The research on the architecture and urbanism of the former Portuguese Africa allows us to consider and to establish the real intrinsic value, typological and morphological diversity, and the lengthy duration of the l...The research on the architecture and urbanism of the former Portuguese Africa allows us to consider and to establish the real intrinsic value, typological and morphological diversity, and the lengthy duration of the legacy of this architectural/urban heritage. Portuguese colonization in Africa produced more recently, mostly in the period 1950-1975, some vast and great material values, of which seven or eight main cities, including the vast contents of their modern architecture, are striking examples. We can evaluate the most positive aspects with future impact represented by this Portuguese architects＇ practice （cultural ethics, use of modern technology, and service to society） and the architectonic work produced during that era. Although these cities and its architecture were created within a late colonial context of indisputable discrimination, they have prevailed despite wars and circumstantial abandonment. Now they represent a consistent material basis for the modernization and/or revitalization of urban life in these countries. Architects that are at present working and intervening in these countries should understand this ambivalence and complementary nature between the ex-colonial ＂concrete city＂ and the ＂informal city＂ pre- and post-colonial, so that their performance is socially and culturally informed, correct, and positive.展开更多
The passive flight of large numbers of mitospores ensures propagation and survival of many fungal species. Moreover, many fungi have a surplus DNA context which allows for easier manipulation and tampering, especially...The passive flight of large numbers of mitospores ensures propagation and survival of many fungal species. Moreover, many fungi have a surplus DNA context which allows for easier manipulation and tampering, especially when inserting eukaryotic genes and translation systems. These facts taken together suggest a high prominence of the sporogonic airborne fungi in biosecurity/biodefence priority lists, but due to the low virulence and mortality of the fungal pathogens, they do not figure high, or, sometimes, not at all. The disruptive effect of biosecurity fungal threats is important per se and genetic manipulations are to further it. Novel, long range and high-persistence sampling methods, using robotics and unmanned systems already fielded in other applications may extend surveillance and responsive sampling. Effects-based, proteomic approaches may provide a solution for deliberate incidents, while polyphasic taxonomy may resolve spontaneous incidents and assist medical diagnosis, but in the biosecurity context, where mass collection and processing of samples and speed in producing robust results are vital, complex approaches might be proved more of a hindrance than an asset.展开更多
A growing number of genes responsible for reproductive incompatibilities between species （barrier loci） exhibit the signals of positive selection. However, the possibility that genes experiencing positive selection ...A growing number of genes responsible for reproductive incompatibilities between species （barrier loci） exhibit the signals of positive selection. However, the possibility that genes experiencing positive selection diverge early in speciation and commonly cause reproductive incompatibilities has not been systematically investigated on a genome-wide scale. Here, I outline a research program for studying the genetic basis of speciation in broadcast spawning marine invertebrates that uses a priori genome-wide information on a large, unbiased sample of genes tested for positive selection. A targeted sequence capture approach is proposed that scores single-nucleotide polymorphisms （SNPs） in widely separated species populations at an early stage of allopatric divergence. The targeted capture of both coding and non-coding sequences enables SNPs to be characterized at known locations across the genome and at genes with known selective or neutral histories. The neutral coding and non-coding SNPs provide robust background distributions for identifying Fsm-outliers within genes that can, in principle, identify specific mutations experiencing diversifying selection. If natural hybridization occurs between species, the neutral coding and noncoding SNPs can provide a neutral admixture model for genomic clines analyses aimed at finding genes exhibiting strong blocks to introgression. Strongylocentrotid sea urchins are used as a model system to outline the approach but it can be used for any group that has a complete reference genome available.展开更多
文摘The research on the architecture and urbanism of the former Portuguese Africa allows us to consider and to establish the real intrinsic value, typological and morphological diversity, and the lengthy duration of the legacy of this architectural/urban heritage. Portuguese colonization in Africa produced more recently, mostly in the period 1950-1975, some vast and great material values, of which seven or eight main cities, including the vast contents of their modern architecture, are striking examples. We can evaluate the most positive aspects with future impact represented by this Portuguese architects＇ practice （cultural ethics, use of modern technology, and service to society） and the architectonic work produced during that era. Although these cities and its architecture were created within a late colonial context of indisputable discrimination, they have prevailed despite wars and circumstantial abandonment. Now they represent a consistent material basis for the modernization and/or revitalization of urban life in these countries. Architects that are at present working and intervening in these countries should understand this ambivalence and complementary nature between the ex-colonial ＂concrete city＂ and the ＂informal city＂ pre- and post-colonial, so that their performance is socially and culturally informed, correct, and positive.
文摘The passive flight of large numbers of mitospores ensures propagation and survival of many fungal species. Moreover, many fungi have a surplus DNA context which allows for easier manipulation and tampering, especially when inserting eukaryotic genes and translation systems. These facts taken together suggest a high prominence of the sporogonic airborne fungi in biosecurity/biodefence priority lists, but due to the low virulence and mortality of the fungal pathogens, they do not figure high, or, sometimes, not at all. The disruptive effect of biosecurity fungal threats is important per se and genetic manipulations are to further it. Novel, long range and high-persistence sampling methods, using robotics and unmanned systems already fielded in other applications may extend surveillance and responsive sampling. Effects-based, proteomic approaches may provide a solution for deliberate incidents, while polyphasic taxonomy may resolve spontaneous incidents and assist medical diagnosis, but in the biosecurity context, where mass collection and processing of samples and speed in producing robust results are vital, complex approaches might be proved more of a hindrance than an asset.
基金Acknowledgments I would like to thank Nicolas Bierne for the opportunity of contributing to the Special Column： Population Genomics in the Sea. Helpful comments on the manuscript were provided by Nicolas Bierne and two anonymous reviewers.Partial funding for the work described on strongylocentrotid sea urchins was provided by the Natinal Science Foundation （DEB-1011061 ）.
文摘A growing number of genes responsible for reproductive incompatibilities between species （barrier loci） exhibit the signals of positive selection. However, the possibility that genes experiencing positive selection diverge early in speciation and commonly cause reproductive incompatibilities has not been systematically investigated on a genome-wide scale. Here, I outline a research program for studying the genetic basis of speciation in broadcast spawning marine invertebrates that uses a priori genome-wide information on a large, unbiased sample of genes tested for positive selection. A targeted sequence capture approach is proposed that scores single-nucleotide polymorphisms （SNPs） in widely separated species populations at an early stage of allopatric divergence. The targeted capture of both coding and non-coding sequences enables SNPs to be characterized at known locations across the genome and at genes with known selective or neutral histories. The neutral coding and non-coding SNPs provide robust background distributions for identifying Fsm-outliers within genes that can, in principle, identify specific mutations experiencing diversifying selection. If natural hybridization occurs between species, the neutral coding and noncoding SNPs can provide a neutral admixture model for genomic clines analyses aimed at finding genes exhibiting strong blocks to introgression. Strongylocentrotid sea urchins are used as a model system to outline the approach but it can be used for any group that has a complete reference genome available.
