[ Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone （GH） protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A. sinensis as the base...[ Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone （GH） protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A. sinensis as the base, the secondary structure of GH protein from A. sinensis was predicted by the method of Gamier-Robson, Chou-Fasman and Karpius-Schulz, and its cell epitope was predicted by the method of Kyte- Doolittle, Emini and Jameson-Wolf. [Result] The sections of 18 -23, 55 -55, 67 -73, 83 -86,112 -114,151 -157 and 209 -211 in the N terminal of GH protein molecule had softer structure and these sections could sway or fold to produce more complex tertiary structure. The sections of 19 -23, 51 -71,84 -95, 128 -139, 164 -176 and 189 -195 in the N terminal of GH protein could be the epitope of B cell and there were flexible regions in these sections or near these sections of GH protein molecule. So the dominant regions could be in these sections or near these sections. [ Conclusion] The research provided the basis for the preparation of monoctonal antibody of GH protein from A. sinensis and provided the reference for the discussion for the molecular regulation mechanism of A. sinensis.展开更多
A genetic linkage map of Pacific abalone(Haliotis discus hannai) was constructed using AFLP markers based on a two-way pseudo-testcross strategy in a full-sib family. With 33 primer combinations,a total of 455 markers...A genetic linkage map of Pacific abalone(Haliotis discus hannai) was constructed using AFLP markers based on a two-way pseudo-testcross strategy in a full-sib family. With 33 primer combinations,a total of 455 markers(225 from the female parent and 230 from the male parent) segregated in a 1:1 ratio,corresponding to DNA polymorphism:heterozygous in one parent and null in the other. The female framework map consisted of 174 markers distributed in 18 linkage groups,equivalent to the H. discus hannai haploid chromosome number,and spanning a total length of 2031.4 cM,with an average interval of 13.0 cM between adjacent markers. The male framework map consisted of 195 markers mapped on 19 linkage groups,spanning a total length of 2273.4 cM,with an average spacing of 12.9 cM between adjacent markers. The estimated coverage for the framework linkage maps was 81.2% for the female and 82.1% for the male,on the basis of two estimates of genome length. Fifty-two markers(11.4%) remained unlinked. The level of segregation distortion observed in this cross was 20.4%. These linkage maps will serve as a starting point for linkage studies in the Pacific abalone with potential application for marker-assisted selection in breeding programs.展开更多
Chiton(Ischnochiton hakodadensis) is one of marine mollusks well known for its eight separate shell plates. I. hakodadensis is important, which plays a vital role in the ecosystems it inhabits. So far, the genetic stu...Chiton(Ischnochiton hakodadensis) is one of marine mollusks well known for its eight separate shell plates. I. hakodadensis is important, which plays a vital role in the ecosystems it inhabits. So far, the genetic studies on the chiton are scarce due in part to insufficient genomic resources available for this species. In this study, we investigated the transcriptome of the chiton foot using Illumina sequencing technology. The reads were assembled and clustered into 256461 unigenes, of which 42247 were divided into diverse functional categories by Gene Ontology(GO) annotation terms, and 17256 mapped onto 365 pathways by KEGG pathway mapping. Meanwhile, a set of differentially expressed genes(DEGs) between distal and proximal muscles were identified as the foot adhesive locomotion associated, thus were useful for our future studies. Moreover, up to 679384 high-quality single nucleotide polymorphisms(SNPs) and 19814 simple sequence repeats(SSRs) were identified in this study, which are valuable for subsequent studies on genetic diversity and variation. The transcriptomic resource obtained in this study should aid to future genetic and genomic studies of chiton.展开更多
Three new species of Family Mitridae (Mollusca: Gastropoda) from the South China Sea are described in the present paper. They are Ziba aglais sp. nov. B. LI & S. ZHANG, Neocancilla daidaleosa sp. nov. B. LI & ...Three new species of Family Mitridae (Mollusca: Gastropoda) from the South China Sea are described in the present paper. They are Ziba aglais sp. nov. B. LI & S. ZHANG, Neocancilla daidaleosa sp. nov. B. LI & X. LI, and Mitra holkosa sp. nov. B. LI. Their systematic positions are also discussed.展开更多
Elements （vanadium and zinc） were determined in the two most abundant species of shell mollusks （Unio tigridis and Fiviparous bengalensis） and the water where they live in five stations in the Iraqi marshes. The s...Elements （vanadium and zinc） were determined in the two most abundant species of shell mollusks （Unio tigridis and Fiviparous bengalensis） and the water where they live in five stations in the Iraqi marshes. The study of concentrations elements was a target organ for aquatic environmental. Comparison between the mean concentrations of the elements in the water concentration levels of all studied elements was the highest with the mollusks. The results show that the station 5 is where the highest concentrations are found in water and in the two species. There is a significant correlation （P = 0.224） for concentrations of vanadium and P = 0.735 for zinc in two species of mollusks relative to their concentrations in surface water. All significant differences were accepted at a level of P 〈 0.05. The potential uptake of elements including V and Zn, as Viviparous bengalensi 〉 Unio tigridis, The mechanism for elements uptake determined by water for organisms confined to the contaminated areas seemed to be largely independent of species, mollusks demonstrated a significant positive relationship with water. These results have important implications for the utility of marshes biota as subjects for elements monitoring program.展开更多
Spionid worms of Polydora ciliata inhabit the shells of many commercially important bivalves and cause disease in molluscan aquaculture. Their sperm structure is closely related to their fertilization method. To give ...Spionid worms of Polydora ciliata inhabit the shells of many commercially important bivalves and cause disease in molluscan aquaculture. Their sperm structure is closely related to their fertilization method. To give an insight into the sperm structure and spermatogenesis, ultrastructure details of the subcellular components of germ cells during spermiogenesis of Polydora ciliata are detected by transmission electron microscopy(TEM). In P. ciliata, during spermiogenesis, chromatin is regularly arranged as dense fibrils and becomes more condensed when the nucleus elongates. Microtubules do not surround the nucleus during its elongation. The Golgi phase is characterized by the formation of proacrosomal granules within the Golgi apparatus. The proacrosomal granules fuse to form a single, spherical acrosomal vesicle that migrates to the anterior pole of the cell. At the time of nuclear condensation, mitochondria become reduced in number but increased in size, causing deep indentation at the base of the nucleus. The mid-piece has a few mitochondria. The cap phase includes the spreading of the acrosomal granule over the surface of the nucleus of the differentiating spermatid. The acrosomal phase of spermiogenesis is typically associated with changes in the shape of the nucleus, acrosome and tail. The relationship of sperm ultrastructure to spermiogenesis in spionidae species was discussed.展开更多
A brief information about two subspecies mollusks in the bank of Amu Darya river is collected according to the experience. As a result, we identified 9 types and 2 small types of two sub species mollusks live in Amu D...A brief information about two subspecies mollusks in the bank of Amu Darya river is collected according to the experience. As a result, we identified 9 types and 2 small types of two sub species mollusks live in Amu Darya river. Sinanodonta gibba, Sinanodonta orbicularis and Sinanodonta puerorum types for fauna were shown for the first time from the river.展开更多
Allochthonous organic matter plays an important role in nutrient cycling and energy mobilization in freshwater ecosystems. However, the subsidies of this carbon source in floodplain ecosystems have not yet well unders...Allochthonous organic matter plays an important role in nutrient cycling and energy mobilization in freshwater ecosystems. However, the subsidies of this carbon source in floodplain ecosystems have not yet well understood. We used a Bayesian mixing model and stable isotopes （613C and 815N） of primary food resources and dominant molluscs species, to estimate the relative importance of allochthonous carbon sources for consumers in a representative sub-lake of Poyang Lake during a prolonged dry season. Our study inferred that terrestrial-derived carbon from Carex spp. could be the primary contributor to snails and mussels in Dahuchi Lake. The mean percentage of allochthonous food resources accounted for 35%- 50% of the C incorporated by these consumers. Seston was another important energy sources for benthic consumers. However, during the winter and low water-level period, benthic algae and submerged vegetation contributed less carbon to benthic consumers. Our data highlighted the importance of terrestrial organic carbon to benthic consumers in the wetlands of Poyang Lake during the prolonged dry period. Further, our results provided a perspective that linkages between terrestrial and aquatic ecosystems might be facilitated by wintering geese via their droppings.展开更多
In this paper, investigation has been done in the computer simulation of the electrostatic coupling IBC by using the developed finite-element models, in which a.the incidence and reflection of electronic signal in the...In this paper, investigation has been done in the computer simulation of the electrostatic coupling IBC by using the developed finite-element models, in which a.the incidence and reflection of electronic signal in the upper arm model were analyzed by using the theory of electromagnetic wave;b.the finite-element models of electrostatic coupling IBC were developed by using the electromagnetic analysis package of ANSYS software;c.the signal attenuation of electrostatic coupling IBC were simulated under the conditions of different signal frequencies, electrodes directions, electrodes sizes and transmission distances. Finally, some important conclusions are deduced on the basis of simulation results.展开更多
The present study gives a detailed analysis of HM （heavy metals） accumulation in mollusks Nodularia （Bivalvia, Unionidae） from the transboundary part of the Amur River （Russia-China）. This river basin experience...The present study gives a detailed analysis of HM （heavy metals） accumulation in mollusks Nodularia （Bivalvia, Unionidae） from the transboundary part of the Amur River （Russia-China）. This river basin experiences different levels of toxic pollution. Differences in the rate of HM bioaccumulation in mollusks are identified depending on their size, sex （male and female）, content of HM in environment, and ratio of accumulating macro- and micro-components in tissue. Mollusks＇ response to the change of HM content in the environment is expressed in the different intensities of their accumulation in tissue. The possibility of estimating toxic danger for mollusk according to the index of HM bioaccumulation with consideration of frequency of pathologies is shown. These indexes permitted estimation of the ecotoxicological condition of the mollusks population in the parts of the Amur, upstream and downstream of the inflow of the Sungari River and before and after the accidental pollution, respectively. It is found out that mollusks are able for bioremediation when pollution of the aquatic environment decreases. Quantitative correlation of HM bioaccumulation index in mollusks to HM content in the environment makes it possible to forecast the population condition, and to substantiate the necessity of ecological control of anthropogenic load on the ecosystem.展开更多
Mollusc shell matrix proteins （SMPs） are important functional components embedded in the shell and play a role in shell formation. A SMP （Pif177） was identified previously from the nacreous layer of the Japanese p...Mollusc shell matrix proteins （SMPs） are important functional components embedded in the shell and play a role in shell formation. A SMP （Pif177） was identified previously from the nacreous layer of the Japanese pearl oyster Pinctadafucata, and its cleavage products （named pfPif97 and pfPif80 proteins） were found to bind to the chitin framework and induce aragonite crystal formation and orient the c axis. In this study, a homologue of pfPif177 was cloned from the mantle of the Pacific oyster Crassostrea gigas, containing the homologue of pfPif97 only and not pfPif80. This finding hints at the large divergence in gene structure between the two species. This homologue （cgPif97） shares characteristics with pfPif97, and suggests that the biological functions of these two proteins may be similar. The expression pattern of cgPif97 in different tissues and development stages in- dicates that it may play an important role in shell formation of the adult oyster. The morphology of the inner shell surface was af- fected by injected siRNA of cgPif97 and the calcite laths of the shell became thinner and narrower when the siRNA dose in- creased, suggesting that the cgPip7 gene plays an important role in calcite shell formation in C. gigas. In conclusion, we found evidence that the Pif177 gene evolved very fast but still retains a similar function among species [Current Zoology 59 （1）： 109-115, 2013].展开更多
文摘[ Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone （GH） protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A. sinensis as the base, the secondary structure of GH protein from A. sinensis was predicted by the method of Gamier-Robson, Chou-Fasman and Karpius-Schulz, and its cell epitope was predicted by the method of Kyte- Doolittle, Emini and Jameson-Wolf. [Result] The sections of 18 -23, 55 -55, 67 -73, 83 -86,112 -114,151 -157 and 209 -211 in the N terminal of GH protein molecule had softer structure and these sections could sway or fold to produce more complex tertiary structure. The sections of 19 -23, 51 -71,84 -95, 128 -139, 164 -176 and 189 -195 in the N terminal of GH protein could be the epitope of B cell and there were flexible regions in these sections or near these sections of GH protein molecule. So the dominant regions could be in these sections or near these sections. [ Conclusion] The research provided the basis for the preparation of monoctonal antibody of GH protein from A. sinensis and provided the reference for the discussion for the molecular regulation mechanism of A. sinensis.
基金The study was supported by grants from the Chinese Ministry of Education(NCET-04-0640)the National Natural Science Foundation of China(No.30571442).
文摘A genetic linkage map of Pacific abalone(Haliotis discus hannai) was constructed using AFLP markers based on a two-way pseudo-testcross strategy in a full-sib family. With 33 primer combinations,a total of 455 markers(225 from the female parent and 230 from the male parent) segregated in a 1:1 ratio,corresponding to DNA polymorphism:heterozygous in one parent and null in the other. The female framework map consisted of 174 markers distributed in 18 linkage groups,equivalent to the H. discus hannai haploid chromosome number,and spanning a total length of 2031.4 cM,with an average interval of 13.0 cM between adjacent markers. The male framework map consisted of 195 markers mapped on 19 linkage groups,spanning a total length of 2273.4 cM,with an average spacing of 12.9 cM between adjacent markers. The estimated coverage for the framework linkage maps was 81.2% for the female and 82.1% for the male,on the basis of two estimates of genome length. Fifty-two markers(11.4%) remained unlinked. The level of segregation distortion observed in this cross was 20.4%. These linkage maps will serve as a starting point for linkage studies in the Pacific abalone with potential application for marker-assisted selection in breeding programs.
基金grant support from the National Natural Science Foundation of China (Nos. 31130054, 31472258)the AoShan Talents Program of Qingdao National Laboratory for Marine Science and Technology (No. 2015ASTP-ES02)the Fundamental Research Funds for the Central Universities (No. 201564009)
文摘Chiton(Ischnochiton hakodadensis) is one of marine mollusks well known for its eight separate shell plates. I. hakodadensis is important, which plays a vital role in the ecosystems it inhabits. So far, the genetic studies on the chiton are scarce due in part to insufficient genomic resources available for this species. In this study, we investigated the transcriptome of the chiton foot using Illumina sequencing technology. The reads were assembled and clustered into 256461 unigenes, of which 42247 were divided into diverse functional categories by Gene Ontology(GO) annotation terms, and 17256 mapped onto 365 pathways by KEGG pathway mapping. Meanwhile, a set of differentially expressed genes(DEGs) between distal and proximal muscles were identified as the foot adhesive locomotion associated, thus were useful for our future studies. Moreover, up to 679384 high-quality single nucleotide polymorphisms(SNPs) and 19814 simple sequence repeats(SSRs) were identified in this study, which are valuable for subsequent studies on genetic diversity and variation. The transcriptomic resource obtained in this study should aid to future genetic and genomic studies of chiton.
基金The study supported by the Natural Science Foundation of China (39899400 and 40276044), project of Chinese Academy of Sciences (KSCX2-SW-101B) and IOCAS for innovation (L57022807).
文摘Three new species of Family Mitridae (Mollusca: Gastropoda) from the South China Sea are described in the present paper. They are Ziba aglais sp. nov. B. LI & S. ZHANG, Neocancilla daidaleosa sp. nov. B. LI & X. LI, and Mitra holkosa sp. nov. B. LI. Their systematic positions are also discussed.
文摘Elements （vanadium and zinc） were determined in the two most abundant species of shell mollusks （Unio tigridis and Fiviparous bengalensis） and the water where they live in five stations in the Iraqi marshes. The study of concentrations elements was a target organ for aquatic environmental. Comparison between the mean concentrations of the elements in the water concentration levels of all studied elements was the highest with the mollusks. The results show that the station 5 is where the highest concentrations are found in water and in the two species. There is a significant correlation （P = 0.224） for concentrations of vanadium and P = 0.735 for zinc in two species of mollusks relative to their concentrations in surface water. All significant differences were accepted at a level of P 〈 0.05. The potential uptake of elements including V and Zn, as Viviparous bengalensi 〉 Unio tigridis, The mechanism for elements uptake determined by water for organisms confined to the contaminated areas seemed to be largely independent of species, mollusks demonstrated a significant positive relationship with water. These results have important implications for the utility of marshes biota as subjects for elements monitoring program.
基金supported by the National Key Technology Support Program (2011BAD13B05)
文摘Spionid worms of Polydora ciliata inhabit the shells of many commercially important bivalves and cause disease in molluscan aquaculture. Their sperm structure is closely related to their fertilization method. To give an insight into the sperm structure and spermatogenesis, ultrastructure details of the subcellular components of germ cells during spermiogenesis of Polydora ciliata are detected by transmission electron microscopy(TEM). In P. ciliata, during spermiogenesis, chromatin is regularly arranged as dense fibrils and becomes more condensed when the nucleus elongates. Microtubules do not surround the nucleus during its elongation. The Golgi phase is characterized by the formation of proacrosomal granules within the Golgi apparatus. The proacrosomal granules fuse to form a single, spherical acrosomal vesicle that migrates to the anterior pole of the cell. At the time of nuclear condensation, mitochondria become reduced in number but increased in size, causing deep indentation at the base of the nucleus. The mid-piece has a few mitochondria. The cap phase includes the spreading of the acrosomal granule over the surface of the nucleus of the differentiating spermatid. The acrosomal phase of spermiogenesis is typically associated with changes in the shape of the nucleus, acrosome and tail. The relationship of sperm ultrastructure to spermiogenesis in spionidae species was discussed.
文摘A brief information about two subspecies mollusks in the bank of Amu Darya river is collected according to the experience. As a result, we identified 9 types and 2 small types of two sub species mollusks live in Amu Darya river. Sinanodonta gibba, Sinanodonta orbicularis and Sinanodonta puerorum types for fauna were shown for the first time from the river.
基金Supported by the National Natural Science Foundation of China(Nos.41471088,41301077)
文摘Allochthonous organic matter plays an important role in nutrient cycling and energy mobilization in freshwater ecosystems. However, the subsidies of this carbon source in floodplain ecosystems have not yet well understood. We used a Bayesian mixing model and stable isotopes （613C and 815N） of primary food resources and dominant molluscs species, to estimate the relative importance of allochthonous carbon sources for consumers in a representative sub-lake of Poyang Lake during a prolonged dry season. Our study inferred that terrestrial-derived carbon from Carex spp. could be the primary contributor to snails and mussels in Dahuchi Lake. The mean percentage of allochthonous food resources accounted for 35%- 50% of the C incorporated by these consumers. Seston was another important energy sources for benthic consumers. However, during the winter and low water-level period, benthic algae and submerged vegetation contributed less carbon to benthic consumers. Our data highlighted the importance of terrestrial organic carbon to benthic consumers in the wetlands of Poyang Lake during the prolonged dry period. Further, our results provided a perspective that linkages between terrestrial and aquatic ecosystems might be facilitated by wintering geese via their droppings.
基金National Natural Science Foundation of China ( No. 60801050)the Basic Research Foundationof Beijing Institute of Technology of China ( No. 1010050320804) National Innovation Experiment Program for University Students( 2010)
文摘In this paper, investigation has been done in the computer simulation of the electrostatic coupling IBC by using the developed finite-element models, in which a.the incidence and reflection of electronic signal in the upper arm model were analyzed by using the theory of electromagnetic wave;b.the finite-element models of electrostatic coupling IBC were developed by using the electromagnetic analysis package of ANSYS software;c.the signal attenuation of electrostatic coupling IBC were simulated under the conditions of different signal frequencies, electrodes directions, electrodes sizes and transmission distances. Finally, some important conclusions are deduced on the basis of simulation results.
文摘The present study gives a detailed analysis of HM （heavy metals） accumulation in mollusks Nodularia （Bivalvia, Unionidae） from the transboundary part of the Amur River （Russia-China）. This river basin experiences different levels of toxic pollution. Differences in the rate of HM bioaccumulation in mollusks are identified depending on their size, sex （male and female）, content of HM in environment, and ratio of accumulating macro- and micro-components in tissue. Mollusks＇ response to the change of HM content in the environment is expressed in the different intensities of their accumulation in tissue. The possibility of estimating toxic danger for mollusk according to the index of HM bioaccumulation with consideration of frequency of pathologies is shown. These indexes permitted estimation of the ecotoxicological condition of the mollusks population in the parts of the Amur, upstream and downstream of the inflow of the Sungari River and before and after the accidental pollution, respectively. It is found out that mollusks are able for bioremediation when pollution of the aquatic environment decreases. Quantitative correlation of HM bioaccumulation index in mollusks to HM content in the environment makes it possible to forecast the population condition, and to substantiate the necessity of ecological control of anthropogenic load on the ecosystem.
文摘Mollusc shell matrix proteins （SMPs） are important functional components embedded in the shell and play a role in shell formation. A SMP （Pif177） was identified previously from the nacreous layer of the Japanese pearl oyster Pinctadafucata, and its cleavage products （named pfPif97 and pfPif80 proteins） were found to bind to the chitin framework and induce aragonite crystal formation and orient the c axis. In this study, a homologue of pfPif177 was cloned from the mantle of the Pacific oyster Crassostrea gigas, containing the homologue of pfPif97 only and not pfPif80. This finding hints at the large divergence in gene structure between the two species. This homologue （cgPif97） shares characteristics with pfPif97, and suggests that the biological functions of these two proteins may be similar. The expression pattern of cgPif97 in different tissues and development stages in- dicates that it may play an important role in shell formation of the adult oyster. The morphology of the inner shell surface was af- fected by injected siRNA of cgPif97 and the calcite laths of the shell became thinner and narrower when the siRNA dose in- creased, suggesting that the cgPip7 gene plays an important role in calcite shell formation in C. gigas. In conclusion, we found evidence that the Pif177 gene evolved very fast but still retains a similar function among species [Current Zoology 59 （1）： 109-115, 2013].
