定理:三角形任何两边的和大于第三边。推论:三角形任何两边的差小于第三边。把它们推广:动点 A 与两点B、C,当 A 不在直线 BC 上时,由上述定理及推论,必有|AB|+|AC|>|BC| ‖AB|-|AC‖<|BC|,当点 A 在线段BC 上时,|AB|+|AC|=|BC|,...定理:三角形任何两边的和大于第三边。推论:三角形任何两边的差小于第三边。把它们推广:动点 A 与两点B、C,当 A 不在直线 BC 上时,由上述定理及推论,必有|AB|+|AC|>|BC| ‖AB|-|AC‖<|BC|,当点 A 在线段BC 上时,|AB|+|AC|=|BC|,如图(1)。展开更多
Objective: We investigated the expression levels of KiSS-1 and KAI-1 in gallbladder carcinoma and their relationship with clinicopathologic parameters and metastasis potential. Methods: Pathological specimens from 59 ...Objective: We investigated the expression levels of KiSS-1 and KAI-1 in gallbladder carcinoma and their relationship with clinicopathologic parameters and metastasis potential. Methods: Pathological specimens from 59 gallbladder carcinoma tissues (including 23 invasion tissues), matched 7 para-tumor and 6 normal gallbladder tissues were examined for the expression of KiSS-1 and KAI-1 protein by tissue microarray technique and immunohistochemistry (EnVision). Results: The positive rate of KiSS-1 and KAI-1 was down-regulated (P < 0.05) in tumor tissues, compared with non-tumor tissues. The positive rate of KiSS-1 and KAI-1 were down-regulated (P < 0.01) in invasion tissues, compared with para-tumor tissues. Down-regulating expression levels of KiSS-1 and KAI-1 were significantly correlated with some malignant behavior of gallbladder carcinoma. The positive rate of KiSS-1 and KAI-1 were all negatively correlated with the Nevin staging of gallbladder carcinoma (P < 0.01). Moreover, a strong positive correlation was found between the positive expression of KiSS-1 and KAI-1 in gallbladder carcinoma (P < 0.001). It was indicated that KiSS-1 and KAI-1 were all associated with high metastasis potential of gallbladder carcinoma. Conclusion: KiSS-1 and/or KAI-1 may be important markers of reflecting invasive and metastatic potential and prognosis in gallbladder carcinoma, and may be the target genes to inhibit metastasis of gallbladder carcinoma. Detection of the expression of KiSS-1 and/or KAI-1 may have important clinical value for finding early-stage and evaluating the prognosis of gallbladder carcinoma.展开更多
This study investigated the effect of heat treatment combined with acid and alkali on the angiotensin-I- converting enzyme （ACE） inhibitory activity of peptides derived from bovine casein. The free amino group conte...This study investigated the effect of heat treatment combined with acid and alkali on the angiotensin-I- converting enzyme （ACE） inhibitory activity of peptides derived from bovine casein. The free amino group content, color, and cytotoxicity of the peptides were measured under different conditions. When heated at 100 ℃ in the pH range from 9.0 to 12.0, ACE inhibitory activity was reduced and the appearance of the peptides was significantly darkened. After thermal treatment in the presence of acid and alkali, the free amino group content of ACE inhibitory peptides decreased markedly. High temperature and prolonged heating also resulted in the loss of ACE inhibitory activity, the loss of free amino groups, and the darker coloration of bovine casein-derived peptides. However, ACE inhibitory peptides, within a concentration range of from 0.01 to 0.2 mg/ml, showed no cytotoxicity to Caco-2 and ECV-304 cell lines after heat treatment. This indicated that high temperature and alkaline heat treatment impaired the stability of bovine casein-derived ACE inhibitory peptides.展开更多
基金Supported by a grant from the Technology Program of Social Development Foundation of Kunshan (No. KS0703)
文摘Objective: We investigated the expression levels of KiSS-1 and KAI-1 in gallbladder carcinoma and their relationship with clinicopathologic parameters and metastasis potential. Methods: Pathological specimens from 59 gallbladder carcinoma tissues (including 23 invasion tissues), matched 7 para-tumor and 6 normal gallbladder tissues were examined for the expression of KiSS-1 and KAI-1 protein by tissue microarray technique and immunohistochemistry (EnVision). Results: The positive rate of KiSS-1 and KAI-1 was down-regulated (P < 0.05) in tumor tissues, compared with non-tumor tissues. The positive rate of KiSS-1 and KAI-1 were down-regulated (P < 0.01) in invasion tissues, compared with para-tumor tissues. Down-regulating expression levels of KiSS-1 and KAI-1 were significantly correlated with some malignant behavior of gallbladder carcinoma. The positive rate of KiSS-1 and KAI-1 were all negatively correlated with the Nevin staging of gallbladder carcinoma (P < 0.01). Moreover, a strong positive correlation was found between the positive expression of KiSS-1 and KAI-1 in gallbladder carcinoma (P < 0.001). It was indicated that KiSS-1 and KAI-1 were all associated with high metastasis potential of gallbladder carcinoma. Conclusion: KiSS-1 and/or KAI-1 may be important markers of reflecting invasive and metastatic potential and prognosis in gallbladder carcinoma, and may be the target genes to inhibit metastasis of gallbladder carcinoma. Detection of the expression of KiSS-1 and/or KAI-1 may have important clinical value for finding early-stage and evaluating the prognosis of gallbladder carcinoma.
基金supported by the Innovative Research Team of Higher Education of Heilongjiang Province(No.2010td11)the National Natural Science Foundation of China(No.31000801)+1 种基金the National Key Technology R&D Program of China during the 12th Five-Year Plan Period(No.2013BAD18B06)the 2009 Doctoral Science Research of Northeast Agricultural University,China
文摘This study investigated the effect of heat treatment combined with acid and alkali on the angiotensin-I- converting enzyme （ACE） inhibitory activity of peptides derived from bovine casein. The free amino group content, color, and cytotoxicity of the peptides were measured under different conditions. When heated at 100 ℃ in the pH range from 9.0 to 12.0, ACE inhibitory activity was reduced and the appearance of the peptides was significantly darkened. After thermal treatment in the presence of acid and alkali, the free amino group content of ACE inhibitory peptides decreased markedly. High temperature and prolonged heating also resulted in the loss of ACE inhibitory activity, the loss of free amino groups, and the darker coloration of bovine casein-derived peptides. However, ACE inhibitory peptides, within a concentration range of from 0.01 to 0.2 mg/ml, showed no cytotoxicity to Caco-2 and ECV-304 cell lines after heat treatment. This indicated that high temperature and alkaline heat treatment impaired the stability of bovine casein-derived ACE inhibitory peptides.
