Hybridoma cells were cultured by continuous perfusion in Fibra-Cel of 5L packed-bed bioreactor for 22 days in low serum or serum-free media.The corresponded amino acids were fed and serum concentration was decreased b...Hybridoma cells were cultured by continuous perfusion in Fibra-Cel of 5L packed-bed bioreactor for 22 days in low serum or serum-free media.The corresponded amino acids were fed and serum concentration was decreased by analyzing glucose concentration, oxygen uptake rate, secretary antibody amount and amino acids concentration in culture supernatant. Comparing with continuous perfusion culture that amino acids were not fed, antibody amunt of production was increased about 2～3 times. The inoculated cell density was 2.5×10 5 cells/mL,while the final cell density was 8.79×10 8cells/mL. Antibody production was reached 295mg/L/d at average level, and the highest level was reached 532mg/L/d.These results provided a primary mode of enlarge culture for monoclonal antibody industrilization.展开更多
A Super\|Spinner was Modified by mounting a stainless steel filter(pore size 75 μm)to the impeller shaft to retain cells while fresh nutrient is perfused.Using Macroporous microcarrier Cytopore 1,continuously perfuse...A Super\|Spinner was Modified by mounting a stainless steel filter(pore size 75 μm)to the impeller shaft to retain cells while fresh nutrient is perfused.Using Macroporous microcarrier Cytopore 1,continuously perfused cultivation of a recombinant CHO cell line,CHO2DS producing prothrombin was performed with the perfusion of a protein\|free medium DF6S.The cell retention rate was more than 90% during the 24 days continuously perfused cultivation.The viable cell density of CHO2DS and prothrombin concentration reached 4.62×10 6(cells.m/L) and 11.3(mg/L)respectively after 9 days culture.展开更多
文摘Hybridoma cells were cultured by continuous perfusion in Fibra-Cel of 5L packed-bed bioreactor for 22 days in low serum or serum-free media.The corresponded amino acids were fed and serum concentration was decreased by analyzing glucose concentration, oxygen uptake rate, secretary antibody amount and amino acids concentration in culture supernatant. Comparing with continuous perfusion culture that amino acids were not fed, antibody amunt of production was increased about 2～3 times. The inoculated cell density was 2.5×10 5 cells/mL,while the final cell density was 8.79×10 8cells/mL. Antibody production was reached 295mg/L/d at average level, and the highest level was reached 532mg/L/d.These results provided a primary mode of enlarge culture for monoclonal antibody industrilization.
文摘A Super\|Spinner was Modified by mounting a stainless steel filter(pore size 75 μm)to the impeller shaft to retain cells while fresh nutrient is perfused.Using Macroporous microcarrier Cytopore 1,continuously perfused cultivation of a recombinant CHO cell line,CHO2DS producing prothrombin was performed with the perfusion of a protein\|free medium DF6S.The cell retention rate was more than 90% during the 24 days continuously perfused cultivation.The viable cell density of CHO2DS and prothrombin concentration reached 4.62×10 6(cells.m/L) and 11.3(mg/L)respectively after 9 days culture.
