正极或负极迁移。一些重要的与趋电性相关的分子已经被发现,如EGFR、Rac1、V-ATPase H＋pump、PI3 kinase/Pten。本综述详细介绍了神经嵴细胞趋化性和趋电性迁移中的可能机理和实验证据,为后续研究提供参考。
